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The map label for this gene is irlS [H]

Identifier: 148360633

GI number: 148360633

Start: 888867

End: 889964

Strand: Reverse

Name: irlS [H]

Synonym: LPC_2579

Alternate gene names: 148360633

Gene position: 889964-888867 (Counterclockwise)

Preceding gene: 148360632

Following gene: 148360638

Centisome position: 24.88

GC content: 34.79

Gene sequence:

>1098_bases
ATGTATTGGAGATTCGCTAACTCAATGTCCATTACTAACCGCTTATTACTGATATTTATTGGTTCAACCGTACTGATATT
GTCCATTATCACTGGGTTAATCTATCCTCCAATGAAAGAATTACTCCATCATAGTCATTTGAATAATGAGCATTATAATT
ATTTATTAACACGGATTTGTATTAAAAATTTCTTTGTTGGACTCTGGTTTTCTACTTTGATAATCATTCTTGCCAGTTAT
TTGCTCGCAAAAAAAAGCATGAGACCAATTAAAATATTCACTAAAGAGCTTGCATCGATTAATGCCTCATCACTAGAGAA
ACGATTACCTTACACAGGGAATCCCAAAGAACTGGAAGAATTAGCTTTGACTTGTAATGATATGCTCTTTAGAATAGAAA
GTGCATTTAGCCATATAAAGCAATTTTCAGCCAGTATAGCTCATGAATTACGAAATCCCATTCATTACCTCCGAACTGCT
ACCGAAATTACCCTGACTAAACCACAAACGATTGAAACCTACCAACATGTATTACAAACGCATCTCGAAGAATATCAAAG
TTTAACCCAACTTATAGATAATCTGCTCTTTTTAACTCGCTGTGAGCACGGCCAACTTCAATTAAATGTTAGAACATTAT
CTGCAAACCACTTAATCAGCTCAATTATCGAGTATTATTATTCAATAGCTCAAGAAAAGGGCATAGAAATTCAGATGTTT
GGCGAGGCTCAGATTGAAGTAGATGAACATTTATTTAAGCGCGTTATTTCAAATTTAATAGACAACAGTCTGACCTATAC
CAACAAGGGCGGAAGAATACTAATCATCATAGAAAAAAGAACAGACAACAGCATTCAAATCCGTATAAAAGACAATGGAA
TTGGTATATCAAAAGAACACTTACCTCTTTTATATCACGGATTTTATCGCGTAGATTATAGCACTAACAAAGAAAACGCA
GGTCTTGGTTTAGGTTTGGCTATCGCTAAATCAATCATGGAACACCATAAAGGCCAGCTTGCGATAGACAGCAAGATGGG
TTTAGGCACCACTGTTATATTAACCCTACCAGGAAAAAGCTTTTTAAAGATTGAATAA

Upstream 100 bases:

>100_bases
ACGAATACCAATATCATTGATGTAGCGATCAAGCGTTTAAGGGAAAAAGTGGATAATGATCATCCTACTAAATTGATCCA
TACCGTCAGAGGAGTAGGCT

Downstream 100 bases:

>100_bases
TGTATGTATTTCATCTATCCGAGACCTTAAGGGAACGGATATACATTGAACTAAGTTATTCTAAGAACATAGACTTTTCC
CGCCTCACACAGCAACACCA

Product: sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MYWRFANSMSITNRLLLIFIGSTVLILSIITGLIYPPMKELLHHSHLNNEHYNYLLTRICIKNFFVGLWFSTLIIILASY
LLAKKSMRPIKIFTKELASINASSLEKRLPYTGNPKELEELALTCNDMLFRIESAFSHIKQFSASIAHELRNPIHYLRTA
TEITLTKPQTIETYQHVLQTHLEEYQSLTQLIDNLLFLTRCEHGQLQLNVRTLSANHLISSIIEYYYSIAQEKGIEIQMF
GEAQIEVDEHLFKRVISNLIDNSLTYTNKGGRILIIIEKRTDNSIQIRIKDNGIGISKEHLPLLYHGFYRVDYSTNKENA
GLGLGLAIAKSIMEHHKGQLAIDSKMGLGTTVILTLPGKSFLKIE

Sequences:

>Translated_365_residues
MYWRFANSMSITNRLLLIFIGSTVLILSIITGLIYPPMKELLHHSHLNNEHYNYLLTRICIKNFFVGLWFSTLIIILASY
LLAKKSMRPIKIFTKELASINASSLEKRLPYTGNPKELEELALTCNDMLFRIESAFSHIKQFSASIAHELRNPIHYLRTA
TEITLTKPQTIETYQHVLQTHLEEYQSLTQLIDNLLFLTRCEHGQLQLNVRTLSANHLISSIIEYYYSIAQEKGIEIQMF
GEAQIEVDEHLFKRVISNLIDNSLTYTNKGGRILIIIEKRTDNSIQIRIKDNGIGISKEHLPLLYHGFYRVDYSTNKENA
GLGLGLAIAKSIMEHHKGQLAIDSKMGLGTTVILTLPGKSFLKIE
>Mature_365_residues
MYWRFANSMSITNRLLLIFIGSTVLILSIITGLIYPPMKELLHHSHLNNEHYNYLLTRICIKNFFVGLWFSTLIIILASY
LLAKKSMRPIKIFTKELASINASSLEKRLPYTGNPKELEELALTCNDMLFRIESAFSHIKQFSASIAHELRNPIHYLRTA
TEITLTKPQTIETYQHVLQTHLEEYQSLTQLIDNLLFLTRCEHGQLQLNVRTLSANHLISSIIEYYYSIAQEKGIEIQMF
GEAQIEVDEHLFKRVISNLIDNSLTYTNKGGRILIIIEKRTDNSIQIRIKDNGIGISKEHLPLLYHGFYRVDYSTNKENA
GLGLGLAIAKSIMEHHKGQLAIDSKMGLGTTVILTLPGKSFLKIE

