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The map label for this gene is prfB [H]

Identifier: 148359322

GI number: 148359322

Start: 2067214

End: 2068221

Strand: Reverse

Name: prfB [H]

Synonym: LPC_1219

Alternate gene names: 148359322

Gene position: 2068221-2067214 (Counterclockwise)

Preceding gene: 148359323

Following gene: 148359321

Centisome position: 57.83

GC content: 42.36

Gene sequence:

>1008_bases
TTGGAAGAAGTAATTCGCGAATTGGAATCATCCAATGTGTGGGACAATCCGGAACAGGCTCAGGCATTGGGACGTGAAAG
AACTCAGCTGGAAACTATAGTCCACTCATTAAGCCAATTAAGCCAATCAATTAGTGATCAGAGGGAACTGTTTGAATTGG
CTCGAGAAGAAGATGATGAGCAAACCATCAACGACATTGGTGAGGAATTAAAATCAATTGAGCAACAAGTCGCCAGTTTG
GAATTTAGAAGAATGTTTTCTGGCAAAATGGACCACTCCAATGCTTATCTTGATATTCAAGCAGGTTCTGGCGGCACTGA
AGCGCAAGATTGGGCTGAAATGTTATTACGAATGTATTTACGTTGGGGAGAACATCACGGATTTACCACCGAGTTGATAG
AATGCTCTCCTGGTGAAGTGGCAGGAATTAAAAGTGCGACTATTCATTTTACAGGTGAATATGCGTTTGGTTGGTTAAGG
ACTGAAACAGGTGTCCATCGTTTAGTGAGAAAGTCGCCATTTGACTCTGGCAACAGGAGACACACTTCGTTTGCAGCTGT
TTTCGTCTCTCCTGAAATTGACGATGATATCGAAATTGAAATTAATCCAGCTGATTTGCGGATTGATACTTACAGAGCTT
CCGGGGCTGGTGGGCAGCATGTTAACCGTACTGATTCTGCCGTAAGAATTACACATATCCCTAGTGGGATAGTCGTTCAG
TGCCAGAATGATCGAAGCCAGCATAAAAATAAAGACCAGGCAATGAAGCAATTGAGGGCAAAGCTTTATGAGTTGGAAAT
GCAAAAGAAAAATGCGGAACAGCAAGCATTGGAAGCTAGCAAATCTGACATTGGTTGGGGATCACAAATTCGTTCTTACG
TATTAGACCAATCTCGTATAAAAGATTTGCGCACCGGTGTTGAAACCAGTAATACTCAGGCAGTATTGGATGGTTCATTA
GATCAATTTATTGAAGCCAGTTTAAAGGCAGGAGTAGGGCAAGCATGA

Upstream 100 bases:

>100_bases
ATGCTGGAAGTGAATCAAATTAATTTAAGTTTAATCGATCTTAATGAGCGCATAGAATCGCTTAGGGGGTATCTTTGACT
TCGAAGGTAAAAGTGAGCGC

Downstream 100 bases:

>100_bases
GTGAAGAACATGTACATTTAGATGAAAGTGAAGTTTATCATATTCGCAAGCAAAAATTAGCGGAATTGCGCACAGGTGGG
TTTAATTTTCCAAACACTTT

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MEEVIRELESSNVWDNPEQAQALGRERTQLETIVHSLSQLSQSISDQRELFELAREEDDEQTINDIGEELKSIEQQVASL
EFRRMFSGKMDHSNAYLDIQAGSGGTEAQDWAEMLLRMYLRWGEHHGFTTELIECSPGEVAGIKSATIHFTGEYAFGWLR
TETGVHRLVRKSPFDSGNRRHTSFAAVFVSPEIDDDIEIEINPADLRIDTYRASGAGGQHVNRTDSAVRITHIPSGIVVQ
CQNDRSQHKNKDQAMKQLRAKLYELEMQKKNAEQQALEASKSDIGWGSQIRSYVLDQSRIKDLRTGVETSNTQAVLDGSL
DQFIEASLKAGVGQA

Sequences:

>Translated_335_residues
MEEVIRELESSNVWDNPEQAQALGRERTQLETIVHSLSQLSQSISDQRELFELAREEDDEQTINDIGEELKSIEQQVASL
EFRRMFSGKMDHSNAYLDIQAGSGGTEAQDWAEMLLRMYLRWGEHHGFTTELIECSPGEVAGIKSATIHFTGEYAFGWLR
TETGVHRLVRKSPFDSGNRRHTSFAAVFVSPEIDDDIEIEINPADLRIDTYRASGAGGQHVNRTDSAVRITHIPSGIVVQ
CQNDRSQHKNKDQAMKQLRAKLYELEMQKKNAEQQALEASKSDIGWGSQIRSYVLDQSRIKDLRTGVETSNTQAVLDGSL
DQFIEASLKAGVGQA
>Mature_335_residues
MEEVIRELESSNVWDNPEQAQALGRERTQLETIVHSLSQLSQSISDQRELFELAREEDDEQTINDIGEELKSIEQQVASL
EFRRMFSGKMDHSNAYLDIQAGSGGTEAQDWAEMLLRMYLRWGEHHGFTTELIECSPGEVAGIKSATIHFTGEYAFGWLR
TETGVHRLVRKSPFDSGNRRHTSFAAVFVSPEIDDDIEIEINPADLRIDTYRASGAGGQHVNRTDSAVRITHIPSGIVVQ
CQNDRSQHKNKDQAMKQLRAKLYELEMQKKNAEQQALEASKSDIGWGSQIRSYVLDQSRIKDLRTGVETSNTQAVLDGSL
DQFIEASLKAGVGQA

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI34577120, Length=334, Percent_Identity=33.2335329341317, Blast_Score=175, Evalue=5e-44,
Organism=Homo sapiens, GI166795303, Length=306, Percent_Identity=34.640522875817, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI166795305, Length=209, Percent_Identity=33.9712918660287, Blast_Score=114, Evalue=2e-25,
Organism=Escherichia coli, GI2367172, Length=332, Percent_Identity=75, Blast_Score=526, Evalue=1e-150,
Organism=Escherichia coli, GI1787462, Length=243, Percent_Identity=43.6213991769547, Blast_Score=196, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI17542784, Length=298, Percent_Identity=30.8724832214765, Blast_Score=114, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6321295, Length=240, Percent_Identity=40.8333333333333, Blast_Score=153, Evalue=3e-38,
Organism=Drosophila melanogaster, GI19921226, Length=303, Percent_Identity=36.3036303630363, Blast_Score=171, Evalue=5e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 37654; Mature: 37654

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEVIRELESSNVWDNPEQAQALGRERTQLETIVHSLSQLSQSISDQRELFELAREEDDE
CHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
QTINDIGEELKSIEQQVASLEFRRMFSGKMDHSNAYLDIQAGSGGTEAQDWAEMLLRMYL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHH
RWGEHHGFTTELIECSPGEVAGIKSATIHFTGEYAFGWLRTETGVHRLVRKSPFDSGNRR
HHHCCCCCEEHEEECCCCCCCCCCEEEEEEECCHHEEEEHHHHHHHHHHHCCCCCCCCCC
HTSFAAVFVSPEIDDDIEIEINPADLRIDTYRASGAGGQHVNRTDSAVRITHIPSGIVVQ
CCEEEEEEECCCCCCCEEEEECCCCEEEEEEECCCCCCCCCCCCCCEEEEEECCCCEEEE
CQNDRSQHKNKDQAMKQLRAKLYELEMQKKNAEQQALEASKSDIGWGSQIRSYVLDQSRI
ECCCHHHHCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
KDLRTGVETSNTQAVLDGSLDQFIEASLKAGVGQA
HHHHHCCCCCCCCEEECCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MEEVIRELESSNVWDNPEQAQALGRERTQLETIVHSLSQLSQSISDQRELFELAREEDDE
CHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
QTINDIGEELKSIEQQVASLEFRRMFSGKMDHSNAYLDIQAGSGGTEAQDWAEMLLRMYL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHH
RWGEHHGFTTELIECSPGEVAGIKSATIHFTGEYAFGWLRTETGVHRLVRKSPFDSGNRR
HHHCCCCCEEHEEECCCCCCCCCCEEEEEEECCHHEEEEHHHHHHHHHHHCCCCCCCCCC
HTSFAAVFVSPEIDDDIEIEINPADLRIDTYRASGAGGQHVNRTDSAVRITHIPSGIVVQ
CCEEEEEEECCCCCCCEEEEECCCCEEEEEEECCCCCCCCCCCCCCEEEEEECCCCEEEE
CQNDRSQHKNKDQAMKQLRAKLYELEMQKKNAEQQALEASKSDIGWGSQIRSYVLDQSRI
ECCCHHHHCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
KDLRTGVETSNTQAVLDGSLDQFIEASLKAGVGQA
HHHHHCCCCCCCCEEECCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA