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The map label for this gene is def1 [H]

Identifier: 148360273

GI number: 148360273

Start: 1276354

End: 1276872

Strand: Reverse

Name: def1 [H]

Synonym: LPC_2210

Alternate gene names: 148360273

Gene position: 1276872-1276354 (Counterclockwise)

Preceding gene: 148360272

Following gene: 148360274

Centisome position: 35.7

GC content: 38.34

Gene sequence:

>519_bases
ATGAACACACTCCTTGATAAAAACAACCCTATTTTAAGACAAACCGCTGATCCAATTTCTGAATCAGAATTTGGTAGCAG
TTGGTTAAAAGAGCTTATTAAGACTATGTTTGGCATAATGGCTGACAAGGGGGCTGTAGGCGTAGCTGCCCCCCAAATTG
GCATCAGCAAACGAGTTATTGTATTTGGTACTGATTACACCAAAAGGAGAAAACCAGAATACCCAATTCCTGATACAGCC
TTAATTAATCCTTCATTAAAAATCCTATCTCAAGAAATTCAAACTGGCTACGAGGGTTGCCTTAATTGTGGTGAATTAAT
GGGTGAAGTTCCAAGAGCCATGGAAATTGAATATTCAGGGTTTGACATAGATGGAAATAGAATTACCAAAAAAGCTTCCG
GTTTAGAAGCCCGCATTCTTCAACATGAAATTGATCACCTGGATGGATTTTTATTTTTAGACCGAGTGGAAGATCAAGAT
TCACTAACCACTCTATCGGCAATGCAAAGTAAAGGATAA

Upstream 100 bases:

>100_bases
GCGTTAAGGCGAATTCAAGAGTATTTAATTTTCCCTGACAAACAGCCCTGCATGACGACAAAACCCAAACTGACATTAAT
CATCAACAATCAGGTGCCCC

Downstream 100 bases:

>100_bases
TCGCATGAAGCACCAAACCCGAATCATTCTGGCAGGTACACTGGGTAATTTAATTGAATCCTTTGACATGGCTATTTGTG
GCCTACTCTCAGTTTACATT

Product: polypeptide deformylase

Products: NA

Alternate protein names: PDF 1; Polypeptide deformylase 1 [H]

Number of amino acids: Translated: 172; Mature: 172

Protein sequence:

>172_residues
MNTLLDKNNPILRQTADPISESEFGSSWLKELIKTMFGIMADKGAVGVAAPQIGISKRVIVFGTDYTKRRKPEYPIPDTA
LINPSLKILSQEIQTGYEGCLNCGELMGEVPRAMEIEYSGFDIDGNRITKKASGLEARILQHEIDHLDGFLFLDRVEDQD
SLTTLSAMQSKG

Sequences:

>Translated_172_residues
MNTLLDKNNPILRQTADPISESEFGSSWLKELIKTMFGIMADKGAVGVAAPQIGISKRVIVFGTDYTKRRKPEYPIPDTA
LINPSLKILSQEIQTGYEGCLNCGELMGEVPRAMEIEYSGFDIDGNRITKKASGLEARILQHEIDHLDGFLFLDRVEDQD
SLTTLSAMQSKG
>Mature_172_residues
MNTLLDKNNPILRQTADPISESEFGSSWLKELIKTMFGIMADKGAVGVAAPQIGISKRVIVFGTDYTKRRKPEYPIPDTA
LINPSLKILSQEIQTGYEGCLNCGELMGEVPRAMEIEYSGFDIDGNRITKKASGLEARILQHEIDHLDGFLFLDRVEDQD
SLTTLSAMQSKG

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=171, Percent_Identity=32.1637426900585, Blast_Score=107, Evalue=4e-24,
Organism=Escherichia coli, GI1789682, Length=144, Percent_Identity=34.7222222222222, Blast_Score=79, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24645728, Length=158, Percent_Identity=32.9113924050633, Blast_Score=90, Evalue=6e-19,
Organism=Drosophila melanogaster, GI24645726, Length=183, Percent_Identity=30.6010928961749, Blast_Score=89, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 19037; Mature: 19037

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTLLDKNNPILRQTADPISESEFGSSWLKELIKTMFGIMADKGAVGVAAPQIGISKRVI
CCCCCCCCCCCHHCCCCCCCHHHCCHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCEEE
VFGTDYTKRRKPEYPIPDTALINPSLKILSQEIQTGYEGCLNCGELMGEVPRAMEIEYSG
EEECCCHHCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECC
FDIDGNRITKKASGLEARILQHEIDHLDGFLFLDRVEDQDSLTTLSAMQSKG
EECCCCHHHHHHCCHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MNTLLDKNNPILRQTADPISESEFGSSWLKELIKTMFGIMADKGAVGVAAPQIGISKRVI
CCCCCCCCCCCHHCCCCCCCHHHCCHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCEEE
VFGTDYTKRRKPEYPIPDTALINPSLKILSQEIQTGYEGCLNCGELMGEVPRAMEIEYSG
EEECCCHHCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECC
FDIDGNRITKKASGLEARILQHEIDHLDGFLFLDRVEDQDSLTTLSAMQSKG
EECCCCHHHHHHCCHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12910271 [H]