Click here to switch to the map view.

The map label for this gene is truB [H]

Identifier: 148651975

GI number: 148651975

Start: 189456

End: 190490

Strand: Direct

Name: truB [H]

Synonym: PsycPRwf_0159

Alternate gene names: 148651975

Gene position: 189456-190490 (Clockwise)

Preceding gene: 148651974

Following gene: 148651976

Centisome position: 6.36

GC content: 47.73

Gene sequence:

>1035_bases
ATGATGACCGAAGAGATCATTCAATCAGCGCCTGCTGCTCCGCTCAAAAAAAGCCGAGTTTCAGGGGTTATTTTAATCGA
TAAGCCGCAAGGTCTGACCTCGCAGCAGGTGGTATCTAAGGTTAAGTATCTGCTTAAGTCACCTTTGCACGACAGCAAAA
AAGCCGGTCATACTGGGACTCTAGATCCTATGGCGACCGGCTTATTGCCTGTATGCTTAGGGGAGGCGACTAAGTTTAGT
CACTACCAGCTTGATGCTGATAAGGCGTATCAGGCCACTATTAAGCTTGGCATACAAACCGATACTGGAGATGCAGAAGG
GCAGAGCATTGCTACCGCGGCTGTACCTAAGCTAACTGCGGCTGAGCTAGAGGCTGTGGCGCAGCAGTTTATGGGAGATA
TTAAACAAATACCGCCCATGTATTCTGCGCTAAAAAAAGACGGAAAAAAACTGTATGAGTATGCCCGCGCCGGTATTGAG
ATTGATCGTCCGGCACGGCCTATCACTATTCAAGCAATAAGCTTAACCTTGGGCGCGCAGTCCGATGAGCTGATTTTAAA
TGTCACTTGCTCAAAAGGCACCTATGTTCGAGTGCTGGGAGAAGACATTGCCGCAGCGTTAGGCACGCTTGGGCATTTAA
CTGCTCTGCGCCGTACTCAAGTGGGACATTTTGATATTGCCGAAGCAGTAAGTCTTGAGGCGCTTGAGGATCTATCTCAC
GAGCAGCGCTTGGCTTATTTATTGGGTATTGATGCCTGTATCAATGGCATACCGACCCTTGAGGTGTCTGACAGTCAGTG
TGCTCGCCTGCATATGGGGCAACGTCTTAATATCAAGCCTCAGCTTGATGAGGACATCACTAATTATATACAGGCCATGT
TAAGTCAACGGCACAGTGATGCAGATGATAGTGGTAAGGACGAGGCATTAGATTCAGTAGATATCAAGCTTGTGAACCCT
AAAGGCGAGTTTTTAGGCTTGGGGGCAGTAGAGCTAAATGGCCGCTTACAGCCCAAAAAAATGATTCAACGTTAA

Upstream 100 bases:

>100_bases
AATAAGGCAGTGACTAAGACGGAGCAGACATCAGCAGACGATGATGCAGACCGCTTAGACAGTGAAGACCGTTCTTAATC
AAAATCAACTTAGAGTAGAT

Downstream 100 bases:

>100_bases
TGATTGGTTAATGGTAGTGAATACTAGGTAAACTATTAATTACTTTAAAGCTATCAGTAAGTTTTGCAAAATACGGCTCA
TACCCACTGGTATGGGCTTT

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MMTEEIIQSAPAAPLKKSRVSGVILIDKPQGLTSQQVVSKVKYLLKSPLHDSKKAGHTGTLDPMATGLLPVCLGEATKFS
HYQLDADKAYQATIKLGIQTDTGDAEGQSIATAAVPKLTAAELEAVAQQFMGDIKQIPPMYSALKKDGKKLYEYARAGIE
IDRPARPITIQAISLTLGAQSDELILNVTCSKGTYVRVLGEDIAAALGTLGHLTALRRTQVGHFDIAEAVSLEALEDLSH
EQRLAYLLGIDACINGIPTLEVSDSQCARLHMGQRLNIKPQLDEDITNYIQAMLSQRHSDADDSGKDEALDSVDIKLVNP
KGEFLGLGAVELNGRLQPKKMIQR

Sequences:

>Translated_344_residues
MMTEEIIQSAPAAPLKKSRVSGVILIDKPQGLTSQQVVSKVKYLLKSPLHDSKKAGHTGTLDPMATGLLPVCLGEATKFS
HYQLDADKAYQATIKLGIQTDTGDAEGQSIATAAVPKLTAAELEAVAQQFMGDIKQIPPMYSALKKDGKKLYEYARAGIE
IDRPARPITIQAISLTLGAQSDELILNVTCSKGTYVRVLGEDIAAALGTLGHLTALRRTQVGHFDIAEAVSLEALEDLSH
EQRLAYLLGIDACINGIPTLEVSDSQCARLHMGQRLNIKPQLDEDITNYIQAMLSQRHSDADDSGKDEALDSVDIKLVNP
KGEFLGLGAVELNGRLQPKKMIQR
>Mature_344_residues
MMTEEIIQSAPAAPLKKSRVSGVILIDKPQGLTSQQVVSKVKYLLKSPLHDSKKAGHTGTLDPMATGLLPVCLGEATKFS
HYQLDADKAYQATIKLGIQTDTGDAEGQSIATAAVPKLTAAELEAVAQQFMGDIKQIPPMYSALKKDGKKLYEYARAGIE
IDRPARPITIQAISLTLGAQSDELILNVTCSKGTYVRVLGEDIAAALGTLGHLTALRRTQVGHFDIAEAVSLEALEDLSH
EQRLAYLLGIDACINGIPTLEVSDSQCARLHMGQRLNIKPQLDEDITNYIQAMLSQRHSDADDSGKDEALDSVDIKLVNP
KGEFLGLGAVELNGRLQPKKMIQR

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI21040257, Length=221, Percent_Identity=37.10407239819, Blast_Score=132, Evalue=6e-31,
Organism=Homo sapiens, GI215599015, Length=202, Percent_Identity=29.7029702970297, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI4503337, Length=202, Percent_Identity=29.7029702970297, Blast_Score=73, Evalue=3e-13,
Organism=Escherichia coli, GI2367200, Length=332, Percent_Identity=39.1566265060241, Blast_Score=220, Evalue=1e-58,
Organism=Caenorhabditis elegans, GI17553978, Length=208, Percent_Identity=31.25, Blast_Score=84, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6324037, Length=195, Percent_Identity=31.7948717948718, Blast_Score=103, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6323204, Length=284, Percent_Identity=28.5211267605634, Blast_Score=85, Evalue=2e-17,
Organism=Drosophila melanogaster, GI281364189, Length=208, Percent_Identity=28.3653846153846, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI281364187, Length=208, Percent_Identity=28.3653846153846, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI281364185, Length=208, Percent_Identity=28.3653846153846, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI281364183, Length=208, Percent_Identity=28.3653846153846, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI62471759, Length=208, Percent_Identity=28.3653846153846, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI17975520, Length=208, Percent_Identity=28.3653846153846, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR015947
- InterPro:   IPR014780 [H]

Pfam domain/function: PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 37148; Mature: 37148

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTEEIIQSAPAAPLKKSRVSGVILIDKPQGLTSQQVVSKVKYLLKSPLHDSKKAGHTGT
CCCHHHHHCCCCCCCHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCC
LDPMATGLLPVCLGEATKFSHYQLDADKAYQATIKLGIQTDTGDAEGQSIATAAVPKLTA
CCHHHHHHHHHHHCCCCCCCEEEECCCHHEEEEEEEEEEECCCCCCCCEEHHHHCCCHHH
AELEAVAQQFMGDIKQIPPMYSALKKDGKKLYEYARAGIEIDRPARPITIQAISLTLGAQ
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCEECCCCCCEEEEEEEEEECCC
SDELILNVTCSKGTYVRVLGEDIAAALGTLGHLTALRRTQVGHFDIAEAVSLEALEDLSH
CCCEEEEEEECCCCEEEEECHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCH
EQRLAYLLGIDACINGIPTLEVSDSQCARLHMGQRLNIKPQLDEDITNYIQAMLSQRHSD
HHHHHHHHHHHHHHCCCCCEEECCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
ADDSGKDEALDSVDIKLVNPKGEFLGLGAVELNGRLQPKKMIQR
CCCCCCHHHHCCCEEEEECCCCCEEEEEEEEECCCCCHHHHHCC
>Mature Secondary Structure
MMTEEIIQSAPAAPLKKSRVSGVILIDKPQGLTSQQVVSKVKYLLKSPLHDSKKAGHTGT
CCCHHHHHCCCCCCCHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCC
LDPMATGLLPVCLGEATKFSHYQLDADKAYQATIKLGIQTDTGDAEGQSIATAAVPKLTA
CCHHHHHHHHHHHCCCCCCCEEEECCCHHEEEEEEEEEEECCCCCCCCEEHHHHCCCHHH
AELEAVAQQFMGDIKQIPPMYSALKKDGKKLYEYARAGIEIDRPARPITIQAISLTLGAQ
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCEECCCCCCEEEEEEEEEECCC
SDELILNVTCSKGTYVRVLGEDIAAALGTLGHLTALRRTQVGHFDIAEAVSLEALEDLSH
CCCEEEEEEECCCCEEEEECHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCH
EQRLAYLLGIDACINGIPTLEVSDSQCARLHMGQRLNIKPQLDEDITNYIQAMLSQRHSD
HHHHHHHHHHHHHHCCCCCEEECCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
ADDSGKDEALDSVDIKLVNPKGEFLGLGAVELNGRLQPKKMIQR
CCCCCCHHHHCCCEEEEECCCCCEEEEEEEEECCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA