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The map label for this gene is mprF [H]

Identifier: 148550702

GI number: 148550702

Start: 160490

End: 162709

Strand: Direct

Name: mprF [H]

Synonym: Swit_5266

Alternate gene names: 148550702

Gene position: 160490-162709 (Clockwise)

Preceding gene: 148550700

Following gene: 148550703

Centisome position: 51.73

GC content: 68.47

Gene sequence:

>2220_bases
GTGATCGGAAAAGCGCAAATGGACCGGCTCAAGAGCGGCGTCCCGGTGGCGGTGGTTCTCCTCCTTGCGGCGATGGGCTT
CTTCGCACTGTCGCACCTCCTGCGCGAAGTGCACATGCACGACATCCGGGCAGCTTGGGCGCAAGTGGGCGTCCTTCATC
TGCTCGCGGCGGCGGGACTTACCGCAATCAGCTATCTGGCGTTGACGCTCTACGACGTGCTGGCGCTGCGCGCGATCGAT
CGTCCCCTGCCCTATCGCGTCGCGGCGCTGGCCTCGTTCACAAGCTATACGCTCAGCCATAATCTGGGTCTGGCGCTCTT
CACCGGCGGCTCGGCCCGCTACCGGATCTATAGCGCGCACGGCCTGGGCGCGGGCGATGTGGGCCGCGTGGTCGCGATCG
CCGGGCTGACCTTCTGGGGCGGCGTCTTCACCCTTGCCGGGGCGATGCTGGCGCTCTTTCCCGGCGGCCTTGGCTTTGCC
GGCTTCGCGCCCTCGCCCTGGGTCTGGCGAGCAAGTGGCCTCGCGCTCCTTGCCCTCATCATCGGTGCGCTTGCCTGGTC
AGGACCCTCGGGCCGGACTGTCCGGATCTGGAAATGGGATTTGCGGATCCCGGGCTACCGGACGGCGCTCGCCCAGCTTG
GCGTCGCCACGGTCGATCTCATGGCCGCGAGCGCGGCCCTGCTGGTGCTGGTCCCGGGCGCTAGTCTTGCCGACTGGCCG
GTCTTCTTCATCGGCTACATATTGGCGATCATTGCGGTACTCGTAACCCACGTGCCGGGCGGCCTCGGCGTTTTCGAAGC
GGTCATCGTGGCAGCCCTGCCGGCAACGCCGCGCCCGGAGCTCATCGCCGCGCTGCTCGGCTACCGGCTGGTCTACTATA
TCCTGCCCCTGCTGATCGCAGGGGTCATCATCGCCGTGCAGGAAGGCGCGCGCTGGCGGCGGCCGATGGCTGCGACGTTG
CACGGCGCCCAGACGATCGCCTCGGGCATAGCGCCGACGCTGGTCGCGGCCCTTGTCTTCCTGGGCGGCATGGTCCTGCT
GGTCTCGGGCGCGCTGCCCGCCGAGCATGATCGCATGGCGGCGCTGGCAGCGATCCTGCCCTTGCCGCTGGTGGAAATAT
CGCATCTCGCCGGCAGCGTCGTCGGCGCCGCGCTGCTCCTGGTGTCGGCGGGCCTTTATCGTCGCCTCGATGGGGCGGCC
TGGCTGACAGGCCTGCTGCTGCTCATCGGCGCGCTGGTCTCGCTCGCCAAGGGCCTCGACTATGAGGAGGCGATCCTCAT
GCTGCTGCTCGCGGGCGTCCTGCGCTGGACGCGGGGCGCCTTCTACCGCCGGACGCGGCTGACGCAGGAAGTGCTGACGC
CGAGCTGGCTCGCGAGCGTGGCGCTGATGATCGGCCTGCCGATCTGGGTCGGCTTCTTCGCCTATAAGCGCGTGCCCTAC
AGCACCGATCTCTTCTGGCGCTTTTCGGAGCATGGCGACGCCTCGCGCTTCCTGCGCGCCAGCCTCGGCGTGGGCCTCGC
GCTGGCCGCCCTCGCAGTCTGGCGGCTGCTGCGCCCGGCCCCCGTGGCGGGTCCGGGGCTCGTCAGCCTGGAGCCGCCGC
AGGGCGAAGCGATGGCGCTCACGCACCGCACCGATGCCAATCTCGCCTATACGGGCGACAAGCGGTTCCTGTGCTCGCCT
TCGGGCCGCGCCTTTGTCATGTACCAGGTGCGCGGGGCAAGCTGGATCGTGATGGGCGATCCGGTCGGGGATCCCGCCGA
ATGGAGCGAACTCCTCTGGCAGCTGCGCGAAGGGGCGGACGCGGCGCAGGGCCGCTTGCTACTCTACCAGATCAGCCTGC
AGGCCTTGCCCGTGGCGATCGACCTGGGCTTGCAGATCGTCAAATATGGCGAAGAAGCGCGGGTCGATCTTGCCGCCTTC
ACGCTCGACACGCCGTCGGCCAAGCCGCTGCGCTATGCGGTGCGCCGCGCGGAGAAGGAAGGCGCGCGCTTCGCGATCAT
CGCGCGAGACGAGCTTGCCGCGCATCTGCCGCGGCTGCAGGAGATTTCCGAAAGCTGGCTGCGCGAGAAGGGCCACCGGG
AAAAGGGGTTCAGCGTTGGCCGCTTCGACCCGCCTATCTCGCCCGCTTCGACTGCGCGGTCGTCCTGCGCGAGGAGAAGA
TTGTCGCCTTCGCCAATATCTGGGCGACGCAGGATCGCTCCGAGCTGTCGATCGACCTGA

Upstream 100 bases:

>100_bases
AGCGGGATGCCGGCGGCGGCACCCGTAAACGCCGCTCCAATTTCGCTGTTCGGCGCCGCGCCGTCTCCATAAGGTGGTCC
CGTCTTCGAGAGGGGTGCGG

Downstream 100 bases:

>100_bases
TGCGCCACGATGCCGACATGCCCTATGGCACGATGGACTATCTGTTCGTGCGGCTGATGCAATGGGGCCATGGGCAGGGC
TATCGCTGGTTCACCCTCGG

Product: hypothetical protein

Products: NA

Alternate protein names: Lysylphosphatidylglycerol synthase; LPG synthase; Multiple peptide resistance factor [H]

Number of amino acids: Translated: 739; Mature: 739

Protein sequence:

>739_residues
MIGKAQMDRLKSGVPVAVVLLLAAMGFFALSHLLREVHMHDIRAAWAQVGVLHLLAAAGLTAISYLALTLYDVLALRAID
RPLPYRVAALASFTSYTLSHNLGLALFTGGSARYRIYSAHGLGAGDVGRVVAIAGLTFWGGVFTLAGAMLALFPGGLGFA
GFAPSPWVWRASGLALLALIIGALAWSGPSGRTVRIWKWDLRIPGYRTALAQLGVATVDLMAASAALLVLVPGASLADWP
VFFIGYILAIIAVLVTHVPGGLGVFEAVIVAALPATPRPELIAALLGYRLVYYILPLLIAGVIIAVQEGARWRRPMAATL
HGAQTIASGIAPTLVAALVFLGGMVLLVSGALPAEHDRMAALAAILPLPLVEISHLAGSVVGAALLLVSAGLYRRLDGAA
WLTGLLLLIGALVSLAKGLDYEEAILMLLLAGVLRWTRGAFYRRTRLTQEVLTPSWLASVALMIGLPIWVGFFAYKRVPY
STDLFWRFSEHGDASRFLRASLGVGLALAALAVWRLLRPAPVAGPGLVSLEPPQGEAMALTHRTDANLAYTGDKRFLCSP
SGRAFVMYQVRGASWIVMGDPVGDPAEWSELLWQLREGADAAQGRLLLYQISLQALPVAIDLGLQIVKYGEEARVDLAAF
TLDTPSAKPLRYAVRRAEKEGARFAIIARDELAAHLPRLQEISESWLREKGHREKGFSVGRFDPPISPASTARSSCARRR
LSPSPISGRRRIAPSCRST

Sequences:

>Translated_739_residues
MIGKAQMDRLKSGVPVAVVLLLAAMGFFALSHLLREVHMHDIRAAWAQVGVLHLLAAAGLTAISYLALTLYDVLALRAID
RPLPYRVAALASFTSYTLSHNLGLALFTGGSARYRIYSAHGLGAGDVGRVVAIAGLTFWGGVFTLAGAMLALFPGGLGFA
GFAPSPWVWRASGLALLALIIGALAWSGPSGRTVRIWKWDLRIPGYRTALAQLGVATVDLMAASAALLVLVPGASLADWP
VFFIGYILAIIAVLVTHVPGGLGVFEAVIVAALPATPRPELIAALLGYRLVYYILPLLIAGVIIAVQEGARWRRPMAATL
HGAQTIASGIAPTLVAALVFLGGMVLLVSGALPAEHDRMAALAAILPLPLVEISHLAGSVVGAALLLVSAGLYRRLDGAA
WLTGLLLLIGALVSLAKGLDYEEAILMLLLAGVLRWTRGAFYRRTRLTQEVLTPSWLASVALMIGLPIWVGFFAYKRVPY
STDLFWRFSEHGDASRFLRASLGVGLALAALAVWRLLRPAPVAGPGLVSLEPPQGEAMALTHRTDANLAYTGDKRFLCSP
SGRAFVMYQVRGASWIVMGDPVGDPAEWSELLWQLREGADAAQGRLLLYQISLQALPVAIDLGLQIVKYGEEARVDLAAF
TLDTPSAKPLRYAVRRAEKEGARFAIIARDELAAHLPRLQEISESWLREKGHREKGFSVGRFDPPISPASTARSSCARRR
LSPSPISGRRRIAPSCRST
>Mature_739_residues
MIGKAQMDRLKSGVPVAVVLLLAAMGFFALSHLLREVHMHDIRAAWAQVGVLHLLAAAGLTAISYLALTLYDVLALRAID
RPLPYRVAALASFTSYTLSHNLGLALFTGGSARYRIYSAHGLGAGDVGRVVAIAGLTFWGGVFTLAGAMLALFPGGLGFA
GFAPSPWVWRASGLALLALIIGALAWSGPSGRTVRIWKWDLRIPGYRTALAQLGVATVDLMAASAALLVLVPGASLADWP
VFFIGYILAIIAVLVTHVPGGLGVFEAVIVAALPATPRPELIAALLGYRLVYYILPLLIAGVIIAVQEGARWRRPMAATL
HGAQTIASGIAPTLVAALVFLGGMVLLVSGALPAEHDRMAALAAILPLPLVEISHLAGSVVGAALLLVSAGLYRRLDGAA
WLTGLLLLIGALVSLAKGLDYEEAILMLLLAGVLRWTRGAFYRRTRLTQEVLTPSWLASVALMIGLPIWVGFFAYKRVPY
STDLFWRFSEHGDASRFLRASLGVGLALAALAVWRLLRPAPVAGPGLVSLEPPQGEAMALTHRTDANLAYTGDKRFLCSP
SGRAFVMYQVRGASWIVMGDPVGDPAEWSELLWQLREGADAAQGRLLLYQISLQALPVAIDLGLQIVKYGEEARVDLAAF
TLDTPSAKPLRYAVRRAEKEGARFAIIARDELAAHLPRLQEISESWLREKGHREKGFSVGRFDPPISPASTARSSCARRR
LSPSPISGRRRIAPSCRST

Specific function: Catalyzes the transfer of a lysyl group from L-lysyl- tRNA(Lys) to membrane-bound phosphatidylglycerol (PG), which produces lysylphosphatidylglycerol (LPG), a major component of the bacterial membrane with a positive net charge. LPG synthesis contributes

COG id: COG2898

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LPG synthase family [H]

Homologues:

Organism=Escherichia coli, GI1787006, Length=257, Percent_Identity=34.2412451361868, Blast_Score=100, Evalue=5e-22,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016181
- InterPro:   IPR022791 [H]

Pfam domain/function: PF03706 UPF0104 [H]

EC number: =2.3.2.3 [H]

Molecular weight: Translated: 78984; Mature: 78984

Theoretical pI: Translated: 10.37; Mature: 10.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGKAQMDRLKSGVPVAVVLLLAAMGFFALSHLLREVHMHDIRAAWAQVGVLHLLAAAGL
CCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TAISYLALTLYDVLALRAIDRPLPYRVAALASFTSYTLSHNLGLALFTGGSARYRIYSAH
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEEEC
GLGAGDVGRVVAIAGLTFWGGVFTLAGAMLALFPGGLGFAGFAPSPWVWRASGLALLALI
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEECHHHHHHHHHH
IGALAWSGPSGRTVRIWKWDLRIPGYRTALAQLGVATVDLMAASAALLVLVPGASLADWP
HHHHHCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCHHH
VFFIGYILAIIAVLVTHVPGGLGVFEAVIVAALPATPRPELIAALLGYRLVYYILPLLIA
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
GVIIAVQEGARWRRPMAATLHGAQTIASGIAPTLVAALVFLGGMVLLVSGALPAEHDRMA
HHHHHHHCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
ALAAILPLPLVEISHLAGSVVGAALLLVSAGLYRRLDGAAWLTGLLLLIGALVSLAKGLD
HHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
YEEAILMLLLAGVLRWTRGAFYRRTRLTQEVLTPSWLASVALMIGLPIWVGFFAYKRVPY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
STDLFWRFSEHGDASRFLRASLGVGLALAALAVWRLLRPAPVAGPGLVSLEPPQGEAMAL
CCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCEEEE
THRTDANLAYTGDKRFLCSPSGRAFVMYQVRGASWIVMGDPVGDPAEWSELLWQLREGAD
EECCCCCEEEECCCEEEECCCCCEEEEEEECCCEEEEECCCCCCHHHHHHHHHHHHCCCC
AAQGRLLLYQISLQALPVAIDLGLQIVKYGEEARVDLAAFTLDTPSAKPLRYAVRRAEKE
HHHCCEEEEEEEHHHHCCHHHCCHHHHHCCCHHCEEEEEEEECCCCCCHHHHHHHHHHHC
GARFAIIARDELAAHLPRLQEISESWLREKGHREKGFSVGRFDPPISPASTARSSCARRR
CCEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHC
LSPSPISGRRRIAPSCRST
CCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MIGKAQMDRLKSGVPVAVVLLLAAMGFFALSHLLREVHMHDIRAAWAQVGVLHLLAAAGL
CCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TAISYLALTLYDVLALRAIDRPLPYRVAALASFTSYTLSHNLGLALFTGGSARYRIYSAH
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEEEC
GLGAGDVGRVVAIAGLTFWGGVFTLAGAMLALFPGGLGFAGFAPSPWVWRASGLALLALI
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEECHHHHHHHHHH
IGALAWSGPSGRTVRIWKWDLRIPGYRTALAQLGVATVDLMAASAALLVLVPGASLADWP
HHHHHCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCHHH
VFFIGYILAIIAVLVTHVPGGLGVFEAVIVAALPATPRPELIAALLGYRLVYYILPLLIA
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
GVIIAVQEGARWRRPMAATLHGAQTIASGIAPTLVAALVFLGGMVLLVSGALPAEHDRMA
HHHHHHHCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
ALAAILPLPLVEISHLAGSVVGAALLLVSAGLYRRLDGAAWLTGLLLLIGALVSLAKGLD
HHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
YEEAILMLLLAGVLRWTRGAFYRRTRLTQEVLTPSWLASVALMIGLPIWVGFFAYKRVPY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
STDLFWRFSEHGDASRFLRASLGVGLALAALAVWRLLRPAPVAGPGLVSLEPPQGEAMAL
CCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCEEEE
THRTDANLAYTGDKRFLCSPSGRAFVMYQVRGASWIVMGDPVGDPAEWSELLWQLREGAD
EECCCCCEEEECCCEEEECCCCCEEEEEEECCCEEEEECCCCCCHHHHHHHHHHHHCCCC
AAQGRLLLYQISLQALPVAIDLGLQIVKYGEEARVDLAAFTLDTPSAKPLRYAVRRAEKE
HHHCCEEEEEEEHHHHCCHHHCCHHHHHCCCHHCEEEEEEEECCCCCCHHHHHHHHHHHC
GARFAIIARDELAAHLPRLQEISESWLREKGHREKGFSVGRFDPPISPASTARSSCARRR
CCEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHC
LSPSPISGRRRIAPSCRST
CCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11342591; 12496209 [H]