Definition Clostridium botulinum A str. ATCC 3502, complete genome.
Accession NC_009495
Length 3,886,916

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The map label for this gene is argS

Identifier: 148379062

GI number: 148379062

Start: 1182492

End: 1184183

Strand: Direct

Name: argS

Synonym: CBO1072

Alternate gene names: 148379062

Gene position: 1182492-1184183 (Clockwise)

Preceding gene: 148379061

Following gene: 148379063

Centisome position: 30.42

GC content: 30.38

Gene sequence:

>1692_bases
ATGGATTACAAAAATCTAGTAGCTGAAAGAATAAAAGAAAATACAGAATTAGAAGTTGATTTAATAGAAAAACTTATAGA
GATTCCACCAAAGAAAGAAATGGGAGATTATGCTTTCCCATGCTTCCAATTAGCTAAAACTTTTAGAAAAGCACCAAATT
TAATAGCAGAAGAATTAAAAGAAAAAATAAATAAAGAAGGTTTTGAAAAGGTAGTAACTGTAGGACCTTATTTAAATTTC
TTTGTAGATAAAACTATACTCATAAAAGACGTATTAGAAAAAGTTTTAAGTGAAAAAGAAAAATACGGTTCTTCTAAAGT
AGGAGAAGGAAAAAATGTAGTAGTTGAATATTCATCACCAAACATTGCGAAACCATTCCATATAGGGCATTTATTTACCA
CAGCTATAGGTAATGCACTATATAAAATACTATCCTTTGAAGGTTATAATTGTATAGGAATTAACCATCTAGGAGATTGG
GGAACACAATTTGGTAAACTTATTTCTGCATATAGAAGATGGGTAGATGAAGAAGCACTAGAAAAGGATGCTATAGGAGA
ACTCTTAAGAATATATGTTAAATTTCATGGAGAAGCAGAAAAAGACCCTGAACTTGAAAAAGAAGCTAGATTAAACTTTA
AAAGATTAGAGGAAGGTTCTGAGGAAGAAACTGAATTATGGAATAGATTTAAAGATCTAAGCTTAAAAGAATTCAACAAG
GTTTATGACATGCTAGGAATAAAATTTGACTCTCTTGCAGGAGAAAGTTTCTATAGTGATAAAATGGATGCAGTAGTTCA
AGAAATAGATGATAAAGGATTATTAGTAGATAGCAATGGTGCAAAGGTAGTTATGCTTGATGAATACAATATGCCACCTT
GCATGATTAAAAAATCTGATGGAGCAACTATTTATGCTACTCGTGATTTAGCAGCAGCTATATACAGAAAGAAAACTTAT
GATTTCCACAAATGTATATATGTAGTCGGAACTCCTCAAGCACTACACTTTAAACAAGTATTTACTACTTTAAAACTTAT
GGGACACGATTGGGCAGATGATTGCAAACATGTAGGCTTTGGCCTTGTTAAATTAGCAAATAAAAAGCTATCTACAAGAA
ATGGAGATGTTGTATTCTTAGAAGACTTGTTAAATCAATCTGTAGAAGAAACATTAAAAATTATAAATGAGAAAAATCCT
AATTTGAAAAACAAAGGAGATGTGGCTAAAAAACTTGGTATAGGTGCAGTTGTATTCACATATCTAAAAAACAATAGAGA
AAGAGATATTGTATTTGATTGGAAGGAAATTCTATCATTTGATGGAGAAACAGGACCTTATGTAGAATATAGCTATGCAA
GAGGAAAGAGTATATTAAGAAAAGCAGGAGAATTAACTGGAGAAGCAGATTATTCTAAATTATCATCTAAAGAAGAGTTT
GAATTAGCAAAACTTCTTGGAGGCTTTAATGATGCTATAATGAATGCTATAGATAAATTAGAACCAGCTATGGTAACTAG
ATATGTAATTGAAGTAGCAAAAGCTTTCAACAAATTCTATAATGCTCATGGTATATTAAATGCAGAAGATAATGATGTAA
AATTAGCAAGGGTAAAACTTGTAGAAGCTACTTGTCAAGTAATAAAGAATGCTCTTAACCTATTAGGTATAGATGTAGTT
GAAGAAATGTAA

Upstream 100 bases:

>100_bases
ATATAAAAAATTAAAAATGCTGTTAAATGAATAAATTAATTTTAAATATTAAAATCCTATAATAAAACTTAAATATAAAA
ACTTGGAGGTAGATTAAACT

Downstream 100 bases:

>100_bases
AAAATAAAGTAAATAATTTTATTTATTGAAGAGGGTGTCTTAAAAATTAAATCTATTTCTAAGGCATCCTTTTTAATTTA
ATTAATATAATTGGATATAC

Product: arginyl-tRNA synthetase

Products: NA

Alternate protein names: Arginine--tRNA ligase; ArgRS

Number of amino acids: Translated: 563; Mature: 563

Protein sequence:

>563_residues
MDYKNLVAERIKENTELEVDLIEKLIEIPPKKEMGDYAFPCFQLAKTFRKAPNLIAEELKEKINKEGFEKVVTVGPYLNF
FVDKTILIKDVLEKVLSEKEKYGSSKVGEGKNVVVEYSSPNIAKPFHIGHLFTTAIGNALYKILSFEGYNCIGINHLGDW
GTQFGKLISAYRRWVDEEALEKDAIGELLRIYVKFHGEAEKDPELEKEARLNFKRLEEGSEEETELWNRFKDLSLKEFNK
VYDMLGIKFDSLAGESFYSDKMDAVVQEIDDKGLLVDSNGAKVVMLDEYNMPPCMIKKSDGATIYATRDLAAAIYRKKTY
DFHKCIYVVGTPQALHFKQVFTTLKLMGHDWADDCKHVGFGLVKLANKKLSTRNGDVVFLEDLLNQSVEETLKIINEKNP
NLKNKGDVAKKLGIGAVVFTYLKNNRERDIVFDWKEILSFDGETGPYVEYSYARGKSILRKAGELTGEADYSKLSSKEEF
ELAKLLGGFNDAIMNAIDKLEPAMVTRYVIEVAKAFNKFYNAHGILNAEDNDVKLARVKLVEATCQVIKNALNLLGIDVV
EEM

Sequences:

>Translated_563_residues
MDYKNLVAERIKENTELEVDLIEKLIEIPPKKEMGDYAFPCFQLAKTFRKAPNLIAEELKEKINKEGFEKVVTVGPYLNF
FVDKTILIKDVLEKVLSEKEKYGSSKVGEGKNVVVEYSSPNIAKPFHIGHLFTTAIGNALYKILSFEGYNCIGINHLGDW
GTQFGKLISAYRRWVDEEALEKDAIGELLRIYVKFHGEAEKDPELEKEARLNFKRLEEGSEEETELWNRFKDLSLKEFNK
VYDMLGIKFDSLAGESFYSDKMDAVVQEIDDKGLLVDSNGAKVVMLDEYNMPPCMIKKSDGATIYATRDLAAAIYRKKTY
DFHKCIYVVGTPQALHFKQVFTTLKLMGHDWADDCKHVGFGLVKLANKKLSTRNGDVVFLEDLLNQSVEETLKIINEKNP
NLKNKGDVAKKLGIGAVVFTYLKNNRERDIVFDWKEILSFDGETGPYVEYSYARGKSILRKAGELTGEADYSKLSSKEEF
ELAKLLGGFNDAIMNAIDKLEPAMVTRYVIEVAKAFNKFYNAHGILNAEDNDVKLARVKLVEATCQVIKNALNLLGIDVV
EEM
>Mature_563_residues
MDYKNLVAERIKENTELEVDLIEKLIEIPPKKEMGDYAFPCFQLAKTFRKAPNLIAEELKEKINKEGFEKVVTVGPYLNF
FVDKTILIKDVLEKVLSEKEKYGSSKVGEGKNVVVEYSSPNIAKPFHIGHLFTTAIGNALYKILSFEGYNCIGINHLGDW
GTQFGKLISAYRRWVDEEALEKDAIGELLRIYVKFHGEAEKDPELEKEARLNFKRLEEGSEEETELWNRFKDLSLKEFNK
VYDMLGIKFDSLAGESFYSDKMDAVVQEIDDKGLLVDSNGAKVVMLDEYNMPPCMIKKSDGATIYATRDLAAAIYRKKTY
DFHKCIYVVGTPQALHFKQVFTTLKLMGHDWADDCKHVGFGLVKLANKKLSTRNGDVVFLEDLLNQSVEETLKIINEKNP
NLKNKGDVAKKLGIGAVVFTYLKNNRERDIVFDWKEILSFDGETGPYVEYSYARGKSILRKAGELTGEADYSKLSSKEEF
ELAKLLGGFNDAIMNAIDKLEPAMVTRYVIEVAKAFNKFYNAHGILNAEDNDVKLARVKLVEATCQVIKNALNLLGIDVV
EEM

Specific function: Unknown

COG id: COG0018

COG function: function code J; Arginyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI197100773, Length=555, Percent_Identity=34.4144144144144, Blast_Score=311, Evalue=7e-85,
Organism=Homo sapiens, GI15149476, Length=612, Percent_Identity=30.0653594771242, Blast_Score=236, Evalue=5e-62,
Organism=Escherichia coli, GI1788184, Length=562, Percent_Identity=32.5622775800712, Blast_Score=288, Evalue=9e-79,
Organism=Caenorhabditis elegans, GI71985061, Length=571, Percent_Identity=30.4728546409807, Blast_Score=207, Evalue=1e-53,
Organism=Caenorhabditis elegans, GI71985068, Length=539, Percent_Identity=30.7977736549165, Blast_Score=197, Evalue=2e-50,
Organism=Caenorhabditis elegans, GI17532153, Length=501, Percent_Identity=27.1457085828343, Blast_Score=167, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6320548, Length=578, Percent_Identity=36.3321799307958, Blast_Score=348, Evalue=1e-96,
Organism=Saccharomyces cerevisiae, GI6321883, Length=575, Percent_Identity=34.0869565217391, Blast_Score=343, Evalue=4e-95,
Organism=Drosophila melanogaster, GI28571570, Length=590, Percent_Identity=28.6440677966102, Blast_Score=230, Evalue=2e-60,
Organism=Drosophila melanogaster, GI18859963, Length=541, Percent_Identity=31.2384473197782, Blast_Score=218, Evalue=9e-57,

Paralogues:

None

Copy number: 789 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYR_CLOB1 (A7FSW7)

Other databases:

- EMBL:   CP000726
- RefSeq:   YP_001383444.1
- ProteinModelPortal:   A7FSW7
- SMR:   A7FSW7
- STRING:   A7FSW7
- GeneID:   5394617
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_1112
- eggNOG:   COG0018
- HOGENOM:   HBG695395
- OMA:   AYKKWGN
- ProtClustDB:   PRK01611
- BioCyc:   CBOT441770:CLB_1112-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00123
- InterPro:   IPR001412
- InterPro:   IPR001278
- InterPro:   IPR015945
- InterPro:   IPR005148
- InterPro:   IPR008909
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.30.1360.70
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11956
- PRINTS:   PR01038
- SMART:   SM00836
- TIGRFAMs:   TIGR00456

Pfam domain/function: PF03485 Arg_tRNA_synt_N; PF05746 DALR_1; PF00750 tRNA-synt_1d; SSF55190 Arg-tRNA-synth_Ic_N; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.19

Molecular weight: Translated: 63869; Mature: 63869

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure: 

>Translated Secondary Structure
MDYKNLVAERIKENTELEVDLIEKLIEIPPKKEMGDYAFPCFQLAKTFRKAPNLIAEELK
CCHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHH
EKINKEGFEKVVTVGPYLNFFVDKTILIKDVLEKVLSEKEKYGSSKVGEGKNVVVEYSSP
HHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCC
NIAKPFHIGHLFTTAIGNALYKILSFEGYNCIGINHLGDWGTQFGKLISAYRRWVDEEAL
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
EKDAIGELLRIYVKFHGEAEKDPELEKEARLNFKRLEEGSEEETELWNRFKDLSLKEFNK
HHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCHHHHHCCCHHHHHHHHHHHCCCHHHHHH
VYDMLGIKFDSLAGESFYSDKMDAVVQEIDDKGLLVDSNGAKVVMLDEYNMPPCMIKKSD
HHHHHCCCHHHHCCCCHHHHHHHHHHHHHCCCCEEEECCCCEEEEEECCCCCCCEEECCC
GATIYATRDLAAAIYRKKTYDFHKCIYVVGTPQALHFKQVFTTLKLMGHDWADDCKHVGF
CCEEEEHHHHHHHHHHHHCCCHHHEEEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHH
GLVKLANKKLSTRNGDVVFLEDLLNQSVEETLKIINEKNPNLKNKGDVAKKLGIGAVVFT
HHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
YLKNNRERDIVFDWKEILSFDGETGPYVEYSYARGKSILRKAGELTGEADYSKLSSKEEF
HHHCCCCCCEEEEHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHCCCCCHHHHCCCHHHH
ELAKLLGGFNDAIMNAIDKLEPAMVTRYVIEVAKAFNKFYNAHGILNAEDNDVKLARVKL
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEHHHHHH
VEATCQVIKNALNLLGIDVVEEM
HHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDYKNLVAERIKENTELEVDLIEKLIEIPPKKEMGDYAFPCFQLAKTFRKAPNLIAEELK
CCHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHH
EKINKEGFEKVVTVGPYLNFFVDKTILIKDVLEKVLSEKEKYGSSKVGEGKNVVVEYSSP
HHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCC
NIAKPFHIGHLFTTAIGNALYKILSFEGYNCIGINHLGDWGTQFGKLISAYRRWVDEEAL
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
EKDAIGELLRIYVKFHGEAEKDPELEKEARLNFKRLEEGSEEETELWNRFKDLSLKEFNK
HHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCHHHHHCCCHHHHHHHHHHHCCCHHHHHH
VYDMLGIKFDSLAGESFYSDKMDAVVQEIDDKGLLVDSNGAKVVMLDEYNMPPCMIKKSD
HHHHHCCCHHHHCCCCHHHHHHHHHHHHHCCCCEEEECCCCEEEEEECCCCCCCEEECCC
GATIYATRDLAAAIYRKKTYDFHKCIYVVGTPQALHFKQVFTTLKLMGHDWADDCKHVGF
CCEEEEHHHHHHHHHHHHCCCHHHEEEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHH
GLVKLANKKLSTRNGDVVFLEDLLNQSVEETLKIINEKNPNLKNKGDVAKKLGIGAVVFT
HHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
YLKNNRERDIVFDWKEILSFDGETGPYVEYSYARGKSILRKAGELTGEADYSKLSSKEEF
HHHCCCCCCEEEEHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHCCCCCHHHHCCCHHHH
ELAKLLGGFNDAIMNAIDKLEPAMVTRYVIEVAKAFNKFYNAHGILNAEDNDVKLARVKL
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEHHHHHH
VEATCQVIKNALNLLGIDVVEEM
HHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA