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The map label for this gene is birA [H]

Identifier: 148360565

GI number: 148360565

Start: 965188

End: 966183

Strand: Reverse

Name: birA [H]

Synonym: LPC_2509

Alternate gene names: 148360565

Gene position: 966183-965188 (Counterclockwise)

Preceding gene: 148360564

Following gene: 148360566

Centisome position: 27.01

GC content: 36.45

Gene sequence:

>996_bases
ATGTTTCAATTTAATACTTCTCAACATACGTTAATGCAAATACTGGGCGATGGGGCATGTCATAGTGGGAGTGAATTAGG
AAACGCTTTAAAAATCAGTCGCTCAGCAGTATGGAAACAAATCAATCAGCTTAATGATTTAGGCATACCTATTATCCGCA
TACCACATCAAGGATACCAATTGGAAAAGCCTTTAATCCTGCTAGACTCCAATGAAATTACCAAGCAATTACACAGCAAA
GGATTTAGCCAACCGTTTAACCTGCATTTATTTACATCGATTGACTCAACCAACCGCTACCTGAAAGATCTGCCTTCAAG
CTCAGCCGTCGAAATTTGCTGCACTGAAATGCAAACTCAAGGGAGAGGGCGCTTTGGACGACACTGGCACTCTCCTTTTG
GAGAGAATATTTACTGCTCTACCCGGTGGAGTTTTAATTGTGACATATCTAAGCTATCGGGTCTCAGTTTAGTCATAAGT
TTAGCAACAGTTCTCACTATAAATGAATTCATTGTATCTGATAAAGTAAAAATAAAATGGCCCAATGATATCCTGTGGAA
TAATAAAAAGTTATGTGGGATTTTAGTAGAGATTATTGCAGAATCTAACGCGACAGCACAATTTATTATAGGTGTTGGTT
TAAATATCAACTCTGATACAAAAAACAATCCTCTACCAGATAAATCCTGGAGTTCTTTTTTTGGAATAACTAATCAAAAT
TACAATCGGAATTCGATTGTTACAAGGCTATTAATTAACCTGGAAAAATATCTATCAAAATTTATTAATAACGATCTAAA
TGCATTTAAGGATGACTGGAATAAGTATGACTACCTTGTTGGGAAAAAAGTTAAAGTGTCCCAAGCTTTGAATTCGATTT
CCGGGATTGCCTTGGGCATTAATTCTTTTGGCCAACTGATTCTGGAAGATGAATTTGGAAATAAGCATGAACTCTCTTCG
GGTGATACATCACTTGCCCTAAGTAATTTTGAATAG

Upstream 100 bases:

>100_bases
AGTATGCTGCAGTGGCGCTTTATGCTATCACCCTTCTGTTAGAGAAATTCGTCATGGGATCGTCCAGCTTCAACAAAATA
TTAGTTTAAAATTTTAAATC

Downstream 100 bases:

>100_bases
GGTTTTTAACAAAAAGGAAGTTTTAGAGTAAAATTTCAAATCTAATATTTTAATAACTTCACTTTATTCTATAATCGCTT
TTCACGCAACATGTACAAAA

Product: bifunctional biotin operon repressor/biotin [acetyl CoA carboxylase] synthetase BirA

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MFQFNTSQHTLMQILGDGACHSGSELGNALKISRSAVWKQINQLNDLGIPIIRIPHQGYQLEKPLILLDSNEITKQLHSK
GFSQPFNLHLFTSIDSTNRYLKDLPSSSAVEICCTEMQTQGRGRFGRHWHSPFGENIYCSTRWSFNCDISKLSGLSLVIS
LATVLTINEFIVSDKVKIKWPNDILWNNKKLCGILVEIIAESNATAQFIIGVGLNINSDTKNNPLPDKSWSSFFGITNQN
YNRNSIVTRLLINLEKYLSKFINNDLNAFKDDWNKYDYLVGKKVKVSQALNSISGIALGINSFGQLILEDEFGNKHELSS
GDTSLALSNFE

Sequences:

>Translated_331_residues
MFQFNTSQHTLMQILGDGACHSGSELGNALKISRSAVWKQINQLNDLGIPIIRIPHQGYQLEKPLILLDSNEITKQLHSK
GFSQPFNLHLFTSIDSTNRYLKDLPSSSAVEICCTEMQTQGRGRFGRHWHSPFGENIYCSTRWSFNCDISKLSGLSLVIS
LATVLTINEFIVSDKVKIKWPNDILWNNKKLCGILVEIIAESNATAQFIIGVGLNINSDTKNNPLPDKSWSSFFGITNQN
YNRNSIVTRLLINLEKYLSKFINNDLNAFKDDWNKYDYLVGKKVKVSQALNSISGIALGINSFGQLILEDEFGNKHELSS
GDTSLALSNFE
>Mature_331_residues
MFQFNTSQHTLMQILGDGACHSGSELGNALKISRSAVWKQINQLNDLGIPIIRIPHQGYQLEKPLILLDSNEITKQLHSK
GFSQPFNLHLFTSIDSTNRYLKDLPSSSAVEICCTEMQTQGRGRFGRHWHSPFGENIYCSTRWSFNCDISKLSGLSLVIS
LATVLTINEFIVSDKVKIKWPNDILWNNKKLCGILVEIIAESNATAQFIIGVGLNINSDTKNNPLPDKSWSSFFGITNQN
YNRNSIVTRLLINLEKYLSKFINNDLNAFKDDWNKYDYLVGKKVKVSQALNSISGIALGINSFGQLILEDEFGNKHELSS
GDTSLALSNFE

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=310, Percent_Identity=34.5161290322581, Blast_Score=187, Evalue=7e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004408
- InterPro:   IPR004409
- InterPro:   IPR003142
- InterPro:   IPR004143
- InterPro:   IPR013196
- InterPro:   IPR008988
- InterPro:   IPR011991 [H]

Pfam domain/function: PF02237 BPL_C; PF03099 BPL_LipA_LipB; PF08279 HTH_11 [H]

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 37154; Mature: 37154

Theoretical pI: Translated: 7.86; Mature: 7.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFQFNTSQHTLMQILGDGACHSGSELGNALKISRSAVWKQINQLNDLGIPIIRIPHQGYQ
CCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCC
LEKPLILLDSNEITKQLHSKGFSQPFNLHLFTSIDSTNRYLKDLPSSSAVEICCTEMQTQ
CCCCEEEECCHHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHCCCCCHHHHHHHHHHHC
GRGRFGRHWHSPFGENIYCSTRWSFNCDISKLSGLSLVISLATVLTINEFIVSDKVKIKW
CCCCCCCCCCCCCCCCEEEEECEEECCCHHHHCHHHHHHHHHHHHHHHHHHHCCEEEEEC
PNDILWNNKKLCGILVEIIAESNATAQFIIGVGLNINSDTKNNPLPDKSWSSFFGITNQN
CCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEECEEECCCCCCCCCCCCCCHHHCCCCCCC
YNRNSIVTRLLINLEKYLSKFINNDLNAFKDDWNKYDYLVGKKVKVSQALNSISGIALGI
CCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHEEEEH
NSFGQLILEDEFGNKHELSSGDTSLALSNFE
HHHCCEEEECCCCCCCCCCCCCCEEEECCCC
>Mature Secondary Structure
MFQFNTSQHTLMQILGDGACHSGSELGNALKISRSAVWKQINQLNDLGIPIIRIPHQGYQ
CCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCC
LEKPLILLDSNEITKQLHSKGFSQPFNLHLFTSIDSTNRYLKDLPSSSAVEICCTEMQTQ
CCCCEEEECCHHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHCCCCCHHHHHHHHHHHC
GRGRFGRHWHSPFGENIYCSTRWSFNCDISKLSGLSLVISLATVLTINEFIVSDKVKIKW
CCCCCCCCCCCCCCCCEEEEECEEECCCHHHHCHHHHHHHHHHHHHHHHHHHCCEEEEEC
PNDILWNNKKLCGILVEIIAESNATAQFIIGVGLNINSDTKNNPLPDKSWSSFFGITNQN
CCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEECEEECCCCCCCCCCCCCCHHHCCCCCCC
YNRNSIVTRLLINLEKYLSKFINNDLNAFKDDWNKYDYLVGKKVKVSQALNSISGIALGI
CCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHEEEEH
NSFGQLILEDEFGNKHELSSGDTSLALSNFE
HHHCCEEEECCCCCCCCCCCCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3899863; 3536662; 8265357; 9278503; 1311302; 1328157; 1409631; 11353844 [H]