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The map label for this gene is lpdG [H]

Identifier: 148652080

GI number: 148652080

Start: 317980

End: 319437

Strand: Direct

Name: lpdG [H]

Synonym: PsycPRwf_0268

Alternate gene names: 148652080

Gene position: 317980-319437 (Clockwise)

Preceding gene: 148652079

Following gene: 148652081

Centisome position: 10.67

GC content: 46.78

Gene sequence:

>1458_bases
ATGAAAGACAATTATGATTTAGTGGTTATTGGCGGTGGTCCAGGTGGTTATGAAGCCGCTATTCGTGCCGGTCAATTGGG
TATGAGCGTAGCCTGTATCGAAAAACGCGTTTATAAAGGTGAGCCTGCATTGGGCGGTACTTGCTTAAACGTCGGTTGTA
TCCCATCAAAAGCTTTACTAGACTCTTCACACCGTTATGAAGCCACCAAACACGAGCTTGCAGAGCATGGTATCACTACC
AGCGATGTTGAGATTGACGTGGCCAAAATGATCGAGCGTAAAGAAGGCATCGTTAAGCAGTTAACCGGCGGTGTTGCTAT
GCTACTTAAAGGCAACGGCGTAGACTGGTTACAAGGCTGGGGTACGCTAGTTGACGGTAAAGGCAGCGACAAAAAAGTTA
AATTTACGGCGCTAGCAGATGAGTCTGAGACTGTTATCACCGCTAAAAACGTTATTTTAGCAGCCGGTTCTGTGCCTATT
GATATCCCTGTTGCGCCAGTCAACAACGAAGAAGGCATTATCGTTGACTCAACAGGTGCGCTAGATTTCACTGAAACACC
TAAGCGTCTAGGCGTGATTGGTGCTGGTGTTATCGGTCTTGAGCTAGGCTCTGTATGGCGTCGTCTTGGCGCTGAAGTGG
TGGTATATGAAGCGCTACCTGAATTCTTAGCAGCAGCTGACAAAGACATTGCTAAAGAAGCGGGCAAATTGCTTAAAAAA
CAAGGCCTAGATATCCGCGTTGATACCAAAGTAACCGGCACTGAAATAAAAGATGGTCAAGTGGTTGTGACCAGCGAGAA
AGGCGGTGAGTCTGCTGAGGAAACCTTCGACAAGCTAATCGTTTGTGTGGGTCGCCGTGCTTACTCTGAAAAATTACTGG
GTGAAGATTCAGGCATCACGCTAACTGACCGCGGTTTGGTTGAAGTTGATGATCAGTGCAAAACCAACCTAGATGGCGTA
TATGCTATCGGTGACTTGGTACGTGGCCCAATGCTTGCGCACAAAGCGATGGAAGAGGGCATGATGGCCGTTGAACGTAT
CCATGGCGAAAAAGCACAAGTGAACTATGATACTATTATCAACGTTATCTATACCCATCCAGAAATCGCTTGGGTAGGCT
TAACTGAGCAGCAAGCGGTAGAGCAAGGTTATGAGGTAAAAACCGGTTCATTTAACCTAGCAGCTAACGGTCGTGCTTTA
GCACAAAGCGAAGCTCAAGGTAGCATTAAAGTAGTGGCTGACGCTAAGACTGACCGCTTATTGGGTATGCATGCTATTTG
TGCTGGTGCCGGCGATATCGTTCACCAAGGTATGATTGCGATGGAATTTGTTTCAAGCATTGAAGACCTACAGTTAATGA
CCTTCGCTCACCCAACCATCAGTGAAGCGGTACACGAAGCTGCTTTATCTGCTGATGGCCGTGCTATCCATGCGATTCAG
CGTAAAAAACGTAAATAA

Upstream 100 bases:

>100_bases
TCGCTAAAGACTTAATAAGCATAGCTAAGTAGGTCATTTCATAATTCAAACTATAAATAAAGAGCTTTTACAATTCTTTG
ATTAAAAGACGGAATATACT

Downstream 100 bases:

>100_bases
CAAAAACAGTTATAGACACCAATAAATCACTCATAGGCGGTTAATAGTCTATGAGTGATTTGACAAAGTCTGTTACTAAA
GTGAGTTGCTGAGTGTAATT

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: E3 component of 2-oxoglutarate dehydrogenase complex; Glycine oxidation system L-factor; LPD-GLC [H]

Number of amino acids: Translated: 485; Mature: 485

Protein sequence:

>485_residues
MKDNYDLVVIGGGPGGYEAAIRAGQLGMSVACIEKRVYKGEPALGGTCLNVGCIPSKALLDSSHRYEATKHELAEHGITT
SDVEIDVAKMIERKEGIVKQLTGGVAMLLKGNGVDWLQGWGTLVDGKGSDKKVKFTALADESETVITAKNVILAAGSVPI
DIPVAPVNNEEGIIVDSTGALDFTETPKRLGVIGAGVIGLELGSVWRRLGAEVVVYEALPEFLAAADKDIAKEAGKLLKK
QGLDIRVDTKVTGTEIKDGQVVVTSEKGGESAEETFDKLIVCVGRRAYSEKLLGEDSGITLTDRGLVEVDDQCKTNLDGV
YAIGDLVRGPMLAHKAMEEGMMAVERIHGEKAQVNYDTIINVIYTHPEIAWVGLTEQQAVEQGYEVKTGSFNLAANGRAL
AQSEAQGSIKVVADAKTDRLLGMHAICAGAGDIVHQGMIAMEFVSSIEDLQLMTFAHPTISEAVHEAALSADGRAIHAIQ
RKKRK

Sequences:

>Translated_485_residues
MKDNYDLVVIGGGPGGYEAAIRAGQLGMSVACIEKRVYKGEPALGGTCLNVGCIPSKALLDSSHRYEATKHELAEHGITT
SDVEIDVAKMIERKEGIVKQLTGGVAMLLKGNGVDWLQGWGTLVDGKGSDKKVKFTALADESETVITAKNVILAAGSVPI
DIPVAPVNNEEGIIVDSTGALDFTETPKRLGVIGAGVIGLELGSVWRRLGAEVVVYEALPEFLAAADKDIAKEAGKLLKK
QGLDIRVDTKVTGTEIKDGQVVVTSEKGGESAEETFDKLIVCVGRRAYSEKLLGEDSGITLTDRGLVEVDDQCKTNLDGV
YAIGDLVRGPMLAHKAMEEGMMAVERIHGEKAQVNYDTIINVIYTHPEIAWVGLTEQQAVEQGYEVKTGSFNLAANGRAL
AQSEAQGSIKVVADAKTDRLLGMHAICAGAGDIVHQGMIAMEFVSSIEDLQLMTFAHPTISEAVHEAALSADGRAIHAIQ
RKKRK
>Mature_485_residues
MKDNYDLVVIGGGPGGYEAAIRAGQLGMSVACIEKRVYKGEPALGGTCLNVGCIPSKALLDSSHRYEATKHELAEHGITT
SDVEIDVAKMIERKEGIVKQLTGGVAMLLKGNGVDWLQGWGTLVDGKGSDKKVKFTALADESETVITAKNVILAAGSVPI
DIPVAPVNNEEGIIVDSTGALDFTETPKRLGVIGAGVIGLELGSVWRRLGAEVVVYEALPEFLAAADKDIAKEAGKLLKK
QGLDIRVDTKVTGTEIKDGQVVVTSEKGGESAEETFDKLIVCVGRRAYSEKLLGEDSGITLTDRGLVEVDDQCKTNLDGV
YAIGDLVRGPMLAHKAMEEGMMAVERIHGEKAQVNYDTIINVIYTHPEIAWVGLTEQQAVEQGYEVKTGSFNLAANGRAL
AQSEAQGSIKVVADAKTDRLLGMHAICAGAGDIVHQGMIAMEFVSSIEDLQLMTFAHPTISEAVHEAALSADGRAIHAIQ
RKKRK

Specific function: Also acts in the glycine cleavage system [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=478, Percent_Identity=45.1882845188285, Blast_Score=383, Evalue=1e-106,
Organism=Homo sapiens, GI50301238, Length=478, Percent_Identity=26.3598326359833, Blast_Score=138, Evalue=1e-32,
Organism=Homo sapiens, GI22035672, Length=482, Percent_Identity=27.5933609958506, Blast_Score=105, Evalue=7e-23,
Organism=Homo sapiens, GI148277071, Length=480, Percent_Identity=25, Blast_Score=102, Evalue=9e-22,
Organism=Homo sapiens, GI148277065, Length=480, Percent_Identity=25, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI33519430, Length=480, Percent_Identity=25, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI33519428, Length=480, Percent_Identity=25, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI33519426, Length=480, Percent_Identity=25, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI291045266, Length=488, Percent_Identity=24.7950819672131, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI291045268, Length=377, Percent_Identity=25.7294429708223, Blast_Score=86, Evalue=9e-17,
Organism=Escherichia coli, GI1786307, Length=480, Percent_Identity=39.1666666666667, Blast_Score=313, Evalue=2e-86,
Organism=Escherichia coli, GI87081717, Length=467, Percent_Identity=26.9807280513919, Blast_Score=174, Evalue=1e-44,
Organism=Escherichia coli, GI1789915, Length=450, Percent_Identity=28.2222222222222, Blast_Score=155, Evalue=4e-39,
Organism=Escherichia coli, GI87082354, Length=479, Percent_Identity=29.2275574112735, Blast_Score=155, Evalue=5e-39,
Organism=Caenorhabditis elegans, GI32565766, Length=478, Percent_Identity=43.5146443514644, Blast_Score=377, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI17557007, Length=476, Percent_Identity=26.0504201680672, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI71983429, Length=446, Percent_Identity=26.0089686098655, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI71983419, Length=446, Percent_Identity=26.0089686098655, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI71982272, Length=493, Percent_Identity=25.5578093306288, Blast_Score=100, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6321091, Length=487, Percent_Identity=42.5051334702259, Blast_Score=365, Evalue=1e-101,
Organism=Saccharomyces cerevisiae, GI6325240, Length=479, Percent_Identity=29.4363256784969, Blast_Score=183, Evalue=7e-47,
Organism=Saccharomyces cerevisiae, GI6325166, Length=478, Percent_Identity=25.7322175732218, Blast_Score=133, Evalue=6e-32,
Organism=Drosophila melanogaster, GI21358499, Length=470, Percent_Identity=45.1063829787234, Blast_Score=381, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24640549, Length=490, Percent_Identity=29.3877551020408, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI24640553, Length=490, Percent_Identity=29.3877551020408, Blast_Score=159, Evalue=4e-39,
Organism=Drosophila melanogaster, GI24640551, Length=490, Percent_Identity=29.3877551020408, Blast_Score=159, Evalue=5e-39,
Organism=Drosophila melanogaster, GI17737741, Length=484, Percent_Identity=26.0330578512397, Blast_Score=130, Evalue=2e-30,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 51599; Mature: 51599

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDNYDLVVIGGGPGGYEAAIRAGQLGMSVACIEKRVYKGEPALGGTCLNVGCIPSKALL
CCCCCEEEEEECCCCCHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHH
DSSHRYEATKHELAEHGITTSDVEIDVAKMIERKEGIVKQLTGGVAMLLKGNGVDWLQGW
CCCCCCHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHCC
GTLVDGKGSDKKVKFTALADESETVITAKNVILAAGSVPIDIPVAPVNNEEGIIVDSTGA
CEEECCCCCCCEEEEEEECCCCCEEEEECCEEEEECCCCEEEEEECCCCCCCEEEECCCC
LDFTETPKRLGVIGAGVIGLELGSVWRRLGAEVVVYEALPEFLAAADKDIAKEAGKLLKK
CCCCCCCHHHCEEECCHHHHHHHHHHHHHCCCEEEHHHHHHHHHHCCHHHHHHHHHHHHH
QGLDIRVDTKVTGTEIKDGQVVVTSEKGGESAEETFDKLIVCVGRRAYSEKLLGEDSGIT
CCCCEEEECEECCCEECCCEEEEEECCCCCHHHHHHHHHHHHHCCHHHHHHHCCCCCCEE
LTDRGLVEVDDQCKTNLDGVYAIGDLVRGPMLAHKAMEEGMMAVERIHGEKAQVNYDTII
EECCCCEEECHHHHCCCCCEEEHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCEECHHHEE
NVIYTHPEIAWVGLTEQQAVEQGYEVKTGSFNLAANGRALAQSEAQGSIKVVADAKTDRL
EEEEECCCEEEEECCHHHHHHCCCEEECCCEEEEECCCHHHHCCCCCCEEEEEECCCCHH
LGMHAICAGAGDIVHQGMIAMEFVSSIEDLQLMTFAHPTISEAVHEAALSADGRAIHAIQ
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHCCCCCCHHHHH
RKKRK
HHHCC
>Mature Secondary Structure
MKDNYDLVVIGGGPGGYEAAIRAGQLGMSVACIEKRVYKGEPALGGTCLNVGCIPSKALL
CCCCCEEEEEECCCCCHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHH
DSSHRYEATKHELAEHGITTSDVEIDVAKMIERKEGIVKQLTGGVAMLLKGNGVDWLQGW
CCCCCCHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHCC
GTLVDGKGSDKKVKFTALADESETVITAKNVILAAGSVPIDIPVAPVNNEEGIIVDSTGA
CEEECCCCCCCEEEEEEECCCCCEEEEECCEEEEECCCCEEEEEECCCCCCCEEEECCCC
LDFTETPKRLGVIGAGVIGLELGSVWRRLGAEVVVYEALPEFLAAADKDIAKEAGKLLKK
CCCCCCCHHHCEEECCHHHHHHHHHHHHHCCCEEEHHHHHHHHHHCCHHHHHHHHHHHHH
QGLDIRVDTKVTGTEIKDGQVVVTSEKGGESAEETFDKLIVCVGRRAYSEKLLGEDSGIT
CCCCEEEECEECCCEECCCEEEEEECCCCCHHHHHHHHHHHHHCCHHHHHHHCCCCCCEE
LTDRGLVEVDDQCKTNLDGVYAIGDLVRGPMLAHKAMEEGMMAVERIHGEKAQVNYDTII
EECCCCEEECHHHHCCCCCEEEHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCEECHHHEE
NVIYTHPEIAWVGLTEQQAVEQGYEVKTGSFNLAANGRALAQSEAQGSIKVVADAKTDRL
EEEEECCCEEEEECCHHHHHHCCCEEECCCEEEEECCCHHHHCCCCCCEEEEEECCCCHH
LGMHAICAGAGDIVHQGMIAMEFVSSIEDLQLMTFAHPTISEAVHEAALSADGRAIHAIQ
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHCCCCCCHHHHH
RKKRK
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]