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The map label for this gene is flrB [H]

Identifier: 148359307

GI number: 148359307

Start: 2048961

End: 2049983

Strand: Reverse

Name: flrB [H]

Synonym: LPC_1204

Alternate gene names: 148359307

Gene position: 2049983-2048961 (Counterclockwise)

Preceding gene: 148359308

Following gene: 148359306

Centisome position: 57.32

GC content: 40.37

Gene sequence:

>1023_bases
ATGCATTATTCAAATTATCAAACTTGTGCAGTCAAACCACAGATAGAATTATCTGAATGGGACATTTTGTCCAGTTTGCC
TACCGCCTTGGTAATTTTGAATGCCCAAGGCAAAGTGGTGTGGTTAAATCCTTCAGCTGAAGCGATGCTTGGTTATGGAC
TGATTGGTTCACTATGGGTAGATGTGATTCAAAAAGCATTTGTTCCAAGAGCTGATGATGGGCATGAAGTGTCTCTGGCT
GATGGAAGGCGTGTGCACGTAGCCCTTTCGTCTTTAGAGAGTTTACCCGGTATATTGATTACTTTGTCTGACATTACTGC
AAGCAGGGATTATGAAGAAGCCAAAGAAAATGAAAAACGATTGGTTTCTATTGGTACAATGACCGCACAATTAGCTCATC
AGATTAGAACTCCTTTGTCTTCTGCCATATTGTATACCGAGCATTTAAATAATCTCCCAAGTTTGGATCATCGCATTAAA
AACTGGATTCACAGATTGCAAGAATGTCATGCCAGTATAGAGCAACAAATTCAGGATTTATTATTATTTGCCAGAGGAAC
CTCCATAGAGCCAAAGTATATCGATATGGAGTTTTGGTGCTCTCAATTAATGCAAAGAGCTCAGCCATACATCGAGTCTT
ATGCAGCTGTATTACATGTTAATAACCATCTTATTGCTCGTGAATCCTCCATACATGGAGAGTCTTTGATTGGAGCAATT
TTAAATCTGATAATCAATGCGCTGCAATCTGAGGCAACCCAAATTGATTTAACACTTGCATCCATGGATGATTCAGGTAT
ACAAATATCAGTAGAGGATAATGGAAACGGAATGTCTGAAGAAGTCAGGGCACAAGCATTTTCGCCGTTCTATACAACAA
AAGCACAAGGTACCGGCCTTGGATTAGCAGTGGTTTTTGCTGTAGTAAAAGCGCATGGTGGTCGAGTGACTCTTGAGTCG
ACTGAAGGGATAGGGACACAGGTAACTATTTATTTACCTTGCCTGAACAGAAACGTAGGGTAA

Upstream 100 bases:

>100_bases
ATCTGGTTTTCAATTGGTTAATTAATTGATTTCAAATACATTTTAATTTTGGCCTGAACTTTGCTAGAGACATTTCATAA
TAATAATTAAAATGAATAAC

Downstream 100 bases:

>100_bases
CTTTATCAAACAAAGGATTTTCTATGAGTCATGTTTTAATAGTTGAAGATGATTCAGTATTAAGAGAAGCGTTGGCTGAG
ACTATGACGATCTCAGGCCA

Product: sensor kinase HydH

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MHYSNYQTCAVKPQIELSEWDILSSLPTALVILNAQGKVVWLNPSAEAMLGYGLIGSLWVDVIQKAFVPRADDGHEVSLA
DGRRVHVALSSLESLPGILITLSDITASRDYEEAKENEKRLVSIGTMTAQLAHQIRTPLSSAILYTEHLNNLPSLDHRIK
NWIHRLQECHASIEQQIQDLLLFARGTSIEPKYIDMEFWCSQLMQRAQPYIESYAAVLHVNNHLIARESSIHGESLIGAI
LNLIINALQSEATQIDLTLASMDDSGIQISVEDNGNGMSEEVRAQAFSPFYTTKAQGTGLGLAVVFAVVKAHGGRVTLES
TEGIGTQVTIYLPCLNRNVG

Sequences:

>Translated_340_residues
MHYSNYQTCAVKPQIELSEWDILSSLPTALVILNAQGKVVWLNPSAEAMLGYGLIGSLWVDVIQKAFVPRADDGHEVSLA
DGRRVHVALSSLESLPGILITLSDITASRDYEEAKENEKRLVSIGTMTAQLAHQIRTPLSSAILYTEHLNNLPSLDHRIK
NWIHRLQECHASIEQQIQDLLLFARGTSIEPKYIDMEFWCSQLMQRAQPYIESYAAVLHVNNHLIARESSIHGESLIGAI
LNLIINALQSEATQIDLTLASMDDSGIQISVEDNGNGMSEEVRAQAFSPFYTTKAQGTGLGLAVVFAVVKAHGGRVTLES
TEGIGTQVTIYLPCLNRNVG
>Mature_340_residues
MHYSNYQTCAVKPQIELSEWDILSSLPTALVILNAQGKVVWLNPSAEAMLGYGLIGSLWVDVIQKAFVPRADDGHEVSLA
DGRRVHVALSSLESLPGILITLSDITASRDYEEAKENEKRLVSIGTMTAQLAHQIRTPLSSAILYTEHLNNLPSLDHRIK
NWIHRLQECHASIEQQIQDLLLFARGTSIEPKYIDMEFWCSQLMQRAQPYIESYAAVLHVNNHLIARESSIHGESLIGAI
LNLIINALQSEATQIDLTLASMDDSGIQISVEDNGNGMSEEVRAQAFSPFYTTKAQGTGLGLAVVFAVVKAHGGRVTLES
TEGIGTQVTIYLPCLNRNVG

Specific function: Member of the two-component regulatory system AtoS/AtoC; may activate AtoC by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=339, Percent_Identity=28.9085545722714, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1790436, Length=225, Percent_Identity=30.2222222222222, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1790346, Length=230, Percent_Identity=28.695652173913, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1786600, Length=331, Percent_Identity=24.773413897281, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1788713, Length=355, Percent_Identity=24.2253521126761, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1789808, Length=213, Percent_Identity=26.7605633802817, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1786783, Length=221, Percent_Identity=24.8868778280543, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 37385; Mature: 37385

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS50112 PAS ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHYSNYQTCAVKPQIELSEWDILSSLPTALVILNAQGKVVWLNPSAEAMLGYGLIGSLWV
CCCCCCCEEEECCCCCCHHHHHHHHCCCEEEEEECCCEEEEECCCHHHHHHHHHHHHHHH
DVIQKAFVPRADDGHEVSLADGRRVHVALSSLESLPGILITLSDITASRDYEEAKENEKR
HHHHHHCCCCCCCCCEEEECCCCEEEHHHHHHHHCCCEEEEEECHHCCCCHHHHHCCHHH
LVSIGTMTAQLAHQIRTPLSSAILYTEHLNNLPSLDHRIKNWIHRLQECHASIEQQIQDL
EEEHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLFARGTSIEPKYIDMEFWCSQLMQRAQPYIESYAAVLHVNNHLIARESSIHGESLIGAI
HHHHCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHEEECCEEEEECCCCCHHHHHHHH
LNLIINALQSEATQIDLTLASMDDSGIQISVEDNGNGMSEEVRAQAFSPFYTTKAQGTGL
HHHHHHHHHCCCEEEEEEEEECCCCCEEEEEECCCCCCHHHHHHHHCCCCEECCCCCCCH
GLAVVFAVVKAHGGRVTLESTEGIGTQVTIYLPCLNRNVG
HHHHHHHHHHCCCCEEEEECCCCCCEEEEEEEEECCCCCC
>Mature Secondary Structure
MHYSNYQTCAVKPQIELSEWDILSSLPTALVILNAQGKVVWLNPSAEAMLGYGLIGSLWV
CCCCCCCEEEECCCCCCHHHHHHHHCCCEEEEEECCCEEEEECCCHHHHHHHHHHHHHHH
DVIQKAFVPRADDGHEVSLADGRRVHVALSSLESLPGILITLSDITASRDYEEAKENEKR
HHHHHHCCCCCCCCCEEEECCCCEEEHHHHHHHHCCCEEEEEECHHCCCCHHHHHCCHHH
LVSIGTMTAQLAHQIRTPLSSAILYTEHLNNLPSLDHRIKNWIHRLQECHASIEQQIQDL
EEEHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLFARGTSIEPKYIDMEFWCSQLMQRAQPYIESYAAVLHVNNHLIARESSIHGESLIGAI
HHHHCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHEEECCEEEEECCCCCHHHHHHHH
LNLIINALQSEATQIDLTLASMDDSGIQISVEDNGNGMSEEVRAQAFSPFYTTKAQGTGL
HHHHHHHHHCCCEEEEEEEEECCCCCEEEEEECCCCCCHHHHHHHHCCCCEECCCCCCCH
GLAVVFAVVKAHGGRVTLESTEGIGTQVTIYLPCLNRNVG
HHHHHHHHHHCCCCEEEEECCCCCCEEEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8346225; 9097040; 9278503 [H]