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The map label for this gene is mycC [H]

Identifier: 148359503

GI number: 148359503

Start: 2275036

End: 2276559

Strand: Reverse

Name: mycC [H]

Synonym: LPC_1413

Alternate gene names: 148359503

Gene position: 2276559-2275036 (Counterclockwise)

Preceding gene: 148359504

Following gene: 148359502

Centisome position: 63.65

GC content: 39.3

Gene sequence:

>1524_bases
ATGGGATCCCCTGATCAGTACACGACAATTTGCACCTTATTTGAGGAGAAGGTAAAGCGATACCCGGAAAATACAGCAGT
TTATCTGGAAGATCGCCAAATTTCGTACGATGACTTTAATAAACAAGCCAATCAATACGCGCGGTATCTGCAAAGAAAAG
GGTTGCCATTAAATGGAATTGTCGGTGTTCAACTCGCGCGCTCTTTTGAAATGCTGGTGGCAATTTTTGCAGTATTAAAG
GCAGGTGGTGCCTATTTGCCGATAGATCCCATGGCCCCTGTTCTGAGAAATCAAGCCATCCTTGAAGACAGCCAAATCAA
ATTTCTAATTGTCAGTGATCATTCATTATTGACGGCAACCTCGGGTTTGCAAGTGTTAAGCATTCATGCCAATGTGAGCG
ATGAAGATTGCCATAATTTAAACCTGCCCAGGAAAAAACAGGATTTAGCGTATGTGATGTATACTTCAGGCACTACTGGC
AAGCCGAAAGGAGTCCTGATTTCACATCAAGCTTTGCTTAATCGTATTTTATGGATGCAAAACACATTTCCCTTGCAAAG
TCATGATGTAGTATTTCATAAAACACACATTTGTTTTGACATCTCTGTCTGGGAGACCTGTTGGTGGTCTATTGTGGGCG
CCGGAGTAGTACTTTTACCGCCAAGAAAAGAACATGACATCAAGCTGTTTATTCAAATGATTGAGCAATACCAAATCAAT
GTCATTCATTTTGTCCCCTCCGTATTACGAATATTTTTAAGTTACATCGAACAGGATTTTTCGATAAATCGCTTAAGCTC
TTTAAAGTACGTATTTTCAAGTGGAGAGGCGCTGGATGCCAAAAGTGTTAATCTATTCAACCAGTTATTTCAAGATCAAA
AAACAATATTAGTCAATTTATACGGGCCCACAGAGGCCACAATAGATGTTTCCTGTTTTATTTGTGAAAAGCACAAACGT
TTTGACACTGTACCCATTGGAAAGCCTATCCAAAATACTCAATTTTTTGTGTTAGACGAGCAGTTAAATTCAACCGGCTT
CGAACCTGGAGAGCTTTATATTTCCGGAGTTGGATTAGCTCAGGGTTATTTAAATAATCCAGTGCTGACTCAGGCATCGT
TTATAGACAATCCTTACTTGCCTGGAGAAAAAATGTATCGTACTGGAGATATAGTCCGTTGGAATCAAGAGGGTGAGTTA
TTATTTTTAGGCCGCAAGGACGATCAGGTCAAATTACGTGGCATACGTATTGAATTAGGAGAAATACAGCATCATTTATT
AGCGCATCCCAACATCCATGATGCCGTCGTGGTCTGTGAGAAAGTAGATTGTCTTGATCATAGAATGGTTGTATTCCTGA
TTGCTAATAACAAACAAACAAAGATTGGCAGCGCAGAACTGAAATCGTTTTTAAAAAACAGGCTCCCGGATTATATGATA
CCAGAGCATTATCTATGGTTAAGTGCATTTCCAATAAAAGAAAATGGAAAAATAGACAAAGAGAAGCTATTGAGTTTCAT
TTAG

Upstream 100 bases:

>100_bases
AGAGATGCCAAGGTAATGGAGATCATTGAAGGTACCTCACAAATGCACGAGATACTCATTTCAAAGCTTTGCAACAATAA
ATAGAGGATATTATTAAACA

Downstream 100 bases:

