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The map label for this gene is hycQ [H]

Identifier: 148659848

GI number: 148659848

Start: 95309

End: 96775

Strand: Direct

Name: hycQ [H]

Synonym: MRA_0090

Alternate gene names: 148659848

Gene position: 95309-96775 (Clockwise)

Preceding gene: 148659847

Following gene: 148659849

Centisome position: 2.16

GC content: 68.92

Gene sequence:

>1467_bases
ATGACCGGTTTGCTGCTTGCCGCGATCCTCGCACCGCTCGCCGCGTCAATCGCCTCCTTGATCACCGGGTGGCGACGCAC
GACGGCGACGCTCACCGCGCTGTCCGCCACGACGGTGCTGGCCTGCGCTGTGGCGATGGGGTTTTGGATGGGGTCGGGGG
CGCAGTTCGGGCTGGGCGGTCTGCTGCGCGCCGATGCGCTGACGGTGGTCATGCTCGTCGTCATCGGGATCGTCGGCACA
CTGGCCACCGCGGCGAGCATCGGCTACATCGACACCGAGCTGGCACACGGGCATATCGACGGACGTAGCGCTCGGCTGTA
TGGGGTGCTGACCCCGGCGTTTCTTTGCGCGATGGTTCTGGCGGTGTGCGCCAACAACATCGGCGTCATTTGGGTAGCGA
TCGAGGCCACCACGGTGATCACCGCGTTTCTGGTGGGGCATCGCCGCACCCGCACCGCGCTGGAAGCGACCTGGAAATAC
GTGGTGATCTGTTCGGTCGGGATCGCCGTCGCCTTCTTGGGTACCGTGCTGCTGTATTTCGCCGCGCGGGATTCCGGTGC
CGCTGCTGCCGGCGCGCTGAACCTCGATATCCTGGCCGAACACGCCGCCGGCCTAGACCCCGGGGTCGCTCGACTGGCCG
GCGGGTTGCTGCTCATCGGTTATGGCGCCAAGGCGGGCCTCTTCCCGTTTCACACCTGGCTGGCGGACGCGCACAGCCAA
GCCCCCGCACCGGTGTCCGCACTGATGAGCGGCGTGCTGCTGGCGGTGGCGTTCTCGGTGCTGATCCGATTGCGGCCGAT
CCTCGACGCGGTCAGCGGGCCCGCCTACCTGCGCAACGGGCTGCTCGTGGTCGGGTTGGCGACGCTGCTGGTGGCGGTGC
TGATGCTGACCGTGACCGGCGACGTCAAGCGGATGCTGGCCTACTCGTCGATGGAGCACATGGGCCTGATCGCGATCGCC
GCGGCCGCCGGCACGACATTGGCGATCGCCGCGCTGCTGCTGCACGTGCTCGCCCACGGGATCGGCAAGACCGTGCTGTT
TCTGGCGGGCGGTCAGCTGCAGGCCGCACACGACTCCACCGCCATCGCCGATATCACCGGCGTGATGCGACGGTCGCGGC
TGATCGGCGTGTCGTTTGCCGTCGGCCTGATCGTCCTGCTTGGCTTGCCGCCGTTCGCGATGTTCGCCAGCGAGCTGGCG
ATCGCGCGCTCATTGGCCAACGAGCGGCTGGCCTGGGTGCTGGGTGCGGCGCTGCTGCTGATCGCCATCGGTTTCACGGC
TCTGGCACGCAATTCCGGACGCATGCTGCTCGGCACCCCGGCGGCGGGCGCGCCGGCGATCACCGTGCCGGCCACCGCGG
CGGCGGCGTTGATGGTGGGCATCGTCGTCTCGGCGGCCCTCGGCATCACCGCGGGCCCACTCGCCGACCTGCTTGGCATC
GCCGCCAGCAACGTGGGTCTACCGTGA

Upstream 100 bases:

>100_bases
ACGTGCTGTTCGCGGTCATCGTGATCGGCGTGTTGACCGGCCGGCTGCGCCGCATTTTCGGCGATGCCGACCTGGACAAG
CTGCGGGAGTTGCGGGATTG

Downstream 100 bases:

>100_bases
TGAGTGCCAGCTGGCTGCGCCACCGGGTATCCGAGCGTGGACTGATAGCGACGGCCGAACAACTCTGGGCCGATTCGTTT
CGCCTGGCCCTGGTCGCTGC

Product: putative hydrogenase HycQ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 488; Mature: 487

Protein sequence:

>488_residues
MTGLLLAAILAPLAASIASLITGWRRTTATLTALSATTVLACAVAMGFWMGSGAQFGLGGLLRADALTVVMLVVIGIVGT
LATAASIGYIDTELAHGHIDGRSARLYGVLTPAFLCAMVLAVCANNIGVIWVAIEATTVITAFLVGHRRTRTALEATWKY
VVICSVGIAVAFLGTVLLYFAARDSGAAAAGALNLDILAEHAAGLDPGVARLAGGLLLIGYGAKAGLFPFHTWLADAHSQ
APAPVSALMSGVLLAVAFSVLIRLRPILDAVSGPAYLRNGLLVVGLATLLVAVLMLTVTGDVKRMLAYSSMEHMGLIAIA
AAAGTTLAIAALLLHVLAHGIGKTVLFLAGGQLQAAHDSTAIADITGVMRRSRLIGVSFAVGLIVLLGLPPFAMFASELA
IARSLANERLAWVLGAALLLIAIGFTALARNSGRMLLGTPAAGAPAITVPATAAAALMVGIVVSAALGITAGPLADLLGI
AASNVGLP

Sequences:

>Translated_488_residues
MTGLLLAAILAPLAASIASLITGWRRTTATLTALSATTVLACAVAMGFWMGSGAQFGLGGLLRADALTVVMLVVIGIVGT
LATAASIGYIDTELAHGHIDGRSARLYGVLTPAFLCAMVLAVCANNIGVIWVAIEATTVITAFLVGHRRTRTALEATWKY
VVICSVGIAVAFLGTVLLYFAARDSGAAAAGALNLDILAEHAAGLDPGVARLAGGLLLIGYGAKAGLFPFHTWLADAHSQ
APAPVSALMSGVLLAVAFSVLIRLRPILDAVSGPAYLRNGLLVVGLATLLVAVLMLTVTGDVKRMLAYSSMEHMGLIAIA
AAAGTTLAIAALLLHVLAHGIGKTVLFLAGGQLQAAHDSTAIADITGVMRRSRLIGVSFAVGLIVLLGLPPFAMFASELA
IARSLANERLAWVLGAALLLIAIGFTALARNSGRMLLGTPAAGAPAITVPATAAAALMVGIVVSAALGITAGPLADLLGI
AASNVGLP
>Mature_487_residues
TGLLLAAILAPLAASIASLITGWRRTTATLTALSATTVLACAVAMGFWMGSGAQFGLGGLLRADALTVVMLVVIGIVGTL
ATAASIGYIDTELAHGHIDGRSARLYGVLTPAFLCAMVLAVCANNIGVIWVAIEATTVITAFLVGHRRTRTALEATWKYV
VICSVGIAVAFLGTVLLYFAARDSGAAAAGALNLDILAEHAAGLDPGVARLAGGLLLIGYGAKAGLFPFHTWLADAHSQA
PAPVSALMSGVLLAVAFSVLIRLRPILDAVSGPAYLRNGLLVVGLATLLVAVLMLTVTGDVKRMLAYSSMEHMGLIAIAA
AAGTTLAIAALLLHVLAHGIGKTVLFLAGGQLQAAHDSTAIADITGVMRRSRLIGVSFAVGLIVLLGLPPFAMFASELAI
ARSLANERLAWVLGAALLLIAIGFTALARNSGRMLLGTPAAGAPAITVPATAAAALMVGIVVSAALGITAGPLADLLGIA
ASNVGLP

