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The map label for this gene is gdh [H]

Identifier: 148550757

GI number: 148550757

Start: 218227

End: 219576

Strand: Reverse

Name: gdh [H]

Synonym: Swit_5321

Alternate gene names: 148550757

Gene position: 219576-218227 (Counterclockwise)

Preceding gene: 148550758

Following gene: 148550756

Centisome position: 70.78

GC content: 63.33

Gene sequence:

>1350_bases
ATGTCGAAGATCGATGAGAAGCTCGAGCCCGTGCTCGATCAGGTCCTGCGCCGGAATGCAGGAGAACTCGAATTCCACCA
AGCCGTGACCGAGGTCCTCGAAAGCCTGGGCCGCGTCGTCGCGAAATATCCCAGCTATCTCGAGAACGCCCTCATCGAGC
GGATCTGCGAGCCCGAGCGCCAGATCATTTTCCGCGTCCCCTGGGTCGACGACCAGGGCCAGGTGCACATCAACCGCGGC
TTTCGCGTACAGTTCAACTCGGCGCTCGGACCCTATAAGGGCGGCCTTCGCTTTCATCCGTCGGTCAACGTCGGCATCAT
CAAATTCCTCGGCTTCGAGCAGACGTTCAAGAACGCGCTCACCGGCCTTCCAATCGGTGGCGGCAAGGGCGGCTCCGACT
TCGATCCGCGCGGCCGGTCAGACGGCGAAATCATGCGCTTCTGCCAGTCCTACATGACCGAGCTTCATCGTCACCTGGGC
GAATATACCGACGTCCCCGCCGGCGACATCGGCGTGGGCGGACGAGAAATCGGCTATCTCTTCGGCCAGTACAAGCGCCT
GACCAATCGCTACGAAGCCGGCGTCCTTTCTGGCAAGGGCCTGGTCTACGGCGGCTCGCGCGCCCGGACCGAAGCAACCG
GCTACGGCGCGGTCTACTTCGTCGAGCGCATGCTCGCGACCAAGCAACTCACCTTCGATAATCGCCGCGTGGTGGTGTCG
GGCTCGGGCAATGTCGCCATCTACACCGCCGAAAAGATCACCGAATTCGGGGGCAAGGTCGTCGCCTGCTCGGACTCGAG
CGGCTATGTCGTCGATGAGGGCGGCATCGATCTCGCACTCCTCAAGGAGGTCAAGGAAAGCCGCCGGGAACGCATTTCGG
AATATGCCAGGCTCAAGGGCGACGGAGCGCGTTTCTCCGATACCGGCTCCATCTGGGACGTCCCCTGCGAAATCGCGATG
CCATCCGCCACCCAGAACGAGCTCACTGGCCGCGACGCCAAAGCGCTCATCCAGAACGGCGTGATCGCCGTTGGCGAAGG
CGCGAACATGCCCTCGACGCCGGAAGCCGTACGCTTCTTCAGGGAGGCGGGGGTCCTCTTCGCGCCCGGCAAAGCCGCCA
ATGCAGGCGGCGTGGCGACCTCGGCACTGGAAATGCAGCAGAACGCGTCGCGCGACAGCTGGACGTTCGAGCAGACCGAG
CAGCGCCTTGCCGATATCATGCGCCGCATCCATGACACCTGTGCATCGACCGCCGACGAGTTCGGAACGCCTGGCGACTA
TGTCCTTGGCGCAAACATCGCCGGCTTCGTCCGCGTGGCCGAAGCGATGCGGGCGCTTGGCGTCATATAG

Upstream 100 bases:

>100_bases
GCGCGCCTGCCACCCCCACCGCTCTCACCGAAGACACCGCGATGGCCTTTCGTACAGCCTCGCATTAAGCGCTTGAGGGT
GTTCGCCCTTGGGAGAAATC

Downstream 100 bases:

>100_bases
GCCGCCCTGTGCCTACGGCCCGCGGCCCAATGGGCTCTTCGTCGCCGCCCATCAATTGCACAATTCCGCTTCGTGCTCCG
GGCGCGGGGACGTGGTCGTC

Product: glutamate dehydrogenase

Products: NA

Alternate protein names: NADP-GDH [H]

Number of amino acids: Translated: 449; Mature: 448

Protein sequence:

>449_residues
MSKIDEKLEPVLDQVLRRNAGELEFHQAVTEVLESLGRVVAKYPSYLENALIERICEPERQIIFRVPWVDDQGQVHINRG
FRVQFNSALGPYKGGLRFHPSVNVGIIKFLGFEQTFKNALTGLPIGGGKGGSDFDPRGRSDGEIMRFCQSYMTELHRHLG
EYTDVPAGDIGVGGREIGYLFGQYKRLTNRYEAGVLSGKGLVYGGSRARTEATGYGAVYFVERMLATKQLTFDNRRVVVS
GSGNVAIYTAEKITEFGGKVVACSDSSGYVVDEGGIDLALLKEVKESRRERISEYARLKGDGARFSDTGSIWDVPCEIAM
PSATQNELTGRDAKALIQNGVIAVGEGANMPSTPEAVRFFREAGVLFAPGKAANAGGVATSALEMQQNASRDSWTFEQTE
QRLADIMRRIHDTCASTADEFGTPGDYVLGANIAGFVRVAEAMRALGVI

Sequences:

>Translated_449_residues
MSKIDEKLEPVLDQVLRRNAGELEFHQAVTEVLESLGRVVAKYPSYLENALIERICEPERQIIFRVPWVDDQGQVHINRG
FRVQFNSALGPYKGGLRFHPSVNVGIIKFLGFEQTFKNALTGLPIGGGKGGSDFDPRGRSDGEIMRFCQSYMTELHRHLG
EYTDVPAGDIGVGGREIGYLFGQYKRLTNRYEAGVLSGKGLVYGGSRARTEATGYGAVYFVERMLATKQLTFDNRRVVVS
GSGNVAIYTAEKITEFGGKVVACSDSSGYVVDEGGIDLALLKEVKESRRERISEYARLKGDGARFSDTGSIWDVPCEIAM
PSATQNELTGRDAKALIQNGVIAVGEGANMPSTPEAVRFFREAGVLFAPGKAANAGGVATSALEMQQNASRDSWTFEQTE
QRLADIMRRIHDTCASTADEFGTPGDYVLGANIAGFVRVAEAMRALGVI
>Mature_448_residues
SKIDEKLEPVLDQVLRRNAGELEFHQAVTEVLESLGRVVAKYPSYLENALIERICEPERQIIFRVPWVDDQGQVHINRGF
RVQFNSALGPYKGGLRFHPSVNVGIIKFLGFEQTFKNALTGLPIGGGKGGSDFDPRGRSDGEIMRFCQSYMTELHRHLGE
YTDVPAGDIGVGGREIGYLFGQYKRLTNRYEAGVLSGKGLVYGGSRARTEATGYGAVYFVERMLATKQLTFDNRRVVVSG
SGNVAIYTAEKITEFGGKVVACSDSSGYVVDEGGIDLALLKEVKESRRERISEYARLKGDGARFSDTGSIWDVPCEIAMP
SATQNELTGRDAKALIQNGVIAVGEGANMPSTPEAVRFFREAGVLFAPGKAANAGGVATSALEMQQNASRDSWTFEQTEQ
RLADIMRRIHDTCASTADEFGTPGDYVLGANIAGFVRVAEAMRALGVI

