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The map label for this gene is exoA [H]

Identifier: 148379451

GI number: 148379451

Start: 1611426

End: 1612187

Strand: Direct

Name: exoA [H]

Synonym: CBO1480

Alternate gene names: 148379451

Gene position: 1611426-1612187 (Clockwise)

Preceding gene: 148379450

Following gene: 148379452

Centisome position: 41.46

GC content: 27.95

Gene sequence:

>762_bases
ATGAGAATTTATTCTTGGAATGTTAATGGTTTAAGAGCAGTAGCAAAAAAGAACTTTATTGAGTGGATAGGTGAAGAAAG
CCCTGATATATTATGTATACAAGAAACGAAGTTACAGGAAAATCAATTGGAAGATAATATAAAAAATATAGATGGCTATT
ATTCTTATTTTAGCTTTGCAGTAAAAAAGGGATATAGTGGTGTGGCTACTTATACTAAAGTAAAACCTATTTCAGTAAAG
CATAGTATAGGTATAGAAAAATTTGATTCAGAAGGAAGAATACTTATAACAGGATTTAAGGATTTTACACTTTTAAACAT
ATATTTTCCTAATGGCCAAAGAGATGAGGAAAGACTTCAATATAAATTAGATTTTTATGAAGCATTATTTAATTATTGTG
ATGAATTAGTTAAGGAAGGTAAAAAGCTAGTTATATGTGGAGATTATAATACAGCTCATAATGAAATAGATCTTAAGAAT
CCTAAAGCTAATGAGAAAACCTCAGGATTTTTAAGAATAGAAAGAGACTGGTTAGATAAAATAATAGAAAGAGGATATAC
GGATACCTTTAGAAATATGAATCCAGATAAGATTAAATATTCTTGGTGGAGTTATAGATTTAAAGCTAGAGAAAGAAATG
CAGGTTGGAGAATCGATTATCATTTTGTTTCAAACAATTTATTAGATAGGGTAGAAAATACAGAAATATTAAATGAGGTT
TATGGTTCAGATCATTGTCCTGTAATGTTAGAGTTAAAGTAA

Upstream 100 bases:

>100_bases
ACTTTTATTATTTTACGTGTGTTTTTGAAAATTTTTTGATATAATTTATAATAGTTATTAAATAACATTATTTAGATGAA
TTTAAGAAAAGGTGAGTATT

Downstream 100 bases:

>100_bases
ATAATTACTAATATAAAATATATTAGATTATAGGTTAAATAATTTTTCTTGTTAAAATATTTTTAATACTTAGGATAATA
ATCTATTAAGAAAGTGCATT

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MRIYSWNVNGLRAVAKKNFIEWIGEESPDILCIQETKLQENQLEDNIKNIDGYYSYFSFAVKKGYSGVATYTKVKPISVK
HSIGIEKFDSEGRILITGFKDFTLLNIYFPNGQRDEERLQYKLDFYEALFNYCDELVKEGKKLVICGDYNTAHNEIDLKN
PKANEKTSGFLRIERDWLDKIIERGYTDTFRNMNPDKIKYSWWSYRFKARERNAGWRIDYHFVSNNLLDRVENTEILNEV
YGSDHCPVMLELK

Sequences:

>Translated_253_residues
MRIYSWNVNGLRAVAKKNFIEWIGEESPDILCIQETKLQENQLEDNIKNIDGYYSYFSFAVKKGYSGVATYTKVKPISVK
HSIGIEKFDSEGRILITGFKDFTLLNIYFPNGQRDEERLQYKLDFYEALFNYCDELVKEGKKLVICGDYNTAHNEIDLKN
PKANEKTSGFLRIERDWLDKIIERGYTDTFRNMNPDKIKYSWWSYRFKARERNAGWRIDYHFVSNNLLDRVENTEILNEV
YGSDHCPVMLELK
>Mature_253_residues
MRIYSWNVNGLRAVAKKNFIEWIGEESPDILCIQETKLQENQLEDNIKNIDGYYSYFSFAVKKGYSGVATYTKVKPISVK
HSIGIEKFDSEGRILITGFKDFTLLNIYFPNGQRDEERLQYKLDFYEALFNYCDELVKEGKKLVICGDYNTAHNEIDLKN
PKANEKTSGFLRIERDWLDKIIERGYTDTFRNMNPDKIKYSWWSYRFKARERNAGWRIDYHFVSNNLLDRVENTEILNEV
YGSDHCPVMLELK

Specific function: Major Apurinic-Apyrimidinic Endonuclease Of E.Coli. It Removes The Damaged DNA At Cytosines And Guanines By Cleaving On The 3' Side Of The Ap Site By A Beta-Elimination Reaction. It Exhibits 3'-5'-Exonuclease, 3'-Phosphomonoesterase, 3'-Repair Diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=256, Percent_Identity=41.40625, Blast_Score=213, Evalue=2e-55,
Organism=Homo sapiens, GI18375503, Length=256, Percent_Identity=41.40625, Blast_Score=213, Evalue=2e-55,
Organism=Homo sapiens, GI18375501, Length=256, Percent_Identity=41.40625, Blast_Score=213, Evalue=2e-55,
Organism=Homo sapiens, GI18375507, Length=314, Percent_Identity=30.5732484076433, Blast_Score=131, Evalue=5e-31,
Organism=Escherichia coli, GI1788046, Length=266, Percent_Identity=31.5789473684211, Blast_Score=123, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI71989536, Length=256, Percent_Identity=39.453125, Blast_Score=193, Evalue=9e-50,
Organism=Caenorhabditis elegans, GI71989539, Length=114, Percent_Identity=35.0877192982456, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI221330655, Length=255, Percent_Identity=41.9607843137255, Blast_Score=214, Evalue=5e-56,
Organism=Drosophila melanogaster, GI17136678, Length=255, Percent_Identity=41.9607843137255, Blast_Score=213, Evalue=8e-56,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 29919; Mature: 29919

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS00726 AP_NUCLEASE_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIYSWNVNGLRAVAKKNFIEWIGEESPDILCIQETKLQENQLEDNIKNIDGYYSYFSFA
CEEEEECCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHCCHHHHHHHHH
VKKGYSGVATYTKVKPISVKHSIGIEKFDSEGRILITGFKDFTLLNIYFPNGQRDEERLQ
HHCCCCCCEEEEEECCEEEHHHCCCEEECCCCCEEEEECCCEEEEEEECCCCCCCHHHHH
YKLDFYEALFNYCDELVKEGKKLVICGDYNTAHNEIDLKNPKANEKTSGFLRIERDWLDK
HHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEECCCCCCCCCCCCEEEEEHHHHHH
IIERGYTDTFRNMNPDKIKYSWWSYRFKARERNAGWRIDYHFVSNNLLDRVENTEILNEV
HHHCCCCHHHCCCCCCCEEEEEEEEEEEEECCCCCEEEEEEECCCHHHHHHHHHHHHHHH
YGSDHCPVMLELK
HCCCCCCEEEEEC
>Mature Secondary Structure
MRIYSWNVNGLRAVAKKNFIEWIGEESPDILCIQETKLQENQLEDNIKNIDGYYSYFSFA
CEEEEECCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHCCHHHHHHHHH
VKKGYSGVATYTKVKPISVKHSIGIEKFDSEGRILITGFKDFTLLNIYFPNGQRDEERLQ
HHCCCCCCEEEEEECCEEEHHHCCCEEECCCCCEEEEECCCEEEEEEECCCCCCCHHHHH
YKLDFYEALFNYCDELVKEGKKLVICGDYNTAHNEIDLKNPKANEKTSGFLRIERDWLDK
HHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEECCCCCCCCCCCCEEEEEHHHHHH
IIERGYTDTFRNMNPDKIKYSWWSYRFKARERNAGWRIDYHFVSNNLLDRVENTEILNEV
HHHCCCCHHHCCCCCCCEEEEEEEEEEEEECCCCCEEEEEEECCCHHHHHHHHHHHHHHH
YGSDHCPVMLELK
HCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]