>100_bases
CATGAGCAATGCCCGAGAAATAAAAATAATCAGGGAAAATGACTTATACTTAATTAAAAGCCGGTTGCAGAAATAATATA
AACTATGTTTTATGTTATGA

Product: peptide synthetase

Products: pyrophosphate; AMP; enterobactin; pyrophosphate; L-Seryl-AMP [C]

Alternate protein names: ATP-dependent serine adenylase; SerA; Serine activase; ATP-dependent asparagine adenylase 3; AsnA 3; Asparagine activase 3 [H]

Number of amino acids: Translated: 507; Mature: 506

Protein sequence:

>507_residues
MGSPDQYTTICTLFEEKVKRYPENTAVYLEDRQISYDDFNKQANQYARYLQRKGLPLNGIVGVQLARSFEMLVAIFAVLK
AGGAYLPIDPMAPVLRNQAILEDSQIKFLIVSDHSLLTATSGLQVLSIHANVSDEDCHNLNLPRKKQDLAYVMYTSGTTG
KPKGVLISHQALLNRILWMQNTFPLQSHDVVFHKTHICFDISVWETCWWSIVGAGVVLLPPRKEHDIKLFIQMIEQYQIN
VIHFVPSVLRIFLSYIEQDFSINRLSSLKYVFSSGEALDAKSVNLFNQLFQDQKTILVNLYGPTEATIDVSCFICEKHKR
FDTVPIGKPIQNTQFFVLDEQLNSTGFEPGELYISGVGLAQGYLNNPVLTQASFIDNPYLPGEKMYRTGDIVRWNQEGEL
LFLGRKDDQVKLRGIRIELGEIQHHLLAHPNIHDAVVVCEKVDCLDHRMVVFLIANNKQTKIGSAELKSFLKNRLPDYMI
PEHYLWLSAFPIKENGKIDKEKLLSFI

Sequences:

>Translated_507_residues
MGSPDQYTTICTLFEEKVKRYPENTAVYLEDRQISYDDFNKQANQYARYLQRKGLPLNGIVGVQLARSFEMLVAIFAVLK
AGGAYLPIDPMAPVLRNQAILEDSQIKFLIVSDHSLLTATSGLQVLSIHANVSDEDCHNLNLPRKKQDLAYVMYTSGTTG
KPKGVLISHQALLNRILWMQNTFPLQSHDVVFHKTHICFDISVWETCWWSIVGAGVVLLPPRKEHDIKLFIQMIEQYQIN
VIHFVPSVLRIFLSYIEQDFSINRLSSLKYVFSSGEALDAKSVNLFNQLFQDQKTILVNLYGPTEATIDVSCFICEKHKR
FDTVPIGKPIQNTQFFVLDEQLNSTGFEPGELYISGVGLAQGYLNNPVLTQASFIDNPYLPGEKMYRTGDIVRWNQEGEL
LFLGRKDDQVKLRGIRIELGEIQHHLLAHPNIHDAVVVCEKVDCLDHRMVVFLIANNKQTKIGSAELKSFLKNRLPDYMI
PEHYLWLSAFPIKENGKIDKEKLLSFI
>Mature_506_residues
GSPDQYTTICTLFEEKVKRYPENTAVYLEDRQISYDDFNKQANQYARYLQRKGLPLNGIVGVQLARSFEMLVAIFAVLKA
GGAYLPIDPMAPVLRNQAILEDSQIKFLIVSDHSLLTATSGLQVLSIHANVSDEDCHNLNLPRKKQDLAYVMYTSGTTGK
PKGVLISHQALLNRILWMQNTFPLQSHDVVFHKTHICFDISVWETCWWSIVGAGVVLLPPRKEHDIKLFIQMIEQYQINV
IHFVPSVLRIFLSYIEQDFSINRLSSLKYVFSSGEALDAKSVNLFNQLFQDQKTILVNLYGPTEATIDVSCFICEKHKRF
DTVPIGKPIQNTQFFVLDEQLNSTGFEPGELYISGVGLAQGYLNNPVLTQASFIDNPYLPGEKMYRTGDIVRWNQEGELL
FLGRKDDQVKLRGIRIELGEIQHHLLAHPNIHDAVVVCEKVDCLDHRMVVFLIANNKQTKIGSAELKSFLKNRLPDYMIP
EHYLWLSAFPIKENGKIDKEKLLSFI

Specific function: This protein is a multifunctional enzyme, able to activate and polymerize the amino acids Ser and Asn as part of the synthesis of mycosubtilin. The Ser residue is further epimerized to the D-isomer form. The activation sites for these amino acids consist

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 acyl carrier domains [H]

Homologues:

Organism=Homo sapiens, GI187761345, Length=563, Percent_Identity=23.2682060390764, Blast_Score=109, Evalue=7e-24,
Organism=Homo sapiens, GI187761343, Length=563, Percent_Identity=23.2682060390764, Blast_Score=109, Evalue=7e-24,
Organism=Homo sapiens, GI45580730, Length=365, Percent_Identity=26.8493150684932, Blast_Score=108, Evalue=9e-24,
Organism=Homo sapiens, GI42544132, Length=549, Percent_Identity=21.6757741347905, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI156151445, Length=363, Percent_Identity=23.9669421487603, Blast_Score=90, Evalue=6e-18,
Organism=Homo sapiens, GI38505220, Length=537, Percent_Identity=21.415270018622, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI157311624, Length=527, Percent_Identity=21.8216318785579, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI157311622, Length=527, Percent_Identity=21.8216318785579, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI58082049, Length=527, Percent_Identity=21.4421252371917, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI13375727, Length=320, Percent_Identity=21.25, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1786801, Length=509, Percent_Identity=36.9351669941061, Blast_Score=318, Evalue=5e-88,
Organism=Escherichia coli, GI1786810, Length=520, Percent_Identity=23.6538461538462, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1788107, Length=553, Percent_Identity=22.0614828209765, Blast_Score=105, Evalue=7e-24,
Organism=Escherichia coli, GI145693145, Length=534, Percent_Identity=23.9700374531835, Blast_Score=103, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17556356, Length=482, Percent_Identity=27.8008298755187, Blast_Score=126, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17550940, Length=493, Percent_Identity=23.9350912778905, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17560140, Length=350, Percent_Identity=24.2857142857143, Blast_Score=101, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI32564422, Length=361, Percent_Identity=27.1468144044321, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI32564420, Length=361, Percent_Identity=27.1468144044321, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI32563687, Length=481, Percent_Identity=20.3742203742204, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17557194, Length=505, Percent_Identity=21.980198019802, Blast_Score=85, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI71985884, Length=368, Percent_Identity=22.2826086956522, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17538037, Length=508, Percent_Identity=22.244094488189, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17559526, Length=398, Percent_Identity=23.6180904522613, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71994694, Length=387, Percent_Identity=22.4806201550388, Blast_Score=73, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI71994690, Length=387, Percent_Identity=22.4806201550388, Blast_Score=73, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI71994703, Length=387, Percent_Identity=22.4806201550388, Blast_Score=73, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6319591, Length=603, Percent_Identity=24.7097844112769, Blast_Score=168, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6319699, Length=363, Percent_Identity=26.9972451790634, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24648676, Length=527, Percent_Identity=31.4990512333966, Blast_Score=233, Evalue=3e-61,
Organism=Drosophila melanogaster, GI24582852, Length=512, Percent_Identity=24.4140625, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24656500, Length=530, Percent_Identity=21.8867924528302, Blast_Score=76, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24581924, Length=368, Percent_Identity=23.9130434782609, Blast_Score=75, Evalue=8e-14,
Organism=Drosophila melanogaster, GI21356947, Length=501, Percent_Identity=19.9600798403194, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010071
- InterPro:   IPR009081
- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR001242
- InterPro:   IPR010060
- InterPro:   IPR006163
- InterPro:   IPR006162
- InterPro:   IPR001031 [H]

Pfam domain/function: PF00501 AMP-binding; PF00668 Condensation; PF00550 PP-binding; PF00975 Thioesterase [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 57809; Mature: 57678

