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The map label for this gene is narK [H]

Identifier: 148652477

GI number: 148652477

Start: 805820

End: 807202

Strand: Direct

Name: narK [H]

Synonym: PsycPRwf_0665

Alternate gene names: 148652477

Gene position: 805820-807202 (Clockwise)

Preceding gene: 148652474

Following gene: 148652478

Centisome position: 27.05

GC content: 48.45

Gene sequence:

>1383_bases
ATGGGTGTGAATAAAGAAGACTTAAAAGGGGGCAAGCTCATCACTGACTGGCGCCCAGAAATGGAAGATTTTTGGGAAAA
CGGTGGTAAGCAGGTCGCCAGCCGCAACTTATGGGTGTCTATCCCAGCACTGCTATTGGCCTTTGCGGTATGGATGGTCT
GGAGTGCGGTGATTGTTAACTTGCCTGAGATTGGCTTTGGCTTTGATGAGGGGCAGCTGTTTTGGCTGGCCGCTTTACCA
GGATTGTCTGGGGCGACGCTGCGTATTTTTTATTCGTTTATGGTGCCTATCTTTGGTGGACGTCGCTGGACGGCCATCTC
TACCGCGTCACTGCTTATCCCTGCAGTGTGGATGGGCTTTGCGGTTCAAAATCCTGATACGCCCTTTAGCGTGTTTGCCA
TGATTGCACTACTGTGCGGCTTTGGTGGCGCAAACTTTGCGTCCTCTATGTCCAACATCTCGTTTTTTTATCCTAAAGAT
AAGCAAGGCACAGCACTGGGTTTAAACGCAGGTCTGGGTAACCTAGGTGTGTCTGTGATGCAGTTTGTGGTGCCTTTGGT
AATCGGTTTTGGTGTGTTTGGCGCACTTGGCGGTGCACCGCAAGTCACTGAAGCGGGCAAAGCACTTTATCTGCAAAATG
CAGGCTTTATCTGGGTGCCTTTTATTGCACTGGTCTCTATTGCTGCTTGGTTTTTGATGAATGATATCGCCTCGGCAAAG
GCATCGTTTAAAGATCAAGCGGTTATCTTTAAGCGCAAGCACAACTGGATCATGTGTGTGCTATATATGGCCACCTTTGG
CTCGTTTATTGGCTTTTCGGCCGCCTTTCCGATGCTGATTAAAAAAGGATTTCCGGATGTCAATGCGTTGCAATTTGCCT
TTTTAGGCCCCTTAGTCGGTGCGTTATTCCGTCCTGCAGGTGGCTGGATTGCTGATAAATTGGGCGGCGCCCGAGTGACA
TTCTGGAACTATGTGGTGATGATATTGGCCGTAGTGGCGGTGATGTACTTCTTGCCAAGTGGCACCAGTGCCGGTAGCTT
TACGGGGTATCTGATCTCATTTATGGTACTGTTTGTCACCACAGGGATTGGTAATGGCTCAACCTTCCGTATGATTCCAG
TTATCTTTAGAACGGTATTAGAGCGTGAGCATCCTGACAAAGTAGGCACAGAGCCTTTGTTTGTTGAGATTCGCAAAGAG
TCAGCAGCAGTGGTGGGCTTTACCTCAGCAGTAGCGGCTTATGGTGCTTTCTTTGTGCCTAAGATGTTTGGCTCAGGCTT
AGGTGTCAATGGCACATTCATCGCTTTGATTGCCTATTATGTGGTTTGTATTGTCTTAACCTGGTGGTATTACAGCCGCA
AAAATGCAGAGTATCCGTCATAG

Upstream 100 bases:

>100_bases
ACCCTTATAACCATATTAAATTCATGATGGCTGAAGTACCTTAACTTATCGAACAACAAGCCGTGACTGTGAGTTATCAA
CAGTACTAAGTGAGGTAAGT

Downstream 100 bases:

>100_bases
GGCTATATTGTAGGGCTATAATGGTAAGACACACAATGTAGTTATTTAGGACTTACGCAGGATTAGGGATTTTAGCGATT
GGGTATTATTTTCTCGCTTG

Product: nitrite transporter

Products: NA

Alternate protein names: Nitrite facilitator 1 [H]

Number of amino acids: Translated: 460; Mature: 459

Protein sequence:

>460_residues
MGVNKEDLKGGKLITDWRPEMEDFWENGGKQVASRNLWVSIPALLLAFAVWMVWSAVIVNLPEIGFGFDEGQLFWLAALP
GLSGATLRIFYSFMVPIFGGRRWTAISTASLLIPAVWMGFAVQNPDTPFSVFAMIALLCGFGGANFASSMSNISFFYPKD
KQGTALGLNAGLGNLGVSVMQFVVPLVIGFGVFGALGGAPQVTEAGKALYLQNAGFIWVPFIALVSIAAWFLMNDIASAK
ASFKDQAVIFKRKHNWIMCVLYMATFGSFIGFSAAFPMLIKKGFPDVNALQFAFLGPLVGALFRPAGGWIADKLGGARVT
FWNYVVMILAVVAVMYFLPSGTSAGSFTGYLISFMVLFVTTGIGNGSTFRMIPVIFRTVLEREHPDKVGTEPLFVEIRKE
SAAVVGFTSAVAAYGAFFVPKMFGSGLGVNGTFIALIAYYVVCIVLTWWYYSRKNAEYPS

Sequences:

>Translated_460_residues
MGVNKEDLKGGKLITDWRPEMEDFWENGGKQVASRNLWVSIPALLLAFAVWMVWSAVIVNLPEIGFGFDEGQLFWLAALP
GLSGATLRIFYSFMVPIFGGRRWTAISTASLLIPAVWMGFAVQNPDTPFSVFAMIALLCGFGGANFASSMSNISFFYPKD
KQGTALGLNAGLGNLGVSVMQFVVPLVIGFGVFGALGGAPQVTEAGKALYLQNAGFIWVPFIALVSIAAWFLMNDIASAK
ASFKDQAVIFKRKHNWIMCVLYMATFGSFIGFSAAFPMLIKKGFPDVNALQFAFLGPLVGALFRPAGGWIADKLGGARVT
FWNYVVMILAVVAVMYFLPSGTSAGSFTGYLISFMVLFVTTGIGNGSTFRMIPVIFRTVLEREHPDKVGTEPLFVEIRKE
SAAVVGFTSAVAAYGAFFVPKMFGSGLGVNGTFIALIAYYVVCIVLTWWYYSRKNAEYPS
>Mature_459_residues
GVNKEDLKGGKLITDWRPEMEDFWENGGKQVASRNLWVSIPALLLAFAVWMVWSAVIVNLPEIGFGFDEGQLFWLAALPG
LSGATLRIFYSFMVPIFGGRRWTAISTASLLIPAVWMGFAVQNPDTPFSVFAMIALLCGFGGANFASSMSNISFFYPKDK
QGTALGLNAGLGNLGVSVMQFVVPLVIGFGVFGALGGAPQVTEAGKALYLQNAGFIWVPFIALVSIAAWFLMNDIASAKA
SFKDQAVIFKRKHNWIMCVLYMATFGSFIGFSAAFPMLIKKGFPDVNALQFAFLGPLVGALFRPAGGWIADKLGGARVTF
WNYVVMILAVVAVMYFLPSGTSAGSFTGYLISFMVLFVTTGIGNGSTFRMIPVIFRTVLEREHPDKVGTEPLFVEIRKES
AAVVGFTSAVAAYGAFFVPKMFGSGLGVNGTFIALIAYYVVCIVLTWWYYSRKNAEYPS

Specific function: Involved in excretion of nitrite produced by the dissimilatory reduction of nitrate [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

Organism=Escherichia coli, GI1787475, Length=452, Percent_Identity=55.0884955752212, Blast_Score=473, Evalue=1e-135,
Organism=Escherichia coli, GI1787743, Length=458, Percent_Identity=54.585152838428, Blast_Score=437, Evalue=1e-124,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004737 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 49967; Mature: 49836

Theoretical pI: Translated: 9.49; Mature: 9.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGVNKEDLKGGKLITDWRPEMEDFWENGGKQVASRNLWVSIPALLLAFAVWMVWSAVIVN
CCCCCCCCCCCCEEECCCCCHHHHHHCCCHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHC
LPEIGFGFDEGQLFWLAALPGLSGATLRIFYSFMVPIFGGRRWTAISTASLLIPAVWMGF
CCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHH
AVQNPDTPFSVFAMIALLCGFGGANFASSMSNISFFYPKDKQGTALGLNAGLGNLGVSVM
EECCCCCCHHHHHHHHHHHCCCCCHHHHHHCCCEEECCCCCCCCEEEECCCCHHHHHHHH
QFVVPLVIGFGVFGALGGAPQVTEAGKALYLQNAGFIWVPFIALVSIAAWFLMNDIASAK
HHHHHHHHHHHHHHHCCCCCCHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
ASFKDQAVIFKRKHNWIMCVLYMATFGSFIGFSAAFPMLIKKGFPDVNALQFAFLGPLVG
HCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
ALFRPAGGWIADKLGGARVTFWNYVVMILAVVAVMYFLPSGTSAGSFTGYLISFMVLFVT
HHHCCCCCHHHHHCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
TGIGNGSTFRMIPVIFRTVLEREHPDKVGTEPLFVEIRKESAAVVGFTSAVAAYGAFFVP
CCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCEEEHHHHHHHHHHHHHH
KMFGSGLGVNGTFIALIAYYVVCIVLTWWYYSRKNAEYPS
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
GVNKEDLKGGKLITDWRPEMEDFWENGGKQVASRNLWVSIPALLLAFAVWMVWSAVIVN
CCCCCCCCCCCEEECCCCCHHHHHHCCCHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHC
LPEIGFGFDEGQLFWLAALPGLSGATLRIFYSFMVPIFGGRRWTAISTASLLIPAVWMGF
CCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHH
AVQNPDTPFSVFAMIALLCGFGGANFASSMSNISFFYPKDKQGTALGLNAGLGNLGVSVM
EECCCCCCHHHHHHHHHHHCCCCCHHHHHHCCCEEECCCCCCCCEEEECCCCHHHHHHHH
QFVVPLVIGFGVFGALGGAPQVTEAGKALYLQNAGFIWVPFIALVSIAAWFLMNDIASAK
HHHHHHHHHHHHHHHCCCCCCHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
ASFKDQAVIFKRKHNWIMCVLYMATFGSFIGFSAAFPMLIKKGFPDVNALQFAFLGPLVG
HCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
ALFRPAGGWIADKLGGARVTFWNYVVMILAVVAVMYFLPSGTSAGSFTGYLISFMVLFVT
HHHCCCCCHHHHHCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
TGIGNGSTFRMIPVIFRTVLEREHPDKVGTEPLFVEIRKESAAVVGFTSAVAAYGAFFVP
CCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCEEEHHHHHHHHHHHHHH
KMFGSGLGVNGTFIALIAYYVVCIVLTWWYYSRKNAEYPS
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2668029; 8905232; 9278503; 2657652 [H]