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The map label for this gene is narH [H]

Identifier: 148652478

GI number: 148652478

Start: 811540

End: 813078

Strand: Direct

Name: narH [H]

Synonym: PsycPRwf_0667

Alternate gene names: 148652478

Gene position: 811540-813078 (Clockwise)

Preceding gene: 148652477

Following gene: 148652479

Centisome position: 27.24

GC content: 47.3

Gene sequence:

>1539_bases
ATGAAAATCAGATCACAAGTAGGCATGGTGCTTAACCTCGATAAATGTATCGGTTGTCACACCTGCTCAGTTACCTGTAA
AAACGTTTGGACCAGCCGTGAAGGTATGGAATACGCCTGGTTTAACAACGTAGAGTCAAAGCCGGGTATCGGTTATCCCA
AAGAATGGGAGAATCAAAATAAATGGAATGGCGGCTGGATTCGTAATGCCAATGGCACGATTAATCCACGCATCGGCGGT
AAATTTAGAGTGTTAGCGAACATCTTCGCTAACCCTGATTTGCCAGAGATTGATGACTATTATGAGCCGTTTGACTTTGA
TTATCAGCACTTGCACACCGCACCGATTAGCAATCATCAACCGATTGCGCGTCCTCGCTCTGCCATTACTGGTAAGCGGA
TGCAAAAAATCGAGTGGGGCCCTAACTGGGAGGAAATCTTAGGCTCAGAGTTTGAGAAGCGTCGCCGTGATAAAAACTTC
GATAACGTGCAAGCAGACATCTATGGCGAGTACGAAAACACCTTCATGATGTATCTGCCGCGTCTGTGTGAGCACTGCTT
AAACCCAACTTGCGTGGCGTCATGCCCAAGTGGGGCGATTTATAAGCGTGAAGAAGACGGTATTGTGTTGATTGACCAAG
ACAAATGCCGCGGCTGGCGTATGTGTATCTCAGGCTGCCCATACAAAAAGATTTACTACAACTGGAAATCGGGTAAATCT
GAAAAATGTATCTTCTGTTATCCACGTATCGAAGCGGGCATGCCAACCGTATGTTCTGAAACCTGTGTTGGCCGTATCCG
CTACCTTGGCGTGCTGTTATATGATGCTGATAAGATTGCGGAAGCTGCGAGCACCCCAAATGAGCAAGACTTATACCAAG
CACAGCTGGATGTATTCTTAGATCCAAACGATCCAGAAGTCATCGCTCAAGCATTAAAAGACGGCGTACCTCAATCTGTG
ATTGATGCGGCACAAAAATCACCGGTTTACAAACTGGCGATGGACTGGAAACTGGCACTGCCATTACACCCTGAGTATCG
TACGCTACCTATGGTGTGGTATGTACCGCCTTTATCACCGATTCAAAATGCAGCAGAAGCGGGCAAAGTGGGCATGGATG
GTCTGATTCCAGATGTCGACAGCTTACGTATTCCACTGCGTTATTTAGCCAACATGCTAACCGCTGGTGATGAAGAGCCG
GTACGCATGGCACTAAAACGCCTACTTGCCATGCGCAGCTACAAGCGTATGCAATTGGTTGAAAAGCAAGAAGTACAAAG
CATCCTAGATGATGTGGGCTTAACCAAATTGCAAGTCGAAGAGATGTACCGCTATCTAGCGATTGCCAACTATGAAGACC
GTTTTGTCATCCCAACGGCGCATAGAGAAGAGGCGATGAGTGATGCCTTCGCTGAGCGTAATGGTTGCGGCTTTACCTTT
GGTGAAGGCTGCTCAGGTAGCAGTGACACCAGCATGTTCGGTCAGAAAAAAGCAACCCGCCGTGACTTTATTGACACCGT
TCAAAAGTGGGAGGTGTAA

Upstream 100 bases:

>100_bases
TTGGCTAGAGGATAAGCCAGATGACTCATTACCACAGCCATTACCAACCACTTTAGAAGGTTAAGACAACCTTAAACACG
TTAATTTGGAGCACACACCC

Downstream 100 bases:

>100_bases
CATGACAGACGTTATCCAAAGCCAACGATTAGATGCCGGTATAGAGGACACGATGACCACTACACCAACCCTTGCAGACT
CGGATATGAAGGTTTTAAAA

Product: nitrate reductase subunit beta

Products: NA

Alternate protein names: Nitrate reductase A subunit beta; Quinol-nitrate oxidoreductase subunit beta [H]

Number of amino acids: Translated: 512; Mature: 512

Protein sequence:

>512_residues
MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGMEYAWFNNVESKPGIGYPKEWENQNKWNGGWIRNANGTINPRIGG
KFRVLANIFANPDLPEIDDYYEPFDFDYQHLHTAPISNHQPIARPRSAITGKRMQKIEWGPNWEEILGSEFEKRRRDKNF
DNVQADIYGEYENTFMMYLPRLCEHCLNPTCVASCPSGAIYKREEDGIVLIDQDKCRGWRMCISGCPYKKIYYNWKSGKS
EKCIFCYPRIEAGMPTVCSETCVGRIRYLGVLLYDADKIAEAASTPNEQDLYQAQLDVFLDPNDPEVIAQALKDGVPQSV
IDAAQKSPVYKLAMDWKLALPLHPEYRTLPMVWYVPPLSPIQNAAEAGKVGMDGLIPDVDSLRIPLRYLANMLTAGDEEP
VRMALKRLLAMRSYKRMQLVEKQEVQSILDDVGLTKLQVEEMYRYLAIANYEDRFVIPTAHREEAMSDAFAERNGCGFTF
GEGCSGSSDTSMFGQKKATRRDFIDTVQKWEV

Sequences:

>Translated_512_residues
MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGMEYAWFNNVESKPGIGYPKEWENQNKWNGGWIRNANGTINPRIGG
KFRVLANIFANPDLPEIDDYYEPFDFDYQHLHTAPISNHQPIARPRSAITGKRMQKIEWGPNWEEILGSEFEKRRRDKNF
DNVQADIYGEYENTFMMYLPRLCEHCLNPTCVASCPSGAIYKREEDGIVLIDQDKCRGWRMCISGCPYKKIYYNWKSGKS
EKCIFCYPRIEAGMPTVCSETCVGRIRYLGVLLYDADKIAEAASTPNEQDLYQAQLDVFLDPNDPEVIAQALKDGVPQSV
IDAAQKSPVYKLAMDWKLALPLHPEYRTLPMVWYVPPLSPIQNAAEAGKVGMDGLIPDVDSLRIPLRYLANMLTAGDEEP
VRMALKRLLAMRSYKRMQLVEKQEVQSILDDVGLTKLQVEEMYRYLAIANYEDRFVIPTAHREEAMSDAFAERNGCGFTF
GEGCSGSSDTSMFGQKKATRRDFIDTVQKWEV
>Mature_512_residues
MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGMEYAWFNNVESKPGIGYPKEWENQNKWNGGWIRNANGTINPRIGG
KFRVLANIFANPDLPEIDDYYEPFDFDYQHLHTAPISNHQPIARPRSAITGKRMQKIEWGPNWEEILGSEFEKRRRDKNF
DNVQADIYGEYENTFMMYLPRLCEHCLNPTCVASCPSGAIYKREEDGIVLIDQDKCRGWRMCISGCPYKKIYYNWKSGKS
EKCIFCYPRIEAGMPTVCSETCVGRIRYLGVLLYDADKIAEAASTPNEQDLYQAQLDVFLDPNDPEVIAQALKDGVPQSV
IDAAQKSPVYKLAMDWKLALPLHPEYRTLPMVWYVPPLSPIQNAAEAGKVGMDGLIPDVDSLRIPLRYLANMLTAGDEEP
VRMALKRLLAMRSYKRMQLVEKQEVQSILDDVGLTKLQVEEMYRYLAIANYEDRFVIPTAHREEAMSDAFAERNGCGFTF
GEGCSGSSDTSMFGQKKATRRDFIDTVQKWEV

Specific function: The nitrate reductase enzyme complex allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The beta chain is an electron transfer unit containing four cysteine clusters involved in the formation of iron-sulfur centers. Electrons ar

COG id: COG1140

COG function: function code C; Nitrate reductase beta subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787478, Length=512, Percent_Identity=74.609375, Blast_Score=813, Evalue=0.0,
Organism=Escherichia coli, GI1787740, Length=509, Percent_Identity=74.0667976424361, Blast_Score=807, Evalue=0.0,
Organism=Escherichia coli, GI1787872, Length=133, Percent_Identity=35.3383458646617, Blast_Score=89, Evalue=9e-19,
Organism=Escherichia coli, GI1787122, Length=133, Percent_Identity=35.3383458646617, Blast_Score=88, Evalue=2e-18,
Organism=Escherichia coli, GI2367345, Length=141, Percent_Identity=26.9503546099291, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI226510944, Length=124, Percent_Identity=30.6451612903226, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI1789370, Length=97, Percent_Identity=37.1134020618557, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1787749, Length=87, Percent_Identity=33.3333333333333, Blast_Score=62, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR006547 [H]

Pfam domain/function: NA

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 58466; Mature: 58466

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGMEYAWFNNVESKPGIGYPKEWENQN
CCCHHHCCCEEEHHHHHCCCEEEEEHHHHHCCCCCCEEHCCCCCCCCCCCCCCCCCCCCC
KWNGGWIRNANGTINPRIGGKFRVLANIFANPDLPEIDDYYEPFDFDYQHLHTAPISNHQ
CCCCCEEECCCCEECCCCCCHHEEHHHHHCCCCCCCCHHCCCCCCCCHHHEECCCCCCCC
PIARPRSAITGKRMQKIEWGPNWEEILGSEFEKRRRDKNFDNVQADIYGEYENTFMMYLP
CCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHH
RLCEHCLNPTCVASCPSGAIYKREEDGIVLIDQDKCRGWRMCISGCPYKKIYYNWKSGKS
HHHHHHCCCEEEECCCCCCCEEECCCCEEEEECCCCCCHHHHHCCCCCHHEEEEECCCCC
EKCIFCYPRIEAGMPTVCSETCVGRIRYLGVLLYDADKIAEAASTPNEQDLYQAQLDVFL
CCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHEECHHHHHHHHCCCCHHHHHHEEEEEEE
DPNDPEVIAQALKDGVPQSVIDAAQKSPVYKLAMDWKLALPLHPEYRTLPMVWYVPPLSP
CCCCHHHHHHHHHCCCCHHHHHHHHCCCCEEEEECEEEECCCCCCCCCCCEEEECCCCCH
IQNAAEAGKVGMDGLIPDVDSLRIPLRYLANMLTAGDEEPVRMALKRLLAMRSYKRMQLV
HHHHHHHCCCCCCCCCCCHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
EKQEVQSILDDVGLTKLQVEEMYRYLAIANYEDRFVIPTAHREEAMSDAFAERNGCGFTF
HHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHCCCCCCCC
GEGCSGSSDTSMFGQKKATRRDFIDTVQKWEV
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGMEYAWFNNVESKPGIGYPKEWENQN
CCCHHHCCCEEEHHHHHCCCEEEEEHHHHHCCCCCCEEHCCCCCCCCCCCCCCCCCCCCC
KWNGGWIRNANGTINPRIGGKFRVLANIFANPDLPEIDDYYEPFDFDYQHLHTAPISNHQ
CCCCCEEECCCCEECCCCCCHHEEHHHHHCCCCCCCCHHCCCCCCCCHHHEECCCCCCCC
PIARPRSAITGKRMQKIEWGPNWEEILGSEFEKRRRDKNFDNVQADIYGEYENTFMMYLP
CCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHH
RLCEHCLNPTCVASCPSGAIYKREEDGIVLIDQDKCRGWRMCISGCPYKKIYYNWKSGKS
HHHHHHCCCEEEECCCCCCCEEECCCCEEEEECCCCCCHHHHHCCCCCHHEEEEECCCCC
EKCIFCYPRIEAGMPTVCSETCVGRIRYLGVLLYDADKIAEAASTPNEQDLYQAQLDVFL
CCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHEECHHHHHHHHCCCCHHHHHHEEEEEEE
DPNDPEVIAQALKDGVPQSVIDAAQKSPVYKLAMDWKLALPLHPEYRTLPMVWYVPPLSP
CCCCHHHHHHHHHCCCCHHHHHHHHCCCCEEEEECEEEECCCCCCCCCCCEEEECCCCCH
IQNAAEAGKVGMDGLIPDVDSLRIPLRYLANMLTAGDEEPVRMALKRLLAMRSYKRMQLV
HHHHHHHCCCCCCCCCCCHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
EKQEVQSILDDVGLTKLQVEEMYRYLAIANYEDRFVIPTAHREEAMSDAFAERNGCGFTF
HHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHCCCCCCCC
GEGCSGSSDTSMFGQKKATRRDFIDTVQKWEV
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2674654; 8905232; 9278503; 2832376; 3053688; 1321049; 8383531; 8388253; 8664273; 9516445; 12910261 [H]