| Definition | Clostridium botulinum A str. ATCC 3502, complete genome. |
|---|---|
| Accession | NC_009495 |
| Length | 3,886,916 |
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The map label for this gene is nrdE [H]
Identifier: 148380774
GI number: 148380774
Start: 2981019
End: 2983310
Strand: Reverse
Name: nrdE [H]
Synonym: CBO2814
Alternate gene names: 148380774
Gene position: 2983310-2981019 (Counterclockwise)
Preceding gene: 148380777
Following gene: 148380773
Centisome position: 76.75
GC content: 31.28
Gene sequence:
>2292_bases ATGAATATTAAAATAAAAAAGAGAGATGGACAATATGAACCACTACAAGTAGAAAAAACAAAAAAAATGGTTAAGCTAGC CTGTGAGGGTATAGAAGGATGTGACCCTCTTGAACTAGAGTTGGATTCCAGAATACAATTTAGAGATGGTATGACTACTA AGGAAATTCAAAGAACTTTGATACAAACTGCCATCGAAAAAGTTATTCAAAATAGCAAAGATAACAATGGAAATAATATA AAAAAAACTAATGCTAATTGGCAGTATGTTGCTGCAAGACTTTTATGTTTTGATTTATACAAGGAAGCTAAAATCAGCCG ACACTATAATAGCTTTGGTTATGGTGATTATTATCAATTGGTAAAGAAATTAGTTAAAATTAAACTTTATGGAGAGTATT TAATCCAAAATTATTCTGATGAAGAAATCAAAGAATTAGCAAAGTATATAGTACCAGAAAGAGATGAATTATTTAATTAT GAAGGATTAAAATTATTAAATGATAGATATTTAATTAAGGGGAATAATGGAGAAATATTAGAGCTTCCTCAAGAACGCTT TATGACAATAGCTATGCATTTAGCTATTCCTGAGGGGGATAAAAAAGTTTTTTATGCAAAAAAGTTTTATGATCTTTTAA GTGAGTTAAAAGTTACAGTGGCCACTCCTACTTTAGGAAATGCAGGTACACCTTTTTATCAGTTAAGTAGCTGTTTTATA TCTGTTGTAGGGGATAATTTATGGTCAATTTATGATGTTAATCAAAAGTTTGCACAAGTCTCAAAACATGGTGGAGCACT AGGCATTTATACTGGAAAAATAAGAGCGTTAAACAGTGAGATAAGAGGGCATAAAAATGCATCAGGGGGGGTTGTACCCT GGATAAGACTTTATAATGATACAGCTGTAGCTGTAGACCAGCTTGGCAAGAGAAAAGGTGGAGCAGCTATAACTTTAGAT ATATGGCATAAAGATATATTTGATTTTTTAGATCTTAAAACTAATAATGGAGATGATAGAAGAAAGGCTCATGATATTTT TCCTTCTGTAAGCATTCCAGACTTATTTATGAAAAGATTAGAGAAAAGAGAAAGTTGGTCTTTATTTGATCCTTATATTG TAGAGAAGATAATGGGCTACAAGTTGGAGGATTATTTTGACGATGAAGATCGCAAGGAATTTACAAATAAGTACTTAGAA TGTGAAAGAAATACTAATATTCCAAGAGATACAGTACCGACTCTTGATATTATGAAAAAATTAATGAAAAGTGCAGTAGA GACAGGTACTCCTTTTATTTTTTTTAGGGATACTGTAAATAAAGCAAATCCTAATAAACATAAAGGTATGATATATTCAT CAAATCTTTGCCATGAAATAGCTCAAAATATGAGTGAAAGTCGACTTCTAGAAGAGGAAATAATTGATGGCAATGGTTAC TCAGAGATTGTTCAAAGAGTTAAGGCTGGAGATATGGTAACCTGTAATCTTAATTCAATAAATCTTAGCAAAGTGAAAAA AGAGGAATTTAATGAGTGTATCCCTTTTCAAATAAGGATGCTAGATAATGTTATATCTCTTAATAAGCTTCCTGTTAAAG AGGCAAAAGTTACCAGTGACAAGTATAGAGCTATTGGGTTAGGAACAAGTGGATATCATCATTTCCTTGCAAATAACAAG ATAAGATGGGAAAGTGATGAACATATTAAGGTAGCGGATGAAATCTATGAAGAGATAGCCTATATAGCTATAAAATCTTC CATGGAATTAGCTAAGGAAAAAGGCAGTTATCCAGCTTTTAAGGATTCAGAATGGGAAACAGGAAAATATTTTGAAAGAA GAGGTTACAATTCTGAAAGATGGAAGAAGCTACAAAGTAATATCAAAAAATATGGTATGAGAAATGGTTATATTACTGCT ATTGCCCCTACAGGAAGTACTTCTAATATAGCTAATACAACAGCAGGAATAGACCCTGTATTTAAAAAGTTTTTTATGGA AGAAAAGAAGGGTAGCTTTACACCAAAAACAGCACCAGACTTAAATGAAGAAAATTTTTGGTATTATAAAGAAGCACATA CTATAGACCAACAATGGAGCATTAAGGCTTGTGCCGTAAGACAAAAGCATATAGATCAGGCTCAATCCTTTAATTTATAT ATAACTCCAGAAATAAAAGCTAAAGAAATTCTTAATATGTATATAGAATCTTGGAAGCAAGGAGTTAAAACTATCTATTA TGTAAGAAATAAATCTTTAGAAATGGATGAATGTACAAGTTGCTCAAGCTAA
Upstream 100 bases:
>100_bases TTGATTTTAAAAATAATAACTTATATAATTTATTTAACAACGGTTATTTTATAATATTTTTTATTGATTAAATAAATTTT TAGAAAGTAAGGTTTGAGCA
Downstream 100 bases:
>100_bases ATGTATATTTAAAGTTATAACAAGAGAAAGATTGTAATTTTAAAGCAGATGAAGTGCTAGGGTTAAAGTATATACTTAAT ATAATACCTAAATAGGTAAA
Product: ribonucleotide-diphosphate reductase subunit alpha
Products: NA
Alternate protein names: Ribonucleotide reductase [H]
Number of amino acids: Translated: 763; Mature: 763
Protein sequence:
>763_residues MNIKIKKRDGQYEPLQVEKTKKMVKLACEGIEGCDPLELELDSRIQFRDGMTTKEIQRTLIQTAIEKVIQNSKDNNGNNI KKTNANWQYVAARLLCFDLYKEAKISRHYNSFGYGDYYQLVKKLVKIKLYGEYLIQNYSDEEIKELAKYIVPERDELFNY EGLKLLNDRYLIKGNNGEILELPQERFMTIAMHLAIPEGDKKVFYAKKFYDLLSELKVTVATPTLGNAGTPFYQLSSCFI SVVGDNLWSIYDVNQKFAQVSKHGGALGIYTGKIRALNSEIRGHKNASGGVVPWIRLYNDTAVAVDQLGKRKGGAAITLD IWHKDIFDFLDLKTNNGDDRRKAHDIFPSVSIPDLFMKRLEKRESWSLFDPYIVEKIMGYKLEDYFDDEDRKEFTNKYLE CERNTNIPRDTVPTLDIMKKLMKSAVETGTPFIFFRDTVNKANPNKHKGMIYSSNLCHEIAQNMSESRLLEEEIIDGNGY SEIVQRVKAGDMVTCNLNSINLSKVKKEEFNECIPFQIRMLDNVISLNKLPVKEAKVTSDKYRAIGLGTSGYHHFLANNK IRWESDEHIKVADEIYEEIAYIAIKSSMELAKEKGSYPAFKDSEWETGKYFERRGYNSERWKKLQSNIKKYGMRNGYITA IAPTGSTSNIANTTAGIDPVFKKFFMEEKKGSFTPKTAPDLNEENFWYYKEAHTIDQQWSIKACAVRQKHIDQAQSFNLY ITPEIKAKEILNMYIESWKQGVKTIYYVRNKSLEMDECTSCSS
Sequences:
>Translated_763_residues MNIKIKKRDGQYEPLQVEKTKKMVKLACEGIEGCDPLELELDSRIQFRDGMTTKEIQRTLIQTAIEKVIQNSKDNNGNNI KKTNANWQYVAARLLCFDLYKEAKISRHYNSFGYGDYYQLVKKLVKIKLYGEYLIQNYSDEEIKELAKYIVPERDELFNY EGLKLLNDRYLIKGNNGEILELPQERFMTIAMHLAIPEGDKKVFYAKKFYDLLSELKVTVATPTLGNAGTPFYQLSSCFI SVVGDNLWSIYDVNQKFAQVSKHGGALGIYTGKIRALNSEIRGHKNASGGVVPWIRLYNDTAVAVDQLGKRKGGAAITLD IWHKDIFDFLDLKTNNGDDRRKAHDIFPSVSIPDLFMKRLEKRESWSLFDPYIVEKIMGYKLEDYFDDEDRKEFTNKYLE CERNTNIPRDTVPTLDIMKKLMKSAVETGTPFIFFRDTVNKANPNKHKGMIYSSNLCHEIAQNMSESRLLEEEIIDGNGY SEIVQRVKAGDMVTCNLNSINLSKVKKEEFNECIPFQIRMLDNVISLNKLPVKEAKVTSDKYRAIGLGTSGYHHFLANNK IRWESDEHIKVADEIYEEIAYIAIKSSMELAKEKGSYPAFKDSEWETGKYFERRGYNSERWKKLQSNIKKYGMRNGYITA IAPTGSTSNIANTTAGIDPVFKKFFMEEKKGSFTPKTAPDLNEENFWYYKEAHTIDQQWSIKACAVRQKHIDQAQSFNLY ITPEIKAKEILNMYIESWKQGVKTIYYVRNKSLEMDECTSCSS >Mature_763_residues MNIKIKKRDGQYEPLQVEKTKKMVKLACEGIEGCDPLELELDSRIQFRDGMTTKEIQRTLIQTAIEKVIQNSKDNNGNNI KKTNANWQYVAARLLCFDLYKEAKISRHYNSFGYGDYYQLVKKLVKIKLYGEYLIQNYSDEEIKELAKYIVPERDELFNY EGLKLLNDRYLIKGNNGEILELPQERFMTIAMHLAIPEGDKKVFYAKKFYDLLSELKVTVATPTLGNAGTPFYQLSSCFI SVVGDNLWSIYDVNQKFAQVSKHGGALGIYTGKIRALNSEIRGHKNASGGVVPWIRLYNDTAVAVDQLGKRKGGAAITLD IWHKDIFDFLDLKTNNGDDRRKAHDIFPSVSIPDLFMKRLEKRESWSLFDPYIVEKIMGYKLEDYFDDEDRKEFTNKYLE CERNTNIPRDTVPTLDIMKKLMKSAVETGTPFIFFRDTVNKANPNKHKGMIYSSNLCHEIAQNMSESRLLEEEIIDGNGY SEIVQRVKAGDMVTCNLNSINLSKVKKEEFNECIPFQIRMLDNVISLNKLPVKEAKVTSDKYRAIGLGTSGYHHFLANNK IRWESDEHIKVADEIYEEIAYIAIKSSMELAKEKGSYPAFKDSEWETGKYFERRGYNSERWKKLQSNIKKYGMRNGYITA IAPTGSTSNIANTTAGIDPVFKKFFMEEKKGSFTPKTAPDLNEENFWYYKEAHTIDQQWSIKACAVRQKHIDQAQSFNLY ITPEIKAKEILNMYIESWKQGVKTIYYVRNKSLEMDECTSCSS
Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]
COG id: COG0209
COG function: function code F; Ribonucleotide reductase, alpha subunit
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 ATP-cone domain [H]
Homologues:
Organism=Homo sapiens, GI4506749, Length=806, Percent_Identity=29.6526054590571, Blast_Score=308, Evalue=2e-83, Organism=Escherichia coli, GI2367133, Length=768, Percent_Identity=27.8645833333333, Blast_Score=303, Evalue=4e-83, Organism=Escherichia coli, GI1789030, Length=696, Percent_Identity=28.0172413793103, Blast_Score=261, Evalue=9e-71, Organism=Caenorhabditis elegans, GI17555242, Length=796, Percent_Identity=29.3969849246231, Blast_Score=296, Evalue=2e-80, Organism=Saccharomyces cerevisiae, GI6320914, Length=801, Percent_Identity=28.8389513108614, Blast_Score=301, Evalue=3e-82, Organism=Saccharomyces cerevisiae, GI6322123, Length=804, Percent_Identity=29.6019900497512, Blast_Score=292, Evalue=2e-79, Organism=Drosophila melanogaster, GI17352481, Length=796, Percent_Identity=29.3969849246231, Blast_Score=300, Evalue=2e-81,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR005144 - InterPro: IPR013346 - InterPro: IPR013509 - InterPro: IPR000788 - InterPro: IPR008926 [H]
Pfam domain/function: PF03477 ATP-cone; PF02867 Ribonuc_red_lgC; PF00317 Ribonuc_red_lgN [H]
EC number: =1.17.4.1 [H]
Molecular weight: Translated: 88044; Mature: 88044
Theoretical pI: Translated: 8.38; Mature: 8.38
Prosite motif: PS51161 ATP_CONE ; PS00089 RIBORED_LARGE
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.4 %Cys (Translated Protein) 2.4 %Met (Translated Protein) 3.8 %Cys+Met (Translated Protein) 1.4 %Cys (Mature Protein) 2.4 %Met (Mature Protein) 3.8 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MNIKIKKRDGQYEPLQVEKTKKMVKLACEGIEGCDPLELELDSRIQFRDGMTTKEIQRTL CCEEEECCCCCCCCEEHHHHHHHHHHHHCCCCCCCCCEEECCCCEEECCCCCHHHHHHHH IQTAIEKVIQNSKDNNGNNIKKTNANWQYVAARLLCFDLYKEAKISRHYNSFGYGDYYQL HHHHHHHHHHCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH VKKLVKIKLYGEYLIQNYSDEEIKELAKYIVPERDELFNYEGLKLLNDRYLIKGNNGEIL HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHCCCCCEEEECCEEEEECCCCCEE ELPQERFMTIAMHLAIPEGDKKVFYAKKFYDLLSELKVTVATPTLGNAGTPFYQLSSCFI ECCHHHHHEEEEEEECCCCCCEEEHHHHHHHHHHHCEEEEECCCCCCCCCCHHHHHHHHH SVVGDNLWSIYDVNQKFAQVSKHGGALGIYTGKIRALNSEIRGHKNASGGVVPWIRLYND HHHCCCCEEEEEHHHHHHHHHHCCCEEEEEECHHEEHHHHHCCCCCCCCCCCEEEEEECC TAVAVDQLGKRKGGAAITLDIWHKDIFDFLDLKTNNGDDRRKAHDIFPSVSIPDLFMKRL CHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCHHHHHHHH EKRESWSLFDPYIVEKIMGYKLEDYFDDEDRKEFTNKYLECERNTNIPRDTVPTLDIMKK HHCCCCCCCCHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH LMKSAVETGTPFIFFRDTVNKANPNKHKGMIYSSNLCHEIAQNMSESRLLEEEIIDGNGY HHHHHHHCCCCEEEEECCCCCCCCCCCCCEEEEHHHHHHHHHCHHHHHHHHHHHCCCCCH SEIVQRVKAGDMVTCNLNSINLSKVKKEEFNECIPFQIRMLDNVISLNKLPVKEAKVTSD HHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHCCCC KYRAIGLGTSGYHHFLANNKIRWESDEHIKVADEIYEEIAYIAIKSSMELAKEKGSYPAF CEEEEECCCCCHHHHHCCCEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC KDSEWETGKYFERRGYNSERWKKLQSNIKKYGMRNGYITAIAPTGSTSNIANTTAGIDPV CCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCHH FKKFFMEEKKGSFTPKTAPDLNEENFWYYKEAHTIDQQWSIKACAVRQKHIDQAQSFNLY HHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCEE ITPEIKAKEILNMYIESWKQGVKTIYYVRNKSLEMDECTSCSS ECCCCCHHHHHHHHHHHHHHHHHEEEEEECCCCCHHHHCCCCC >Mature Secondary Structure MNIKIKKRDGQYEPLQVEKTKKMVKLACEGIEGCDPLELELDSRIQFRDGMTTKEIQRTL CCEEEECCCCCCCCEEHHHHHHHHHHHHCCCCCCCCCEEECCCCEEECCCCCHHHHHHHH IQTAIEKVIQNSKDNNGNNIKKTNANWQYVAARLLCFDLYKEAKISRHYNSFGYGDYYQL HHHHHHHHHHCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH VKKLVKIKLYGEYLIQNYSDEEIKELAKYIVPERDELFNYEGLKLLNDRYLIKGNNGEIL HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHCCCCCEEEECCEEEEECCCCCEE ELPQERFMTIAMHLAIPEGDKKVFYAKKFYDLLSELKVTVATPTLGNAGTPFYQLSSCFI ECCHHHHHEEEEEEECCCCCCEEEHHHHHHHHHHHCEEEEECCCCCCCCCCHHHHHHHHH SVVGDNLWSIYDVNQKFAQVSKHGGALGIYTGKIRALNSEIRGHKNASGGVVPWIRLYND HHHCCCCEEEEEHHHHHHHHHHCCCEEEEEECHHEEHHHHHCCCCCCCCCCCEEEEEECC TAVAVDQLGKRKGGAAITLDIWHKDIFDFLDLKTNNGDDRRKAHDIFPSVSIPDLFMKRL CHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCHHHHHHHH EKRESWSLFDPYIVEKIMGYKLEDYFDDEDRKEFTNKYLECERNTNIPRDTVPTLDIMKK HHCCCCCCCCHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH LMKSAVETGTPFIFFRDTVNKANPNKHKGMIYSSNLCHEIAQNMSESRLLEEEIIDGNGY HHHHHHHCCCCEEEEECCCCCCCCCCCCCEEEEHHHHHHHHHCHHHHHHHHHHHCCCCCH SEIVQRVKAGDMVTCNLNSINLSKVKKEEFNECIPFQIRMLDNVISLNKLPVKEAKVTSD HHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHCCCC KYRAIGLGTSGYHHFLANNKIRWESDEHIKVADEIYEEIAYIAIKSSMELAKEKGSYPAF CEEEEECCCCCHHHHHCCCEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC KDSEWETGKYFERRGYNSERWKKLQSNIKKYGMRNGYITAIAPTGSTSNIANTTAGIDPV CCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCHH FKKFFMEEKKGSFTPKTAPDLNEENFWYYKEAHTIDQQWSIKACAVRQKHIDQAQSFNLY HHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCEE ITPEIKAKEILNMYIESWKQGVKTIYYVRNKSLEMDECTSCSS ECCCCCHHHHHHHHHHHHHHHHHEEEEEECCCCCHHHHCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 9537320 [H]