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The map label for this gene is rpfG [H]

Identifier: 148550824

GI number: 148550824

Start: 294496

End: 295836

Strand: Reverse

Name: rpfG [H]

Synonym: Swit_5388

Alternate gene names: 148550824

Gene position: 295836-294496 (Counterclockwise)

Preceding gene: 148550825

Following gene: 148550823

Centisome position: 95.36

GC content: 62.34

Gene sequence:

>1341_bases
ATGAAGCGCCGGATTGCCATCGATCAGATTGAAGCTGGAATGTATCTGGTCGGATTCGAAGGCTTTTGGCTCCTCCACCC
CTTCTGGCGCACAAAATTTGTGCTGACCCAGGCCGATATCAGCGAAATCCGGGCGTCGGGCGTGAAGGCCGTCTTCATCG
ACGACGAGAAAGGCGCCGCTCCGATCTGCGTCACCGCGCCAACGCCCTCCGCCCCTCTCACGCCGCCGCCCGCCCCCGCG
AAACGGATGGAAGCCGCGCGTCACTGGCGCTCCTTCCCCGCGCCTTCGCCCTCGCCCCGACGGCTAATTGTCGATGTCGA
CGAGGTACAGCGCGCCACCGAAGCGATCCAACGGTCGAAGCGGGTGATCATGCGGATGTTCGGCGAGGCGCGCATGGGCA
AGGCGGTGGCGCTACGCGACGTCGAACCCATCGTCGATGAGATCGCGGAATCGGTGATCCGGGATGCTGCAGCCATGATC
AAGGTGACCCGCCTTAAGCGCAAGAACGAGTATACCTATCTCCATTCGGTCGCGGTCTGCGCGCTGATGATCAATCTTGG
CCGTCAACTTGGACTGGCGGAAAGCCTGACGCGCGACCTGGGCATAGCCGGCCTGATGCATGACATCGGCAAGATGTCCG
TTCCGGGCACTGTCCTCGACAAGCCGGGCTCGCTGAACAGTGACGAATTCGAGATCATCCGCGCGCATCCGCAAAAAGGG
CACGAGCTTCTGCTGATGACCGAGGGCATCTCACCCATCGCCCTCGATGTCTGTCTGCACCATCACGAACGCGTCGACGG
CACCGGCTACCCGTTCGGCCTGACCGCGGAGCAGCTCTCACTTCATGCACGCATGGGCGCGGTGTGCGACGTCTATGATG
CGGTAACCTCCCGGCGCCCCTACAAGGACCCTTGGACGCCGTCCGATGCCTTGGCGAAAATGCTCGAATGGGAGGGCCAT
TTCGATCCCGACGTGCTCGATGCCTTCATTTCCAGCATTGGCATCTATCCTGTAGGAACGCTCGTGCGCCTGCGCACCAA
TCGGCTCGGGATCGTCGTCGCCGGTAACGCCCGCGAACCGACCATGCCAGCCGTGCGCGCTTTCTTCTCAACGACGGAGC
GCGAGTTCCTGCCCCCCGAAATCGTGATTTGCTCGGCGACCCTGAAAGGCGATGCCGCGATCGGTATCGAAAACGGCCAG
GCCTGGTTTGGTCCGCGATGGCCGGTCATCCAGGCGTTCGTGCTCGATAACCGCATGCCGACGGCTGATCTGAACGGGGC
CGGCCAATCCATCATCGCTTCGCCAGGCATGGACCAACGGCGCTTCGCAGCGAGAAATTGA

Upstream 100 bases:

>100_bases
GAGGCGCCAAGTGGTCCCGCGAAGGCGCCAATCTGAGCCCACCTGCGACAAGTTAACCGGAATTTAGCCGCGCACGGGGC
AAGTCTCGCGGGGAGGTATC

Downstream 100 bases:

>100_bases
TCGTTCTGGGCGACAACCGCCGCCACTGATGCCGACGGCGTCCTCATCGTCAAATCCCGACGCAAATGTCTGAAGCGCAG
GTCTGGGGTCTGGGAGTGCC

Product: metal dependent phosphohydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 446; Mature: 446

Protein sequence:

>446_residues
MKRRIAIDQIEAGMYLVGFEGFWLLHPFWRTKFVLTQADISEIRASGVKAVFIDDEKGAAPICVTAPTPSAPLTPPPAPA
KRMEAARHWRSFPAPSPSPRRLIVDVDEVQRATEAIQRSKRVIMRMFGEARMGKAVALRDVEPIVDEIAESVIRDAAAMI
KVTRLKRKNEYTYLHSVAVCALMINLGRQLGLAESLTRDLGIAGLMHDIGKMSVPGTVLDKPGSLNSDEFEIIRAHPQKG
HELLLMTEGISPIALDVCLHHHERVDGTGYPFGLTAEQLSLHARMGAVCDVYDAVTSRRPYKDPWTPSDALAKMLEWEGH
FDPDVLDAFISSIGIYPVGTLVRLRTNRLGIVVAGNAREPTMPAVRAFFSTTEREFLPPEIVICSATLKGDAAIGIENGQ
AWFGPRWPVIQAFVLDNRMPTADLNGAGQSIIASPGMDQRRFAARN

Sequences:

>Translated_446_residues
MKRRIAIDQIEAGMYLVGFEGFWLLHPFWRTKFVLTQADISEIRASGVKAVFIDDEKGAAPICVTAPTPSAPLTPPPAPA
KRMEAARHWRSFPAPSPSPRRLIVDVDEVQRATEAIQRSKRVIMRMFGEARMGKAVALRDVEPIVDEIAESVIRDAAAMI
KVTRLKRKNEYTYLHSVAVCALMINLGRQLGLAESLTRDLGIAGLMHDIGKMSVPGTVLDKPGSLNSDEFEIIRAHPQKG
HELLLMTEGISPIALDVCLHHHERVDGTGYPFGLTAEQLSLHARMGAVCDVYDAVTSRRPYKDPWTPSDALAKMLEWEGH
FDPDVLDAFISSIGIYPVGTLVRLRTNRLGIVVAGNAREPTMPAVRAFFSTTEREFLPPEIVICSATLKGDAAIGIENGQ
AWFGPRWPVIQAFVLDNRMPTADLNGAGQSIIASPGMDQRRFAARN
>Mature_446_residues
MKRRIAIDQIEAGMYLVGFEGFWLLHPFWRTKFVLTQADISEIRASGVKAVFIDDEKGAAPICVTAPTPSAPLTPPPAPA
KRMEAARHWRSFPAPSPSPRRLIVDVDEVQRATEAIQRSKRVIMRMFGEARMGKAVALRDVEPIVDEIAESVIRDAAAMI
KVTRLKRKNEYTYLHSVAVCALMINLGRQLGLAESLTRDLGIAGLMHDIGKMSVPGTVLDKPGSLNSDEFEIIRAHPQKG
HELLLMTEGISPIALDVCLHHHERVDGTGYPFGLTAEQLSLHARMGAVCDVYDAVTSRRPYKDPWTPSDALAKMLEWEGH
FDPDVLDAFISSIGIYPVGTLVRLRTNRLGIVVAGNAREPTMPAVRAFFSTTEREFLPPEIVICSATLKGDAAIGIENGQ
AWFGPRWPVIQAFVLDNRMPTADLNGAGQSIIASPGMDQRRFAARN

Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H

COG id: COG2206

COG function: function code T; HD-GYP domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR003607
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 49130; Mature: 49130

Theoretical pI: Translated: 7.64; Mature: 7.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRRIAIDQIEAGMYLVGFEGFWLLHPFWRTKFVLTQADISEIRASGVKAVFIDDEKGAA
CCCCCCHHHHCCCEEEEEECCEEEECHHHHCEEEEEHHHHHHHHHCCEEEEEEECCCCCC
PICVTAPTPSAPLTPPPAPAKRMEAARHWRSFPAPSPSPRRLIVDVDEVQRATEAIQRSK
CEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHHHHHHHH
RVIMRMFGEARMGKAVALRDVEPIVDEIAESVIRDAAAMIKVTRLKRKNEYTYLHSVAVC
HHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
ALMINLGRQLGLAESLTRDLGIAGLMHDIGKMSVPGTVLDKPGSLNSDEFEIIRAHPQKG
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCEEEEECCCCCC
HELLLMTEGISPIALDVCLHHHERVDGTGYPFGLTAEQLSLHARMGAVCDVYDAVTSRRP
CEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHCCCC
YKDPWTPSDALAKMLEWEGHFDPDVLDAFISSIGIYPVGTLVRLRTNRLGIVVAGNAREP
CCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCEEEEEECCCCCC
TMPAVRAFFSTTEREFLPPEIVICSATLKGDAAIGIENGQAWFGPRWPVIQAFVLDNRMP
CHHHHHHHHHHHCCCCCCCCEEEEEEEECCCEEEEECCCCCCCCCCCHHHEEEECCCCCC
TADLNGAGQSIIASPGMDQRRFAARN
CCCCCCCCCCEEECCCCCHHHHCCCC
>Mature Secondary Structure
MKRRIAIDQIEAGMYLVGFEGFWLLHPFWRTKFVLTQADISEIRASGVKAVFIDDEKGAA
CCCCCCHHHHCCCEEEEEECCEEEECHHHHCEEEEEHHHHHHHHHCCEEEEEEECCCCCC
PICVTAPTPSAPLTPPPAPAKRMEAARHWRSFPAPSPSPRRLIVDVDEVQRATEAIQRSK
CEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHHHHHHHH
RVIMRMFGEARMGKAVALRDVEPIVDEIAESVIRDAAAMIKVTRLKRKNEYTYLHSVAVC
HHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
ALMINLGRQLGLAESLTRDLGIAGLMHDIGKMSVPGTVLDKPGSLNSDEFEIIRAHPQKG
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCEEEEECCCCCC
HELLLMTEGISPIALDVCLHHHERVDGTGYPFGLTAEQLSLHARMGAVCDVYDAVTSRRP
CEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHCCCC
YKDPWTPSDALAKMLEWEGHFDPDVLDAFISSIGIYPVGTLVRLRTNRLGIVVAGNAREP
CCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCEEEEEECCCCCC
TMPAVRAFFSTTEREFLPPEIVICSATLKGDAAIGIENGQAWFGPRWPVIQAFVLDNRMP
CHHHHHHHHHHHCCCCCCCCEEEEEEEECCCEEEEECCCCCCCCCCCHHHEEEECCCCCC
TADLNGAGQSIIASPGMDQRRFAARN
CCCCCCCCCCEEECCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11123673 [H]