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The map label for this gene is trxB1 [C]

Identifier: 148379239

GI number: 148379239

Start: 1370028

End: 1370969

Strand: Reverse

Name: trxB1 [C]

Synonym: CBO1254

Alternate gene names: 148379239

Gene position: 1370969-1370028 (Counterclockwise)

Preceding gene: 148379255

Following gene: 148379238

Centisome position: 35.27

GC content: 30.57

Gene sequence:

>942_bases
ATGAATAAAAAAAATAAAAACTTAGATTTAATGATTATAGGAGCAGGTCCTGCAGGATTATCTGCTGCTATATATGCTGC
TAGAGCTAAGCTTAACATGATTTTAATTGAAAATGGAATAATTGGTGGTCAAGTTAGAAGTAGTTATACCGTTGAAAATT
ATCCTGGATTTAAAAAAATTGAAGGTAACCGTCTTGCAGATTTAATGCAAGAGCAAGCTACAGATCTTGGTGCTACTATA
GATGAATTTGATATGGTAGAAAAAATTCAATTAAATAATAACGAAAAAATAGTTGAAACTTCAGAATATATATACAAACC
AACAGTCTTAATTATATCTACTGGCGCTACAGCTAGAAAGCTCCCTATCCCTAGTGAGGGTAAGTTTTCTGGGAAAGGTA
TACACTACTGTGCTATTTGCGATGGTGCTATGTACGAAGGCAAAACTGTTGGAGTAATTGGCGGTGGAAATGCCGCCTTA
GAAGAAGCTTTGTTTTTAACCAAATTCGCTTCAAAAGTATATATGATCAGAAGATATGATTATTTTAAAGGTGAAAAAGC
CACACTAGAAGAAGTTGAAAACAATTCTAAAATAGAAATATTATACAACTGGGATTTAGTAGATGTTTATGGTTCAAATT
TTATAGATAAAGCTTTAATTAAAAATGTTAAAACAAAAGAAGAAAAAGAATTAAAATTAGATGGGGTTTTTGGATTTATA
GGTTCAGAACCTAAAACAGAATTATTTAAAGAATATATATCTTTAACTCCTAAAGGATATATTAAAACTAATGAAAATAT
GGAAACCAATATAAAAGGTGTTTATGCCGCTGGAGATGTTAGAGAAAAACAATTTCGCCAAATAACTACTGCTGTAGCTG
ATGGAACTATTGCTGCATTAGCTGCTGAAAAATATATTATGGAAAAAACAAGAGAAAAATAA

Upstream 100 bases:

>100_bases
ATATTTTTTTATTAATAATTTTTATTAATTATATTGTGAAACACAAAAAAAGTAGTATAATTAATATATACCCTATAAGG
GTAATAAGGGGTGATTTTAT

Downstream 100 bases:

>100_bases
TATTTATATATTACAAAAGAAAAGATTAGAGGTGTCTATATGTTTAAAAATTTATACGTAGAAAACAATTTATATAAAAA
TTTTATAGGAGGACAATGGG

Product: putative thioredoxin reductase

Products: thioredoxin disulfide; NADPH; H+

Alternate protein names: ORF A [H]

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MNKKNKNLDLMIIGAGPAGLSAAIYAARAKLNMILIENGIIGGQVRSSYTVENYPGFKKIEGNRLADLMQEQATDLGATI
DEFDMVEKIQLNNNEKIVETSEYIYKPTVLIISTGATARKLPIPSEGKFSGKGIHYCAICDGAMYEGKTVGVIGGGNAAL
EEALFLTKFASKVYMIRRYDYFKGEKATLEEVENNSKIEILYNWDLVDVYGSNFIDKALIKNVKTKEEKELKLDGVFGFI
GSEPKTELFKEYISLTPKGYIKTNENMETNIKGVYAAGDVREKQFRQITTAVADGTIAALAAEKYIMEKTREK

Sequences:

>Translated_313_residues
MNKKNKNLDLMIIGAGPAGLSAAIYAARAKLNMILIENGIIGGQVRSSYTVENYPGFKKIEGNRLADLMQEQATDLGATI
DEFDMVEKIQLNNNEKIVETSEYIYKPTVLIISTGATARKLPIPSEGKFSGKGIHYCAICDGAMYEGKTVGVIGGGNAAL
EEALFLTKFASKVYMIRRYDYFKGEKATLEEVENNSKIEILYNWDLVDVYGSNFIDKALIKNVKTKEEKELKLDGVFGFI
GSEPKTELFKEYISLTPKGYIKTNENMETNIKGVYAAGDVREKQFRQITTAVADGTIAALAAEKYIMEKTREK
>Mature_313_residues
MNKKNKNLDLMIIGAGPAGLSAAIYAARAKLNMILIENGIIGGQVRSSYTVENYPGFKKIEGNRLADLMQEQATDLGATI
DEFDMVEKIQLNNNEKIVETSEYIYKPTVLIISTGATARKLPIPSEGKFSGKGIHYCAICDGAMYEGKTVGVIGGGNAAL
EEALFLTKFASKVYMIRRYDYFKGEKATLEEVENNSKIEILYNWDLVDVYGSNFIDKALIKNVKTKEEKELKLDGVFGFI
GSEPKTELFKEYISLTPKGYIKTNENMETNIKGVYAAGDVREKQFRQITTAVADGTIAALAAEKYIMEKTREK

Specific function: Unknown

COG id: COG0492

COG function: function code O; Thioredoxin reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI1787114, Length=312, Percent_Identity=38.7820512820513, Blast_Score=197, Evalue=5e-52,
Organism=Escherichia coli, GI87081763, Length=320, Percent_Identity=29.6875, Blast_Score=126, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6320560, Length=317, Percent_Identity=39.4321766561514, Blast_Score=207, Evalue=2e-54,
Organism=Saccharomyces cerevisiae, GI6321898, Length=317, Percent_Identity=36.5930599369085, Blast_Score=196, Evalue=3e-51,

Paralogues:

None

Copy number: 808 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR008255
- InterPro:   IPR001327
- InterPro:   IPR000103 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.8.1.9

Molecular weight: Translated: 34717; Mature: 34717

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS00573 PYRIDINE_REDOX_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKKNKNLDLMIIGAGPAGLSAAIYAARAKLNMILIENGIIGGQVRSSYTVENYPGFKKI
CCCCCCCEEEEEEECCCCCHHHHHHHHHHEEEEEEEECCEECCCCCCCEEECCCCCCEEE
EGNRLADLMQEQATDLGATIDEFDMVEKIQLNNNEKIVETSEYIYKPTVLIISTGATARK
CCCHHHHHHHHHHHHHCCCHHHHHHHHEEEECCCCCEEECCHHEECCEEEEEECCCCCEE
LPIPSEGKFSGKGIHYCAICDGAMYEGKTVGVIGGGNAALEEALFLTKFASKVYMIRRYD
CCCCCCCCCCCCCEEEEEEECCCEECCCEEEEEECCCHHHHHHHHHHHHHHHEEEEEEEC
YFKGEKATLEEVENNSKIEILYNWDLVDVYGSNFIDKALIKNVKTKEEKELKLDGVFGFI
CCCCCCHHHHHHCCCCEEEEEEECEEEEECCCCHHHHHHHHHCCCCCCHHEEEEEEEEEC
GSEPKTELFKEYISLTPKGYIKTNENMETNIKGVYAAGDVREKQFRQITTAVADGTIAAL
CCCCHHHHHHHHHHCCCCCEEEECCCCCCCCEEEEEECCHHHHHHHHHHHHHCCCHHHHH
AAEKYIMEKTREK
HHHHHHHHHHCCC
>Mature Secondary Structure
MNKKNKNLDLMIIGAGPAGLSAAIYAARAKLNMILIENGIIGGQVRSSYTVENYPGFKKI
CCCCCCCEEEEEEECCCCCHHHHHHHHHHEEEEEEEECCEECCCCCCCEEECCCCCCEEE
EGNRLADLMQEQATDLGATIDEFDMVEKIQLNNNEKIVETSEYIYKPTVLIISTGATARK
CCCHHHHHHHHHHHHHCCCHHHHHHHHEEEECCCCCEEECCHHEECCEEEEEECCCCCEE
LPIPSEGKFSGKGIHYCAICDGAMYEGKTVGVIGGGNAALEEALFLTKFASKVYMIRRYD
CCCCCCCCCCCCCEEEEEEECCCEECCCEEEEEECCCHHHHHHHHHHHHHHHEEEEEEEC
YFKGEKATLEEVENNSKIEILYNWDLVDVYGSNFIDKALIKNVKTKEEKELKLDGVFGFI
CCCCCCHHHHHHCCCCEEEEEEECEEEEECCCCHHHHHHHHHCCCCCCHHEEEEEEEEEC
GSEPKTELFKEYISLTPKGYIKTNENMETNIKGVYAAGDVREKQFRQITTAVADGTIAAL
CCCCHHHHHHHHHHCCCCCEEEECCCCCCCCEEEEEECCHHHHHHHHHHHHHCCCHHHHH
AAEKYIMEKTREK
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD [C]

Metal ions: NA

Kcat value (1/min): 1365 [C]

Specific activity: NA

Km value (mM): 0.008 {NADPH}} 0.125 {thioredoxin} 0.002 {thioredoxin}} 0.0017 {thioredoxin}} [C]

Substrates: thioredoxin; NADP+

Specific reaction: thioredoxin + NADP+ = thioredoxin disulfide + NADPH + H+

General reaction: Redox reaction [C]

Inhibitor: Ca2+; N-Ethyl maleimide; NADP+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1637309 [H]