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The map label for this gene is dam [H]

Identifier: 148360693

GI number: 148360693

Start: 807543

End: 808361

Strand: Reverse

Name: dam [H]

Synonym: LPC_2640

Alternate gene names: 148360693

Gene position: 808361-807543 (Counterclockwise)

Preceding gene: 148360692

Following gene: 148360703

Centisome position: 22.6

GC content: 36.87

Gene sequence:

>819_bases
ATGGTAAAAATCAGACCTTTTCTGAAATGGGCAGGAAGTAAGTACAATTGTTTAAATGAAATTATCTCTTCTTTACCCAC
TGGCCAACGTTTAATAGAGCCATTTGCAGGTTCAGGCGTGGTATTTATGAACACCAGCTATTCATCTTACCTACTGGCTG
AAAGCAATCCTGATTTGGTTAATATTTATAGAATTCTACAATTGAATGGCGAATCCTTTATAGACTATTGCCAGCAATAC
TTTACCCCCGAAGCCAATTGCAAAGAAAAATATTATGAATTGCGAACTGAATTTAATAATTTAATGGATTCAAATCAAAA
ATCAGCGGTTTTTCTCTATCTTAATCGGCATGGATATAATGGTTTGTGCCGATACAATTCAAAAGGAAAATATAATGTCC
CTTTTGGTCTTTACGAAAAACCTTATTTTCCACGTAAGGAAATGATGCTGTTTTATAAAAAATGCCTTTCGGCACAGTTC
ATTCACGACGATTTTAGAAACACTTTTGCCTTAGCTGAACCCGGTGATGTCATTTACTGTGACCCACCCTATGTGCCACT
GACCGAAAATAATCAAACATTACCCTATAACCAAAAATTATTTTCTTTAGAAGATCAAATTGAGTTGGCTGAATTGGCCC
AGGAAACAGCCTCCAAAGGGATTCCTGTCATCATATCGAATCATGATACTGAATTTACGCGACATCATTACAAAAAAGCA
CAAATAAAGAGTTTCTATGTACCTCGCTGGATAAGCTGCAGAAGTCAGATGCGACTACCGGTTAAAGAGCTAGTCGCAGT
ATTCAACTGTCCATCCTGA

Upstream 100 bases:

>100_bases
GGGCTATTTTGTAAATCAGATAAGGAACCTTTATTCATTATCCTGTTCTTTATCCTGAAAATAAGAGATACTTCATCCTA
AAATATTGATTTTATAAGTT

Downstream 100 bases:

>100_bases
CAAAGACAAAGAAGAGTCTTTATCAAGATTAAGAGTTATTGGAATGGTCTTTGTCTTTAATAAAGAAAGCCAGAATAGTT
ACCAAAAGTAATGAAATAGG

Product: DNA adenine methylase

Products: NA

Alternate protein names: DNA adenine methyltransferase; Deoxyadenosyl-methyltransferase [H]

Number of amino acids: Translated: 272; Mature: 272

Protein sequence:

>272_residues
MVKIRPFLKWAGSKYNCLNEIISSLPTGQRLIEPFAGSGVVFMNTSYSSYLLAESNPDLVNIYRILQLNGESFIDYCQQY
FTPEANCKEKYYELRTEFNNLMDSNQKSAVFLYLNRHGYNGLCRYNSKGKYNVPFGLYEKPYFPRKEMMLFYKKCLSAQF
IHDDFRNTFALAEPGDVIYCDPPYVPLTENNQTLPYNQKLFSLEDQIELAELAQETASKGIPVIISNHDTEFTRHHYKKA
QIKSFYVPRWISCRSQMRLPVKELVAVFNCPS

Sequences:

>Translated_272_residues
MVKIRPFLKWAGSKYNCLNEIISSLPTGQRLIEPFAGSGVVFMNTSYSSYLLAESNPDLVNIYRILQLNGESFIDYCQQY
FTPEANCKEKYYELRTEFNNLMDSNQKSAVFLYLNRHGYNGLCRYNSKGKYNVPFGLYEKPYFPRKEMMLFYKKCLSAQF
IHDDFRNTFALAEPGDVIYCDPPYVPLTENNQTLPYNQKLFSLEDQIELAELAQETASKGIPVIISNHDTEFTRHHYKKA
QIKSFYVPRWISCRSQMRLPVKELVAVFNCPS
>Mature_272_residues
MVKIRPFLKWAGSKYNCLNEIISSLPTGQRLIEPFAGSGVVFMNTSYSSYLLAESNPDLVNIYRILQLNGESFIDYCQQY
FTPEANCKEKYYELRTEFNNLMDSNQKSAVFLYLNRHGYNGLCRYNSKGKYNVPFGLYEKPYFPRKEMMLFYKKCLSAQF
IHDDFRNTFALAEPGDVIYCDPPYVPLTENNQTLPYNQKLFSLEDQIELAELAQETASKGIPVIISNHDTEFTRHHYKKA
QIKSFYVPRWISCRSQMRLPVKELVAVFNCPS

Specific function: Methylates DNA within the sequence GATC and protects the DNA from cleavage by the restriction endonuclease MboI. Although it shares sequence specificity with a number of type II restriction endonucleases and methylases, it is thought to act in postreplica

COG id: COG0338

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789789, Length=268, Percent_Identity=45.5223880597015, Blast_Score=261, Evalue=4e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023095
- InterPro:   IPR002052
- InterPro:   IPR012327 [H]

Pfam domain/function: PF02086 MethyltransfD12 [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 31699; Mature: 31699

Theoretical pI: Translated: 8.17; Mature: 8.17

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKIRPFLKWAGSKYNCLNEIISSLPTGQRLIEPFAGSGVVFMNTSYSSYLLAESNPDLV
CCCCCHHHHHCCCCHHHHHHHHHHCCCCHHHHHHCCCCCEEEEECCCCEEEEECCCCCCE
NIYRILQLNGESFIDYCQQYFTPEANCKEKYYELRTEFNNLMDSNQKSAVFLYLNRHGYN
EEEHEEHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCC
GLCRYNSKGKYNVPFGLYEKPYFPRKEMMLFYKKCLSAQFIHDDFRNTFALAEPGDVIYC
CEEEECCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCEEEE
DPPYVPLTENNQTLPYNQKLFSLEDQIELAELAQETASKGIPVIISNHDTEFTRHHYKKA
CCCCEEECCCCCCCCCCCHHHCHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHH
QIKSFYVPRWISCRSQMRLPVKELVAVFNCPS
HHHHCCCCHHHHHHHHCCCCHHHHHHHHCCCC
>Mature Secondary Structure
MVKIRPFLKWAGSKYNCLNEIISSLPTGQRLIEPFAGSGVVFMNTSYSSYLLAESNPDLV
CCCCCHHHHHCCCCHHHHHHHHHHCCCCHHHHHHCCCCCEEEEECCCCEEEEECCCCCCE
NIYRILQLNGESFIDYCQQYFTPEANCKEKYYELRTEFNNLMDSNQKSAVFLYLNRHGYN
EEEHEEHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCC
GLCRYNSKGKYNVPFGLYEKPYFPRKEMMLFYKKCLSAQFIHDDFRNTFALAEPGDVIYC
CEEEECCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCEEEE
DPPYVPLTENNQTLPYNQKLFSLEDQIELAELAQETASKGIPVIISNHDTEFTRHHYKKA
CCCCEEECCCCCCCCCCCHHHCHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHH
QIKSFYVPRWISCRSQMRLPVKELVAVFNCPS
HHHHCCCCHHHHHHHHCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]