Specific function: Unknown

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Escherichia coli, GI1788831, Length=379, Percent_Identity=40.1055408970976, Blast_Score=248, Evalue=6e-67,
Organism=Escherichia coli, GI1788827, Length=359, Percent_Identity=27.5766016713092, Blast_Score=112, Evalue=7e-26,
Organism=Escherichia coli, GI1788829, Length=412, Percent_Identity=27.6699029126214, Blast_Score=102, Evalue=8e-23,
Organism=Escherichia coli, GI145693160, Length=490, Percent_Identity=24.4897959183673, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1788613, Length=356, Percent_Identity=26.9662921348315, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1788614, Length=373, Percent_Identity=29.4906166219839, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI2367154, Length=354, Percent_Identity=27.1186440677966, Blast_Score=83, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 49227; Mature: 49096

Theoretical pI: Translated: 9.47; Mature: 9.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGLLLAAILAPLAASIASLITGWRRTTATLTALSATTVLACAVAMGFWMGSGAQFGLGG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCH
LLRADALTVVMLVVIGIVGTLATAASIGYIDTELAHGHIDGRSARLYGVLTPAFLCAMVL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCEEEEHHHHHHHHHHHH
AVCANNIGVIWVAIEATTVITAFLVGHRRTRTALEATWKYVVICSVGIAVAFLGTVLLYF
HHHHCCCCEEEEEEHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AARDSGAAAAGALNLDILAEHAAGLDPGVARLAGGLLLIGYGAKAGLFPFHTWLADAHSQ
HCCCCCCCHHCCCHHHHHHHHHCCCCCHHHHHCCCEEEEECCCCCCCCHHHHHHHHHCCC
APAPVSALMSGVLLAVAFSVLIRLRPILDAVSGPAYLRNGLLVVGLATLLVAVLMLTVTG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHHH
DVKRMLAYSSMEHMGLIAIAAAAGTTLAIAALLLHVLAHGIGKTVLFLAGGQLQAAHDST
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEEEECCCEECCCCCH
AIADITGVMRRSRLIGVSFAVGLIVLLGLPPFAMFASELAIARSLANERLAWVLGAALLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAIGFTALARNSGRMLLGTPAAGAPAITVPATAAAALMVGIVVSAALGITAGPLADLLGI
HHHHHHHHHCCCCCEEEECCCCCCCEEECCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
AASNVGLP
HHHCCCCC
>Mature Secondary Structure 
TGLLLAAILAPLAASIASLITGWRRTTATLTALSATTVLACAVAMGFWMGSGAQFGLGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCH
LLRADALTVVMLVVIGIVGTLATAASIGYIDTELAHGHIDGRSARLYGVLTPAFLCAMVL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCEEEEHHHHHHHHHHHH
AVCANNIGVIWVAIEATTVITAFLVGHRRTRTALEATWKYVVICSVGIAVAFLGTVLLYF
HHHHCCCCEEEEEEHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AARDSGAAAAGALNLDILAEHAAGLDPGVARLAGGLLLIGYGAKAGLFPFHTWLADAHSQ
HCCCCCCCHHCCCHHHHHHHHHCCCCCHHHHHCCCEEEEECCCCCCCCHHHHHHHHHCCC
APAPVSALMSGVLLAVAFSVLIRLRPILDAVSGPAYLRNGLLVVGLATLLVAVLMLTVTG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHHH
DVKRMLAYSSMEHMGLIAIAAAAGTTLAIAALLLHVLAHGIGKTVLFLAGGQLQAAHDST
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEEEECCCEECCCCCH
AIADITGVMRRSRLIGVSFAVGLIVLLGLPPFAMFASELAIARSLANERLAWVLGAALLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAIGFTALARNSGRMLLGTPAAGAPAITVPATAAAALMVGIVVSAALGITAGPLADLLGI
HHHHHHHHHCCCCCEEEECCCCCCCEEECCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
AASNVGLP
HHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]