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The map label for this gene is dapF

Identifier: 148652161

GI number: 148652161

Start: 421543

End: 422424

Strand: Direct

Name: dapF

Synonym: PsycPRwf_0349

Alternate gene names: 148652161

Gene position: 421543-422424 (Clockwise)

Preceding gene: 148652160

Following gene: 148652162

Centisome position: 14.15

GC content: 50.45

Gene sequence:

>882_bases
ATGCTTATAGAGTTTACAAAAATGCATGGGCTGGGCAATGACTTTATGGTCATTGACTTGGTCACTCAGCGTTTGGAGCT
GACACCAGAGCTGATTACATTATTGGCTGATCGTAACTTAGGTATTGGCTTCGATCAGCTGTTGGTGGTTGAGCCACCCA
TGCGTCCAGATGTTGATTTTCGCTACCGCATCTTTAATTCAGATGGTAGTGAAGTGGAGCAATGTGGTAATGGGGCACGC
TGTTTTGCCCGGTTTGTACAGGCGCGCAAACTGTCATTCAAGCAGCGTATTCGGGTTGAAACCAAGTCAGGTATTTTGAC
GTTATCGACCGATAATTACGGCTGGGTAGAGGTGGATATGGGCAAGCCGCGCTTTGAGCCGGCAGAGATCCCTTTTACGC
CCAAAGCCATTACTAAAATACAAAACGCTTATCACCTAGATGTCGAGGGGACGCCGGTACAATTGTATGTGGCCAATATG
GGCAACCCACATGCGGTTATTAAGGTCGATGATATCTTGACGGCCGATGTTGAGAAGCTTGGCAAAGCCATTGAGTCGCA
TCCGGCATTTCCTGAGCGGGTCAATGTTGGCTTTATGCAGGTGATGAACCAGCGTCACATTCGTCTGCGTGTCTATGAGC
GCGGTGTGGGCGAGACCCAAGCTTGTGGTACAGGTGCTTGTGCGGCGGTCGCTACAGGCATTCGCGAAGGCTGGCTGGAT
GAGGGCGAGGAGATTAGAGCGCAGCTTTATGGCGGCAGTTTACTGATTCGCTGGTCACCTGGCTATTCTGTGATGATGAC
CGGCCCGACCGCTTTCGTCTATGAAGGTGTGTTTAGTCCTGATGGTATTATGGCGCAGGCCGGTCTTAAGCCATTAAGCT
AG

Upstream 100 bases:

>100_bases
GGCAGATAAGCCAATTGCCAATACAGCACAGTTAGGCATTATCATTGTGATATTGTGCATGTTAAGATAAGCCATAAATA
GAATCTAAGGAATATAAAAT

Downstream 100 bases:

>100_bases
GTGTAAGCAAGGTTAGGTCAACCACAGTCATGACAAATAAAGTGCTGGAGAAGCAGCAGCTAGAGAATAAGAAGCAGCTA
GAGGATTCTTTGGCCACCAA

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MLIEFTKMHGLGNDFMVIDLVTQRLELTPELITLLADRNLGIGFDQLLVVEPPMRPDVDFRYRIFNSDGSEVEQCGNGAR
CFARFVQARKLSFKQRIRVETKSGILTLSTDNYGWVEVDMGKPRFEPAEIPFTPKAITKIQNAYHLDVEGTPVQLYVANM
GNPHAVIKVDDILTADVEKLGKAIESHPAFPERVNVGFMQVMNQRHIRLRVYERGVGETQACGTGACAAVATGIREGWLD
EGEEIRAQLYGGSLLIRWSPGYSVMMTGPTAFVYEGVFSPDGIMAQAGLKPLS

Sequences:

>Translated_293_residues
MLIEFTKMHGLGNDFMVIDLVTQRLELTPELITLLADRNLGIGFDQLLVVEPPMRPDVDFRYRIFNSDGSEVEQCGNGAR
CFARFVQARKLSFKQRIRVETKSGILTLSTDNYGWVEVDMGKPRFEPAEIPFTPKAITKIQNAYHLDVEGTPVQLYVANM
GNPHAVIKVDDILTADVEKLGKAIESHPAFPERVNVGFMQVMNQRHIRLRVYERGVGETQACGTGACAAVATGIREGWLD
EGEEIRAQLYGGSLLIRWSPGYSVMMTGPTAFVYEGVFSPDGIMAQAGLKPLS
>Mature_293_residues
MLIEFTKMHGLGNDFMVIDLVTQRLELTPELITLLADRNLGIGFDQLLVVEPPMRPDVDFRYRIFNSDGSEVEQCGNGAR
CFARFVQARKLSFKQRIRVETKSGILTLSTDNYGWVEVDMGKPRFEPAEIPFTPKAITKIQNAYHLDVEGTPVQLYVANM
GNPHAVIKVDDILTADVEKLGKAIESHPAFPERVNVGFMQVMNQRHIRLRVYERGVGETQACGTGACAAVATGIREGWLD
EGEEIRAQLYGGSLLIRWSPGYSVMMTGPTAFVYEGVFSPDGIMAQAGLKPLS

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family

Homologues:

Organism=Escherichia coli, GI87082334, Length=275, Percent_Identity=57.8181818181818, Blast_Score=324, Evalue=3e-90,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPF_PSYWF (A5WCB3)

Other databases:

- EMBL:   CP000713
- RefSeq:   YP_001279254.1
- ProteinModelPortal:   A5WCB3
- SMR:   A5WCB3
- STRING:   A5WCB3
- GeneID:   5205333
- GenomeReviews:   CP000713_GR
- KEGG:   prw:PsycPRwf_0349
- eggNOG:   COG0253
- HOGENOM:   HBG399442
- OMA:   HTGIGFD
- ProtClustDB:   PRK00450
- BioCyc:   PSP56811:PSYCPRWF_0349-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00197
- InterPro:   IPR001653
- InterPro:   IPR018510
- TIGRFAMs:   TIGR00652

Pfam domain/function: PF01678 DAP_epimerase

EC number: =5.1.1.7

Molecular weight: Translated: 32542; Mature: 32542

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: ACT_SITE 75-75 ACT_SITE 222-222

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIEFTKMHGLGNDFMVIDLVTQRLELTPELITLLADRNLGIGFDQLLVVEPPMRPDVDF
CEEEEEECCCCCCCEEEEEEHHHHHHCCHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCE
RYRIFNSDGSEVEQCGNGARCFARFVQARKLSFKQRIRVETKSGILTLSTDNYGWVEVDM
EEEEECCCCHHHHHHCCHHHHHHHHHHHHHHCHHHHEEEEECCCEEEEECCCCCEEEEEC
GKPRFEPAEIPFTPKAITKIQNAYHLDVEGTPVQLYVANMGNPHAVIKVDDILTADVEKL
CCCCCCCCCCCCCHHHHHHHCCEEEEECCCCEEEEEEECCCCCEEEEEECCHHHHHHHHH
GKAIESHPAFPERVNVGFMQVMNQRHIRLRVYERGVGETQACGTGACAAVATGIREGWLD
HHHHHHCCCCCHHCCHHHHHHHCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCC
EGEEIRAQLYGGSLLIRWSPGYSVMMTGPTAFVYEGVFSPDGIMAQAGLKPLS
CCHHEEEEEECCEEEEEECCCCEEEEECCCEEEEECCCCCCCEEHHCCCCCCC
>Mature Secondary Structure
MLIEFTKMHGLGNDFMVIDLVTQRLELTPELITLLADRNLGIGFDQLLVVEPPMRPDVDF
CEEEEEECCCCCCCEEEEEEHHHHHHCCHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCE
RYRIFNSDGSEVEQCGNGARCFARFVQARKLSFKQRIRVETKSGILTLSTDNYGWVEVDM
EEEEECCCCHHHHHHCCHHHHHHHHHHHHHHCHHHHEEEEECCCEEEEECCCCCEEEEEC
GKPRFEPAEIPFTPKAITKIQNAYHLDVEGTPVQLYVANMGNPHAVIKVDDILTADVEKL
CCCCCCCCCCCCCHHHHHHHCCEEEEECCCCEEEEEEECCCCCEEEEEECCHHHHHHHHH
GKAIESHPAFPERVNVGFMQVMNQRHIRLRVYERGVGETQACGTGACAAVATGIREGWLD
HHHHHHCCCCCHHCCHHHHHHHCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCC
EGEEIRAQLYGGSLLIRWSPGYSVMMTGPTAFVYEGVFSPDGIMAQAGLKPLS
CCHHEEEEEECCEEEEEECCCCEEEEECCCEEEEECCCCCCCEEHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA