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The map label for this gene is fusA2 [C]

Identifier: 148659884

GI number: 148659884

Start: 146986

End: 149130

Strand: Reverse

Name: fusA2 [C]

Synonym: MRA_0127

Alternate gene names: 148659884

Gene position: 149130-146986 (Counterclockwise)

Preceding gene: 148659885

Following gene: 148659882

Centisome position: 3.37

GC content: 65.92

Gene sequence:

>2145_bases
ATGGCCGACAGAGTGAATGCTTCCCAGGGCGCGGCCGCCGCTCCCACCGCAAACGGGCCCGGCGGCGTTCGCAACGTGGT
TCTGGTGGGGCCCTCGGGGGGCGGCAAGACCACCCTCATCGAGGCTCTCCTGGTGGCGGCCAAGGTGTTGTCCAGGCCGG
GCTCGGTCACCGAGGGCACCACGGTCTGCGACTTCGACGAGGCGGAGATCCGGCAACAGCGCTCGGTGGGCCTTGCGGTC
GCCTCGCTGGCTTACGACGGCATCAAGGTCAACCTCGTCGACACCCCTGGGTACGCCGACTTCGTGGGTGAGCTGCGGGC
CGGGTTGCGGGCCGCCGATTGCGCACTGTTCGTGATCGCGGCCAACGAGGGCGTCGACGAACCGACCAAGTCCCTGTGGC
AGGAATGCAGCCAGGTCGGCATGCCCCGCGCGGTGGTCATCACCAAGCTGGACCACGCCCGGGCGAACTACCGGGAAGCG
CTGACCGCGGCACAAGACGCCTTCGGCGACAAGGTCTTACCGCTCTACCTGCCGTCCGGTGACGGCCTGATCGGATTGCT
TTCGCAGGCACTGTACGAGTACGCCGACGGCAAGCGAACGACACGAACCCCGGCCGAGTCGGACACCGAGCGAATCGAGG
AGGCGCGCGGCGCCTTGATCGAAGGAATCATCGAGGAATCCGAGGATGAGTCCTTGATGGAGCGCTACCTCGGCGGCGAG
ACGATCGACGAGTCGGTGCTGATCCAGGATCTGGAGAAAGCCGTCGCCCGAGGCTCGTTCTTCCCGGTGATTCCGGTGTG
CAGCAGCACCGGGGTCGGCACACTGGAATTGCTTGAGGTAGCCACCCGCGGATTTCCGTCTCCGATGGAGCACCCACTGC
CGGAGGTCTTTACCCCGCAGGGTGTCCCGCATGCGGAGCTGGCCTGTGACAATGACGCGCCGTTGCTCGCCGAGGTGGTG
AAGACGACGTCGGACCCCTACGTCGGCCGGGTCAGCTTGGTGCGGGTGTTCTCCGGAACCATCAGGCCCGACACGACGGT
CCATGTGTCGGGCCATTTCTCATCGTTTTTCGGCGGCGGAACTAGTAACACGCACCCCGACCACGACGAAGACGAGCGCA
TCGGAGTCTTGTCGTTCCCGCTGGGCAAGCAACAGCGCCCGGCAGCAGCCGTGGTCGCGGGCGACATCTGCGCCATCGGC
AAGCTGAGCCGGGCCGAGACCGGGGACACACTCTCGGATAAGGCCGAGCCCCTGGTGCTGAAACCCTGGACCATGCCCGA
GCCGCTGCTGCCGATCGCCATTGCGGCGCATGCCAAGACCGACGAAGACAAGCTGTCGGTCGGGTTGGGCCGGTTGGCCG
CCGAAGACCCGACACTGCGGATCGAGCAAAATCAAGAGACCCATCAGGTCGTGCTGTGGTGTATGGGCGAGGCCCATGCC
GGCGTCGTCCTGGATACACTGGCCAACCGGTACGGCGTCAGCGTAGACACGATCGAGCTGCGGGTGCCGCTGCGGGAAAC
CTTCGCCGGCAATGCGAAAGGCCATGGCCGCCACATCAAACAGTCCGGTGGTCACGGTCAGTACGGGGTGTGCGACATCG
AGGTGGAGCCGCTGCCGGAGGGCTCCGGGTTCGAGTTCCTCGACAAAGTGGTTGGCGGGGCGGTGCCGCGGCAGTTTATC
CCCAATGTGGAGAAAGGTGTCCGCGCACAGATGGATAAGGGAGTGCACGCCGGCTACCCGGTGGTCGATATCCGGGTCAC
GTTGCTCGACGGCAAAGCCCACAGCGTCGACTCTTCCGATTTCGCCTTCCAAATGGCCGGCGCGCTCGCATTGCGGGAGG
CCGCGGCCGCGACGAAGGTGATCTTGCTCGAGCCAATCGATGAGATTTCGGTGCTGGTACCCGACGATTTCGTCGGCGCG
GTGTTGGGCGACCTGTCCAGTCGTCGTGGCCGAGTGCTCGGCACCGAGACCGCGGGGCACGACCGCACGGTGATCAAGGC
CGAGGTGCCTCAGGTCGAGCTGACCCGCTACGCAATCGATTTGCGCTCGCTGGCGCATGGGGCCGCATCCTTCACCCGTT
CGTTTGCCCGCTACGAACCGATGCCGGAATCCGCCGCCGCCCGGGTAAAGGCCGGGGCCGGCTAG

Upstream 100 bases:

>100_bases
ACCCGACCCTTGTGCTGGGCGGCAGTTGGGAGAAAAGTTGCCTACTACGTCCTGCTGGGCCACACCACGGGAACCGACCC
CGGAGAACTGGAGGAAAGCC

Downstream 100 bases:

>100_bases
CTACACCGAATGGCCGAACCGTTCGGCGACAGCGCGGCGGTCGAGCGAACCCTTCGCGGTGTGCGGCAGCCCGCTGGCCT
CCTGGAAGGAGGCCGGGATC

Product: elongation factor G

Products: GDP; phosphate

Alternate protein names: NA

Number of amino acids: Translated: 714; Mature: 713

Protein sequence:

>714_residues
MADRVNASQGAAAAPTANGPGGVRNVVLVGPSGGGKTTLIEALLVAAKVLSRPGSVTEGTTVCDFDEAEIRQQRSVGLAV
ASLAYDGIKVNLVDTPGYADFVGELRAGLRAADCALFVIAANEGVDEPTKSLWQECSQVGMPRAVVITKLDHARANYREA
LTAAQDAFGDKVLPLYLPSGDGLIGLLSQALYEYADGKRTTRTPAESDTERIEEARGALIEGIIEESEDESLMERYLGGE
TIDESVLIQDLEKAVARGSFFPVIPVCSSTGVGTLELLEVATRGFPSPMEHPLPEVFTPQGVPHAELACDNDAPLLAEVV
KTTSDPYVGRVSLVRVFSGTIRPDTTVHVSGHFSSFFGGGTSNTHPDHDEDERIGVLSFPLGKQQRPAAAVVAGDICAIG
KLSRAETGDTLSDKAEPLVLKPWTMPEPLLPIAIAAHAKTDEDKLSVGLGRLAAEDPTLRIEQNQETHQVVLWCMGEAHA
GVVLDTLANRYGVSVDTIELRVPLRETFAGNAKGHGRHIKQSGGHGQYGVCDIEVEPLPEGSGFEFLDKVVGGAVPRQFI
PNVEKGVRAQMDKGVHAGYPVVDIRVTLLDGKAHSVDSSDFAFQMAGALALREAAAATKVILLEPIDEISVLVPDDFVGA
VLGDLSSRRGRVLGTETAGHDRTVIKAEVPQVELTRYAIDLRSLAHGAASFTRSFARYEPMPESAAARVKAGAG

Sequences:

>Translated_714_residues
MADRVNASQGAAAAPTANGPGGVRNVVLVGPSGGGKTTLIEALLVAAKVLSRPGSVTEGTTVCDFDEAEIRQQRSVGLAV
ASLAYDGIKVNLVDTPGYADFVGELRAGLRAADCALFVIAANEGVDEPTKSLWQECSQVGMPRAVVITKLDHARANYREA
LTAAQDAFGDKVLPLYLPSGDGLIGLLSQALYEYADGKRTTRTPAESDTERIEEARGALIEGIIEESEDESLMERYLGGE
TIDESVLIQDLEKAVARGSFFPVIPVCSSTGVGTLELLEVATRGFPSPMEHPLPEVFTPQGVPHAELACDNDAPLLAEVV
KTTSDPYVGRVSLVRVFSGTIRPDTTVHVSGHFSSFFGGGTSNTHPDHDEDERIGVLSFPLGKQQRPAAAVVAGDICAIG
KLSRAETGDTLSDKAEPLVLKPWTMPEPLLPIAIAAHAKTDEDKLSVGLGRLAAEDPTLRIEQNQETHQVVLWCMGEAHA
GVVLDTLANRYGVSVDTIELRVPLRETFAGNAKGHGRHIKQSGGHGQYGVCDIEVEPLPEGSGFEFLDKVVGGAVPRQFI
PNVEKGVRAQMDKGVHAGYPVVDIRVTLLDGKAHSVDSSDFAFQMAGALALREAAAATKVILLEPIDEISVLVPDDFVGA
VLGDLSSRRGRVLGTETAGHDRTVIKAEVPQVELTRYAIDLRSLAHGAASFTRSFARYEPMPESAAARVKAGAG
>Mature_713_residues
ADRVNASQGAAAAPTANGPGGVRNVVLVGPSGGGKTTLIEALLVAAKVLSRPGSVTEGTTVCDFDEAEIRQQRSVGLAVA
SLAYDGIKVNLVDTPGYADFVGELRAGLRAADCALFVIAANEGVDEPTKSLWQECSQVGMPRAVVITKLDHARANYREAL
TAAQDAFGDKVLPLYLPSGDGLIGLLSQALYEYADGKRTTRTPAESDTERIEEARGALIEGIIEESEDESLMERYLGGET
IDESVLIQDLEKAVARGSFFPVIPVCSSTGVGTLELLEVATRGFPSPMEHPLPEVFTPQGVPHAELACDNDAPLLAEVVK
TTSDPYVGRVSLVRVFSGTIRPDTTVHVSGHFSSFFGGGTSNTHPDHDEDERIGVLSFPLGKQQRPAAAVVAGDICAIGK
LSRAETGDTLSDKAEPLVLKPWTMPEPLLPIAIAAHAKTDEDKLSVGLGRLAAEDPTLRIEQNQETHQVVLWCMGEAHAG
VVLDTLANRYGVSVDTIELRVPLRETFAGNAKGHGRHIKQSGGHGQYGVCDIEVEPLPEGSGFEFLDKVVGGAVPRQFIP
NVEKGVRAQMDKGVHAGYPVVDIRVTLLDGKAHSVDSSDFAFQMAGALALREAAAATKVILLEPIDEISVLVPDDFVGAV
LGDLSSRRGRVLGTETAGHDRTVIKAEVPQVELTRYAIDLRSLAHGAASFTRSFARYEPMPESAAARVKAGAG

Specific function: This Protein Promotes The GTP-Dependent Translocation Of The Nascent Protein Chain From The A-Site To The P-Site Of The Ribosome. [C]

COG id: COG0480

COG function: function code J; Translation elongation factors (GTPases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily

Homologues:

