Definition Sphingomonas wittichii RW1 plasmid pSWIT01, complete sequence.
Accession NC_009507
Length 310,228

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The map label for this gene is insH1 [C]

Identifier: 148550563

GI number: 148550563

Start: 7623

End: 8996

Strand: Direct

Name: insH1 [C]

Synonym: Swit_5124

Alternate gene names: 148550563

Gene position: 7623-8996 (Clockwise)

Preceding gene: 148550560

Following gene: 148550565

Centisome position: 2.46

GC content: 64.41

Gene sequence:

>1374_bases
ATGATGGGCGAGCGGACGGTGGCGCAGGAAGCGCTGTTCTACAGCTTCAATCTGGAGCGGCATGTGCCGCCGGATCATCT
GCTACGCTCGATCGACCGGTTCGTGGACCTGTCGGATATCCGCGAGCACCTGCGGCCTTATTATAGCGAGACGGGTCGGC
CATCGATCGACCCAGAGTTGATGATCCGGATGCTGATCATCGGCTATTGCATGGGCATCCGGTCGGAGCGACGGCTGTGT
GACGAGGTGCATCTCAACCTCGCCTATCGCTGGTTCTGCCGGCTGGGGCTGGAGGGCGACGTGCCCGATCACTCGACGTT
CTCGAAGAACCGGCATGGACGCTTCCGCGACAGCGATCTGCTGCGGCAACTGTTCGAGATGACGGTGGCGCGCTGCATGG
CCGAAGGACTGGTCGGCGGCGAAGGCTTTGCGGTCGATGCGAGCCTGATCCGGGCGGATGTCCACCGGCAACGGTCGGTG
CCGGGTGAAGAGGGCTTGCCGCCGGAAGCGGTCGGTCGTGCGGTCGCTGAGTATCTGGACGTGCTGGACGATGCGGCGTT
CGGCGGTGCAACGCCGGTGACACCCAAGCGGATCAATCTCACCGATCCAGCCTCGCGCTGGACCGCCGCCACGCGCGAGG
CGGCCTTTTACGCCTACAGCACCAACTATCTGATCGACCTCGATCATGCGGTCATCGTCGATGTCGAGGCGACCACCTCG
GTGCGGCAGGCGGAGGTGACCGCCCAGCGCAGGATGATCGAGCGAACGCAGGATCGCTTCGGCATCTGGCCCGAGCGCCT
CGTGGCCGATACCGCCTATGGCTCTGCCCCCAACCTGGCCTGGCTGGTCGAAGAGATGAGCATCGAGCCGCATATTCCGG
TGTTCGACAAGTCCGCTCGGACTGACGGCAGCTTCGAGCGCGCCGACTTCGTCTATGACCATGATGACGACAGCTACATC
TGCCCGGTCGGCAACCGCCTACGGCGTTCCAACCGCAACTTCAGCACGCCCCGTGATCGCGTCGATAAGGACGGTTCGAT
CCGCTACCGCGCGCGTCAGCAGGACTGCCAGAGCTGTGCTCTGCGCCAGCGATGCACGCCCAACATGCCCGCTCGCAAGG
TCACCCGATCGATCCACGAAGGCGCGCGCGATCTGGCCCGCGACATCGCCACCACCGACGCGTATCTCGTCTCACGTCGC
CAGCGCAAGAAGGTCGAGATGCTGTTCGCCCACCTCAAGCGCATCCTGAAGCTGGATCGCTTGCGCCTGCGCGGCCCCAA
TGGCGCAAGAGACGAGTTCCACCTCGCCGCAGCGGCACAGAACCTCCGCAAAATGGCAAAGATCATCCCGATGGCCAGGC
CCGCACCGGCCTGA

Upstream 100 bases:

>100_bases
TAGCGCCGTTCCAGTTCTCAACCTATTTTGTTGAAAAACTTGCGCTTGAAGTGGCGCTGAAGGTCTGATTCACTCTGCAT
AGGCGAGCGGAGATGATCCG

Downstream 100 bases:

>100_bases
TCGGCAAAGGACGTCGCTCGCGTCCATCGCGAAGCGCAGTCAAACGGTCTCGCAGCTGACTTCTTCAACAGAATACAACC
ACATTCAGCCCTTCGTGGAT

Product: IS4 family transposase

Products: NA

Alternate protein names: Transposase IS4 Family Protein; ISBma2 Transposase; Transposase IS4 Family; IS4 Family Transposase; ISPsy22 Transposase Truncated; Transposase And Inactivated Derivative; IS5 Family Transposase N-Terminal Domain Protein; Transposase Is4 Family Protein; ISGsu3 Transposase; Transposase-Related Protein; ISMca1 Transposase; Transposase Mutator Type; IS5 Family Transposase N-Terminal Domain; Phage-Associated Cell Wall Hydrolase; Transposase Truncated; ISGsu2 Transposase; ISPsy6 Transposase; Transposase Fragment IS5 Family; ISCps6 Transposase; IS4 Transposase; Transposase For IS; Transposase For Insertion Sequence NGRIS-8b; ISPpu20 Transposase; ISSag8 Transposase; Transposase Fragment

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MMGERTVAQEALFYSFNLERHVPPDHLLRSIDRFVDLSDIREHLRPYYSETGRPSIDPELMIRMLIIGYCMGIRSERRLC
DEVHLNLAYRWFCRLGLEGDVPDHSTFSKNRHGRFRDSDLLRQLFEMTVARCMAEGLVGGEGFAVDASLIRADVHRQRSV
PGEEGLPPEAVGRAVAEYLDVLDDAAFGGATPVTPKRINLTDPASRWTAATREAAFYAYSTNYLIDLDHAVIVDVEATTS
VRQAEVTAQRRMIERTQDRFGIWPERLVADTAYGSAPNLAWLVEEMSIEPHIPVFDKSARTDGSFERADFVYDHDDDSYI
CPVGNRLRRSNRNFSTPRDRVDKDGSIRYRARQQDCQSCALRQRCTPNMPARKVTRSIHEGARDLARDIATTDAYLVSRR
QRKKVEMLFAHLKRILKLDRLRLRGPNGARDEFHLAAAAQNLRKMAKIIPMARPAPA

Sequences:

>Translated_457_residues
MMGERTVAQEALFYSFNLERHVPPDHLLRSIDRFVDLSDIREHLRPYYSETGRPSIDPELMIRMLIIGYCMGIRSERRLC
DEVHLNLAYRWFCRLGLEGDVPDHSTFSKNRHGRFRDSDLLRQLFEMTVARCMAEGLVGGEGFAVDASLIRADVHRQRSV
PGEEGLPPEAVGRAVAEYLDVLDDAAFGGATPVTPKRINLTDPASRWTAATREAAFYAYSTNYLIDLDHAVIVDVEATTS
VRQAEVTAQRRMIERTQDRFGIWPERLVADTAYGSAPNLAWLVEEMSIEPHIPVFDKSARTDGSFERADFVYDHDDDSYI
CPVGNRLRRSNRNFSTPRDRVDKDGSIRYRARQQDCQSCALRQRCTPNMPARKVTRSIHEGARDLARDIATTDAYLVSRR
QRKKVEMLFAHLKRILKLDRLRLRGPNGARDEFHLAAAAQNLRKMAKIIPMARPAPA
>Mature_457_residues
MMGERTVAQEALFYSFNLERHVPPDHLLRSIDRFVDLSDIREHLRPYYSETGRPSIDPELMIRMLIIGYCMGIRSERRLC
DEVHLNLAYRWFCRLGLEGDVPDHSTFSKNRHGRFRDSDLLRQLFEMTVARCMAEGLVGGEGFAVDASLIRADVHRQRSV
PGEEGLPPEAVGRAVAEYLDVLDDAAFGGATPVTPKRINLTDPASRWTAATREAAFYAYSTNYLIDLDHAVIVDVEATTS
VRQAEVTAQRRMIERTQDRFGIWPERLVADTAYGSAPNLAWLVEEMSIEPHIPVFDKSARTDGSFERADFVYDHDDDSYI
CPVGNRLRRSNRNFSTPRDRVDKDGSIRYRARQQDCQSCALRQRCTPNMPARKVTRSIHEGARDLARDIATTDAYLVSRR
QRKKVEMLFAHLKRILKLDRLRLRGPNGARDEFHLAAAAQNLRKMAKIIPMARPAPA

Specific function: Involved in the transposition of the insertion sequence is5 [C]

COG id: COG3666

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 52132; Mature: 52132

Theoretical pI: Translated: 8.39; Mature: 8.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMGERTVAQEALFYSFNLERHVPPDHLLRSIDRFVDLSDIREHLRPYYSETGRPSIDPEL
CCCCHHHHHHHHHHEECCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCHHH
MIRMLIIGYCMGIRSERRLCDEVHLNLAYRWFCRLGLEGDVPDHSTFSKNRHGRFRDSDL
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHH
LRQLFEMTVARCMAEGLVGGEGFAVDASLIRADVHRQRSVPGEEGLPPEAVGRAVAEYLD
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
VLDDAAFGGATPVTPKRINLTDPASRWTAATREAAFYAYSTNYLIDLDHAVIVDVEATTS
HHHHHCCCCCCCCCCCEECCCCCHHHHHHHHHHHEEEEEECCEEEEECCEEEEEECCCCH
VRQAEVTAQRRMIERTQDRFGIWPERLVADTAYGSAPNLAWLVEEMSIEPHIPVFDKSAR
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCC
TDGSFERADFVYDHDDDSYICPVGNRLRRSNRNFSTPRDRVDKDGSIRYRARQQDCQSCA
CCCCCCCCCCEEECCCCCEECCCCHHHHHCCCCCCCCHHHCCCCCCEEEHHHHHHHHHHH
LRQRCTPNMPARKVTRSIHEGARDLARDIATTDAYLVSRRQRKKVEMLFAHLKRILKLDR
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRLRGPNGARDEFHLAAAAQNLRKMAKIIPMARPAPA
HHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MMGERTVAQEALFYSFNLERHVPPDHLLRSIDRFVDLSDIREHLRPYYSETGRPSIDPEL
CCCCHHHHHHHHHHEECCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCHHH
MIRMLIIGYCMGIRSERRLCDEVHLNLAYRWFCRLGLEGDVPDHSTFSKNRHGRFRDSDL
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHH
LRQLFEMTVARCMAEGLVGGEGFAVDASLIRADVHRQRSVPGEEGLPPEAVGRAVAEYLD
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
VLDDAAFGGATPVTPKRINLTDPASRWTAATREAAFYAYSTNYLIDLDHAVIVDVEATTS
HHHHHCCCCCCCCCCCEECCCCCHHHHHHHHHHHEEEEEECCEEEEECCEEEEEECCCCH
VRQAEVTAQRRMIERTQDRFGIWPERLVADTAYGSAPNLAWLVEEMSIEPHIPVFDKSAR
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCC
TDGSFERADFVYDHDDDSYICPVGNRLRRSNRNFSTPRDRVDKDGSIRYRARQQDCQSCA
CCCCCCCCCCEEECCCCCEECCCCHHHHHCCCCCCCCHHHCCCCCCEEEHHHHHHHHHHH
LRQRCTPNMPARKVTRSIHEGARDLARDIATTDAYLVSRRQRKKVEMLFAHLKRILKLDR
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRLRGPNGARDEFHLAAAAQNLRKMAKIIPMARPAPA
HHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA