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The map label for this gene is proP6 [H]

Identifier: 148360274

GI number: 148360274

Start: 1275057

End: 1276349

Strand: Reverse

Name: proP6 [H]

Synonym: LPC_2211

Alternate gene names: 148360274

Gene position: 1276349-1275057 (Counterclockwise)

Preceding gene: 148360273

Following gene: 148360276

Centisome position: 35.69

GC content: 36.81

Gene sequence:

>1293_bases
ATGAAGCACCAAACCCGAATCATTCTGGCAGGTACACTGGGTAATTTAATTGAATCCTTTGACATGGCTATTTGTGGCCT
ACTCTCAGTTTACATTGCCAAATACCTGATTGGTGATGTCTCCAAGGGTTTGTTTCTCGTCTTTCTTACTTTCTTTGCTG
GATACCTAGCCAGACCTATTGGGGCTATGATAATGGGCTTACTATCCGATATCTATGGCAGAAAAATTATCCTTGCAGGT
TCTATCCTAACAATGGGCATATCAACAACCCTTATCGGGTTTATCCCCCCTCACAGTACTATAGGTACATTTTCTGCTAT
TACCTTATTAGTGTTAAGAATTATTCAGAGCTTCTCCTGCGGTGCAGAATATTTAAACTCCTCTGCTTACCTAGTTGAGA
ATGCCGAAGCCTCAAAAAAGGGCTACTCAGGAAGCTGGGCATCCTTTGGCGCTATGTCAGGGATGCTGATAGCGTCTTTA
GTTGTATTGATTGTTACTTATTTTACCAGTCACTATCCTGAACAGGACTGGATGATCTGGCGTGTTCCTTTTGTGCTTGC
GCTTTTAGGATCTTCGATTGGTTTATACATCCGTTTATGTATCCCCGAGAGTATGGAATACATCGTATACTATGCAGACA
GACCAAAGCCTAAATTTAAGAGTTTACTTTCTGAATCGGTAACCTATATCAAAAACAATAAAATACAATCCCTTTATGTG
TTTATTTTGAGTTGTTTGGGTGTAACAACTACATTTCAAATTTACATTTATGGTCCTATGCAAGCTCGTTTATATGGTAA
TTTTCAAAACCATGAAATTATCATTTCCAACATCATTTCTTTAATGGTACTTCTAGGAGTATTTCCTCTGATTGGGAATC
TATCTGATAAAATTAGTAGAGAAAAAATCGTAGTTGCAGCAAGTATAGGATTTCTAGTTCTATCTCAACCTTTTTTCAAC
TTCTTATCGCACAAAATATTTTATCAGTTAATTTTGAGTCAAGCTTTAATTGCTATTCCAGCAGGAGCATATTATGCAAC
AGTACCAGTAATGTTAGCTGAAATGTTCCCGATTAAATTACGTTGTAGTGTTTTATCAATACTATATTCCATTGCAGCAA
GTTTATCCGCTGGCCTCGCTCCATTACTTTCTATGATACTTGTAAAAAAAACCGGCGTTGCATCGTCACCATCAATTTTA
GTTTTTACGCTAATAGCAACAATGTTTATAATAATAATTTACAGAACCATAAAACCTGAAAAGAGATGCTCTTTAACGAC
ACTTAAAGGCTAA

Upstream 100 bases:

>100_bases
CATGAAATTGATCACCTGGATGGATTTTTATTTTTAGACCGAGTGGAAGATCAAGATTCACTAACCACTCTATCGGCAAT
GCAAAGTAAAGGATAATCGC

Downstream 100 bases:

>100_bases
AATAATAACATCGCATGGCAATGTGCTTCGGATAAAAGTACCGCTCAGATTACTTAATGATAGTGTTGCAAAAATATTCC
CAATAAATGAGGCAGATTCT

Product: proline/betaine transporter ProP6

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MKHQTRIILAGTLGNLIESFDMAICGLLSVYIAKYLIGDVSKGLFLVFLTFFAGYLARPIGAMIMGLLSDIYGRKIILAG
SILTMGISTTLIGFIPPHSTIGTFSAITLLVLRIIQSFSCGAEYLNSSAYLVENAEASKKGYSGSWASFGAMSGMLIASL
VVLIVTYFTSHYPEQDWMIWRVPFVLALLGSSIGLYIRLCIPESMEYIVYYADRPKPKFKSLLSESVTYIKNNKIQSLYV
FILSCLGVTTTFQIYIYGPMQARLYGNFQNHEIIISNIISLMVLLGVFPLIGNLSDKISREKIVVAASIGFLVLSQPFFN
FLSHKIFYQLILSQALIAIPAGAYYATVPVMLAEMFPIKLRCSVLSILYSIAASLSAGLAPLLSMILVKKTGVASSPSIL
VFTLIATMFIIIIYRTIKPEKRCSLTTLKG

Sequences:

>Translated_430_residues
MKHQTRIILAGTLGNLIESFDMAICGLLSVYIAKYLIGDVSKGLFLVFLTFFAGYLARPIGAMIMGLLSDIYGRKIILAG
SILTMGISTTLIGFIPPHSTIGTFSAITLLVLRIIQSFSCGAEYLNSSAYLVENAEASKKGYSGSWASFGAMSGMLIASL
VVLIVTYFTSHYPEQDWMIWRVPFVLALLGSSIGLYIRLCIPESMEYIVYYADRPKPKFKSLLSESVTYIKNNKIQSLYV
FILSCLGVTTTFQIYIYGPMQARLYGNFQNHEIIISNIISLMVLLGVFPLIGNLSDKISREKIVVAASIGFLVLSQPFFN
FLSHKIFYQLILSQALIAIPAGAYYATVPVMLAEMFPIKLRCSVLSILYSIAASLSAGLAPLLSMILVKKTGVASSPSIL
VFTLIATMFIIIIYRTIKPEKRCSLTTLKG
>Mature_430_residues
MKHQTRIILAGTLGNLIESFDMAICGLLSVYIAKYLIGDVSKGLFLVFLTFFAGYLARPIGAMIMGLLSDIYGRKIILAG
SILTMGISTTLIGFIPPHSTIGTFSAITLLVLRIIQSFSCGAEYLNSSAYLVENAEASKKGYSGSWASFGAMSGMLIASL
VVLIVTYFTSHYPEQDWMIWRVPFVLALLGSSIGLYIRLCIPESMEYIVYYADRPKPKFKSLLSESVTYIKNNKIQSLYV
FILSCLGVTTTFQIYIYGPMQARLYGNFQNHEIIISNIISLMVLLGVFPLIGNLSDKISREKIVVAASIGFLVLSQPFFN
FLSHKIFYQLILSQALIAIPAGAYYATVPVMLAEMFPIKLRCSVLSILYSIAASLSAGLAPLLSMILVKKTGVASSPSIL
VFTLIATMFIIIIYRTIKPEKRCSLTTLKG

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=395, Percent_Identity=30.126582278481, Blast_Score=174, Evalue=1e-44,
Organism=Escherichia coli, GI1788292, Length=397, Percent_Identity=26.1964735516373, Blast_Score=118, Evalue=7e-28,
Organism=Escherichia coli, GI1788942, Length=395, Percent_Identity=26.8354430379747, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1789941, Length=406, Percent_Identity=26.6009852216749, Blast_Score=108, Evalue=6e-25,
Organism=Escherichia coli, GI87082404, Length=396, Percent_Identity=26.5151515151515, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 47280; Mature: 47280

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHQTRIILAGTLGNLIESFDMAICGLLSVYIAKYLIGDVSKGLFLVFLTFFAGYLARPI
CCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GAMIMGLLSDIYGRKIILAGSILTMGISTTLIGFIPPHSTIGTFSAITLLVLRIIQSFSC
HHHHHHHHHHHHCCEEEEECHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHCC
GAEYLNSSAYLVENAEASKKGYSGSWASFGAMSGMLIASLVVLIVTYFTSHYPEQDWMIW
CHHHHCCCEEEEECCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEE
RVPFVLALLGSSIGLYIRLCIPESMEYIVYYADRPKPKFKSLLSESVTYIKNNKIQSLYV
HHHHHHHHHHCCCCEEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHHH
FILSCLGVTTTFQIYIYGPMQARLYGNFQNHEIIISNIISLMVLLGVFPLIGNLSDKISR
HHHHHHCCCEEEEEEEEECEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHCC
EKIVVAASIGFLVLSQPFFNFLSHKIFYQLILSQALIAIPAGAYYATVPVMLAEMFPIKL
CCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHH
RCSVLSILYSIAASLSAGLAPLLSMILVKKTGVASSPSILVFTLIATMFIIIIYRTIKPE
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
KRCSLTTLKG
CCCCCCCCCC
>Mature Secondary Structure
MKHQTRIILAGTLGNLIESFDMAICGLLSVYIAKYLIGDVSKGLFLVFLTFFAGYLARPI
CCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GAMIMGLLSDIYGRKIILAGSILTMGISTTLIGFIPPHSTIGTFSAITLLVLRIIQSFSC
HHHHHHHHHHHHCCEEEEECHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHCC
GAEYLNSSAYLVENAEASKKGYSGSWASFGAMSGMLIASLVVLIVTYFTSHYPEQDWMIW
CHHHHCCCEEEEECCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEE
RVPFVLALLGSSIGLYIRLCIPESMEYIVYYADRPKPKFKSLLSESVTYIKNNKIQSLYV
HHHHHHHHHHCCCCEEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHHH
FILSCLGVTTTFQIYIYGPMQARLYGNFQNHEIIISNIISLMVLLGVFPLIGNLSDKISR
HHHHHHCCCEEEEEEEEECEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHCC
EKIVVAASIGFLVLSQPFFNFLSHKIFYQLILSQALIAIPAGAYYATVPVMLAEMFPIKL
CCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHH
RCSVLSILYSIAASLSAGLAPLLSMILVKKTGVASSPSILVFTLIATMFIIIIYRTIKPE
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
KRCSLTTLKG
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]