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The map label for this gene is lutR [H]

Identifier: 148379489

GI number: 148379489

Start: 1654493

End: 1655203

Strand: Direct

Name: lutR [H]

Synonym: CBO1520

Alternate gene names: 148379489

Gene position: 1654493-1655203 (Clockwise)

Preceding gene: 148379487

Following gene: 148379491

Centisome position: 42.57

GC content: 27.71

Gene sequence:

>711_bases
ATGTTTAGTCCTATAAAAAACACGAGAGTTTATGAAAAAGCTATAGAACAAATTAAAGATATGATTGTTGAAGGTACTTT
TAAAAAGGGAGATAAACTTCCTTCTGAGAGGGAAATGGCAGAAAGCCTACAAATTAGTAGAACTTCTATAAGAGAAGCCC
TTAGAGAGTTAGAAATCATGGGACTTATAGAATCAAGACAAGGAGACGGAAACTTTGTAAAAAGTAGTTTTGAGAATAAC
TTATTAAAACCTTTGTCTACTATATTTTTATTAAAGGAAAGTAATCCAGATGAAATATTAGAACTTAGGCAAATTATTGA
AAGAGGATCTGTTGTATTAGCTGCTGAAAGAATCACTGATGAGGAACTAGAAGAAATGGAACTTCTTTTTCAAGATTCTT
CAAAATTAAATTTTAAAAATCAATTAGTAGATATAGATATAGCTTTTCATTATAAGATAGCTCAAGCTTCTAAAAATTTT
CTTATATTAAGTATATTAAATGCTATATCATTTTTAATTGAAGCTTTCATTAAAGACATAAGAAAAAATGTAATAACAAA
AAAAGAAAATACGGATATGCTTATAAAACAACATAGAGATATTTTGTCAGCATTAAAAGAGCATGATCCAATGGCTGCTG
AAGAAGCTATGTTATCTCACTTACAATATGTTAATAGCCAAATGAAAAAAGAAATAGAAATCACTACTTAA

Upstream 100 bases:

>100_bases
GTATAACAATCAGGGTTAACTATATGAGAATCTTAATATGCAAGATTTACATTTATAAAGACCTCATTATATTTTTACTT
TTAAGAAGGGATGAATAATT

Downstream 100 bases:

>100_bases
AGTACTATGTATTATATAGTGCTTTTTTCATTTTAAAGAAAAAAGTCATGGGAGATAGGTATTAGATATTTTATTTTTCA
TAAAGATATATTAAGTGAAA

Product: GntR family transcriptional regulator

Products: NA

Alternate protein names: L-lactate utilization operon repressor [H]

Number of amino acids: Translated: 236; Mature: 236

Protein sequence:

>236_residues
MFSPIKNTRVYEKAIEQIKDMIVEGTFKKGDKLPSEREMAESLQISRTSIREALRELEIMGLIESRQGDGNFVKSSFENN
LLKPLSTIFLLKESNPDEILELRQIIERGSVVLAAERITDEELEEMELLFQDSSKLNFKNQLVDIDIAFHYKIAQASKNF
LILSILNAISFLIEAFIKDIRKNVITKKENTDMLIKQHRDILSALKEHDPMAAEEAMLSHLQYVNSQMKKEIEITT

Sequences:

>Translated_236_residues
MFSPIKNTRVYEKAIEQIKDMIVEGTFKKGDKLPSEREMAESLQISRTSIREALRELEIMGLIESRQGDGNFVKSSFENN
LLKPLSTIFLLKESNPDEILELRQIIERGSVVLAAERITDEELEEMELLFQDSSKLNFKNQLVDIDIAFHYKIAQASKNF
LILSILNAISFLIEAFIKDIRKNVITKKENTDMLIKQHRDILSALKEHDPMAAEEAMLSHLQYVNSQMKKEIEITT
>Mature_236_residues
MFSPIKNTRVYEKAIEQIKDMIVEGTFKKGDKLPSEREMAESLQISRTSIREALRELEIMGLIESRQGDGNFVKSSFENN
LLKPLSTIFLLKESNPDEILELRQIIERGSVVLAAERITDEELEEMELLFQDSSKLNFKNQLVDIDIAFHYKIAQASKNF
LILSILNAISFLIEAFIKDIRKNVITKKENTDMLIKQHRDILSALKEHDPMAAEEAMLSHLQYVNSQMKKEIEITT

Specific function: Negatively regulates the transcription of the lutABC operon, which is required for L-lactate utilization. LutR activity is regulated by lactate, since presence of L-lactate, that probably binds to lutR, leads to derepression of the operon. Also appears to

COG id: COG2186

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790032, Length=231, Percent_Identity=31.1688311688312, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1786303, Length=232, Percent_Identity=30.1724137931034, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1790780, Length=248, Percent_Identity=30.241935483871, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI87082208, Length=222, Percent_Identity=29.7297297297297, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1789621, Length=230, Percent_Identity=28.695652173913, Blast_Score=85, Evalue=4e-18,
Organism=Escherichia coli, GI1789353, Length=221, Percent_Identity=27.6018099547511, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI48994955, Length=221, Percent_Identity=27.6018099547511, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI48994961, Length=209, Percent_Identity=25.3588516746411, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 27204; Mature: 27204

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSPIKNTRVYEKAIEQIKDMIVEGTFKKGDKLPSEREMAESLQISRTSIREALRELEIM
CCCCCCCHHHHHHHHHHHHHHHHHCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
GLIESRQGDGNFVKSSFENNLLKPLSTIFLLKESNPDEILELRQIIERGSVVLAAERITD
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHCCCEEEEHHHCCH
EELEEMELLFQDSSKLNFKNQLVDIDIAFHYKIAQASKNFLILSILNAISFLIEAFIKDI
HHHHHHHHHHHCCCCCCHHHHEEEEEEHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
RKNVITKKENTDMLIKQHRDILSALKEHDPMAAEEAMLSHLQYVNSQMKKEIEITT
HHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MFSPIKNTRVYEKAIEQIKDMIVEGTFKKGDKLPSEREMAESLQISRTSIREALRELEIM
CCCCCCCHHHHHHHHHHHHHHHHHCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
GLIESRQGDGNFVKSSFENNLLKPLSTIFLLKESNPDEILELRQIIERGSVVLAAERITD
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHCCCEEEEHHHCCH
EELEEMELLFQDSSKLNFKNQLVDIDIAFHYKIAQASKNFLILSILNAISFLIEAFIKDI
HHHHHHHHHHHCCCCCCHHHHEEEEEEHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
RKNVITKKENTDMLIKQHRDILSALKEHDPMAAEEAMLSHLQYVNSQMKKEIEITT
HHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]