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The map label for this gene is gca [H]

Identifier: 148659875

GI number: 148659875

Start: 137648

End: 138604

Strand: Direct

Name: gca [H]

Synonym: MRA_0118

Alternate gene names: 148659875

Gene position: 137648-138604 (Clockwise)

Preceding gene: 148659874

Following gene: 148659876

Centisome position: 3.11

GC content: 55.9

Gene sequence:

>957_bases
ATGAAAGTGTGGATCACTGGGGCTGGCGGAATGATGGGGTCACATCTCGCCGAAATGTTGCTGGCCGCCGGACACGATGT
GTACGCTACCTACTGCAGGCCGACCATCGATCCGTCGGACCTGCAATTCAACGGAGCAGAAGTCGATATCACCGACTGGT
GCTCGGTCTACGATTCGATAGCGACATTCCGCCCCGACGCGGTATTTCATCTCGCGGCCCAAAGCTATCCGGCGGTTTCG
TGGGCCCGGCCGGTTGAGACGCTGACCACCAACATGGTTGGCACCGCCATCGTTTTCGAAGCACTACGTCGCGTGCGACC
GCACGCAAAGATTATTGTTGCGGGCTCGTCGGCCGAATATGGATTTGTTGACCCATCCGAGGTTCCGATTAATGAGCGGC
GAGAACTTCGCCCGCTCCATCCGTATGGTGTTTCTAAGGCGGCCACCGACATGCTGGCGTATCAATATCACAAGTCTTAC
GGCATGCACACCGTCGTCGCTCGTATCTTCAATTGCACCGGGCCACGCAAAGTCGGAGATGCACTTTCCGATTTCGTCCG
CCGTTGTACATGGTTGGAGCACCATCCGGAACAAAGTGCCATCCGGGTGGGAAATCTTAAGACGAAACGGACTATCGTGG
ACGTCCGCGATCTCAATCGGGCGTTGATGCTGATGCTGGATAAAGGCGAGGCCGGGGCTGACTACAATGTGGGAGGTTCG
ATCGCCTACGAGATGGGCGACGTTCTCAAACAAGTAATCGCGGCTTGTAAACGTGACGATATCGTGCCGGAAGTCGACCC
CGCCCTTCTTCGGCCCACCGACGAAAAGATCATCTACGGAGATTGCAGCAAGCTGGCGGCCATAACAGGCTGGCAACAAG
AAATCTGTTTGACTCAGACGATTGCCGACATGTTCGATTATTGGCGTAGCAAATCCGAGTCCGCCCTGATGGTGTGA

Upstream 100 bases:

>100_bases
GGCGCGCGCAACGAGGTGCGCACTATCCATTCGAGGTGAACTGGACTCCTTGATGCTCAGGCCGGTGCGGTTTGTCGAGA
AAGGCGAATAGGAACAGTCC

Downstream 100 bases:

>100_bases
CCGAATGTCTTTGTCCTGCCAACCTGAGGAGCAGATAAGATTGACCGTAACGGACTCTCAGTATCGACAAAAGGTGTGCA
CCGCGAGAACTGCTGAGGAG

Product: putative GDP-D-mannose dehydratase

Products: NA

Alternate protein names: GDP-D-mannose dehydratase [H]

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MKVWITGAGGMMGSHLAEMLLAAGHDVYATYCRPTIDPSDLQFNGAEVDITDWCSVYDSIATFRPDAVFHLAAQSYPAVS
WARPVETLTTNMVGTAIVFEALRRVRPHAKIIVAGSSAEYGFVDPSEVPINERRELRPLHPYGVSKAATDMLAYQYHKSY
GMHTVVARIFNCTGPRKVGDALSDFVRRCTWLEHHPEQSAIRVGNLKTKRTIVDVRDLNRALMLMLDKGEAGADYNVGGS
IAYEMGDVLKQVIAACKRDDIVPEVDPALLRPTDEKIIYGDCSKLAAITGWQQEICLTQTIADMFDYWRSKSESALMV

Sequences:

>Translated_318_residues
MKVWITGAGGMMGSHLAEMLLAAGHDVYATYCRPTIDPSDLQFNGAEVDITDWCSVYDSIATFRPDAVFHLAAQSYPAVS
WARPVETLTTNMVGTAIVFEALRRVRPHAKIIVAGSSAEYGFVDPSEVPINERRELRPLHPYGVSKAATDMLAYQYHKSY
GMHTVVARIFNCTGPRKVGDALSDFVRRCTWLEHHPEQSAIRVGNLKTKRTIVDVRDLNRALMLMLDKGEAGADYNVGGS
IAYEMGDVLKQVIAACKRDDIVPEVDPALLRPTDEKIIYGDCSKLAAITGWQQEICLTQTIADMFDYWRSKSESALMV
>Mature_318_residues
MKVWITGAGGMMGSHLAEMLLAAGHDVYATYCRPTIDPSDLQFNGAEVDITDWCSVYDSIATFRPDAVFHLAAQSYPAVS
WARPVETLTTNMVGTAIVFEALRRVRPHAKIIVAGSSAEYGFVDPSEVPINERRELRPLHPYGVSKAATDMLAYQYHKSY
GMHTVVARIFNCTGPRKVGDALSDFVRRCTWLEHHPEQSAIRVGNLKTKRTIVDVRDLNRALMLMLDKGEAGADYNVGGS
IAYEMGDVLKQVIAACKRDDIVPEVDPALLRPTDEKIIYGDCSKLAAITGWQQEICLTQTIADMFDYWRSKSESALMV

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GDP-mannose 4,6-dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI42516563, Length=334, Percent_Identity=25.1497005988024, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI4504031, Length=341, Percent_Identity=23.4604105571848, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI7657641, Length=326, Percent_Identity=24.2331288343558, Blast_Score=86, Evalue=4e-17,
Organism=Escherichia coli, GI1788366, Length=342, Percent_Identity=25.7309941520468, Blast_Score=104, Evalue=7e-24,
Organism=Escherichia coli, GI1788353, Length=347, Percent_Identity=26.5129682997118, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI48994969, Length=320, Percent_Identity=25.625, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI133901786, Length=333, Percent_Identity=26.4264264264264, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI133901788, Length=333, Percent_Identity=26.4264264264264, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17539424, Length=333, Percent_Identity=26.4264264264264, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI133901790, Length=333, Percent_Identity=26.4264264264264, Blast_Score=108, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17539422, Length=333, Percent_Identity=26.4264264264264, Blast_Score=108, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17507723, Length=333, Percent_Identity=24.6246246246246, Blast_Score=98, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17539532, Length=333, Percent_Identity=24.3243243243243, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17568069, Length=327, Percent_Identity=23.8532110091743, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24158427, Length=335, Percent_Identity=24.4776119402985, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI21356223, Length=332, Percent_Identity=25.6024096385542, Blast_Score=93, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR006368
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.47 [H]

Molecular weight: Translated: 35281; Mature: 35281

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVWITGAGGMMGSHLAEMLLAAGHDVYATYCRPTIDPSDLQFNGAEVDITDWCSVYDSI
CEEEEECCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEECCCEEEHHHHHHHHHHH
ATFRPDAVFHLAAQSYPAVSWARPVETLTTNMVGTAIVFEALRRVRPHAKIIVAGSSAEY
HHCCCCHHEEEHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCC
GFVDPSEVPINERRELRPLHPYGVSKAATDMLAYQYHKSYGMHTVVARIFNCTGPRKVGD
CCCCCCCCCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHH
ALSDFVRRCTWLEHHPEQSAIRVGNLKTKRTIVDVRDLNRALMLMLDKGEAGADYNVGGS
HHHHHHHHHHHHHCCCCCHHEEECCCCHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCH
IAYEMGDVLKQVIAACKRDDIVPEVDPALLRPTDEKIIYGDCSKLAAITGWQQEICLTQT
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCEEEECCHHHHHHHCCCHHHHHHHHH
IADMFDYWRSKSESALMV
HHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MKVWITGAGGMMGSHLAEMLLAAGHDVYATYCRPTIDPSDLQFNGAEVDITDWCSVYDSI
CEEEEECCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEECCCEEEHHHHHHHHHHH
ATFRPDAVFHLAAQSYPAVSWARPVETLTTNMVGTAIVFEALRRVRPHAKIIVAGSSAEY
HHCCCCHHEEEHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCC
GFVDPSEVPINERRELRPLHPYGVSKAATDMLAYQYHKSYGMHTVVARIFNCTGPRKVGD
CCCCCCCCCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHH
ALSDFVRRCTWLEHHPEQSAIRVGNLKTKRTIVDVRDLNRALMLMLDKGEAGADYNVGGS
HHHHHHHHHHHHHCCCCCHHEEECCCCHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCH
IAYEMGDVLKQVIAACKRDDIVPEVDPALLRPTDEKIIYGDCSKLAAITGWQQEICLTQT
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCEEEECCHHHHHHHCCCHHHHHHHHH
IADMFDYWRSKSESALMV
HHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8548534; 10984043 [H]