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The map label for this gene is aspC [H]

Identifier: 148379009

GI number: 148379009

Start: 1121388

End: 1122677

Strand: Reverse

Name: aspC [H]

Synonym: CBO1019

Alternate gene names: 148379009

Gene position: 1122677-1121388 (Counterclockwise)

Preceding gene: 148379013

Following gene: 148379005

Centisome position: 28.88

GC content: 29.07

Gene sequence:

>1290_bases
ATGAAAAGAACATTAATAAGTGAATTGAAAAATTTTATGAATGAAAAAATAAATCTTCAAGGATGGATTCATAAAATTAG
AAAATTGAGGAATATAACATTTTTAATAATAAGAGATAGATCAGGATTGATTCAATGTATTGTAGATAATGATAAATTGG
ATTTATCGCAGGTAAAGATAGAATCTATAGTGTCATTGACAGGAGTTGTTAAAGAAAGTACTAATGCTTTAAATCCTTTT
GAAATACAAGTAGAAAATTTAGAAATTATAAATGCTGCTTTAGATAAACTTCCAATAGAAATAAATAAAGAAAATCTAGA
GATTAATTTAGATACTATGCTTAATAATAGGATATTAAGCTTAAGGCACCCTAAGGTAAATTCAATATTTAAAGTTCAAA
ACTCAATAGTAAATGGATTTAGAGAATTTTTAAATAAAGAAGGATTTACTGAAATTTATACTCCAAAGATTGTATGGGAA
GGAGCTGAAGGTGGTACAGAAGTATTTAAGGTAAAATACTTTGAAAATACTGCTTATTTAGCCCAAAGCCCTCAATTCTA
CAAACAGATGATGGTAGGTGCTGGATTTGAAAGAGTATTTGAAATAGGTCATGCCTATAGGGCAGAAGAGCATAATACTA
ATAGACATCTAAATGAATATATTAGTATGGACTTGGAAATTGGATTTATAGAGGATGAAAAAGATATTATGGAAGTTGAG
GAAAGGTTACTAAAGTTCATATTAGAAAAACTTAATAAAGAATGTAAGGAATACTTTGACCTACTGGATTCAGAATTACC
AAAAATAGAATCCGAAATACCTAAGATTACATTTAAGGAAGCATTAGATATATTAATTAAGGAATATAATAAAAATAATT
TAACTTGTGACTTAGATCCAGAAGGAGAAAAACTCATATGTAAGTATGCAAAGGAAAAGCTAGATAGTGATTTCATATTT
TTAACTAACTATCCTAGAGAAAAGAGACCAATGTATACCATGCCCCTAGGTAAAGATGGAACTCATAGTTTCGATCTGTT
ATTTAGAGGTATAGAGATTACTACAGGTGGGCAAAGAATTCACGACTATAATATGCTAGTAGATAATATGGAATTTAAAG
GATTTAATCCTAAGGACTATGAGAGTTATTTATCTGCTTTTAAGTATGGAATGCCTAAGCATGGAGGCCTTGCCATTGGA
TTAGAACGACTAACATCAAGACTTCTAGGATTAGAAAATGTTAGAGAAGCTGCATTAATTACAAGAGATAGAACTCGTTT
ACTTCCTTAA

Upstream 100 bases:

>100_bases
CTAATTTTGTATAAACATTGTTAAAAAGGGTGGTACCGCAAATTATTTTGCCCCTTGGGTTTTTACTCAAGGGGCTTATT
TTATTTAGGAGGTAATAAAT

Downstream 100 bases:

>100_bases
AGTTACTCTATTATCACAATTGTCTTCCTAGCTCTTCATAAATTTCAATATCTTATTTATTCATAAGTATCTTAGGGAGG
AACATTAATAATTTACTCCC

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS [H]

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MKRTLISELKNFMNEKINLQGWIHKIRKLRNITFLIIRDRSGLIQCIVDNDKLDLSQVKIESIVSLTGVVKESTNALNPF
EIQVENLEIINAALDKLPIEINKENLEINLDTMLNNRILSLRHPKVNSIFKVQNSIVNGFREFLNKEGFTEIYTPKIVWE
GAEGGTEVFKVKYFENTAYLAQSPQFYKQMMVGAGFERVFEIGHAYRAEEHNTNRHLNEYISMDLEIGFIEDEKDIMEVE
ERLLKFILEKLNKECKEYFDLLDSELPKIESEIPKITFKEALDILIKEYNKNNLTCDLDPEGEKLICKYAKEKLDSDFIF
LTNYPREKRPMYTMPLGKDGTHSFDLLFRGIEITTGGQRIHDYNMLVDNMEFKGFNPKDYESYLSAFKYGMPKHGGLAIG
LERLTSRLLGLENVREAALITRDRTRLLP

Sequences:

>Translated_429_residues
MKRTLISELKNFMNEKINLQGWIHKIRKLRNITFLIIRDRSGLIQCIVDNDKLDLSQVKIESIVSLTGVVKESTNALNPF
EIQVENLEIINAALDKLPIEINKENLEINLDTMLNNRILSLRHPKVNSIFKVQNSIVNGFREFLNKEGFTEIYTPKIVWE
GAEGGTEVFKVKYFENTAYLAQSPQFYKQMMVGAGFERVFEIGHAYRAEEHNTNRHLNEYISMDLEIGFIEDEKDIMEVE
ERLLKFILEKLNKECKEYFDLLDSELPKIESEIPKITFKEALDILIKEYNKNNLTCDLDPEGEKLICKYAKEKLDSDFIF
LTNYPREKRPMYTMPLGKDGTHSFDLLFRGIEITTGGQRIHDYNMLVDNMEFKGFNPKDYESYLSAFKYGMPKHGGLAIG
LERLTSRLLGLENVREAALITRDRTRLLP
>Mature_429_residues
MKRTLISELKNFMNEKINLQGWIHKIRKLRNITFLIIRDRSGLIQCIVDNDKLDLSQVKIESIVSLTGVVKESTNALNPF
EIQVENLEIINAALDKLPIEINKENLEINLDTMLNNRILSLRHPKVNSIFKVQNSIVNGFREFLNKEGFTEIYTPKIVWE
GAEGGTEVFKVKYFENTAYLAQSPQFYKQMMVGAGFERVFEIGHAYRAEEHNTNRHLNEYISMDLEIGFIEDEKDIMEVE
ERLLKFILEKLNKECKEYFDLLDSELPKIESEIPKITFKEALDILIKEYNKNNLTCDLDPEGEKLICKYAKEKLDSDFIF
LTNYPREKRPMYTMPLGKDGTHSFDLLFRGIEITTGGQRIHDYNMLVDNMEFKGFNPKDYESYLSAFKYGMPKHGGLAIG
LERLTSRLLGLENVREAALITRDRTRLLP

Specific function: Unknown

COG id: COG0017

COG function: function code J; Aspartyl/asparaginyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI45439306, Length=445, Percent_Identity=34.6067415730337, Blast_Score=266, Evalue=4e-71,
Organism=Homo sapiens, GI4758762, Length=442, Percent_Identity=26.9230769230769, Blast_Score=162, Evalue=4e-40,
Organism=Homo sapiens, GI226491502, Length=445, Percent_Identity=26.7415730337079, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI40789249, Length=252, Percent_Identity=31.3492063492063, Blast_Score=104, Evalue=2e-22,
Organism=Escherichia coli, GI1787161, Length=458, Percent_Identity=25.3275109170306, Blast_Score=131, Evalue=8e-32,
Organism=Escherichia coli, GI1788173, Length=252, Percent_Identity=29.3650793650794, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1789256, Length=451, Percent_Identity=24.8337028824834, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1790571, Length=451, Percent_Identity=23.7250554323725, Blast_Score=90, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17551876, Length=446, Percent_Identity=34.9775784753363, Blast_Score=264, Evalue=8e-71,
Organism=Caenorhabditis elegans, GI71984122, Length=450, Percent_Identity=28.6666666666667, Blast_Score=172, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17556372, Length=411, Percent_Identity=25.5474452554745, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI32566633, Length=282, Percent_Identity=27.6595744680851, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI71994340, Length=336, Percent_Identity=23.5119047619048, Blast_Score=78, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17535925, Length=351, Percent_Identity=24.2165242165242, Blast_Score=78, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17535927, Length=337, Percent_Identity=24.0356083086053, Blast_Score=78, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6323011, Length=452, Percent_Identity=35.1769911504425, Blast_Score=270, Evalue=2e-73,
Organism=Saccharomyces cerevisiae, GI6321807, Length=448, Percent_Identity=28.3482142857143, Blast_Score=173, Evalue=4e-44,
Organism=Saccharomyces cerevisiae, GI6319872, Length=476, Percent_Identity=24.3697478991597, Blast_Score=130, Evalue=5e-31,
Organism=Saccharomyces cerevisiae, GI6325153, Length=264, Percent_Identity=28.4090909090909, Blast_Score=100, Evalue=6e-22,
Organism=Drosophila melanogaster, GI17136276, Length=458, Percent_Identity=33.4061135371179, Blast_Score=264, Evalue=1e-70,
Organism=Drosophila melanogaster, GI19921528, Length=438, Percent_Identity=29.9086757990868, Blast_Score=166, Evalue=2e-41,
Organism=Drosophila melanogaster, GI19922416, Length=460, Percent_Identity=22.8260869565217, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24584738, Length=328, Percent_Identity=27.1341463414634, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI24640851, Length=470, Percent_Identity=21.4893617021277, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24640849, Length=470, Percent_Identity=21.4893617021277, Blast_Score=75, Evalue=7e-14,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020780
- InterPro:   IPR004523
- InterPro:   IPR002312
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.12 [H]