Theoretical pI: Translated: 6.83; Mature: 6.83

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSPDQYTTICTLFEEKVKRYPENTAVYLEDRQISYDDFNKQANQYARYLQRKGLPLNGI
CCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
VGVQLARSFEMLVAIFAVLKAGGAYLPIDPMAPVLRNQAILEDSQIKFLIVSDHSLLTAT
HHHHHHHHHHHHHHHHHHHHCCCCEECCCCCHHHHHCCHHCCCCCEEEEEEECCCEEEEC
SGLQVLSIHANVSDEDCHNLNLPRKKQDLAYVMYTSGTTGKPKGVLISHQALLNRILWMQ
CCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCEEEEHHHHHHHHHHHC
NTFPLQSHDVVFHKTHICFDISVWETCWWSIVGAGVVLLPPRKEHDIKLFIQMIEQYQIN
CCCCCCCCCEEEEEEEEEEEEHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHCCC
VIHFVPSVLRIFLSYIEQDFSINRLSSLKYVFSSGEALDAKSVNLFNQLFQDQKTILVNL
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCEEEEEE
YGPTEATIDVSCFICEKHKRFDTVPIGKPIQNTQFFVLDEQLNSTGFEPGELYISGVGLA
ECCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCEEEEEEEEHH
QGYLNNPVLTQASFIDNPYLPGEKMYRTGDIVRWNQEGELLFLGRKDDQVKLRGIRIELG
HHCCCCCCEEEHHHCCCCCCCCCCEEECCCEEEECCCCCEEEEECCCCCEEEEEEEEEHH
EIQHHLLAHPNIHDAVVVCEKVDCLDHRMVVFLIANNKQTKIGSAELKSFLKNRLPDYMI
HHHHHHHCCCCCCCEEEEEEHHCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHCCCCCCC
PEHYLWLSAFPIKENGKIDKEKLLSFI
CCCEEEEEEECCCCCCCCCHHHHHHCC
>Mature Secondary Structure 
GSPDQYTTICTLFEEKVKRYPENTAVYLEDRQISYDDFNKQANQYARYLQRKGLPLNGI
CCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
VGVQLARSFEMLVAIFAVLKAGGAYLPIDPMAPVLRNQAILEDSQIKFLIVSDHSLLTAT
HHHHHHHHHHHHHHHHHHHHCCCCEECCCCCHHHHHCCHHCCCCCEEEEEEECCCEEEEC
SGLQVLSIHANVSDEDCHNLNLPRKKQDLAYVMYTSGTTGKPKGVLISHQALLNRILWMQ
CCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCEEEEHHHHHHHHHHHC
NTFPLQSHDVVFHKTHICFDISVWETCWWSIVGAGVVLLPPRKEHDIKLFIQMIEQYQIN
CCCCCCCCCEEEEEEEEEEEEHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHCCC
VIHFVPSVLRIFLSYIEQDFSINRLSSLKYVFSSGEALDAKSVNLFNQLFQDQKTILVNL
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCEEEEEE
YGPTEATIDVSCFICEKHKRFDTVPIGKPIQNTQFFVLDEQLNSTGFEPGELYISGVGLA
ECCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCEEEEEEEEHH
QGYLNNPVLTQASFIDNPYLPGEKMYRTGDIVRWNQEGELLFLGRKDDQVKLRGIRIELG
HHCCCCCCEEEHHHCCCCCCCCCCEEECCCEEEECCCCCEEEEECCCCCEEEEEEEEEHH
EIQHHLLAHPNIHDAVVVCEKVDCLDHRMVVFLIANNKQTKIGSAELKSFLKNRLPDYMI
HHHHHHHCCCCCCCEEEEEEHHCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHCCCCCCC
PEHYLWLSAFPIKENGKIDKEKLLSFI
CCCEEEEEEECCCCCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Phosphopantetheine. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 6 ATP; L-serine; 2,3-dihydroxybenzoate [C]

Specific reaction: 6 ATP + 3 L-serine + 3 2,3-dihydroxybenzoate = 6 pyrophosphate + 6 AMP + enterobactin ATP + L-serine = pyrophosphate + L-Seryl-AMP 6 ATP + 3 L-serine + 3 2,3-dihydroxybenzoate = 6 pyrophosphate + 6 AMP + enterobactin ATP + L-serine = pyrophosphate + L-Ser

General reaction: Transferases; Acyltransferases; Transferring groups other than amino-acyl groups [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10557314 [H]