Click here to switch to the map view.

The map label for this gene is murF [H]

Identifier: 148651953

GI number: 148651953

Start: 163891

End: 165408

Strand: Direct

Name: murF [H]

Synonym: PsycPRwf_0136

Alternate gene names: 148651953

Gene position: 163891-165408 (Clockwise)

Preceding gene: 148651952

Following gene: 148651954

Centisome position: 5.5

GC content: 50.33

Gene sequence:

>1518_bases
ATGAGCCAACGACACCCCGAGAATGGACTATTTATTTGGACACCTGAGCAGCTACTTGCCGCAACGGCTCAACTAAACGG
GGAGTGGATAGGGCTTGAAGCGGCAGATTCATCGAGCTTACGTGCCACTCGTATCATCACCGATACCCGAAAAATCGAAG
CAGGTGATATCTTCCTTGCCATTGTCGGTGAGAACTTTGATGGACACAGCTTTGTGGCGAGTGCCGTGGAGAAGGGCGCT
ATTGCCACTATTGTCAGTCAGCCGCTATCAGAGGTTAATGTGCCGCAGCTTAAGGTGGATGATACTCGATTGGCTTTTGG
TCAAATTGCCGCTTATCGCCGTCAGCAGCATCCTAATTTAAAGGTGATCGCGTTAACCGGCTCTAGTGGCAAGACCACTG
TCAAAGAAATGCTGCGTCATATCTTTGCTCAAGTTGAAGCCGCTTCTGCCGATGTGCAAAGCACAACTTTGGTGACCCGA
GGTAATCTCAATAACGACTTTGGGGTGCCTATGATGCTGCTAGAATTAGAGCAGCAGCACCGTTATGCAGTGCTTGAGCT
GGGCGCAAACCATGTCGGTGAGATTGCTTATACTGCTGCCATGGTCAAGCCAGATGTGGCCTGCATCCTAAATATTGGGA
CAGCGCATTTGGGAGAGTTTGGTGGCCGTGACAATATCGCCAAGACCAAAGCGGAGATTTATCAGCCGCTGACGCAAGCA
AGTACGGCAGTTGTGCCTTGTGATGATGATTATAGCGATCAATTAATGAGCACCGCCAAGCAGCATACCGATACCATTAT
TGGCTTTGGTTGGCATTCGCTTGAGCAGCGGACGGCAAAGCTGCCTGCAGTCTATGCTACTGAGGTTAAAACCACTGCGC
TACAAAGCCGCTTCCAATTACACTTTGCTATTGAGGGCAAAGCACCTGCCATGGTATCCGTCCTGCTGCCTATGGCCGGC
GAGCACAATGTCAGTAATGCCTTAGCGGCAGCTGCCTGTGCTTACGCCGCTGGCGTGTCGGCTGAATTAATTGCGCAAGG
TCTAAACCAAGCCAAATCCAGCAAAGGCCGACTGGGTAGACAGCGCTTGGGTCAACATCACATCATTGATGATACCTACA
ATGCCAACCCCAATTCTATGCGCGCCGCTGCTGATGTACTGGCGGCTCAAGAAGGCACCAGAATTTTGGTACTGGGTGAC
ATCGGTGAGCTGGGTGATGACGCGGTTAGCGAGCATTATAAATTGGGTGAAGCGATTGCTAACAAAGCGGTGGACTATAT
GTTGTGTGCCGGTGAGTTTGCCAAAGACACGGTAGCTGGAGCCAGCAGTCAAGGCATGACACAGGCATACTCCTTTGATA
CCAAAGCTGACTTATTGCCCAAGCTTATTGAGCTGGTTCAGACGCAACAGCAGCAGGGCCAATTGTGTACCTTATTATTT
AAAGGCTCGCGCACCACCACGATGGAAACCTTGATTGACGATTTAACACAAATTAGCACAAGCCCCTCATCTCTATAG

Upstream 100 bases:

>100_bases
ATTGCGGCGCACAGTTCAGAGGCCTCAAGCCGCTAACCTTATCACCTTCATATTTTATATTTCATATCCTGACCCATTAA
ACCAACGAGACGCAATCATC

Downstream 100 bases:

>100_bases
GTAGCGGGTAGCTTATAACCAAAAATAGCTTATTTAATACGTCGATTGATTCTAGGCTATGTGCATTTTTGGTGAAAAGT
TGGATTAAACCTGTTTTTGT

Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate--D-alanyl-D-alanyl ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 505; Mature: 504

Protein sequence:

>505_residues
MSQRHPENGLFIWTPEQLLAATAQLNGEWIGLEAADSSSLRATRIITDTRKIEAGDIFLAIVGENFDGHSFVASAVEKGA
IATIVSQPLSEVNVPQLKVDDTRLAFGQIAAYRRQQHPNLKVIALTGSSGKTTVKEMLRHIFAQVEAASADVQSTTLVTR
GNLNNDFGVPMMLLELEQQHRYAVLELGANHVGEIAYTAAMVKPDVACILNIGTAHLGEFGGRDNIAKTKAEIYQPLTQA
STAVVPCDDDYSDQLMSTAKQHTDTIIGFGWHSLEQRTAKLPAVYATEVKTTALQSRFQLHFAIEGKAPAMVSVLLPMAG
EHNVSNALAAAACAYAAGVSAELIAQGLNQAKSSKGRLGRQRLGQHHIIDDTYNANPNSMRAAADVLAAQEGTRILVLGD
IGELGDDAVSEHYKLGEAIANKAVDYMLCAGEFAKDTVAGASSQGMTQAYSFDTKADLLPKLIELVQTQQQQGQLCTLLF
KGSRTTTMETLIDDLTQISTSPSSL

Sequences:

>Translated_505_residues
MSQRHPENGLFIWTPEQLLAATAQLNGEWIGLEAADSSSLRATRIITDTRKIEAGDIFLAIVGENFDGHSFVASAVEKGA
IATIVSQPLSEVNVPQLKVDDTRLAFGQIAAYRRQQHPNLKVIALTGSSGKTTVKEMLRHIFAQVEAASADVQSTTLVTR
GNLNNDFGVPMMLLELEQQHRYAVLELGANHVGEIAYTAAMVKPDVACILNIGTAHLGEFGGRDNIAKTKAEIYQPLTQA
STAVVPCDDDYSDQLMSTAKQHTDTIIGFGWHSLEQRTAKLPAVYATEVKTTALQSRFQLHFAIEGKAPAMVSVLLPMAG
EHNVSNALAAAACAYAAGVSAELIAQGLNQAKSSKGRLGRQRLGQHHIIDDTYNANPNSMRAAADVLAAQEGTRILVLGD
IGELGDDAVSEHYKLGEAIANKAVDYMLCAGEFAKDTVAGASSQGMTQAYSFDTKADLLPKLIELVQTQQQQGQLCTLLF
KGSRTTTMETLIDDLTQISTSPSSL
>Mature_504_residues
SQRHPENGLFIWTPEQLLAATAQLNGEWIGLEAADSSSLRATRIITDTRKIEAGDIFLAIVGENFDGHSFVASAVEKGAI
ATIVSQPLSEVNVPQLKVDDTRLAFGQIAAYRRQQHPNLKVIALTGSSGKTTVKEMLRHIFAQVEAASADVQSTTLVTRG
NLNNDFGVPMMLLELEQQHRYAVLELGANHVGEIAYTAAMVKPDVACILNIGTAHLGEFGGRDNIAKTKAEIYQPLTQAS
TAVVPCDDDYSDQLMSTAKQHTDTIIGFGWHSLEQRTAKLPAVYATEVKTTALQSRFQLHFAIEGKAPAMVSVLLPMAGE
HNVSNALAAAACAYAAGVSAELIAQGLNQAKSSKGRLGRQRLGQHHIIDDTYNANPNSMRAAADVLAAQEGTRILVLGDI
GELGDDAVSEHYKLGEAIANKAVDYMLCAGEFAKDTVAGASSQGMTQAYSFDTKADLLPKLIELVQTQQQQGQLCTLLFK
GSRTTTMETLIDDLTQISTSPSSL

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=477, Percent_Identity=40.041928721174, Blast_Score=268, Evalue=5e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 54191; Mature: 54060

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQRHPENGLFIWTPEQLLAATAQLNGEWIGLEAADSSSLRATRIITDTRKIEAGDIFLA
CCCCCCCCCEEEECHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEECCCEEECCCEEEE
IVGENFDGHSFVASAVEKGAIATIVSQPLSEVNVPQLKVDDTRLAFGQIAAYRRQQHPNL
EECCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHCCCCE
KVIALTGSSGKTTVKEMLRHIFAQVEAASADVQSTTLVTRGNLNNDFGVPMMLLELEQQH
EEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHC
RYAVLELGANHVGEIAYTAAMVKPDVACILNIGTAHLGEFGGRDNIAKTKAEIYQPLTQA
CEEEEEECCCHHHHHHHHHHHHCCCEEEEEECCHHHHHCCCCCCCHHHHHHHHHHHHHHC
STAVVPCDDDYSDQLMSTAKQHTDTIIGFGWHSLEQRTAKLPAVYATEVKTTALQSRFQL
CCEEEECCCCHHHHHHHHHHHHCCCEEECCHHHHHHHHHHCCEEEEHHHHHHHHHCCEEE
HFAIEGKAPAMVSVLLPMAGEHNVSNALAAAACAYAAGVSAELIAQGLNQAKSSKGRLGR
EEEECCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
QRLGQHHIIDDTYNANPNSMRAAADVLAAQEGTRILVLGDIGELGDDAVSEHYKLGEAIA
HHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHH
NKAVDYMLCAGEFAKDTVAGASSQGMTQAYSFDTKADLLPKLIELVQTQQQQGQLCTLLF
HHHHHHHEECCHHHHHHHCCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEE
KGSRTTTMETLIDDLTQISTSPSSL
CCCCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SQRHPENGLFIWTPEQLLAATAQLNGEWIGLEAADSSSLRATRIITDTRKIEAGDIFLA
CCCCCCCCEEEECHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEECCCEEECCCEEEE
IVGENFDGHSFVASAVEKGAIATIVSQPLSEVNVPQLKVDDTRLAFGQIAAYRRQQHPNL
EECCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHCCCCE
KVIALTGSSGKTTVKEMLRHIFAQVEAASADVQSTTLVTRGNLNNDFGVPMMLLELEQQH
EEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHC
RYAVLELGANHVGEIAYTAAMVKPDVACILNIGTAHLGEFGGRDNIAKTKAEIYQPLTQA
CEEEEEECCCHHHHHHHHHHHHCCCEEEEEECCHHHHHCCCCCCCHHHHHHHHHHHHHHC
STAVVPCDDDYSDQLMSTAKQHTDTIIGFGWHSLEQRTAKLPAVYATEVKTTALQSRFQL
CCEEEECCCCHHHHHHHHHHHHCCCEEECCHHHHHHHHHHCCEEEEHHHHHHHHHCCEEE
HFAIEGKAPAMVSVLLPMAGEHNVSNALAAAACAYAAGVSAELIAQGLNQAKSSKGRLGR
EEEECCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
QRLGQHHIIDDTYNANPNSMRAAADVLAAQEGTRILVLGDIGELGDDAVSEHYKLGEAIA
HHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHH
NKAVDYMLCAGEFAKDTVAGASSQGMTQAYSFDTKADLLPKLIELVQTQQQQGQLCTLLF
HHHHHHHEECCHHHHHHHCCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEE
KGSRTTTMETLIDDLTQISTSPSSL
CCCCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]