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The map label for this gene is nadB [H]

Identifier: 148379420

GI number: 148379420

Start: 1579645

End: 1580946

Strand: Reverse

Name: nadB [H]

Synonym: CBO1447A

Alternate gene names: 148379420

Gene position: 1580946-1579645 (Counterclockwise)

Preceding gene: 148379421

Following gene: 148379419

Centisome position: 40.67

GC content: 27.96

Gene sequence:

>1302_bases
ATGAATCTTTATGCTGATGTCTTGATTGCAGGAAGCGGTGTAGCTGGATTATATTCAGCTTTAAATTTAAGAAAAGACTT
AAAAATAGTTTTAATCTCAAAAGGTAAACTTAACGAATGTAATACTACCCTAGCCCAGGGTGGAATCTCTGTAGCAAGGA
ATAAGGATGATATAACCACTTTTGTAGAAGATACTTTAAAAGCAGGATCCTATGAAAATAACTTGTGTGCAGTAAAAATA
TTAGCAAATGAATCTATAGAAAATATTAATAAATTGCAGGATATGGGCCTTAACTTGGATAAATGTGAAAAGGAATTAAG
CTATACAAAAGAAGGAGCTCATAGTATAAACAGAATTGTTCATTTTAAGGATTATACTGGTAAAAAATTAGAAGAAATCT
TAACTAAGAAAACACTTAAAAGAAAGAATATAACTGTAATTGAAAATTGTAGCCTTGTAGATTTAATACAAAATAATAAT
ACTTGCTTTGGAGGTATATGTTTAAAAGATAATAAGCAAATAAACATATATTCAAAAGTAACAATACTGGCAACAGGAGG
TATAGGAGGATTATTTAAAAACTCAACTAATGAACCAATTATAACTGGAGATGGTATAGCTATAGCTATAAAAAACAATA
TAAAAATTAAAAATTTGGATTATATACAATTTCATCCTACAGCATTTTTTAAGGATACTATTAATGGAAAAAAATTTTTA
ATTTCTGAGTCAGTACGAGGAGAAGGAGGAAAGCTTTTAAATTCTAAAGGTGAAAGATTTGTAAATGAATTACTCTCTAG
AGATATAGTAACCAAGTATATCCTGGAAGAGGAAAGAAAAACCGGCTCTAATAATGTATATTTAGATATCACCTTTAAAC
CAGAAACTTTTTTAAAGAAAAGATTCCCTACTATTTATGAAAATTGTTTAAAAAATGGCGTAAATATTTGTAAAGATGCT
ATTCCTGTATCTCCGGCACAACATTATTTCATGGGTGGAATACAAGTAGACTTATTTGGACAATCTTCTATGAATCATTT
ATATGCTTTTGGTGAAGTAAGTTGCACTGGAGTACACGGTAAAAATAGACTTGCTAGCAACTCCCTACTAGAAGGACTAG
TATTTTCAAAACGTGGAGCTGAAAAAATAAATTTTGAAATAAACAATATTAAATTATCATATACAAAGATTTATACATTG
AAACATAATGTAGAATATTATAGTTTATTAAATAAAAAAATAATTACAAAATCTCTACTTAAACTAAGGAGTGATTTACA
AAATGAATTGGTTAGTTGTTGA

Upstream 100 bases:

>100_bases
GTCTTTTAAATTTAAATTATAAAATAGAATTAGATAAAAATTTAAGATTAAAGGCCTATGATGCCCTTATAAATATGCAT
ACTTTAGGAGGCAGATAACT

Downstream 100 bases:

>100_bases
TGAAATACTTAAATCTGCACTTAAAGAAGATATATCCTTTGAAGACATAACTACAGAATCTATATTTAAAGAAAATAAAA
GAGCAAAAATAGATTTAATT

Product: L-aspartate oxidase

Products: NA

Alternate protein names: LASPO; Quinolinate synthase B [H]

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MNLYADVLIAGSGVAGLYSALNLRKDLKIVLISKGKLNECNTTLAQGGISVARNKDDITTFVEDTLKAGSYENNLCAVKI
LANESIENINKLQDMGLNLDKCEKELSYTKEGAHSINRIVHFKDYTGKKLEEILTKKTLKRKNITVIENCSLVDLIQNNN
TCFGGICLKDNKQINIYSKVTILATGGIGGLFKNSTNEPIITGDGIAIAIKNNIKIKNLDYIQFHPTAFFKDTINGKKFL
ISESVRGEGGKLLNSKGERFVNELLSRDIVTKYILEEERKTGSNNVYLDITFKPETFLKKRFPTIYENCLKNGVNICKDA
IPVSPAQHYFMGGIQVDLFGQSSMNHLYAFGEVSCTGVHGKNRLASNSLLEGLVFSKRGAEKINFEINNIKLSYTKIYTL
KHNVEYYSLLNKKIITKSLLKLRSDLQNELVSC

Sequences:

>Translated_433_residues
MNLYADVLIAGSGVAGLYSALNLRKDLKIVLISKGKLNECNTTLAQGGISVARNKDDITTFVEDTLKAGSYENNLCAVKI
LANESIENINKLQDMGLNLDKCEKELSYTKEGAHSINRIVHFKDYTGKKLEEILTKKTLKRKNITVIENCSLVDLIQNNN
TCFGGICLKDNKQINIYSKVTILATGGIGGLFKNSTNEPIITGDGIAIAIKNNIKIKNLDYIQFHPTAFFKDTINGKKFL
ISESVRGEGGKLLNSKGERFVNELLSRDIVTKYILEEERKTGSNNVYLDITFKPETFLKKRFPTIYENCLKNGVNICKDA
IPVSPAQHYFMGGIQVDLFGQSSMNHLYAFGEVSCTGVHGKNRLASNSLLEGLVFSKRGAEKINFEINNIKLSYTKIYTL
KHNVEYYSLLNKKIITKSLLKLRSDLQNELVSC
>Mature_433_residues
MNLYADVLIAGSGVAGLYSALNLRKDLKIVLISKGKLNECNTTLAQGGISVARNKDDITTFVEDTLKAGSYENNLCAVKI
LANESIENINKLQDMGLNLDKCEKELSYTKEGAHSINRIVHFKDYTGKKLEEILTKKTLKRKNITVIENCSLVDLIQNNN
TCFGGICLKDNKQINIYSKVTILATGGIGGLFKNSTNEPIITGDGIAIAIKNNIKIKNLDYIQFHPTAFFKDTINGKKFL
ISESVRGEGGKLLNSKGERFVNELLSRDIVTKYILEEERKTGSNNVYLDITFKPETFLKKRFPTIYENCLKNGVNICKDA
IPVSPAQHYFMGGIQVDLFGQSSMNHLYAFGEVSCTGVHGKNRLASNSLLEGLVFSKRGAEKINFEINNIKLSYTKIYTL
KHNVEYYSLLNKKIITKSLLKLRSDLQNELVSC

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate [H]

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=380, Percent_Identity=32.3684210526316, Blast_Score=181, Evalue=2e-45,
Organism=Escherichia coli, GI1788928, Length=399, Percent_Identity=39.8496240601504, Blast_Score=284, Evalue=7e-78,
Organism=Escherichia coli, GI1786942, Length=386, Percent_Identity=31.6062176165803, Blast_Score=172, Evalue=4e-44,
Organism=Escherichia coli, GI1790597, Length=384, Percent_Identity=28.90625, Blast_Score=167, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17550100, Length=388, Percent_Identity=32.2164948453608, Blast_Score=171, Evalue=5e-43,
Organism=Caenorhabditis elegans, GI17505833, Length=388, Percent_Identity=31.4432989690722, Blast_Score=169, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI71986328, Length=417, Percent_Identity=31.1750599520384, Blast_Score=121, Evalue=7e-28,
Organism=Saccharomyces cerevisiae, GI6322701, Length=395, Percent_Identity=31.6455696202532, Blast_Score=168, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6322416, Length=394, Percent_Identity=30.9644670050761, Blast_Score=168, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6320788, Length=477, Percent_Identity=27.2536687631027, Blast_Score=90, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6322511, Length=306, Percent_Identity=29.7385620915033, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI17137288, Length=449, Percent_Identity=30.0668151447661, Blast_Score=171, Evalue=7e-43,
Organism=Drosophila melanogaster, GI24655642, Length=449, Percent_Identity=30.0668151447661, Blast_Score=171, Evalue=7e-43,
Organism=Drosophila melanogaster, GI24655647, Length=449, Percent_Identity=30.0668151447661, Blast_Score=171, Evalue=7e-43,
Organism=Drosophila melanogaster, GI24663005, Length=393, Percent_Identity=29.5165394402036, Blast_Score=157, Evalue=2e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027 [H]

Pfam domain/function: PF00890 FAD_binding_2 [H]

EC number: =1.4.3.16 [H]

Molecular weight: Translated: 48296; Mature: 48296

Theoretical pI: Translated: 9.38; Mature: 9.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLYADVLIAGSGVAGLYSALNLRKDLKIVLISKGKLNECNTTLAQGGISVARNKDDITT
CCEEEEEEEECCCHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHCCCEEECCCHHHHH
FVEDTLKAGSYENNLCAVKILANESIENINKLQDMGLNLDKCEKELSYTKEGAHSINRIV
HHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHEE
HFKDYTGKKLEEILTKKTLKRKNITVIENCSLVDLIQNNNTCFGGICLKDNKQINIYSKV
EECCCCCHHHHHHHHHHHHHHCCCEEEECCCEEEEEECCCEEEEEEEECCCCEEEEEEEE
TILATGGIGGLFKNSTNEPIITGDGIAIAIKNNIKIKNLDYIQFHPTAFFKDTINGKKFL
EEEEECCCCCCCCCCCCCCEEECCCEEEEEECCEEEEECEEEEECCCEEEHHCCCCCEEE
ISESVRGEGGKLLNSKGERFVNELLSRDIVTKYILEEERKTGSNNVYLDITFKPETFLKK
EECCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCHHHHHH
RFPTIYENCLKNGVNICKDAIPVSPAQHYFMGGIQVDLFGQSSMNHLYAFGEVSCTGVHG
CCHHHHHHHHHCCHHHHHHCCCCCCCHHHEECCEEEEEECCCCCCCEEEECCEEEEECCC
KNRLASNSLLEGLVFSKRGAEKINFEINNIKLSYTKIYTLKHNVEYYSLLNKKIITKSLL
CCHHHHHHHHHHHHHCCCCCEEEEEEEEEEEEEEEEEEEEEECCHHHHHHHHHHHHHHHH
KLRSDLQNELVSC
HHHHHHHHHHHCC
>Mature Secondary Structure
MNLYADVLIAGSGVAGLYSALNLRKDLKIVLISKGKLNECNTTLAQGGISVARNKDDITT
CCEEEEEEEECCCHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHCCCEEECCCHHHHH
FVEDTLKAGSYENNLCAVKILANESIENINKLQDMGLNLDKCEKELSYTKEGAHSINRIV
HHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHEE
HFKDYTGKKLEEILTKKTLKRKNITVIENCSLVDLIQNNNTCFGGICLKDNKQINIYSKV
EECCCCCHHHHHHHHHHHHHHCCCEEEECCCEEEEEECCCEEEEEEEECCCCEEEEEEEE
TILATGGIGGLFKNSTNEPIITGDGIAIAIKNNIKIKNLDYIQFHPTAFFKDTINGKKFL
EEEEECCCCCCCCCCCCCCEEECCCEEEEEECCEEEEECEEEEECCCEEEHHCCCCCEEE
ISESVRGEGGKLLNSKGERFVNELLSRDIVTKYILEEERKTGSNNVYLDITFKPETFLKK
EECCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCHHHHHH
RFPTIYENCLKNGVNICKDAIPVSPAQHYFMGGIQVDLFGQSSMNHLYAFGEVSCTGVHG
CCHHHHHHHHHCCHHHHHHCCCCCCCHHHEECCEEEEEECCCCCCCEEEECCEEEEECCC
KNRLASNSLLEGLVFSKRGAEKINFEINNIKLSYTKIYTLKHNVEYYSLLNKKIITKSLL
CCHHHHHHHHHHHHHCCCCCEEEEEEEEEEEEEEEEEEEEEECCHHHHHHHHHHHHHHHH
KLRSDLQNELVSC
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11792842 [H]