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The map label for this gene is rbfA

Identifier: 148651974

GI number: 148651974

Start: 189011

End: 189433

Strand: Direct

Name: rbfA

Synonym: PsycPRwf_0158

Alternate gene names: 148651974

Gene position: 189011-189433 (Clockwise)

Preceding gene: 148651973

Following gene: 148651975

Centisome position: 6.34

GC content: 45.86

Gene sequence:

>423_bases
ATGAACCAACGTTTACAGCGCTTGGGCGATCAAATTCAGCGTGAACTTGCTGTACTTATCCGTGATGATGTTAATGATCC
TCGTTTGACAGGGTTTGTGACCATCTCGAGTGTCAAAGTCAGCTCTGACTTGGGCTATGCAGACGTGTATGTCACCATCA
TGGAGCCTGAGCTTAATGATGCGATGAGCAAGCACAGTCATGAAGAGAGCCTAAAAGTGCTTAATAAAGCGGCAGGATTT
TTGCGTACTGAGTTAAGTCACAGCTTAAAGACACGTACCACACCTCGCTTAAGATTCCATTATGACGAAGTAACAGCGCG
CGGTAACTACATGATGGACCTGATTAATAAGGCAGTGACTAAGACGGAGCAGACATCAGCAGACGATGATGCAGACCGCT
TAGACAGTGAAGACCGTTCTTAA

Upstream 100 bases:

>100_bases
AGTTGTTCGATAACTCTTTTAGCTATTTTATTATCCGTTATCACAATTTTGGCCAAATAAAGGCCATCTAAGCCTGATAA
CGACATTGAAGGATATAACC

Downstream 100 bases:

>100_bases
TCAAAATCAACTTAGAGTAGATATGATGACCGAAGAGATCATTCAATCAGCGCCTGCTGCTCCGCTCAAAAAAAGCCGAG
TTTCAGGGGTTATTTTAATC

Product: ribosome-binding factor A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 140; Mature: 140

Protein sequence:

>140_residues
MNQRLQRLGDQIQRELAVLIRDDVNDPRLTGFVTISSVKVSSDLGYADVYVTIMEPELNDAMSKHSHEESLKVLNKAAGF
LRTELSHSLKTRTTPRLRFHYDEVTARGNYMMDLINKAVTKTEQTSADDDADRLDSEDRS

Sequences:

>Translated_140_residues
MNQRLQRLGDQIQRELAVLIRDDVNDPRLTGFVTISSVKVSSDLGYADVYVTIMEPELNDAMSKHSHEESLKVLNKAAGF
LRTELSHSLKTRTTPRLRFHYDEVTARGNYMMDLINKAVTKTEQTSADDDADRLDSEDRS
>Mature_140_residues
MNQRLQRLGDQIQRELAVLIRDDVNDPRLTGFVTISSVKVSSDLGYADVYVTIMEPELNDAMSKHSHEESLKVLNKAAGF
LRTELSHSLKTRTTPRLRFHYDEVTARGNYMMDLINKAVTKTEQTSADDDADRLDSEDRS

Specific function: Associates with free 30S ribosomal subunits (but not with 30S subunits that are part of 70S ribosomes or polysomes). Essential for efficient processing of 16S rRNA. May interact with the 5'-terminal helix region of 16S rRNA

COG id: COG0858

COG function: function code J; Ribosome-binding factor A

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rbfA family

Homologues:

Organism=Escherichia coli, GI1789558, Length=127, Percent_Identity=33.8582677165354, Blast_Score=86, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RBFA_PSYWF (A5WBS6)

Other databases:

- EMBL:   CP000713
- RefSeq:   YP_001279067.1
- ProteinModelPortal:   A5WBS6
- SMR:   A5WBS6
- STRING:   A5WBS6
- GeneID:   5206811
- GenomeReviews:   CP000713_GR
- KEGG:   prw:PsycPRwf_0158
- eggNOG:   COG0858
- HOGENOM:   HBG751055
- OMA:   FLRNGLF
- ProtClustDB:   PRK13816
- BioCyc:   PSP56811:PSYCPRWF_0158-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00003
- InterPro:   IPR015946
- InterPro:   IPR000238
- Gene3D:   G3DSA:3.30.300.20
- ProDom:   PD007327
- TIGRFAMs:   TIGR00082

Pfam domain/function: PF02033 RBFA; SSF89919 Rib_bd_factA

EC number: NA

Molecular weight: Translated: 15930; Mature: 15930

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS01319 RBFA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQRLQRLGDQIQRELAVLIRDDVNDPRLTGFVTISSVKVSSDLGYADVYVTIMEPELND
CCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEEECCCCCEEEEEEEEECCCHHH
AMSKHSHEESLKVLNKAAGFLRTELSHSLKTRTTPRLRFHYDEVTARGNYMMDLINKAVT
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHCCCCHHHHHHHHHHH
KTEQTSADDDADRLDSEDRS
HCCCCCCCCCHHHCCCCCCC
>Mature Secondary Structure
MNQRLQRLGDQIQRELAVLIRDDVNDPRLTGFVTISSVKVSSDLGYADVYVTIMEPELND
CCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEEECCCCCEEEEEEEEECCCHHH
AMSKHSHEESLKVLNKAAGFLRTELSHSLKTRTTPRLRFHYDEVTARGNYMMDLINKAVT
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHCCCCHHHHHHHHHHH
KTEQTSADDDADRLDSEDRS
HCCCCCCCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA