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The map label for this gene is pksJ [H]

Identifier: 148359502

GI number: 148359502

Start: 2273550

End: 2274866

Strand: Reverse

Name: pksJ [H]

Synonym: LPC_1412

Alternate gene names: 148359502

Gene position: 2274866-2273550 (Counterclockwise)

Preceding gene: 148359503

Following gene: 148359500

Centisome position: 63.61

GC content: 40.02

Gene sequence:

>1317_bases
ATGAGTGACAATAACTACTCAAACAAAATTGCTATTGTTGGTATGGCTGTAAAATTTCCTGGGGCTAATGATCTTGATGA
ATATTGGGATTTATTGAAGTTTGGTAAAGAAGCAGTCACTAAATTTTCCGAAGAACAATTGCATCGGTCAGGTATTTCAG
AGCAATTAATCGCTAATCCATATTACAAACCATACAGAGGGATATTAGATGACCTGGAGTCATTCGATACCGCTCCGTTT
GAAAATACCACCAAAAATTTTGAATTGTTGGGTGTGCAAGGCAAAGTGATGTCGCATTTAACTTATCGGGCTTTGAGCAC
CATGAGAAGTCAGGAGCATCATTCAAATAACTACATAAAAAATACGGCTGTTTATATTGGCGCCAATAATCAACCTGCCT
CCCATTTCCTGGGAGCCGCCTACTACCAGTCGATTGATACTCAAAAAGCGATAGAAAGGTATTATTCAAAAGTCATTGCA
CCTTATATTTCTTATCAATTTGGATTTCAGGGTAGTTCTATTGATTTATATACAGCTTGCTCGTCTTCTCTGACAGCCGT
GATCCAAAGCTGTAGAGAGTTAAACAGTCATCAATGTGATATGGCAATTGCAGGCGCGTGCTTAATCGATTTACCCCAGG
AAGTTGGATATCTTTATGAAGAGAATGGCTTGTTTTCCTCCGACGGACATTGCCATTCTTTTGATGTTTCAGCATGTGGC
ACCTTATATTCAAACGGTGCTGGATTGGTTGTTCTAAAGCGGCTTGAAGATGCCATTGCGGATAATGATCTAATCCATGC
TGTGATTATCGGATCCGCGATGAATAACGATGGTAATTCTTCAAAAGAAGGATTTATGGCACCAGGGATCCATGGCCAAT
ATACTTGCTTACAGTCGGCTTGGAAAAACGCGGGGATTGAGCCAGGCGACCTGGATTATATTGCAGCTCATGGTGCTTCA
ACCAAGCTGGGTGATGCAACAGAAATATATGCCTTAAAAAAAGCGTTCAAAGACGTTAAAAAGACACATTCTTGTCCAAT
AAGCTCAGTAATTAGTAATCTGGGGCATACTGGTATTGTGTCCGGTATGGCGTCAATCATTAAAACTGTGCTTATGCTCA
AAAATATGGCTGTAGTCCCTTCAATTAATTTTGAAACTCCTAATCCAGATTTTATGTTGGAAGACTCTCCTATATACATT
GCCACTCAATTTCAGGATTTCCCTCAAATTAAAAAACACTATTTGGCCGGTGTTTCCAGTTATGGCGCAGGGGGAAGTAA
CACACACCTTGTTTTGCGTAGCTATGATGAAAACTAG

Upstream 100 bases:

>100_bases
GAAATAATATAAACTATGTTTTATGTTATGAACCCGTCATACGTCGTTTAATTAGTGTTTATTAAATTCTTGGTGCGGCC
AAGAAATGGAGCCATAAATT

Downstream 100 bases:

>100_bases
GGTTCTGTTGATATTTCATTTTTATTCCTGATAAAAGTTGACGTACTGCGGTTTGTTCGTTAGAGACTCACATACAATTT
AAACAGCAAGGAGTATGTCA

Product: polyketide synthase, type I

Products: L-tryptophan [Cytoplasm]; L-tyrosine [Cytoplasm]; Proton [Cytoplasm]; L-phenylalanine [Cytoplasm] [C]

Alternate protein names: PKS [H]

Number of amino acids: Translated: 438; Mature: 437

Protein sequence:

>438_residues
MSDNNYSNKIAIVGMAVKFPGANDLDEYWDLLKFGKEAVTKFSEEQLHRSGISEQLIANPYYKPYRGILDDLESFDTAPF
ENTTKNFELLGVQGKVMSHLTYRALSTMRSQEHHSNNYIKNTAVYIGANNQPASHFLGAAYYQSIDTQKAIERYYSKVIA
PYISYQFGFQGSSIDLYTACSSSLTAVIQSCRELNSHQCDMAIAGACLIDLPQEVGYLYEENGLFSSDGHCHSFDVSACG
TLYSNGAGLVVLKRLEDAIADNDLIHAVIIGSAMNNDGNSSKEGFMAPGIHGQYTCLQSAWKNAGIEPGDLDYIAAHGAS
TKLGDATEIYALKKAFKDVKKTHSCPISSVISNLGHTGIVSGMASIIKTVLMLKNMAVVPSINFETPNPDFMLEDSPIYI
ATQFQDFPQIKKHYLAGVSSYGAGGSNTHLVLRSYDEN

Sequences:

>Translated_438_residues
MSDNNYSNKIAIVGMAVKFPGANDLDEYWDLLKFGKEAVTKFSEEQLHRSGISEQLIANPYYKPYRGILDDLESFDTAPF
ENTTKNFELLGVQGKVMSHLTYRALSTMRSQEHHSNNYIKNTAVYIGANNQPASHFLGAAYYQSIDTQKAIERYYSKVIA
PYISYQFGFQGSSIDLYTACSSSLTAVIQSCRELNSHQCDMAIAGACLIDLPQEVGYLYEENGLFSSDGHCHSFDVSACG
TLYSNGAGLVVLKRLEDAIADNDLIHAVIIGSAMNNDGNSSKEGFMAPGIHGQYTCLQSAWKNAGIEPGDLDYIAAHGAS
TKLGDATEIYALKKAFKDVKKTHSCPISSVISNLGHTGIVSGMASIIKTVLMLKNMAVVPSINFETPNPDFMLEDSPIYI
ATQFQDFPQIKKHYLAGVSSYGAGGSNTHLVLRSYDEN
>Mature_437_residues
SDNNYSNKIAIVGMAVKFPGANDLDEYWDLLKFGKEAVTKFSEEQLHRSGISEQLIANPYYKPYRGILDDLESFDTAPFE
NTTKNFELLGVQGKVMSHLTYRALSTMRSQEHHSNNYIKNTAVYIGANNQPASHFLGAAYYQSIDTQKAIERYYSKVIAP
YISYQFGFQGSSIDLYTACSSSLTAVIQSCRELNSHQCDMAIAGACLIDLPQEVGYLYEENGLFSSDGHCHSFDVSACGT
LYSNGAGLVVLKRLEDAIADNDLIHAVIIGSAMNNDGNSSKEGFMAPGIHGQYTCLQSAWKNAGIEPGDLDYIAAHGAST
KLGDATEIYALKKAFKDVKKTHSCPISSVISNLGHTGIVSGMASIIKTVLMLKNMAVVPSINFETPNPDFMLEDSPIYIA
TQFQDFPQIKKHYLAGVSSYGAGGSNTHLVLRSYDEN

Specific function: Involved in some intermediate steps for the synthesis of the antibiotic polyketide bacillaene which is involved in secondary metabolism [H]

COG id: COG3321

COG function: function code Q; Polyketide synthase modules and related proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 5 acyl carrier domains [H]

Homologues:

Organism=Homo sapiens, GI41872631, Length=440, Percent_Identity=28.8636363636364, Blast_Score=168, Evalue=9e-42,
Organism=Homo sapiens, GI8923559, Length=289, Percent_Identity=26.643598615917, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI223972627, Length=145, Percent_Identity=31.0344827586207, Blast_Score=73, Evalue=5e-13,
Organism=Escherichia coli, GI1788663, Length=313, Percent_Identity=25.8785942492013, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1787337, Length=284, Percent_Identity=26.4084507042254, Blast_Score=86, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI212642053, Length=435, Percent_Identity=28.2758620689655, Blast_Score=159, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI17550940, Length=445, Percent_Identity=27.4157303370787, Blast_Score=152, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI71983541, Length=293, Percent_Identity=25.2559726962457, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI71983535, Length=266, Percent_Identity=26.3157894736842, Blast_Score=78, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6320904, Length=294, Percent_Identity=24.8299319727891, Blast_Score=77, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24581345, Length=430, Percent_Identity=26.9767441860465, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI221330659, Length=435, Percent_Identity=28.5057471264368, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI19920632, Length=435, Percent_Identity=28.5057471264368, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24644341, Length=462, Percent_Identity=24.2424242424242, Blast_Score=92, Evalue=1e-18,

Paralogues:

None

Copy number: 24024 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 30,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010071
- InterPro:   IPR009081
- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR000794
- InterPro:   IPR001242
- InterPro:   IPR018201
- InterPro:   IPR014031
- InterPro:   IPR014030
- InterPro:   IPR016040
- InterPro:   IPR006163
- InterPro:   IPR020842
- InterPro:   IPR020841
- InterPro:   IPR020807
- InterPro:   IPR013968
- InterPro:   IPR020806
- InterPro:   IPR006162
- InterPro:   IPR016039
- InterPro:   IPR016038 [H]

Pfam domain/function: PF00501 AMP-binding; PF00668 Condensation; PF00109 ketoacyl-synt; PF02801 Ketoacyl-synt_C; PF08659 KR; PF00550 PP-binding [H]

EC number: 2.3.1.41 [C]

Molecular weight: Translated: 47955; Mature: 47824

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: PS00606 B_KETOACYL_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDNNYSNKIAIVGMAVKFPGANDLDEYWDLLKFGKEAVTKFSEEQLHRSGISEQLIANP
CCCCCCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCC
YYKPYRGILDDLESFDTAPFENTTKNFELLGVQGKVMSHLTYRALSTMRSQEHHSNNYIK
CCCHHHHHHHHHHHCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCEE
NTAVYIGANNQPASHFLGAAYYQSIDTQKAIERYYSKVIAPYISYQFGFQGSSIDLYTAC
EEEEEEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHEECCCCCCEEEEHHH
SSSLTAVIQSCRELNSHQCDMAIAGACLIDLPQEVGYLYEENGLFSSDGHCHSFDVSACG
HHHHHHHHHHHHHHCCCCCCHHHHHHHHEECHHHHCCEEECCCCCCCCCCCEEECHHHHH
TLYSNGAGLVVLKRLEDAIADNDLIHAVIIGSAMNNDGNSSKEGFMAPGIHGQYTCLQSA
HHHCCCCCEEEHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHH
WKNAGIEPGDLDYIAAHGASTKLGDATEIYALKKAFKDVKKTHSCPISSVISNLGHTGIV
HHHCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHH
SGMASIIKTVLMLKNMAVVPSINFETPNPDFMLEDSPIYIATQFQDFPQIKKHYLAGVSS
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCEEEEECCHHHHHHHHHHHHHHHH
YGAGGSNTHLVLRSYDEN
CCCCCCCCEEEEEECCCC
>Mature Secondary Structure 
SDNNYSNKIAIVGMAVKFPGANDLDEYWDLLKFGKEAVTKFSEEQLHRSGISEQLIANP
CCCCCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCC
YYKPYRGILDDLESFDTAPFENTTKNFELLGVQGKVMSHLTYRALSTMRSQEHHSNNYIK
CCCHHHHHHHHHHHCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCEE
NTAVYIGANNQPASHFLGAAYYQSIDTQKAIERYYSKVIAPYISYQFGFQGSSIDLYTAC
EEEEEEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHEECCCCCCEEEEHHH
SSSLTAVIQSCRELNSHQCDMAIAGACLIDLPQEVGYLYEENGLFSSDGHCHSFDVSACG
HHHHHHHHHHHHHHCCCCCCHHHHHHHHEECHHHHCCEEECCCCCCCCCCCEEECHHHHH
TLYSNGAGLVVLKRLEDAIADNDLIHAVIIGSAMNNDGNSSKEGFMAPGIHGQYTCLQSA
HHHCCCCCEEEHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHH
WKNAGIEPGDLDYIAAHGASTKLGDATEIYALKKAFKDVKKTHSCPISSVISNLGHTGIV
HHHCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHH
SGMASIIKTVLMLKNMAVVPSINFETPNPDFMLEDSPIYIATQFQDFPQIKKHYLAGVSS
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCEEEEECCHHHHHHHHHHHHHHHH
YGAGGSNTHLVLRSYDEN
CCCCCCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: 2-Mercaptoethanol; DTT; EDTA [C]

Metal ions: Phosphate [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.04 {acetyl-ACP}} 0.031 {acetyl-ACP}} 0.138 {cis-9-hexadecenoyl-ACP}} 0.017 {cis-9-hexadecenoyl-ACP}} 0.012 {cis-3-decenoyl-ACP}} [C]

Substrates: L-tryptophan [Periplasm]; L-tyrosine [Periplasm]; Proton [Periplasm]; L-phenylalanine [Periplasm] [C]

Specific reaction: acyl-ACP + malonyl-ACP = beta-keto-enoyl-ACP + [acyl carrier protein] + CO2 acetyl-ACP + malonyl-ACP = [acyl carrier protein] + acetoacetyl-ACP + CO2 acyl-ACP + malonyl-ACP = [acyl carrier protein] + beta-ketoacyl-ACP + CO2 malonyl-ACP = acetyl-ACP + CO2

General reaction: Acyl group transfer [C]

Inhibitor: Acetyl-CoA; Acyl-CoA; Iodoacetamide; N-Ethyl maleimide acyl -ACP-derivatives protect, malonyl -ACP-decarboxyl ation, [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704258; 9384377; 10568751 [H]