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The map label for this gene is pyrP [H]

Identifier: 148379450

GI number: 148379450

Start: 1609493

End: 1610836

Strand: Direct

Name: pyrP [H]

Synonym: CBO1479

Alternate gene names: 148379450

Gene position: 1609493-1610836 (Clockwise)

Preceding gene: 148379449

Following gene: 148379451

Centisome position: 41.41

GC content: 34.3

Gene sequence:

>1344_bases
ATGAAAAAATACGTAGACGTAAATGAAAAATTACCTATCTTAAAAACTATACCTTTAAGTTTTCAACATTTATTTGCAAT
GGTGGGAGCCACTATATTAGTGCCAATGCTTACAGGTATGAGTCCTTCTATTGCATTGTTTGGAAGTGGTGTAGGAACAC
TTCTTTATGTACTTTGTACAAAAGCAAAACTACCAGCTTATATAGGATCATCTTTTGCATTTATAGGTCCTATGACAGTA
GCATCATCGGCTTATGGACCAAATGCCATGTTATCTGGAATAATAGCTGCAGGTTTAATTTATATATTAGTAGCAGCTAT
AATAAGTTTTACAGGAACAGATTGGTTAAATAAAGTATTACCACCAATTGTAGTTGGTTCCGTTGTAATAGTAATAGGGT
TAGGATTAGCGGGAGTAGCAATAAATTGGGCAGGTCTTAATTCTAATTTTATGGTAGATTCCATGCAAAGCGTACCAAGA
TGGGCATGGATTACGGTTTCAATAATAACCTTAGGAATAGGAATACTTGGAACTATGTATTTCAAAGGATTCTTAGGAGT
GATACCAATCCTTATAGCAATGATATGTGGATATATTTCAGCATTAGCCCTAGGGGTTATATCTCAGGAAACTTTAACTC
AAATAGCAAGTGCGCCTTTGTTTCAACTTCCACCATTTATGAAACCAGAGTTTAACATAAACGCCGTGGTATTAATGGCA
CCAGTAGCCTTTGTAACCTTAGCAGAACATATTGGACATGTTTATGTAACAAATAATGTAGTAGGAAAAGATTTTACAAA
AGACCCTGGCCTTCATAGATCTATACTAGGAGATGGAGTAGCTACTCTACTAGCAGGATTCATAGGTGGACCTCCTAACA
CAACTTATGGAGAAAATATTGGAGTTATGGCTATAACTAAAGTATACAGCGTATGGGTAATTATAGGAGCTGCAATAATA
GCTATTATACTTTCCTTTATAGGACCTGTAGCAACATTAATAGCTACTATACCAATGCCAGTAATGGGCGGAGTGAGTAT
ACTTTTATTTGGAATAATAGCTTCCTCAGGTTTTAGAGTATTTGTAGAGGATAAAATAGATTTTAGCAAAAAGAGAAACT
TAATAATATCCTCTGTAATAATAGTATTAGGAATAGGTGGAGCTGCAGTAAAATTTAAGTTAGCAGGATCAGAGGTAGAA
ATAGCAGGAGTTGCATTAGCAACACTAGTAGGAATAATATTAAATCTAATACTTCCAAAAGAAAGTAGAACGGAAGAAGA
GGATAATGAAGAAGAAAATCTTAAAGAAGAATTTGTTAAACCTATAGAAAAAAATGTGGGTTAA

Upstream 100 bases:

>100_bases
TCAGTACATTTAAAAGAACTTGATGGACAAGATTCCATTAAAATATTTGAAAAATAGCATCTTTTAAAATAGTCCAGAGA
GACTAAAAGGGGGAAATAAT

Downstream 100 bases:

>100_bases
AAGATTTAATGTATAATACCTTTTAAAGGTTAGGAATTAAAACAAAAAGTATTTTTAGTCAATAAATAGCTATAAAAAAT
AAAGAATACTAATATTTGTG

Product: uracil permease

Products: Proton [Cytoplasm]; uracil [Cytoplasm] [C]

Alternate protein names: Uracil transporter [H]

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MKKYVDVNEKLPILKTIPLSFQHLFAMVGATILVPMLTGMSPSIALFGSGVGTLLYVLCTKAKLPAYIGSSFAFIGPMTV
ASSAYGPNAMLSGIIAAGLIYILVAAIISFTGTDWLNKVLPPIVVGSVVIVIGLGLAGVAINWAGLNSNFMVDSMQSVPR
WAWITVSIITLGIGILGTMYFKGFLGVIPILIAMICGYISALALGVISQETLTQIASAPLFQLPPFMKPEFNINAVVLMA
PVAFVTLAEHIGHVYVTNNVVGKDFTKDPGLHRSILGDGVATLLAGFIGGPPNTTYGENIGVMAITKVYSVWVIIGAAII
AIILSFIGPVATLIATIPMPVMGGVSILLFGIIASSGFRVFVEDKIDFSKKRNLIISSVIIVLGIGGAAVKFKLAGSEVE
IAGVALATLVGIILNLILPKESRTEEEDNEEENLKEEFVKPIEKNVG

Sequences:

>Translated_447_residues
MKKYVDVNEKLPILKTIPLSFQHLFAMVGATILVPMLTGMSPSIALFGSGVGTLLYVLCTKAKLPAYIGSSFAFIGPMTV
ASSAYGPNAMLSGIIAAGLIYILVAAIISFTGTDWLNKVLPPIVVGSVVIVIGLGLAGVAINWAGLNSNFMVDSMQSVPR
WAWITVSIITLGIGILGTMYFKGFLGVIPILIAMICGYISALALGVISQETLTQIASAPLFQLPPFMKPEFNINAVVLMA
PVAFVTLAEHIGHVYVTNNVVGKDFTKDPGLHRSILGDGVATLLAGFIGGPPNTTYGENIGVMAITKVYSVWVIIGAAII
AIILSFIGPVATLIATIPMPVMGGVSILLFGIIASSGFRVFVEDKIDFSKKRNLIISSVIIVLGIGGAAVKFKLAGSEVE
IAGVALATLVGIILNLILPKESRTEEEDNEEENLKEEFVKPIEKNVG
>Mature_447_residues
MKKYVDVNEKLPILKTIPLSFQHLFAMVGATILVPMLTGMSPSIALFGSGVGTLLYVLCTKAKLPAYIGSSFAFIGPMTV
ASSAYGPNAMLSGIIAAGLIYILVAAIISFTGTDWLNKVLPPIVVGSVVIVIGLGLAGVAINWAGLNSNFMVDSMQSVPR
WAWITVSIITLGIGILGTMYFKGFLGVIPILIAMICGYISALALGVISQETLTQIASAPLFQLPPFMKPEFNINAVVLMA
PVAFVTLAEHIGHVYVTNNVVGKDFTKDPGLHRSILGDGVATLLAGFIGGPPNTTYGENIGVMAITKVYSVWVIIGAAII
AIILSFIGPVATLIATIPMPVMGGVSILLFGIIASSGFRVFVEDKIDFSKKRNLIISSVIIVLGIGGAAVKFKLAGSEVE
IAGVALATLVGIILNLILPKESRTEEEDNEEENLKEEFVKPIEKNVG

Specific function: Transport of uracil in the cell [H]

COG id: COG2233

COG function: function code F; Xanthine/uracil permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the xanthine/uracil permease family. Nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) subfamily [H]

Homologues:

Organism=Homo sapiens, GI44680145, Length=166, Percent_Identity=30.7228915662651, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI44680148, Length=172, Percent_Identity=31.3953488372093, Blast_Score=66, Evalue=6e-11,
Organism=Homo sapiens, GI40316845, Length=172, Percent_Identity=31.3953488372093, Blast_Score=66, Evalue=6e-11,
Organism=Escherichia coli, GI1788843, Length=440, Percent_Identity=47.5, Blast_Score=382, Evalue=1e-107,
Organism=Escherichia coli, GI87081818, Length=427, Percent_Identity=37.9391100702576, Blast_Score=263, Evalue=2e-71,
Organism=Escherichia coli, GI87082181, Length=469, Percent_Identity=26.6524520255864, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1790087, Length=447, Percent_Identity=25.7270693512304, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI87082178, Length=402, Percent_Identity=25.3731343283582, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17558856, Length=457, Percent_Identity=25.601750547046, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17542262, Length=179, Percent_Identity=27.9329608938547, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI21356175, Length=428, Percent_Identity=24.0654205607477, Blast_Score=69, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006042
- InterPro:   IPR006043 [H]

Pfam domain/function: PF00860 Xan_ur_permease [H]

EC number: NA

Molecular weight: Translated: 47105; Mature: 47105

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: PS01116 XANTH_URACIL_PERMASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKYVDVNEKLPILKTIPLSFQHLFAMVGATILVPMLTGMSPSIALFGSGVGTLLYVLCT
CCCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHH
KAKLPAYIGSSFAFIGPMTVASSAYGPNAMLSGIIAAGLIYILVAAIISFTGTDWLNKVL
CCCCCHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
PPIVVGSVVIVIGLGLAGVAINWAGLNSNFMVDSMQSVPRWAWITVSIITLGIGILGTMY
HHHHHHHHHHHHHHHHHHHEEEEECCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
FKGFLGVIPILIAMICGYISALALGVISQETLTQIASAPLFQLPPFMKPEFNINAVVLMA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEHHHH
PVAFVTLAEHIGHVYVTNNVVGKDFTKDPGLHRSILGDGVATLLAGFIGGPPNTTYGENI
HHHHHHHHHHHCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
GVMAITKVYSVWVIIGAAIIAIILSFIGPVATLIATIPMPVMGGVSILLFGIIASSGFRV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEE
FVEDKIDFSKKRNLIISSVIIVLGIGGAAVKFKLAGSEVEIAGVALATLVGIILNLILPK
EEECCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEHHHHHHHHHHHHHHHHHCCC
ESRTEEEDNEEENLKEEFVKPIEKNVG
CCCCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure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CCCCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; uracil [Periplasm] [C]

Specific reaction: Proton [Periplasm] + uracil [Periplasm] = Proton [Cytoplasm] + uracil [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]