Specific function: Member of the two-component regulatory system irlR/irlS. May be involved in invasion of eukaryotic cells and heavy-metal resistance. Probably activates irlR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1786783, Length=288, Percent_Identity=34.0277777777778, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI1788279, Length=260, Percent_Identity=30, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1786600, Length=285, Percent_Identity=27.0175438596491, Blast_Score=108, Evalue=6e-25,
Organism=Escherichia coli, GI1788393, Length=277, Percent_Identity=28.5198555956679, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI145693157, Length=233, Percent_Identity=27.0386266094421, Blast_Score=98, Evalue=7e-22,
Organism=Escherichia coli, GI1790346, Length=277, Percent_Identity=25.9927797833935, Blast_Score=95, Evalue=7e-21,
Organism=Escherichia coli, GI1788713, Length=228, Percent_Identity=30.7017543859649, Blast_Score=95, Evalue=7e-21,
Organism=Escherichia coli, GI1789149, Length=229, Percent_Identity=26.2008733624454, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1786912, Length=265, Percent_Identity=29.0566037735849, Blast_Score=85, Evalue=8e-18,
Organism=Escherichia coli, GI1788549, Length=229, Percent_Identity=28.3842794759825, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1789403, Length=264, Percent_Identity=27.2727272727273, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI48994928, Length=234, Percent_Identity=22.6495726495727, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1790551, Length=308, Percent_Identity=23.7012987012987, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787894, Length=243, Percent_Identity=25.5144032921811, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1790436, Length=220, Percent_Identity=25.4545454545455, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI1790861, Length=219, Percent_Identity=24.6575342465753, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1787374, Length=292, Percent_Identity=22.6027397260274, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI87081816, Length=248, Percent_Identity=22.9838709677419, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789808, Length=245, Percent_Identity=26.9387755102041, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR006290
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 41776; Mature: 41776

Theoretical pI: Translated: 8.90; Mature: 8.90

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYWRFANSMSITNRLLLIFIGSTVLILSIITGLIYPPMKELLHHSHLNNEHYNYLLTRIC
CCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHH
IKNFFVGLWFSTLIIILASYLLAKKSMRPIKIFTKELASINASSLEKRLPYTGNPKELEE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHCCCCCCCHHHHHH
LALTCNDMLFRIESAFSHIKQFSASIAHELRNPIHYLRTATEITLTKPQTIETYQHVLQT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCHHHHHHHHHHH
HLEEYQSLTQLIDNLLFLTRCEHGQLQLNVRTLSANHLISSIIEYYYSIAQEKGIEIQMF
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECHHHHHHHHHHHHHHHHHHCCCEEEEE
GEAQIEVDEHLFKRVISNLIDNSLTYTNKGGRILIIIEKRTDNSIQIRIKDNGIGISKEH
CCCEEHHHHHHHHHHHHHHHCCCCEEECCCCEEEEEEEECCCCEEEEEEECCCCCCCCCC
LPLLYHGFYRVDYSTNKENAGLGLGLAIAKSIMEHHKGQLAIDSKMGLGTTVILTLPGKS
CCEEEECEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCEEEEEEECCCC
FLKIE
EEEEC
>Mature Secondary Structure
MYWRFANSMSITNRLLLIFIGSTVLILSIITGLIYPPMKELLHHSHLNNEHYNYLLTRIC
CCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHH
IKNFFVGLWFSTLIIILASYLLAKKSMRPIKIFTKELASINASSLEKRLPYTGNPKELEE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHCCCCCCCHHHHHH
LALTCNDMLFRIESAFSHIKQFSASIAHELRNPIHYLRTATEITLTKPQTIETYQHVLQT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCHHHHHHHHHHH
HLEEYQSLTQLIDNLLFLTRCEHGQLQLNVRTLSANHLISSIIEYYYSIAQEKGIEIQMF
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECHHHHHHHHHHHHHHHHHHCCCEEEEE
GEAQIEVDEHLFKRVISNLIDNSLTYTNKGGRILIIIEKRTDNSIQIRIKDNGIGISKEH
CCCEEHHHHHHHHHHHHHHHCCCCEEECCCCEEEEEEEECCCCEEEEEEECCCCCCCCCC
LPLLYHGFYRVDYSTNKENAGLGLGLAIAKSIMEHHKGQLAIDSKMGLGTTVILTLPGKS
CCEEEECEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCEEEEEEECCCC
FLKIE
EEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9393784 [H]