Organism=Homo sapiens, GI18390331, Length=704, Percent_Identity=28.6931818181818, Blast_Score=260, Evalue=3e-69,
Organism=Homo sapiens, GI19923640, Length=732, Percent_Identity=26.0928961748634, Blast_Score=192, Evalue=1e-48,
Organism=Homo sapiens, GI25306287, Length=359, Percent_Identity=25.3481894150418, Blast_Score=111, Evalue=3e-24,
Organism=Homo sapiens, GI25306283, Length=414, Percent_Identity=27.2946859903382, Blast_Score=108, Evalue=1e-23,
Organism=Escherichia coli, GI1789738, Length=710, Percent_Identity=32.3943661971831, Blast_Score=353, Evalue=3e-98,
Organism=Escherichia coli, GI1790835, Length=330, Percent_Identity=24.5454545454545, Blast_Score=75, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17533571, Length=714, Percent_Identity=26.750700280112, Blast_Score=239, Evalue=5e-63,
Organism=Caenorhabditis elegans, GI17556745, Length=156, Percent_Identity=31.4102564102564, Blast_Score=92, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI71988819, Length=152, Percent_Identity=31.5789473684211, Blast_Score=67, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI71988811, Length=152, Percent_Identity=31.5789473684211, Blast_Score=66, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6323098, Length=698, Percent_Identity=25.5014326647564, Blast_Score=210, Evalue=7e-55,
Organism=Saccharomyces cerevisiae, GI6322359, Length=826, Percent_Identity=23.728813559322, Blast_Score=177, Evalue=4e-45,
Organism=Drosophila melanogaster, GI24582462, Length=697, Percent_Identity=29.2682926829268, Blast_Score=256, Evalue=3e-68,
Organism=Drosophila melanogaster, GI221458488, Length=748, Percent_Identity=22.8609625668449, Blast_Score=160, Evalue=3e-39,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 7984 Molecules/Cell In: Growth Phase, Gl

Swissprot (AC and ID): EFGL_MYCTU (O07170)

Other databases:

- EMBL:   BX842572
- EMBL:   AE000516
- PIR:   A70983
- RefSeq:   NP_214634.1
- RefSeq:   NP_334538.1
- ProteinModelPortal:   O07170
- SMR:   O07170
- EnsemblBacteria:   EBMYCT00000001508
- EnsemblBacteria:   EBMYCT00000070236
- GeneID:   886894
- GeneID:   922988
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT0128
- KEGG:   mtu:Rv0120c
- TIGR:   MT0128
- TubercuList:   Rv0120c
- GeneTree:   EBGT00050000015348
- HOGENOM:   HBG737692
- OMA:   AFIFKTM
- ProtClustDB:   PRK12740
- InterPro:   IPR003593
- InterPro:   IPR009022
- InterPro:   IPR000795
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR005517
- InterPro:   IPR004161
- InterPro:   IPR009000
- Gene3D:   G3DSA:3.30.230.10
- Gene3D:   G3DSA:3.30.70.240
- SMART:   SM00382
- SMART:   SM00838
- SMART:   SM00889
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00679 EFG_C; PF03764 EFG_IV; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; SSF54980 EFG_III_V; SSF54211 Ribosomal_S5_D2-typ_fold; SSF50447 Translat_factor

EC number: 3.6.5.3

Molecular weight: Translated: 75631; Mature: 75500

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADRVNASQGAAAAPTANGPGGVRNVVLVGPSGGGKTTLIEALLVAAKVLSRPGSVTEGT
CCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC
TVCDFDEAEIRQQRSVGLAVASLAYDGIKVNLVDTPGYADFVGELRAGLRAADCALFVIA
EEECCCHHHHHHHHHHHHHHHHEEECCEEEEEEECCCHHHHHHHHHHCCEECCEEEEEEE
ANEGVDEPTKSLWQECSQVGMPRAVVITKLDHARANYREALTAAQDAFGDKVLPLYLPSG
CCCCCCHHHHHHHHHHHHCCCCEEEEEEECHHHHHHHHHHHHHHHHHCCCCEEEEEECCC
DGLIGLLSQALYEYADGKRTTRTPAESDTERIEEARGALIEGIIEESEDESLMERYLGGE
CCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCC
TIDESVLIQDLEKAVARGSFFPVIPVCSSTGVGTLELLEVATRGFPSPMEHPLPEVFTPQ
CCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCC
GVPHAELACDNDAPLLAEVVKTTSDPYVGRVSLVRVFSGTIRPDTTVHVSGHFSSFFGGG
CCCCCCEECCCCCHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCEEEEECCHHHHCCCC
TSNTHPDHDEDERIGVLSFPLGKQQRPAAAVVAGDICAIGKLSRAETGDTLSDKAEPLVL
CCCCCCCCCCCCCEEEEECCCCCCCCCCCEEEECCHHHHCCCCCCCCCCCCCCCCCCEEE
KPWTMPEPLLPIAIAAHAKTDEDKLSVGLGRLAAEDPTLRIEQNQETHQVVLWCMGEAHA
CCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCEEEECCCCCEEEEEEEECCCCC
GVVLDTLANRYGVSVDTIELRVPLRETFAGNAKGHGRHIKQSGGHGQYGVCDIEVEPLPE
CHHHHHHHHHHCCEEEEEEEEECHHHHHCCCCCCCCCHHHCCCCCCCCEEEEEEEECCCC
GSGFEFLDKVVGGAVPRQFIPNVEKGVRAQMDKGVHAGYPVVDIRVTLLDGKAHSVDSSD
CCCHHHHHHHHCCCCCHHHCCCHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCCCCCH
FAFQMAGALALREAAAATKVILLEPIDEISVLVPDDFVGAVLGDLSSRRGRVLGTETAGH
HHHHHHHHHHHHHHHHHEEEEEECCCCCCEEECCCHHHHHHHHHHHHCCCCEEECCCCCC
DRTVIKAEVPQVELTRYAIDLRSLAHGAASFTRSFARYEPMPESAAARVKAGAG
CCEEEEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCC
>Mature Secondary Structure 
ADRVNASQGAAAAPTANGPGGVRNVVLVGPSGGGKTTLIEALLVAAKVLSRPGSVTEGT
CCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC
TVCDFDEAEIRQQRSVGLAVASLAYDGIKVNLVDTPGYADFVGELRAGLRAADCALFVIA
EEECCCHHHHHHHHHHHHHHHHEEECCEEEEEEECCCHHHHHHHHHHCCEECCEEEEEEE
ANEGVDEPTKSLWQECSQVGMPRAVVITKLDHARANYREALTAAQDAFGDKVLPLYLPSG
CCCCCCHHHHHHHHHHHHCCCCEEEEEEECHHHHHHHHHHHHHHHHHCCCCEEEEEECCC
DGLIGLLSQALYEYADGKRTTRTPAESDTERIEEARGALIEGIIEESEDESLMERYLGGE
CCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCC
TIDESVLIQDLEKAVARGSFFPVIPVCSSTGVGTLELLEVATRGFPSPMEHPLPEVFTPQ
CCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCC
GVPHAELACDNDAPLLAEVVKTTSDPYVGRVSLVRVFSGTIRPDTTVHVSGHFSSFFGGG
CCCCCCEECCCCCHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCEEEEECCHHHHCCCC
TSNTHPDHDEDERIGVLSFPLGKQQRPAAAVVAGDICAIGKLSRAETGDTLSDKAEPLVL
CCCCCCCCCCCCCEEEEECCCCCCCCCCCEEEECCHHHHCCCCCCCCCCCCCCCCCCEEE
KPWTMPEPLLPIAIAAHAKTDEDKLSVGLGRLAAEDPTLRIEQNQETHQVVLWCMGEAHA
CCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCEEEECCCCCEEEEEEEECCCCC
GVVLDTLANRYGVSVDTIELRVPLRETFAGNAKGHGRHIKQSGGHGQYGVCDIEVEPLPE
CHHHHHHHHHHCCEEEEEEEEECHHHHHCCCCCCCCCHHHCCCCCCCCEEEEEEEECCCC
GSGFEFLDKVVGGAVPRQFIPNVEKGVRAQMDKGVHAGYPVVDIRVTLLDGKAHSVDSSD
CCCHHHHHHHHCCCCCHHHCCCHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCCCCCH
FAFQMAGALALREAAAATKVILLEPIDEISVLVPDDFVGAVLGDLSSRRGRVLGTETAGH
HHHHHHHHHHHHHHHHHEEEEEECCCCCCEEECCCHHHHHHHHHHHHCCCCEEECCCCCC
DRTVIKAEVPQVELTRYAIDLRSLAHGAASFTRSFARYEPMPESAAARVKAGAG
CCEEEEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036