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The map label for this gene is nrdF [H]

Identifier: 148380773

GI number: 148380773

Start: 2979795

End: 2980823

Strand: Reverse

Name: nrdF [H]

Synonym: CBO2813

Alternate gene names: 148380773

Gene position: 2980823-2979795 (Counterclockwise)

Preceding gene: 148380774

Following gene: 148380772

Centisome position: 76.69

GC content: 28.77

Gene sequence:

>1029_bases
ATGCTTAAAAAAATGATATTTAACGAAAAGGGGCAACGGGGAACGGAATCAATGATAAATGGAAACACTACTAACTTAAG
AGAGTGGAATAGAATAAAATATAGCTGGGCTAGCGATTTTTATAGAACAATGCTTAATAATTTTTGGATACCAGAAGAAA
TTTCTTTAAATGAGGATATAAAACAATTTCCGTACTTAACAGATGGAGAAAGAAATGCCTTTGATAAGATTATTTCTTTT
TTAAATTTTCTTGATTCCGTGCAAAGTGAAAATCTTCCTAATATATCGAGATATATTACTGCTGCAGAGGTTTCTTCTCT
TTTAAACATTCAAACCTTCCAAGAAGAAATACATGCACAAAGTTATTCATACATACTTGATACAGTTACGAATCCAATTA
CTAGAGATAAAATATATGATCAATGGAGAGAAGATGAACATCTCTTAGAAAGAAATAAATTTATAGCAGGTATTTATGAA
AAGTTTAATAAAGAACCTGAAATCCATAATTTTTTAAGAGCAATCATGGCAAATTATATATTAGAGGGAATATACTTTTA
TTCCGGTTTTAGCTTTTTTTATACCTTAGCTAGGCAGGGTAAAATGACTGCCACTAGTACAATTTTTAAATATATAAATA
GGGACGAAGTTACTCATCTTGTACTTTTTCAGAATATTATTAAAGAACTAAAAAATGAAAATTTGCATATTTTTACAGAA
GAATTAGAAGAAGAATTTAGGCAGATGATGAGAATGGGAGTAGAGCATGAAATACAATGGGGACAATATGTTACAAACAA
TGAAATATTAGGTCTTAATGATGAATTAATAGAAAAATATATTAAATATCTTTCTAATTTAAGATTAGTAGCTATAGGGT
TAAAACCCTTATATCCAGAAATTAACAAACATCCTATGGAGTGGATCGATAGCTTTTCAAAACTCAATAGTACAAAGACT
GATTTTTTTGAAGCAAAGGTTACGAACTATACAAAAGCAGCAGCTTTTGATTTTGATGATCTTGATTAA

Upstream 100 bases:

>100_bases
GTAAATAGGATAATTTATTAATTATAAATATGTTAAGTATACTTTTAAAACAATAACTATATTTACAAAATATGTGTTAA
AGAAAGGAGAATTATAGTTA

Downstream 100 bases:

>100_bases
TTAAGATTATAAATAAAGTTACAAAAATAGCCTAACAAAGTGCTAAAATACATTTTGTTAGGCTATTTTCTTATAATATT
TATTATCTTTAGGTGAAGTT

Product: ribonucleoside-diphosphate reductase, beta subunit

Products: NA

Alternate protein names: Ribonucleotide reductase small subunit [H]

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MLKKMIFNEKGQRGTESMINGNTTNLREWNRIKYSWASDFYRTMLNNFWIPEEISLNEDIKQFPYLTDGERNAFDKIISF
LNFLDSVQSENLPNISRYITAAEVSSLLNIQTFQEEIHAQSYSYILDTVTNPITRDKIYDQWREDEHLLERNKFIAGIYE
KFNKEPEIHNFLRAIMANYILEGIYFYSGFSFFYTLARQGKMTATSTIFKYINRDEVTHLVLFQNIIKELKNENLHIFTE
ELEEEFRQMMRMGVEHEIQWGQYVTNNEILGLNDELIEKYIKYLSNLRLVAIGLKPLYPEINKHPMEWIDSFSKLNSTKT
DFFEAKVTNYTKAAAFDFDDLD

Sequences:

>Translated_342_residues
MLKKMIFNEKGQRGTESMINGNTTNLREWNRIKYSWASDFYRTMLNNFWIPEEISLNEDIKQFPYLTDGERNAFDKIISF
LNFLDSVQSENLPNISRYITAAEVSSLLNIQTFQEEIHAQSYSYILDTVTNPITRDKIYDQWREDEHLLERNKFIAGIYE
KFNKEPEIHNFLRAIMANYILEGIYFYSGFSFFYTLARQGKMTATSTIFKYINRDEVTHLVLFQNIIKELKNENLHIFTE
ELEEEFRQMMRMGVEHEIQWGQYVTNNEILGLNDELIEKYIKYLSNLRLVAIGLKPLYPEINKHPMEWIDSFSKLNSTKT
DFFEAKVTNYTKAAAFDFDDLD
>Mature_342_residues
MLKKMIFNEKGQRGTESMINGNTTNLREWNRIKYSWASDFYRTMLNNFWIPEEISLNEDIKQFPYLTDGERNAFDKIISF
LNFLDSVQSENLPNISRYITAAEVSSLLNIQTFQEEIHAQSYSYILDTVTNPITRDKIYDQWREDEHLLERNKFIAGIYE
KFNKEPEIHNFLRAIMANYILEGIYFYSGFSFFYTLARQGKMTATSTIFKYINRDEVTHLVLFQNIIKELKNENLHIFTE
ELEEEFRQMMRMGVEHEIQWGQYVTNNEILGLNDELIEKYIKYLSNLRLVAIGLKPLYPEINKHPMEWIDSFSKLNSTKT
DFFEAKVTNYTKAAAFDFDDLD

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0208

COG function: function code F; Ribonucleotide reductase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase small chain family [H]

Homologues:

Organism=Homo sapiens, GI42544136, Length=313, Percent_Identity=22.3642172523962, Blast_Score=82, Evalue=9e-16,
Organism=Homo sapiens, GI4557845, Length=339, Percent_Identity=23.0088495575221, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI289177074, Length=313, Percent_Identity=22.3642172523962, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI260064013, Length=339, Percent_Identity=23.0088495575221, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI289177076, Length=293, Percent_Identity=21.8430034129693, Blast_Score=73, Evalue=4e-13,
Organism=Escherichia coli, GI1788567, Length=366, Percent_Identity=22.9508196721311, Blast_Score=111, Evalue=8e-26,
Organism=Escherichia coli, GI1789031, Length=322, Percent_Identity=27.0186335403727, Blast_Score=87, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17551968, Length=303, Percent_Identity=22.7722772277228, Blast_Score=85, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6322434, Length=311, Percent_Identity=27.0096463022508, Blast_Score=88, Evalue=2e-18,
Organism=Drosophila melanogaster, GI18079279, Length=308, Percent_Identity=24.3506493506494, Blast_Score=91, Evalue=8e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009078
- InterPro:   IPR012348
- InterPro:   IPR000358 [H]

Pfam domain/function: PF00268 Ribonuc_red_sm [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 40673; Mature: 40673

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKKMIFNEKGQRGTESMINGNTTNLREWNRIKYSWASDFYRTMLNNFWIPEEISLNEDI
CCHHHHHCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHH
KQFPYLTDGERNAFDKIISFLNFLDSVQSENLPNISRYITAAEVSSLLNIQTFQEEIHAQ
HHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
SYSYILDTVTNPITRDKIYDQWREDEHLLERNKFIAGIYEKFNKEPEIHNFLRAIMANYI
HHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
LEGIYFYSGFSFFYTLARQGKMTATSTIFKYINRDEVTHLVLFQNIIKELKNENLHIFTE
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHH
ELEEEFRQMMRMGVEHEIQWGQYVTNNEILGLNDELIEKYIKYLSNLRLVAIGLKPLYPE
HHHHHHHHHHHCCCHHCCCCCCEECCCCEECCCHHHHHHHHHHHCCCEEEEECCCCCCCC
INKHPMEWIDSFSKLNSTKTDFFEAKVTNYTKAAAFDFDDLD
CCCCHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHCCCCCCCC
>Mature Secondary Structure
MLKKMIFNEKGQRGTESMINGNTTNLREWNRIKYSWASDFYRTMLNNFWIPEEISLNEDI
CCHHHHHCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHH
KQFPYLTDGERNAFDKIISFLNFLDSVQSENLPNISRYITAAEVSSLLNIQTFQEEIHAQ
HHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
SYSYILDTVTNPITRDKIYDQWREDEHLLERNKFIAGIYEKFNKEPEIHNFLRAIMANYI
HHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
LEGIYFYSGFSFFYTLARQGKMTATSTIFKYINRDEVTHLVLFQNIIKELKNENLHIFTE
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHH
ELEEEFRQMMRMGVEHEIQWGQYVTNNEILGLNDELIEKYIKYLSNLRLVAIGLKPLYPE
HHHHHHHHHHHCCCHHCCCCCCEECCCCEECCCHHHHHHHHHHHCCCEEEEECCCCCCCC
INKHPMEWIDSFSKLNSTKTDFFEAKVTNYTKAAAFDFDDLD
CCCCHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9665876 [H]