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The map label for this gene is nemA [H]

Identifier: 148652153

GI number: 148652153

Start: 411692

End: 412792

Strand: Direct

Name: nemA [H]

Synonym: PsycPRwf_0341

Alternate gene names: 148652153

Gene position: 411692-412792 (Clockwise)

Preceding gene: 148652152

Following gene: 148652159

Centisome position: 13.82

GC content: 49.5

Gene sequence:

>1101_bases
ATGGCATACGCCCCGTTATTTAACCCCATTAAAATGGGCAACCAAACCTTTAAAAACCGTGTATTTATGGCGCCTTTGAC
CCGTCTGCGCTCTATTGAGCCCAGTGATATGCCCACCATATTAGCCGCTGAATACTACTCACAGCGTGCAGGCTCAGGTC
TGATTATTACAGAGGCCACTCAAGTGTCGTTGCAGGCCAAAGGCTACGCAGGTGCACCAGGTCTGCATAACGAGGAGCAG
GTAGCAGCATGGAAAGTCATTACCGATGCTGTACATGCCAAAGGCGGCAAAATTGTGGTGCAGCTGTGGCACACCGGCCT
GGTATCACACAACAGCGTACAGCCTGATAATAAGGCGCCTATTTCAGCCTCTGATGTTGATGTTGGTGTCCGTACTACCT
TACGTGATGACCAAGGTGCTCCTATCCGTGTTGAGGCCACAACGCCTCGTATGGCAAGCTTAGAAGAGATTAAACAAGTC
GTCGCAGATTTCGCTCAAGCGACCCAATATGCCAAGCAAGCCGGCTTTGATGGTATAGAGATCCACGGTGCCCACGGTTA
TTTATTACATCAATTCTGGGCGGAGCATACCAACAAGCGTACAGACAACTATGGTGGTAGCCGTGAAAACCGCGCCCGCT
TAATGCTTGAGGTTATCGATGCTTGTGTGGCTGCTTGGGACCAAGATCATGTGGGCATTCGCATCTCGCCACTGGGCAGC
TTTAACAACGTTGATGTGGGTTATAACGAAGATGAGGCGATTTGGTTGGTTGAGCAAATCAACAGCCGCAACCTTATGTA
TTTGCATATGTCTGAGCCAGACTGGGCCGGTGGCACGCCTTACTCAGAAGCGTTTAGACAAAAACTACGGGCGGCTTTTA
ACAATCCTATCGTGGCCGCTGGTGGCTATAGCGCCCAAAAAGCGCAGGATTTAATTGAAGCGGGCTATATTGATGCGGTG
GCCTTTGGTCGTGATTATATCGCCAACCCAGATTTAGCAGAGCGTCTCCAAAAAGATGCCGCGTTAAACGAGCAGCATCC
TCAAACCTTCTATGGTGGTGGTCTACAAGGCTATATTGACTATCCATTTATGAGCCAATAA

Upstream 100 bases:

>100_bases
AACTATGAGCCATTATGGCGCTAAATTATCCAACTCATAAACCGATAGGTTTAGTGCAGTAAGACAGTGAAACTTTATGA
ATAACTAAAAAGGAAGTCGC

Downstream 100 bases:

>100_bases
GCGTGTCATAGACGCGCTCTTAATAGCGGTCTATATTATTAAATAACAGCGCACATAAAAATCCCCAGTCTAGGCTTATA
GGCTGGGGATTTTTTTGGCT

Product: N-ethylmaleimide reductase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 366; Mature: 365

Protein sequence:

>366_residues
MAYAPLFNPIKMGNQTFKNRVFMAPLTRLRSIEPSDMPTILAAEYYSQRAGSGLIITEATQVSLQAKGYAGAPGLHNEEQ
VAAWKVITDAVHAKGGKIVVQLWHTGLVSHNSVQPDNKAPISASDVDVGVRTTLRDDQGAPIRVEATTPRMASLEEIKQV
VADFAQATQYAKQAGFDGIEIHGAHGYLLHQFWAEHTNKRTDNYGGSRENRARLMLEVIDACVAAWDQDHVGIRISPLGS
FNNVDVGYNEDEAIWLVEQINSRNLMYLHMSEPDWAGGTPYSEAFRQKLRAAFNNPIVAAGGYSAQKAQDLIEAGYIDAV
AFGRDYIANPDLAERLQKDAALNEQHPQTFYGGGLQGYIDYPFMSQ

Sequences:

>Translated_366_residues
MAYAPLFNPIKMGNQTFKNRVFMAPLTRLRSIEPSDMPTILAAEYYSQRAGSGLIITEATQVSLQAKGYAGAPGLHNEEQ
VAAWKVITDAVHAKGGKIVVQLWHTGLVSHNSVQPDNKAPISASDVDVGVRTTLRDDQGAPIRVEATTPRMASLEEIKQV
VADFAQATQYAKQAGFDGIEIHGAHGYLLHQFWAEHTNKRTDNYGGSRENRARLMLEVIDACVAAWDQDHVGIRISPLGS
FNNVDVGYNEDEAIWLVEQINSRNLMYLHMSEPDWAGGTPYSEAFRQKLRAAFNNPIVAAGGYSAQKAQDLIEAGYIDAV
AFGRDYIANPDLAERLQKDAALNEQHPQTFYGGGLQGYIDYPFMSQ
>Mature_365_residues
AYAPLFNPIKMGNQTFKNRVFMAPLTRLRSIEPSDMPTILAAEYYSQRAGSGLIITEATQVSLQAKGYAGAPGLHNEEQV
AAWKVITDAVHAKGGKIVVQLWHTGLVSHNSVQPDNKAPISASDVDVGVRTTLRDDQGAPIRVEATTPRMASLEEIKQVV
ADFAQATQYAKQAGFDGIEIHGAHGYLLHQFWAEHTNKRTDNYGGSRENRARLMLEVIDACVAAWDQDHVGIRISPLGSF
NNVDVGYNEDEAIWLVEQINSRNLMYLHMSEPDWAGGTPYSEAFRQKLRAAFNNPIVAAGGYSAQKAQDLIEAGYIDAVA
FGRDYIANPDLAERLQKDAALNEQHPQTFYGGGLQGYIDYPFMSQ

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=365, Percent_Identity=59.7260273972603, Blast_Score=469, Evalue=1e-134,
Organism=Escherichia coli, GI1789463, Length=355, Percent_Identity=31.5492957746479, Blast_Score=136, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17565138, Length=364, Percent_Identity=33.2417582417582, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI17559802, Length=358, Percent_Identity=31.0055865921788, Blast_Score=118, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI72001454, Length=354, Percent_Identity=30.225988700565, Blast_Score=111, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI17559804, Length=358, Percent_Identity=30.1675977653631, Blast_Score=110, Evalue=9e-25,
Organism=Caenorhabditis elegans, GI17564188, Length=353, Percent_Identity=28.8951841359773, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17540738, Length=269, Percent_Identity=28.2527881040892, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17566914, Length=282, Percent_Identity=28.3687943262411, Blast_Score=76, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6325086, Length=377, Percent_Identity=36.3395225464191, Blast_Score=216, Evalue=4e-57,
Organism=Saccharomyces cerevisiae, GI6321973, Length=379, Percent_Identity=36.9393139841689, Blast_Score=211, Evalue=1e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 40314; Mature: 40182

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYAPLFNPIKMGNQTFKNRVFMAPLTRLRSIEPSDMPTILAAEYYSQRAGSGLIITEAT
CCCCCCCCHHHHCCHHHHCEEEHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCEEEEEECE
QVSLQAKGYAGAPGLHNEEQVAAWKVITDAVHAKGGKIVVQLWHTGLVSHNSVQPDNKAP
EEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCC
ISASDVDVGVRTTLRDDQGAPIRVEATTPRMASLEEIKQVVADFAQATQYAKQAGFDGIE
CCCCCCCCCEEEEECCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
IHGAHGYLLHQFWAEHTNKRTDNYGGSRENRARLMLEVIDACVAAWDQDHVGIRISPLGS
EECCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCC
FNNVDVGYNEDEAIWLVEQINSRNLMYLHMSEPDWAGGTPYSEAFRQKLRAAFNNPIVAA
CCCCCCCCCCCCEEEEEEECCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCEEEE
GGYSAQKAQDLIEAGYIDAVAFGRDYIANPDLAERLQKDAALNEQHPQTFYGGGLQGYID
CCCCHHHHHHHHHHCCHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCEECCCCCEEEC
YPFMSQ
CCCCCC
>Mature Secondary Structure 
AYAPLFNPIKMGNQTFKNRVFMAPLTRLRSIEPSDMPTILAAEYYSQRAGSGLIITEAT
CCCCCCCHHHHCCHHHHCEEEHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCEEEEEECE
QVSLQAKGYAGAPGLHNEEQVAAWKVITDAVHAKGGKIVVQLWHTGLVSHNSVQPDNKAP
EEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCC
ISASDVDVGVRTTLRDDQGAPIRVEATTPRMASLEEIKQVVADFAQATQYAKQAGFDGIE
CCCCCCCCCEEEEECCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
IHGAHGYLLHQFWAEHTNKRTDNYGGSRENRARLMLEVIDACVAAWDQDHVGIRISPLGS
EECCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCC
FNNVDVGYNEDEAIWLVEQINSRNLMYLHMSEPDWAGGTPYSEAFRQKLRAAFNNPIVAA
CCCCCCCCCCCCEEEEEEECCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCEEEE
GGYSAQKAQDLIEAGYIDAVAFGRDYIANPDLAERLQKDAALNEQHPQTFYGGGLQGYID
CCCCHHHHHHHHHHCCHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCEECCCCCEEEC
YPFMSQ
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]