Molecular weight: Translated: 49902; Mature: 49902

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRTLISELKNFMNEKINLQGWIHKIRKLRNITFLIIRDRSGLIQCIVDNDKLDLSQVKI
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCEEEEEEECCCCEEEEEECCCCCCHHHHHH
ESIVSLTGVVKESTNALNPFEIQVENLEIINAALDKLPIEINKENLEINLDTMLNNRILS
HHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHCCCEEECCCEEEEEEEHHCCCEEE
LRHPKVNSIFKVQNSIVNGFREFLNKEGFTEIYTPKIVWEGAEGGTEVFKVKYFENTAYL
ECCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEECCEEEEECCCCCCEEEEEEEECCCCHH
AQSPQFYKQMMVGAGFERVFEIGHAYRAEEHNTNRHLNEYISMDLEIGFIEDEKDIMEVE
HCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHEEEEEEEEECCHHHHHHHH
ERLLKFILEKLNKECKEYFDLLDSELPKIESEIPKITFKEALDILIKEYNKNNLTCDLDP
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCEEHHHHHHHHHHHCCCCCEEEEECC
EGEKLICKYAKEKLDSDFIFLTNYPREKRPMYTMPLGKDGTHSFDLLFRGIEITTGGQRI
CCHHHHHHHHHHHCCCCEEEEECCCCCCCCEEECCCCCCCCCCHHHEEECEEEECCCCEE
HDYNMLVDNMEFKGFNPKDYESYLSAFKYGMPKHGGLAIGLERLTSRLLGLENVREAALI
EEHHHHEECCCCCCCCCHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHCHHHHHHHHHH
TRDRTRLLP
CCCHHHCCC
>Mature Secondary Structure
MKRTLISELKNFMNEKINLQGWIHKIRKLRNITFLIIRDRSGLIQCIVDNDKLDLSQVKI
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCEEEEEEECCCCEEEEEECCCCCCHHHHHH
ESIVSLTGVVKESTNALNPFEIQVENLEIINAALDKLPIEINKENLEINLDTMLNNRILS
HHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHCCCEEECCCEEEEEEEHHCCCEEE
LRHPKVNSIFKVQNSIVNGFREFLNKEGFTEIYTPKIVWEGAEGGTEVFKVKYFENTAYL
ECCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEECCEEEEECCCCCCEEEEEEEECCCCHH
AQSPQFYKQMMVGAGFERVFEIGHAYRAEEHNTNRHLNEYISMDLEIGFIEDEKDIMEVE
HCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHEEEEEEEEECCHHHHHHHH
ERLLKFILEKLNKECKEYFDLLDSELPKIESEIPKITFKEALDILIKEYNKNNLTCDLDP
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCEEHHHHHHHHHHHCCCCCEEEEECC
EGEKLICKYAKEKLDSDFIFLTNYPREKRPMYTMPLGKDGTHSFDLLFRGIEITTGGQRI
CCHHHHHHHHHHHCCCCEEEEECCCCCCCCEEECCCCCCCCCCHHHEEECEEEECCCCEE
HDYNMLVDNMEFKGFNPKDYESYLSAFKYGMPKHGGLAIGLERLTSRLLGLENVREAALI
EEHHHHEECCCCCCCCCHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHCHHHHHHHHHH
TRDRTRLLP
CCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11930014 [H]