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The map label for this gene is narI [H]

Identifier: 148652480

GI number: 148652480

Start: 813956

End: 814681

Strand: Direct

Name: narI [H]

Synonym: PsycPRwf_0669

Alternate gene names: 148652480

Gene position: 813956-814681 (Clockwise)

Preceding gene: 148652479

Following gene: 148652481

Centisome position: 27.32

GC content: 45.59

Gene sequence:

>726_bases
GTGAATATTGCAGATCCTGGTGTACAGTCTTTGGCCAACTTAAACTGGCTACAAATTTTCTTATTTGGTATCTACCCCTA
CATTGCATTAGTGGTTGCCATCCTTGGTACTTGGGCGCGTTTTGACTTGTCACAGTACTCATGGAAGACCGGCTCTTCAC
AAATGCTGCGCAGTAAAGGTATGCGTGTTGCGAGTAACTTATTCCACGTGGGTATTATTGTTGTCCTGCTGGGTCACTTG
GTTGGTATGTTAACGCCGCACTTTATTTATGAGCCATTTATCAGCGCTGCACACAAGCAAATCCTAGCGGTGGTGGTCGG
TGGTATTGCAGGGGCAGTGTGCTGGATTGGTATGGTGATGTTGATGGTACGCCGCTTTACCGATGATCGTATCTCGCATA
CCTCATCTTTTAGCGACAAATTGGTGCTGATACTGCTATTTATCCAGCTCAACTTAGGTCTGATATCTATCTTTACTTCC
ATGCATCATCTTGATGGGTTAACCATGATGAATCTTGCTGGCTGGGCACAAGATATTACGATCTTAAGACCTTGGCATGC
GGCCGCTCGTATCGAACAAGCCGATCTGATTTATCAGCTGCATATGGCGCTAGGCATTACCCTGATTATGATTTTCCCAT
TCACACGTTTGATTCATATCATCAGTGCGCCAGTGTGGTATTTGGGTCGCCGCTATCAGATCGTGAGACAAAAAACCTCT
CAGTAG

Upstream 100 bases:

>100_bases
AGACCGAAGTGTCATGCCGCCAAAACAAGTCGATGCCCCTGTGCATTGGGTGGATTTCGATACGTCGAAACCTGTTAAAC
AGCCTTAAGGAGAGAGTCTC

Downstream 100 bases:

>100_bases
GAATTTATCACAATTGCTATACAACAAAGGGTTGGTCCGGTAAAATCATAAGATTCATTTGAAATGAATCTTATGATAGA
ATAGCGCCCTGCAGTGATGA

Product: respiratory nitrate reductase subunit gamma

Products: NA

Alternate protein names: Cytochrome B-NR; Nitrate reductase A subunit gamma; Quinol-nitrate oxidoreductase subunit gamma [H]

Number of amino acids: Translated: 241; Mature: 241

Protein sequence:

>241_residues
MNIADPGVQSLANLNWLQIFLFGIYPYIALVVAILGTWARFDLSQYSWKTGSSQMLRSKGMRVASNLFHVGIIVVLLGHL
VGMLTPHFIYEPFISAAHKQILAVVVGGIAGAVCWIGMVMLMVRRFTDDRISHTSSFSDKLVLILLFIQLNLGLISIFTS
MHHLDGLTMMNLAGWAQDITILRPWHAAARIEQADLIYQLHMALGITLIMIFPFTRLIHIISAPVWYLGRRYQIVRQKTS
Q

Sequences:

>Translated_241_residues
MNIADPGVQSLANLNWLQIFLFGIYPYIALVVAILGTWARFDLSQYSWKTGSSQMLRSKGMRVASNLFHVGIIVVLLGHL
VGMLTPHFIYEPFISAAHKQILAVVVGGIAGAVCWIGMVMLMVRRFTDDRISHTSSFSDKLVLILLFIQLNLGLISIFTS
MHHLDGLTMMNLAGWAQDITILRPWHAAARIEQADLIYQLHMALGITLIMIFPFTRLIHIISAPVWYLGRRYQIVRQKTS
Q
>Mature_241_residues
MNIADPGVQSLANLNWLQIFLFGIYPYIALVVAILGTWARFDLSQYSWKTGSSQMLRSKGMRVASNLFHVGIIVVLLGHL
VGMLTPHFIYEPFISAAHKQILAVVVGGIAGAVCWIGMVMLMVRRFTDDRISHTSSFSDKLVLILLFIQLNLGLISIFTS
MHHLDGLTMMNLAGWAQDITILRPWHAAARIEQADLIYQLHMALGITLIMIFPFTRLIHIISAPVWYLGRRYQIVRQKTS
Q

Specific function: The nitrate reductase enzyme complex allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The gamma chain is a membrane-embedded heme-iron unit resembling cytochrome b, which transfers electrons from quinones to the beta subunit [

COG id: COG2181

COG function: function code C; Nitrate reductase gamma subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787480, Length=225, Percent_Identity=47.1111111111111, Blast_Score=217, Evalue=7e-58,
Organism=Escherichia coli, GI1787738, Length=225, Percent_Identity=45.3333333333333, Blast_Score=209, Evalue=1e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003816 [H]

Pfam domain/function: PF02665 Nitrate_red_gam [H]

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 27176; Mature: 27176

Theoretical pI: Translated: 10.21; Mature: 10.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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C
>Mature Secondary Structure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C

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2832376; 8905232; 9278503; 3053688; 12910261 [H]