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The map label for this gene is rrf [H]

Identifier: 148359246

GI number: 148359246

Start: 1977564

End: 1978121

Strand: Reverse

Name: rrf [H]

Synonym: LPC_1140

Alternate gene names: 148359246

Gene position: 1978121-1977564 (Counterclockwise)

Preceding gene: 148359247

Following gene: 148359245

Centisome position: 55.31

GC content: 39.78

Gene sequence:

>558_bases
ATGATCAATGAGATTAAGCAAGATTCAGAAAAACGCATGAAAAAGACTATTGAGGCTTTGCATACGGATATGTCAAAAAT
CCGTACCGGCAGGGCGAATGCCAGTCTTTTAGATCATGTCATGGTTGATTATTATGGAAGTCCTACACCTTTAAGCCAGG
TTGCCAACATCACTACCAGTGACTCCCGAACTATTTTAGTTACACCCTGGGAAAAATCTATGGTCGCAGCTATTGAAAAG
GCTATACTAAACTCTGATTTGGGTTTGAATCCTGCAACTGCTGGAACAGCCATTCGAGTTCCGATGCCACCTTTAACAGA
GGAACGCAGAAAGGAATTGATTAAAGTTGTTCGCCATGAAGGGGAACAAGGAAGAGTTTCAATTCGAAATATCAGAAGGG
ATGCCAATAATCAGCTAAAGGAACTTGTTAAAGAAAAAGCAATTTCTGAAGACGATGAACGACGTGCAGCAGAAGCGATC
CAAAAGCTGACTGATAGATATATTTCTGAAGTTGATGCAGTATTAGCCGAGAAAGAAAAAGATTTAATGGAAATTTAA

Upstream 100 bases:

>100_bases
GATCAGGGAATGCCTTTACAGGTGTTTGATATGGCGGCTCCTAAAGCATTAAAACGAATTGTAACTGGAGAACGAGTAGG
AACTATAGTCGGAGCTAATC

Downstream 100 bases:

>100_bases
GGTATAGATGTTACTCCTTTTATATAATAGAGCTATAGTTCTTGTTTTTAGTACAAGAAAAAACATACTCTAATTATATA
ATGCTAAATTTTTGTCATTC

Product: ribosome recycling factor

Products: NA

Alternate protein names: RRF; Ribosome-releasing factor [H]

Number of amino acids: Translated: 185; Mature: 185

Protein sequence:

>185_residues
MINEIKQDSEKRMKKTIEALHTDMSKIRTGRANASLLDHVMVDYYGSPTPLSQVANITTSDSRTILVTPWEKSMVAAIEK
AILNSDLGLNPATAGTAIRVPMPPLTEERRKELIKVVRHEGEQGRVSIRNIRRDANNQLKELVKEKAISEDDERRAAEAI
QKLTDRYISEVDAVLAEKEKDLMEI

Sequences:

>Translated_185_residues
MINEIKQDSEKRMKKTIEALHTDMSKIRTGRANASLLDHVMVDYYGSPTPLSQVANITTSDSRTILVTPWEKSMVAAIEK
AILNSDLGLNPATAGTAIRVPMPPLTEERRKELIKVVRHEGEQGRVSIRNIRRDANNQLKELVKEKAISEDDERRAAEAI
QKLTDRYISEVDAVLAEKEKDLMEI
>Mature_185_residues
MINEIKQDSEKRMKKTIEALHTDMSKIRTGRANASLLDHVMVDYYGSPTPLSQVANITTSDSRTILVTPWEKSMVAAIEK
AILNSDLGLNPATAGTAIRVPMPPLTEERRKELIKVVRHEGEQGRVSIRNIRRDANNQLKELVKEKAISEDDERRAAEAI
QKLTDRYISEVDAVLAEKEKDLMEI

Specific function: Responsible for the release of ribosomes from messenger RNA at the termination of protein biosynthesis. May increase the efficiency of translation by recycling ribosomes from one round of translation to another [H]

COG id: COG0233

COG function: function code J; Ribosome recycling factor

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RRF family [H]

Homologues:

Organism=Homo sapiens, GI20270317, Length=173, Percent_Identity=33.5260115606936, Blast_Score=85, Evalue=4e-17,
Organism=Homo sapiens, GI291042689, Length=157, Percent_Identity=33.7579617834395, Blast_Score=75, Evalue=3e-14,
Organism=Escherichia coli, GI1786368, Length=185, Percent_Identity=57.8378378378378, Blast_Score=233, Evalue=4e-63,
Organism=Drosophila melanogaster, GI24661488, Length=173, Percent_Identity=28.3236994219653, Blast_Score=76, Evalue=1e-14,

Paralogues:

None

Copy number: 840 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002661
- InterPro:   IPR015998 [H]

Pfam domain/function: PF01765 RRF [H]

EC number: NA

Molecular weight: Translated: 20863; Mature: 20863

Theoretical pI: Translated: 6.82; Mature: 6.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINEIKQDSEKRMKKTIEALHTDMSKIRTGRANASLLDHVMVDYYGSPTPLSQVANITTS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHCCCC
DSRTILVTPWEKSMVAAIEKAILNSDLGLNPATAGTAIRVPMPPLTEERRKELIKVVRHE
CCCEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHC
GEQGRVSIRNIRRDANNQLKELVKEKAISEDDERRAAEAIQKLTDRYISEVDAVLAEKEK
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLMEI
HHHCC
>Mature Secondary Structure
MINEIKQDSEKRMKKTIEALHTDMSKIRTGRANASLLDHVMVDYYGSPTPLSQVANITTS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHCCCC
DSRTILVTPWEKSMVAAIEKAILNSDLGLNPATAGTAIRVPMPPLTEERRKELIKVVRHE
CCCEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHC
GEQGRVSIRNIRRDANNQLKELVKEKAISEDDERRAAEAIQKLTDRYISEVDAVLAEKEK
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLMEI
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA