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The map label for this gene is sucB [H]

Identifier: 148652079

GI number: 148652079

Start: 316529

End: 317758

Strand: Direct

Name: sucB [H]

Synonym: PsycPRwf_0267

Alternate gene names: 148652079

Gene position: 316529-317758 (Clockwise)

Preceding gene: 148652078

Following gene: 148652080

Centisome position: 10.63

GC content: 47.64

Gene sequence:

>1230_bases
ATGGCAGAAATTAAAGCGCCCGTATTCCCAGAGTCAGTTGCTGACGGTACCATCGTAGAATGGCACGTAAGCGAAGGCGA
ACAAGTTAACCGTGATGACATTTTAGCTGAAATCGAAACAGATAAAGTGGTCTTAGAAGTGGTTGCTCCAGATGACGGTG
TGGTAACTAAAATTGTTAAGCAAGTAGACGACACTGTGTTGTCTGACGAGCTAATCGCTGAATTTGAAGCTGGTGCAACA
GGCAGCGCTGCTGGTGCTTCTGAAGACAAAGCAGAAGAAAGCAGTGACACGGCTACTGAAGAAAAACCAGCAGCTAAGCC
TGCCGCAAGCGCTGACAGCGCCGATGATCATAAAGATCAAAGCCCAGCGGTTCGTAAAGCAGCCAAAGAGTCTGGTGTAG
ATCCTAAAAATGTAGAAGGCACAGGCCGTGGCGGTCGTGTGACTAAGCAAGATATGGCCAGCCCAACACTGAAAGCGGAT
AGCTCTATCAAGTCTGACAGTGGCCGTCCTGTCGCAGAATCAGTAGGTGAGCGTACTGAAAAACGCGTGCCTATGACTCG
TCTACGTAAGACTGTGGCCAACCGTCTGTTAGCCGCTTCTCAAGAAACTGCGATGTTGACCACGTTCAACGAAGTGAATA
TGAAGCCTCTAATGGATCTACGTGCTAAGTACAAAGATCAGTTTGAAAAACGTCACGGCACTCGTCTAGGCTTTATGTCT
ATGTTCGTTAAAGCCGCTACCGAAGCACTAAAACGCTTCCCAGCTGTTAACGCGTCAATCGACGGTGATGACATCGTTTA
TCACGGTTACTATGACGTTGGTGTTGCGGTATCTTCAGACCGTGGTCTAGTGGTGCCTGTACTACGTGATACAGATCGTA
TGAGCATGGCAGACATCGAAGGCGGCATCCGCGACTTCGCTGGTAAAGCCCGTGATGGTAAATTATCACTACAAGAGATG
ACAGGTGGTACCTTCACGATCACTAACGGTGGTGTATTTGGTTCATTAATGTCAACGCCTATCTTGAACCCACCCCAAAC
TGCCATCTTAGGTATGCACGCGATCAATGAGCGCCCAATGGCGGTTAATGGTCAAGTTGAGATCCTACCTATGATGTATC
TAGCGTTATCATACGATCACCGTATGATTGATGGTAAAGATGCGGTACAGTTCTTAGTCACCATCAAGCAGTTGGTTGAA
GACCCAGCAATGCTACTACTTGACCTATAA

Upstream 100 bases:

>100_bases
GCATAAGTTGTTTATTTGCCAATCATAAAAATAATGTGCTTATTTAACCCTTATCAATAAGTCATTAACACTTTATATTA
ATATAACAGGAGCAATTCCC

Downstream 100 bases:

>100_bases
GGTCAACGGGCTTGTATCGGAGAGATTCTGATACAAGCTTTTTCGTTATGGATTGATTGATTATTAAGTTATTTAGCACA
CAGTTTTTTGAGTGATGATT

Product: 2-oxoglutarate dehydrogenase, E2 subunit, dihydrolipoamide succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 409; Mature: 408

Protein sequence:

>409_residues
MAEIKAPVFPESVADGTIVEWHVSEGEQVNRDDILAEIETDKVVLEVVAPDDGVVTKIVKQVDDTVLSDELIAEFEAGAT
GSAAGASEDKAEESSDTATEEKPAAKPAASADSADDHKDQSPAVRKAAKESGVDPKNVEGTGRGGRVTKQDMASPTLKAD
SSIKSDSGRPVAESVGERTEKRVPMTRLRKTVANRLLAASQETAMLTTFNEVNMKPLMDLRAKYKDQFEKRHGTRLGFMS
MFVKAATEALKRFPAVNASIDGDDIVYHGYYDVGVAVSSDRGLVVPVLRDTDRMSMADIEGGIRDFAGKARDGKLSLQEM
TGGTFTITNGGVFGSLMSTPILNPPQTAILGMHAINERPMAVNGQVEILPMMYLALSYDHRMIDGKDAVQFLVTIKQLVE
DPAMLLLDL

Sequences:

>Translated_409_residues
MAEIKAPVFPESVADGTIVEWHVSEGEQVNRDDILAEIETDKVVLEVVAPDDGVVTKIVKQVDDTVLSDELIAEFEAGAT
GSAAGASEDKAEESSDTATEEKPAAKPAASADSADDHKDQSPAVRKAAKESGVDPKNVEGTGRGGRVTKQDMASPTLKAD
SSIKSDSGRPVAESVGERTEKRVPMTRLRKTVANRLLAASQETAMLTTFNEVNMKPLMDLRAKYKDQFEKRHGTRLGFMS
MFVKAATEALKRFPAVNASIDGDDIVYHGYYDVGVAVSSDRGLVVPVLRDTDRMSMADIEGGIRDFAGKARDGKLSLQEM
TGGTFTITNGGVFGSLMSTPILNPPQTAILGMHAINERPMAVNGQVEILPMMYLALSYDHRMIDGKDAVQFLVTIKQLVE
DPAMLLLDL
>Mature_408_residues
AEIKAPVFPESVADGTIVEWHVSEGEQVNRDDILAEIETDKVVLEVVAPDDGVVTKIVKQVDDTVLSDELIAEFEAGATG
SAAGASEDKAEESSDTATEEKPAAKPAASADSADDHKDQSPAVRKAAKESGVDPKNVEGTGRGGRVTKQDMASPTLKADS
SIKSDSGRPVAESVGERTEKRVPMTRLRKTVANRLLAASQETAMLTTFNEVNMKPLMDLRAKYKDQFEKRHGTRLGFMSM
FVKAATEALKRFPAVNASIDGDDIVYHGYYDVGVAVSSDRGLVVPVLRDTDRMSMADIEGGIRDFAGKARDGKLSLQEMT
GGTFTITNGGVFGSLMSTPILNPPQTAILGMHAINERPMAVNGQVEILPMMYLALSYDHRMIDGKDAVQFLVTIKQLVED
PAMLLLDL

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=429, Percent_Identity=27.972027972028, Blast_Score=169, Evalue=3e-42,
Organism=Homo sapiens, GI31711992, Length=433, Percent_Identity=27.9445727482679, Blast_Score=155, Evalue=8e-38,
Organism=Homo sapiens, GI203098753, Length=464, Percent_Identity=25.6465517241379, Blast_Score=122, Evalue=6e-28,
Organism=Homo sapiens, GI203098816, Length=465, Percent_Identity=26.0215053763441, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI260898739, Length=161, Percent_Identity=32.9192546583851, Blast_Score=95, Evalue=1e-19,
Organism=Escherichia coli, GI1786946, Length=409, Percent_Identity=57.2127139364303, Blast_Score=458, Evalue=1e-130,
Organism=Escherichia coli, GI1786305, Length=417, Percent_Identity=31.4148681055156, Blast_Score=150, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI25146366, Length=409, Percent_Identity=41.320293398533, Blast_Score=310, Evalue=9e-85,
Organism=Caenorhabditis elegans, GI17560088, Length=433, Percent_Identity=29.3302540415704, Blast_Score=164, Evalue=6e-41,
Organism=Caenorhabditis elegans, GI17537937, Length=421, Percent_Identity=25.41567695962, Blast_Score=150, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17538894, Length=314, Percent_Identity=28.6624203821656, Blast_Score=133, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6320352, Length=404, Percent_Identity=44.3069306930693, Blast_Score=336, Evalue=5e-93,
Organism=Saccharomyces cerevisiae, GI6324258, Length=454, Percent_Identity=25.3303964757709, Blast_Score=141, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24645909, Length=237, Percent_Identity=56.1181434599156, Blast_Score=277, Evalue=8e-75,
Organism=Drosophila melanogaster, GI18859875, Length=422, Percent_Identity=28.436018957346, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24582497, Length=229, Percent_Identity=31.4410480349345, Blast_Score=128, Evalue=8e-30,
Organism=Drosophila melanogaster, GI20129315, Length=229, Percent_Identity=31.4410480349345, Blast_Score=127, Evalue=1e-29,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 44044; Mature: 43913

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEIKAPVFPESVADGTIVEWHVSEGEQVNRDDILAEIETDKVVLEVVAPDDGVVTKIVK
CCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHEEECCCEEEEEEECCCCHHHHHHHH
QVDDTVLSDELIAEFEAGATGSAAGASEDKAEESSDTATEEKPAAKPAASADSADDHKDQ
HHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
SPAVRKAAKESGVDPKNVEGTGRGGRVTKQDMASPTLKADSSIKSDSGRPVAESVGERTE
CHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHH
KRVPMTRLRKTVANRLLAASQETAMLTTFNEVNMKPLMDLRAKYKDQFEKRHGTRLGFMS
HCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHH
MFVKAATEALKRFPAVNASIDGDDIVYHGYYDVGVAVSSDRGLVVPVLRDTDRMSMADIE
HHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEEEEECCCCEEEEEECCCCCCHHHHHH
GGIRDFAGKARDGKLSLQEMTGGTFTITNGGVFGSLMSTPILNPPQTAILGMHAINERPM
HHHHHHCCCCCCCCEEHEECCCCEEEEECCCHHHHHHHCCCCCCCHHHHHHHHHCCCCCE
AVNGQVEILPMMYLALSYDHRMIDGKDAVQFLVTIKQLVEDPAMLLLDL
EECCCEEHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECC
>Mature Secondary Structure 
AEIKAPVFPESVADGTIVEWHVSEGEQVNRDDILAEIETDKVVLEVVAPDDGVVTKIVK
CCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHEEECCCEEEEEEECCCCHHHHHHHH
QVDDTVLSDELIAEFEAGATGSAAGASEDKAEESSDTATEEKPAAKPAASADSADDHKDQ
HHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
SPAVRKAAKESGVDPKNVEGTGRGGRVTKQDMASPTLKADSSIKSDSGRPVAESVGERTE
CHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHH
KRVPMTRLRKTVANRLLAASQETAMLTTFNEVNMKPLMDLRAKYKDQFEKRHGTRLGFMS
HCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHH
MFVKAATEALKRFPAVNASIDGDDIVYHGYYDVGVAVSSDRGLVVPVLRDTDRMSMADIE
HHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEEEEECCCCEEEEEECCCCCCHHHHHH
GGIRDFAGKARDGKLSLQEMTGGTFTITNGGVFGSLMSTPILNPPQTAILGMHAINERPM
HHHHHHCCCCCCCCEEHEECCCCEEEEECCCHHHHHHHCCCCCCCHHHHHHHHHCCCCCE
AVNGQVEILPMMYLALSYDHRMIDGKDAVQFLVTIKQLVEDPAMLLLDL
EECCCEEHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]