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=357, Percent_Identity=29.9719887955182, Blast_Score=159, Evalue=4e-39,
Organism=Homo sapiens, GI4885281, Length=357, Percent_Identity=29.4117647058824, Blast_Score=157, Evalue=2e-38,
Organism=Escherichia coli, GI1788059, Length=445, Percent_Identity=58.876404494382, Blast_Score=508, Evalue=1e-145,
Organism=Caenorhabditis elegans, GI17544676, Length=363, Percent_Identity=28.3746556473829, Blast_Score=124, Evalue=8e-29,
Organism=Saccharomyces cerevisiae, GI6319256, Length=450, Percent_Identity=49.5555555555556, Blast_Score=386, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6324951, Length=448, Percent_Identity=48.2142857142857, Blast_Score=377, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24649283, Length=418, Percent_Identity=27.0334928229665, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI45549226, Length=358, Percent_Identity=27.6536312849162, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI45553475, Length=358, Percent_Identity=27.6536312849162, Blast_Score=132, Evalue=5e-31,
Organism=Drosophila melanogaster, GI24649460, Length=294, Percent_Identity=27.5510204081633, Blast_Score=109, Evalue=5e-24,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.4 [H]

Molecular weight: Translated: 48951; Mature: 48820

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKIDEKLEPVLDQVLRRNAGELEFHQAVTEVLESLGRVVAKYPSYLENALIERICEPER
CCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
QIIFRVPWVDDQGQVHINRGFRVQFNSALGPYKGGLRFHPSVNVGIIKFLGFEQTFKNAL
EEEEECCCCCCCCCEEECCCEEEEECCCCCCCCCCEEECCCCCCHHHHHHCHHHHHHHHH
TGLPIGGGKGGSDFDPRGRSDGEIMRFCQSYMTELHRHLGEYTDVPAGDIGVGGREIGYL
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
FGQYKRLTNRYEAGVLSGKGLVYGGSRARTEATGYGAVYFVERMLATKQLTFDNRRVVVS
HHHHHHHHHHHHCCEECCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHEEECCCEEEEE
GSGNVAIYTAEKITEFGGKVVACSDSSGYVVDEGGIDLALLKEVKESRRERISEYARLKG
CCCCEEEEEHHHHHHHCCEEEEEECCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCC
DGARFSDTGSIWDVPCEIAMPSATQNELTGRDAKALIQNGVIAVGEGANMPSTPEAVRFF
CCCCCCCCCCEEECCCCEECCCCCCHHCCCCHHHHHHHCCCEEEECCCCCCCCHHHHHHH
REAGVLFAPGKAANAGGVATSALEMQQNASRDSWTFEQTEQRLADIMRRIHDTCASTADE
HHCCCEECCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
FGTPGDYVLGANIAGFVRVAEAMRALGVI
CCCCCCEEECCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SKIDEKLEPVLDQVLRRNAGELEFHQAVTEVLESLGRVVAKYPSYLENALIERICEPER
CHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
QIIFRVPWVDDQGQVHINRGFRVQFNSALGPYKGGLRFHPSVNVGIIKFLGFEQTFKNAL
EEEEECCCCCCCCCEEECCCEEEEECCCCCCCCCCEEECCCCCCHHHHHHCHHHHHHHHH
TGLPIGGGKGGSDFDPRGRSDGEIMRFCQSYMTELHRHLGEYTDVPAGDIGVGGREIGYL
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
FGQYKRLTNRYEAGVLSGKGLVYGGSRARTEATGYGAVYFVERMLATKQLTFDNRRVVVS
HHHHHHHHHHHHCCEECCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHEEECCCEEEEE
GSGNVAIYTAEKITEFGGKVVACSDSSGYVVDEGGIDLALLKEVKESRRERISEYARLKG
CCCCEEEEEHHHHHHHCCEEEEEECCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCC
DGARFSDTGSIWDVPCEIAMPSATQNELTGRDAKALIQNGVIAVGEGANMPSTPEAVRFF
CCCCCCCCCCEEECCCCEECCCCCCHHCCCCHHHHHHHCCCEEEECCCCCCCCHHHHHHH
REAGVLFAPGKAANAGGVATSALEMQQNASRDSWTFEQTEQRLADIMRRIHDTCASTADE
HHCCCEECCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
FGTPGDYVLGANIAGFVRVAEAMRALGVI
CCCCCCEEECCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12840036 [H]