Click here to switch to the map view.

The map label for this gene is aes [H]

Identifier: 148550722

GI number: 148550722

Start: 185894

End: 186982

Strand: Reverse

Name: aes [H]

Synonym: Swit_5286

Alternate gene names: 148550722

Gene position: 186982-185894 (Counterclockwise)

Preceding gene: 148550727

Following gene: 148550714

Centisome position: 60.27

GC content: 68.23

Gene sequence:

>1089_bases
ATGCGTAAAGCGCCTTTGCTCGCCGCCGGCACGCTTGTCTCCGCGGCTCTTGCCTTCGCCGCGCTCGGGCCCGTAGCGGC
GCCGGTTTTGGCGGCGGCGGCGCAGGGCCAGCCGGGCATCCCGGCGAGCGGCGCCAAGGTCGACGCCGACAACCAGGCCG
TTCTCGATGCCTTGAAAGGGCTTGGCCTGCGGCCCTACCACACGCTCAGTCCGGCCGAAGCGCGCCTGCAACCGAGCTTC
GCCGACGGGGTAAAGGCCGTGCTCCAGAAGCAGGGTCGCCCGGTTGCGCCTCCACCCGGCGTCGCGACGCAGGATATCCA
GGTAGCCGGCGGGGCGGGTCCGATCCCAGCACGGGTCTATCGACCGGCCGACGCACAAGGTCCCCTTCCCGTCATCGTCT
ACTACCATGGCGGCGGATGGGTGCTCGCGACCATCGACACCTATGATGCCTCGGCCCGTGCCCTCGCCCGAGAAGCGCGC
GCGATCGTGATCTCGGTCGAATATCGCAAGGCGCCCGAGGCGAAATTCCCGGCTCAGCACGACGACGCCCTCGCGGCATA
CCGCTGGGCGCTCGGCCATGCCGGCGAGATCGGTGGTGACCCGCGCAAGATCGCGCTCGCGGGCGAAAGCGCCGGCGGCA
ACCTCGCCCTCGCGACCGCGATCGCCGCGCGCGATGCCAAGTTGCAAAAGCCCGCGCATGTATTGACCATCTATCCGATC
GCCGGTTCCGACCTCAACACCCCCTCCTATCAGGAAAACGCGAATGCCATGCCGCTCAATCGGGCGGCCATGGCCTGGTT
CCTCCATCACACGACGCGCGGCCCGGCTGACGCAACCGATCCGCGCATCAATCTCGTCGCGGCAAACCTGCGCGGTCTCC
CGCCCGTGACCCTCATCCAGGCCCAGATCGATCCGTTGACGTCCGAGGGCCAGACGCTTGCAGGCCGGCTGCGCGCCGCG
GGTGTGCCGGTTGCAACGCAGCTCTATCCTGGCGTTACGCATGAGTTCTTCGGCGCCGATGCGGTGATCCGTAAAGCTGG
AGAAGCCCAGCGCTTCGCCGGCACACGGCTGAAGGCGAGCTTTCGTTGA

Upstream 100 bases:

>100_bases
TCGCACATGATTTATTTCCGCAGCTGGAATGACTTAGGGAACGGGCGTGCGCAGCGCCCGTTCACCTGTCGCAACTTCAT
GCAACAGGGAAACACTTTTC

Downstream 100 bases:

>100_bases
AGGCGGGCCGCCCGCAGCCGGGCGGCCCCACCTGACACCCCCCAGCGCTACGGCGCGCGCCTCGGTCGTCGGCCGCAGCT
AATATTGGCGCGGACAGATC

Product: alpha/beta hydrolase domain-containing protein

Products: alcohol; a carboxylate [C]

Alternate protein names: NA

Number of amino acids: Translated: 362; Mature: 362

Protein sequence:

>362_residues
MRKAPLLAAGTLVSAALAFAALGPVAAPVLAAAAQGQPGIPASGAKVDADNQAVLDALKGLGLRPYHTLSPAEARLQPSF
ADGVKAVLQKQGRPVAPPPGVATQDIQVAGGAGPIPARVYRPADAQGPLPVIVYYHGGGWVLATIDTYDASARALAREAR
AIVISVEYRKAPEAKFPAQHDDALAAYRWALGHAGEIGGDPRKIALAGESAGGNLALATAIAARDAKLQKPAHVLTIYPI
AGSDLNTPSYQENANAMPLNRAAMAWFLHHTTRGPADATDPRINLVAANLRGLPPVTLIQAQIDPLTSEGQTLAGRLRAA
GVPVATQLYPGVTHEFFGADAVIRKAGEAQRFAGTRLKASFR

Sequences:

>Translated_362_residues
MRKAPLLAAGTLVSAALAFAALGPVAAPVLAAAAQGQPGIPASGAKVDADNQAVLDALKGLGLRPYHTLSPAEARLQPSF
ADGVKAVLQKQGRPVAPPPGVATQDIQVAGGAGPIPARVYRPADAQGPLPVIVYYHGGGWVLATIDTYDASARALAREAR
AIVISVEYRKAPEAKFPAQHDDALAAYRWALGHAGEIGGDPRKIALAGESAGGNLALATAIAARDAKLQKPAHVLTIYPI
AGSDLNTPSYQENANAMPLNRAAMAWFLHHTTRGPADATDPRINLVAANLRGLPPVTLIQAQIDPLTSEGQTLAGRLRAA
GVPVATQLYPGVTHEFFGADAVIRKAGEAQRFAGTRLKASFR
>Mature_362_residues
MRKAPLLAAGTLVSAALAFAALGPVAAPVLAAAAQGQPGIPASGAKVDADNQAVLDALKGLGLRPYHTLSPAEARLQPSF
ADGVKAVLQKQGRPVAPPPGVATQDIQVAGGAGPIPARVYRPADAQGPLPVIVYYHGGGWVLATIDTYDASARALAREAR
AIVISVEYRKAPEAKFPAQHDDALAAYRWALGHAGEIGGDPRKIALAGESAGGNLALATAIAARDAKLQKPAHVLTIYPI
AGSDLNTPSYQENANAMPLNRAAMAWFLHHTTRGPADATDPRINLVAANLRGLPPVTLIQAQIDPLTSEGQTLAGRLRAA
GVPVATQLYPGVTHEFFGADAVIRKAGEAQRFAGTRLKASFR

Specific function: Displays esterase activity towards short chain fatty esters (acyl chain length of up to 8 carbons). Able to hydrolyze triacetylglycerol (triacetin) and tributyrylglycerol (tributyrin), but not trioleylglycerol (triolein) or cholesterol oleate. Negatively

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI68051721, Length=178, Percent_Identity=36.5168539325843, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI68299767, Length=204, Percent_Identity=33.8235294117647, Blast_Score=95, Evalue=9e-20,
Organism=Homo sapiens, GI206597554, Length=276, Percent_Identity=27.1739130434783, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI21328446, Length=119, Percent_Identity=36.9747899159664, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI61966717, Length=171, Percent_Identity=32.7485380116959, Blast_Score=86, Evalue=8e-17,
Organism=Homo sapiens, GI157041239, Length=176, Percent_Identity=30.6818181818182, Blast_Score=85, Evalue=9e-17,
Organism=Homo sapiens, GI226423947, Length=186, Percent_Identity=34.9462365591398, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI226423952, Length=134, Percent_Identity=35.0746268656716, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI226423950, Length=134, Percent_Identity=35.0746268656716, Blast_Score=72, Evalue=6e-13,
Organism=Escherichia coli, GI1786682, Length=257, Percent_Identity=30.3501945525292, Blast_Score=107, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI71996133, Length=245, Percent_Identity=31.0204081632653, Blast_Score=88, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17540028, Length=143, Percent_Identity=37.0629370629371, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI72001146, Length=144, Percent_Identity=34.7222222222222, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI115533412, Length=121, Percent_Identity=31.404958677686, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI115533410, Length=121, Percent_Identity=31.404958677686, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.- [C]

Molecular weight: Translated: 37426; Mature: 37426

Theoretical pI: Translated: 10.09; Mature: 10.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKAPLLAAGTLVSAALAFAALGPVAAPVLAAAAQGQPGIPASGAKVDADNQAVLDALKG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCHHHHHHHHC
LGLRPYHTLSPAEARLQPSFADGVKAVLQKQGRPVAPPPGVATQDIQVAGGAGPIPARVY
CCCCCCCCCCCHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCEEE
RPADAQGPLPVIVYYHGGGWVLATIDTYDASARALAREARAIVISVEYRKAPEAKFPAQH
CCCCCCCCCEEEEEEECCCEEEEEEECCCCHHHHHHHCCEEEEEEEEECCCCCCCCCCCC
DDALAAYRWALGHAGEIGGDPRKIALAGESAGGNLALATAIAARDAKLQKPAHVLTIYPI
CCHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCEEEEEHHHHHCCCCCCCCEEEEEEEE
AGSDLNTPSYQENANAMPLNRAAMAWFLHHTTRGPADATDPRINLVAANLRGLPPVTLIQ
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCEEEEE
AQIDPLTSEGQTLAGRLRAAGVPVATQLYPGVTHEFFGADAVIRKAGEAQRFAGTRLKAS
EECCCCCCCCHHHHHHHHHCCCCCHHHHCCCCCHHHHCHHHHHHHCCCHHHHCCCCEEEC
FR
CC
>Mature Secondary Structure
MRKAPLLAAGTLVSAALAFAALGPVAAPVLAAAAQGQPGIPASGAKVDADNQAVLDALKG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCHHHHHHHHC
LGLRPYHTLSPAEARLQPSFADGVKAVLQKQGRPVAPPPGVATQDIQVAGGAGPIPARVY
CCCCCCCCCCCHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCEEE
RPADAQGPLPVIVYYHGGGWVLATIDTYDASARALAREARAIVISVEYRKAPEAKFPAQH
CCCCCCCCCEEEEEEECCCEEEEEEECCCCHHHHHHHCCEEEEEEEEECCCCCCCCCCCC
DDALAAYRWALGHAGEIGGDPRKIALAGESAGGNLALATAIAARDAKLQKPAHVLTIYPI
CCHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCEEEEEHHHHHCCCCCCCCEEEEEEEE
AGSDLNTPSYQENANAMPLNRAAMAWFLHHTTRGPADATDPRINLVAANLRGLPPVTLIQ
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCEEEEE
AQIDPLTSEGQTLAGRLRAAGVPVATQLYPGVTHEFFGADAVIRKAGEAQRFAGTRLKAS
EECCCCCCCCHHHHHHHHHCCCCCHHHHCCCCCHHHHCHHHHHHHCCCHHHHCCCCEEEC
FR
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Carboxylic ester; H2O [C]

Specific reaction: Carboxylic ester + H2O = an alcohol + carboxylate [C]

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA