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The map label for this gene is insI1 [H]

Identifier: 148360254

GI number: 148360254

Start: 1295115

End: 1296101

Strand: Reverse

Name: insI1 [H]

Synonym: LPC_2189

Alternate gene names: 148360254

Gene position: 1296101-1295115 (Counterclockwise)

Preceding gene: 148360252

Following gene: 148360255

Centisome position: 36.24

GC content: 34.55

Gene sequence:

>987_bases
ATGGGACAGTATACTCAATTATCTGTAACAGATCGACGCCGTTTATTTGTTTTTCTGGATATGGGATTATCTGTACGCGA
GATCACGCATAAATTAAAGAGGCATCGTTCAACAATATACAGAGAACTAAATAGGAATAATGAATCGGGAGTTTATTTAC
CGATTATAGCCCATCAGAAGGCTTTAGCTAGAGCTCAAAATAATCGCCCTAATAAACTAAAAAATAATGGAATTTTACGT
GATTATGTTGTTCGGTCATTAGTCAATAAAGGATGGAGTCCCGAACAGATATCTGGCCGGATGAAATATCAAAAATTGAG
TTTTTATGTTTGTCCTGAAACTATTTATCGTTATGTTTATCAATCTAATGATAAGGCGCTTTATCATTCACTAGCCTTTA
AAAAACCTAGAAGACAAAAGCTCTTTAGGCGAAAGCATAGAACATGTCGTTATGGCACTCCTTATCTGATTACTCAAAGA
CCTGAAGAAATCGATTCAAGGGAACGTTTTGGACACTGGGAAGGCGATACAATTGAATTTAAAGGGAATAAAAGCGAAGT
GATTACGACACTTGTGGAGCGTAAAACCAGAATGGTTTATCTCATAAAAAATACGAGCAAACATTCACGAGGAGTGATGG
AAAAAATAAATAAGAAATTTGAAAAATTACCACAAAAAATGTTTAAAACAATCACTTTTGATCAGGGTTCTGAGTTTGCT
GCTCATGAATATCTTGAGAAAAACATGAAATGTGAAGTTTATTATTGTGATGTTCACTCCCCATGGCAAAAAGGAAGTAA
TGAAAATATGAATGGTCGAATAAGAAAATACTTACCTAAAAATGCTAAAATTAACTTAATAACCCAAGAGGAGTTGGACT
TAATAGCAGCTAAATTGAACCGGTGCCCTAGAAAATGTATCGGTTATAAAACGCCCAACGAACTGTTTATACAGCAGTAT
AAAAATGATTGTCGCACTTGGAGTTAA

Upstream 100 bases:

>100_bases
GCAATCAAAAACAAGTTGGGGAATTCTAATCTGAAGTGCGACAGTCCAAAAAGCTGTATCATGTTCTTGGGGAACTTGAA
ATAGCAAAGGAGACTGCCAC

Downstream 100 bases:

>100_bases
AATTCGCCCCCGAAGTTTTTTAATGTAACCAGTTGATTTTAATGGTGCCGTTGCGCGGAATCGAACCGCGGACCTATTGA
TTACGAATTAGTGAATTATG

Product: IS30B/C/D transposase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 327

Protein sequence:

>328_residues
MGQYTQLSVTDRRRLFVFLDMGLSVREITHKLKRHRSTIYRELNRNNESGVYLPIIAHQKALARAQNNRPNKLKNNGILR
DYVVRSLVNKGWSPEQISGRMKYQKLSFYVCPETIYRYVYQSNDKALYHSLAFKKPRRQKLFRRKHRTCRYGTPYLITQR
PEEIDSRERFGHWEGDTIEFKGNKSEVITTLVERKTRMVYLIKNTSKHSRGVMEKINKKFEKLPQKMFKTITFDQGSEFA
AHEYLEKNMKCEVYYCDVHSPWQKGSNENMNGRIRKYLPKNAKINLITQEELDLIAAKLNRCPRKCIGYKTPNELFIQQY
KNDCRTWS

Sequences:

>Translated_328_residues
MGQYTQLSVTDRRRLFVFLDMGLSVREITHKLKRHRSTIYRELNRNNESGVYLPIIAHQKALARAQNNRPNKLKNNGILR
DYVVRSLVNKGWSPEQISGRMKYQKLSFYVCPETIYRYVYQSNDKALYHSLAFKKPRRQKLFRRKHRTCRYGTPYLITQR
PEEIDSRERFGHWEGDTIEFKGNKSEVITTLVERKTRMVYLIKNTSKHSRGVMEKINKKFEKLPQKMFKTITFDQGSEFA
AHEYLEKNMKCEVYYCDVHSPWQKGSNENMNGRIRKYLPKNAKINLITQEELDLIAAKLNRCPRKCIGYKTPNELFIQQY
KNDCRTWS
>Mature_327_residues
GQYTQLSVTDRRRLFVFLDMGLSVREITHKLKRHRSTIYRELNRNNESGVYLPIIAHQKALARAQNNRPNKLKNNGILRD
YVVRSLVNKGWSPEQISGRMKYQKLSFYVCPETIYRYVYQSNDKALYHSLAFKKPRRQKLFRRKHRTCRYGTPYLITQRP
EEIDSRERFGHWEGDTIEFKGNKSEVITTLVERKTRMVYLIKNTSKHSRGVMEKINKKFEKLPQKMFKTITFDQGSEFAA
HEYLEKNMKCEVYYCDVHSPWQKGSNENMNGRIRKYLPKNAKINLITQEELDLIAAKLNRCPRKCIGYKTPNELFIQQYK
NDCRTWS

Specific function: Required for the transposition of the insertion element (Potential) [H]

COG id: COG2826

COG function: function code L; Transposase and inactivated derivatives, IS30 family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Escherichia coli, GI1786450, Length=325, Percent_Identity=38.4615384615385, Blast_Score=205, Evalue=4e-54,
Organism=Escherichia coli, GI1790736, Length=325, Percent_Identity=38.4615384615385, Blast_Score=204, Evalue=6e-54,
Organism=Escherichia coli, GI1787671, Length=325, Percent_Identity=38.4615384615385, Blast_Score=204, Evalue=6e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337
- InterPro:   IPR001598 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 39142; Mature: 39011

Theoretical pI: Translated: 10.49; Mature: 10.49

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGQYTQLSVTDRRRLFVFLDMGLSVREITHKLKRHRSTIYRELNRNNESGVYLPIIAHQK
CCCCEEEEECCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHHH
ALARAQNNRPNKLKNNGILRDYVVRSLVNKGWSPEQISGRMKYQKLSFYVCPETIYRYVY
HHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHCCCEEEEEEEEECHHHHHHHHH
QSNDKALYHSLAFKKPRRQKLFRRKHRTCRYGTPYLITQRPEEIDSRERFGHWEGDTIEF
HCCCHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHCCCCCCCEEEE
KGNKSEVITTLVERKTRMVYLIKNTSKHSRGVMEKINKKFEKLPQKMFKTITFDQGSEFA
CCCHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
AHEYLEKNMKCEVYYCDVHSPWQKGSNENMNGRIRKYLPKNAKINLITQEELDLIAAKLN
HHHHHHCCCCEEEEEECCCCHHHCCCCCCCCCHHHHHCCCCCEEEEEEHHHHHHHHHHHH
RCPRKCIGYKTPNELFIQQYKNDCRTWS
HCHHHHCCCCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
GQYTQLSVTDRRRLFVFLDMGLSVREITHKLKRHRSTIYRELNRNNESGVYLPIIAHQK
CCCEEEEECCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHHH
ALARAQNNRPNKLKNNGILRDYVVRSLVNKGWSPEQISGRMKYQKLSFYVCPETIYRYVY
HHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHCCCEEEEEEEEECHHHHHHHHH
QSNDKALYHSLAFKKPRRQKLFRRKHRTCRYGTPYLITQRPEEIDSRERFGHWEGDTIEF
HCCCHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHCCCCCCCEEEE
KGNKSEVITTLVERKTRMVYLIKNTSKHSRGVMEKINKKFEKLPQKMFKTITFDQGSEFA
CCCHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
AHEYLEKNMKCEVYYCDVHSPWQKGSNENMNGRIRKYLPKNAKINLITQEELDLIAAKLN
HHHHHHCCCCEEEEEECCCCHHHCCCCCCCCCHHHHHCCCCCEEEEEEHHHHHHHHHHHH
RCPRKCIGYKTPNELFIQQYKNDCRTWS
HCHHHHCCCCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]