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The map label for this gene is klcA [H]

Identifier: 148550680

GI number: 148550680

Start: 132608

End: 133042

Strand: Reverse

Name: klcA [H]

Synonym: Swit_5243

Alternate gene names: 148550680

Gene position: 133042-132608 (Counterclockwise)

Preceding gene: 148550681

Following gene: 148550679

Centisome position: 42.89

GC content: 64.6

Gene sequence:

>435_bases
ATGACAGACACTCTTCCCGCCATCGCCGATGCCCCCTGCAACGCCGCAACCCCCGTGCCCGACGCGCGGCGCGGCACATT
CCTCCCTGCGCTTTTTGGCCGCCGTCATTTCTTCAAGGGCGAGACGCTGGTCTATTCCTTCCTGCAGTGGCTCTCGGCCG
ATTATGGCGGCGGCTACTGGCAGTTCATGGAACAGGCGGGGCACCCGCTCTATCTCGTCCCCGCCGGACGACCGGCCTAT
CGCATCTGCTGCAACAGCAACCGCTATGAGGGCACCGTCTCCGCCGACGCTGCGGGCATCATCGCCACGCTCTTCGCCTT
CTCGCACCTCTCCTTCGAGGTCGAAGACGATCATATCGCCGAGGCCTTCCACCGCCTGCGCGACTATGCCGGCGATCATC
CCGAGGCCGCGAAAATCTTCGGCGCGATCGACTAG

Upstream 100 bases:

>100_bases
GCCGCCGCTCGCGCGGCGCTGTGAAGGGGAGGGGGAGCGGTGATGATGGTCCTGCCGCGAAAAGGAGCGTCCGGGCCGGT
CCCTTTGTCGGAGTTCCCGC

Downstream 100 bases:

>100_bases
GTCCGTTCCATGCGCGGGTCTGCCGTCAGACGCCAGGCCCCCCTGACCCCTGGAGGATTTCATGTTCAGCATTTTTGATA
TCGGCGCGGCGCCGGCCAAT

Product: antirestriction protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 144; Mature: 143

Protein sequence:

>144_residues
MTDTLPAIADAPCNAATPVPDARRGTFLPALFGRRHFFKGETLVYSFLQWLSADYGGGYWQFMEQAGHPLYLVPAGRPAY
RICCNSNRYEGTVSADAAGIIATLFAFSHLSFEVEDDHIAEAFHRLRDYAGDHPEAAKIFGAID

Sequences:

>Translated_144_residues
MTDTLPAIADAPCNAATPVPDARRGTFLPALFGRRHFFKGETLVYSFLQWLSADYGGGYWQFMEQAGHPLYLVPAGRPAY
RICCNSNRYEGTVSADAAGIIATLFAFSHLSFEVEDDHIAEAFHRLRDYAGDHPEAAKIFGAID
>Mature_143_residues
TDTLPAIADAPCNAATPVPDARRGTFLPALFGRRHFFKGETLVYSFLQWLSADYGGGYWQFMEQAGHPLYLVPAGRPAYR
ICCNSNRYEGTVSADAAGIIATLFAFSHLSFEVEDDHIAEAFHRLRDYAGDHPEAAKIFGAID

Specific function: Could be involved in overcoming restriction barriers during establishment after conjugative transfer [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the antirestriction protein family [H]

Homologues:

Organism=Escherichia coli, GI1786442, Length=135, Percent_Identity=34.0740740740741, Blast_Score=72, Evalue=2e-14,
Organism=Escherichia coli, GI1788996, Length=145, Percent_Identity=30.3448275862069, Blast_Score=67, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004914
- ProDom:   PD012854 [H]

Pfam domain/function: PF03230 Antirestrict [H]

EC number: NA

Molecular weight: Translated: 15875; Mature: 15744

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDTLPAIADAPCNAATPVPDARRGTFLPALFGRRHFFKGETLVYSFLQWLSADYGGGYW
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCHHCCCCHHHHHHHHHHHCCCCCCHHH
QFMEQAGHPLYLVPAGRPAYRICCNSNRYEGTVSADAAGIIATLFAFSHLSFEVEDDHIA
HHHHHCCCCEEEEECCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCEEECHHHHH
EAFHRLRDYAGDHPEAAKIFGAID
HHHHHHHHHCCCCCCHHHHHCCCC
>Mature Secondary Structure 
TDTLPAIADAPCNAATPVPDARRGTFLPALFGRRHFFKGETLVYSFLQWLSADYGGGYW
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCHHCCCCHHHHHHHHHHHCCCCCCHHH
QFMEQAGHPLYLVPAGRPAYRICCNSNRYEGTVSADAAGIIATLFAFSHLSFEVEDDHIA
HHHHHCCCCEEEEECCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCEEECHHHHH
EAFHRLRDYAGDHPEAAKIFGAID
HHHHHHHHHCCCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7773415 [H]