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The map label for this gene is iolD [H]

Identifier: 148359186

GI number: 148359186

Start: 1892468

End: 1894339

Strand: Reverse

Name: iolD [H]

Synonym: LPC_1080

Alternate gene names: 148359186

Gene position: 1894339-1892468 (Counterclockwise)

Preceding gene: 148359187

Following gene: 148359185

Centisome position: 52.97

GC content: 40.76

Gene sequence:

>1872_bases
ATGAAGATTAAATTAACTATGGCTCAGGCCCTATTAAGATTTTTAGCTAATCAGTATGTCGTTCTGGATAATAATGAATA
CCGTTTCATCAATGGTGTATTTGGAATATTTGGCCATGGTAATGTAACCGGATTAGGTGAGGCATTGGAATACGATGCTC
AGGGTTTAATTTATTATCAGGGACATAATGAACAAGGAATGGCTCACGCAGCAACCGCCTATGCCAAACAAAAAAATCGG
CTAGGGATAATGGCTTGTACTTCATCAATTGGTCCTGGCGCTACTAATATGATTACTGCAGCGGCGACGGCGACAACCAA
TAGAATTCCATTACTATTATTGCCAGGTGATGTATTCAGTTGCAGGCAACCAGATCCGGTTCTCCAACAATTGGAGGTGC
CATATGACTACACCATATCTGTTAATGATTGCTTTAAACCAGTAAGTAAGTTTTGGGACAGGATAACCAGACCTGAACAG
TTAATGACAGCGTGTATGCATGCTATGAGGGTATTAACCGATCCAGTTGAAACAGGTACTGTTACTTTGTGTTTGCCTCA
AGACGTTCAGTCTGAAGCTTATGAATATGAGGAATCTTTTTTTAAAAAGCGCTTATGGCGTATTGAGAGAGAGAGGATAA
GTGATGCAATGTTGGCAGAGGCCATACGGCATATCAGAAAGGCTAAGCAACCATTAATCATTGCAGGTGGTGGTGTGCAT
TATTCACTGGCTTCGGAGACTTTATCAGAGTTTGCATTAAAACATCGCATTCCTGTTGCTGAAACCCAAGCAGGAAAAAG
TAGCCTGGCAGCGAAACACCCCATGAACGTTGGTGGAATAGGTGTTACTGGATCTGAAATAGCGAATTCTCTGGCAGCTC
AGGCTGACGTTATTTTGGTTATCGGGTCTCGATTACAGGATTTTACGACAGCTTCCAAATGGGCTTTTAAAAACCAGGAC
TGCCAAATCATTCATCTTAATGTATCACGTCTTGATGCCATGAAAATGAATGGAGTTATGTTAAAAGGAGATGCTAAAAC
TGGTTTAGAGCAATTGGGGAAAGAGCTGGCAGATTATCAAACCTCCCTCTCTTACCAGCAGTTAATACGTCATTATCAAA
ATGAATGGGCCCAAGAGTTAGATAAATTGACTCAATCTTATTCTACAGAGCAGGCTGGGTTACACCAAACTGCTGTTCTG
GGACTACTCAATCAGTATGTTACTGAAGAGGATGTGATAATTAGTGCAGCGGGGAGTTTGCCAGGTGATTTGCATCGAAT
TTGGCAATCCAAAAGGGCAAAGGATTATCATTTAGAGTACGCTTATTCCTGTATGGGATATGAGGTTGCAGCGGGTCTAG
GAGTACGTATTGCAAAAGAAAATCAGGAAGGAGAAGTCTACGTTTTAGTTGGGGATGGCAGTTTTATCATGATGCATTCA
GAGCTTTTAACCGCCATTCAGGAACGCAAGAAAATTACAATTCTCATTTTTGATAATCATGGATTTCAGTGCATTAGAAA
TTTACAGGAAGGGAATGGTAGTCTGGGTTTTGGTAATGAATTTCGTTATAGAGAGCCAACTACTAATGCTTTAAGTGGCG
ATTATTTGCCCATAGATTTTTGTAAATACGCAGAGGGATTGGGGGCAGCCACTTATTTTGTTAAATCCTATGAGCAATTC
CGGACTGCTTTAGACTCAGCAAAAAAACAGGAAAATAGTACGGTTATTGTTCTTCCTGTGTTACCAAAGACCATGAGTCA
AGGATATAAGACCTGGTGGCGGGTTGGAGTTGCTGAGGTATCTCAATCAGAATCAGTGAGTAAAGCTTACCAAATGATGC
AAGAGCAAATTGAAACAGTTAGACAATATTGA

Upstream 100 bases:

>100_bases
AAGACCATGAATGGACAAGGACAAGCAGGGCAAATTTAAGAGCATGGCAACAGAAACAGTAGTAATCCTTATCTGCAGTA
TGATTTTGAGAGTTAAATAA

Downstream 100 bases:

>100_bases
AGATGGAAGTGAAGATGAGTATTCAACTTGGTATCGCGCCAATTAATTGGTGCAATGATGATGATCCGAATTTAGGTAAA
GACATTAGTTTTGAGCAATG

Product: myo-inositol catabolism protein IolD

Products: pyruvate; 2-aceto-2-hydroxy-butyrate; CO2 [C]

Alternate protein names: THcHDO hydrolase 1 [H]

Number of amino acids: Translated: 623; Mature: 623

Protein sequence:

>623_residues
MKIKLTMAQALLRFLANQYVVLDNNEYRFINGVFGIFGHGNVTGLGEALEYDAQGLIYYQGHNEQGMAHAATAYAKQKNR
LGIMACTSSIGPGATNMITAAATATTNRIPLLLLPGDVFSCRQPDPVLQQLEVPYDYTISVNDCFKPVSKFWDRITRPEQ
LMTACMHAMRVLTDPVETGTVTLCLPQDVQSEAYEYEESFFKKRLWRIERERISDAMLAEAIRHIRKAKQPLIIAGGGVH
YSLASETLSEFALKHRIPVAETQAGKSSLAAKHPMNVGGIGVTGSEIANSLAAQADVILVIGSRLQDFTTASKWAFKNQD
CQIIHLNVSRLDAMKMNGVMLKGDAKTGLEQLGKELADYQTSLSYQQLIRHYQNEWAQELDKLTQSYSTEQAGLHQTAVL
GLLNQYVTEEDVIISAAGSLPGDLHRIWQSKRAKDYHLEYAYSCMGYEVAAGLGVRIAKENQEGEVYVLVGDGSFIMMHS
ELLTAIQERKKITILIFDNHGFQCIRNLQEGNGSLGFGNEFRYREPTTNALSGDYLPIDFCKYAEGLGAATYFVKSYEQF
RTALDSAKKQENSTVIVLPVLPKTMSQGYKTWWRVGVAEVSQSESVSKAYQMMQEQIETVRQY

Sequences:

>Translated_623_residues
MKIKLTMAQALLRFLANQYVVLDNNEYRFINGVFGIFGHGNVTGLGEALEYDAQGLIYYQGHNEQGMAHAATAYAKQKNR
LGIMACTSSIGPGATNMITAAATATTNRIPLLLLPGDVFSCRQPDPVLQQLEVPYDYTISVNDCFKPVSKFWDRITRPEQ
LMTACMHAMRVLTDPVETGTVTLCLPQDVQSEAYEYEESFFKKRLWRIERERISDAMLAEAIRHIRKAKQPLIIAGGGVH
YSLASETLSEFALKHRIPVAETQAGKSSLAAKHPMNVGGIGVTGSEIANSLAAQADVILVIGSRLQDFTTASKWAFKNQD
CQIIHLNVSRLDAMKMNGVMLKGDAKTGLEQLGKELADYQTSLSYQQLIRHYQNEWAQELDKLTQSYSTEQAGLHQTAVL
GLLNQYVTEEDVIISAAGSLPGDLHRIWQSKRAKDYHLEYAYSCMGYEVAAGLGVRIAKENQEGEVYVLVGDGSFIMMHS
ELLTAIQERKKITILIFDNHGFQCIRNLQEGNGSLGFGNEFRYREPTTNALSGDYLPIDFCKYAEGLGAATYFVKSYEQF
RTALDSAKKQENSTVIVLPVLPKTMSQGYKTWWRVGVAEVSQSESVSKAYQMMQEQIETVRQY
>Mature_623_residues
MKIKLTMAQALLRFLANQYVVLDNNEYRFINGVFGIFGHGNVTGLGEALEYDAQGLIYYQGHNEQGMAHAATAYAKQKNR
LGIMACTSSIGPGATNMITAAATATTNRIPLLLLPGDVFSCRQPDPVLQQLEVPYDYTISVNDCFKPVSKFWDRITRPEQ
LMTACMHAMRVLTDPVETGTVTLCLPQDVQSEAYEYEESFFKKRLWRIERERISDAMLAEAIRHIRKAKQPLIIAGGGVH
YSLASETLSEFALKHRIPVAETQAGKSSLAAKHPMNVGGIGVTGSEIANSLAAQADVILVIGSRLQDFTTASKWAFKNQD
CQIIHLNVSRLDAMKMNGVMLKGDAKTGLEQLGKELADYQTSLSYQQLIRHYQNEWAQELDKLTQSYSTEQAGLHQTAVL
GLLNQYVTEEDVIISAAGSLPGDLHRIWQSKRAKDYHLEYAYSCMGYEVAAGLGVRIAKENQEGEVYVLVGDGSFIMMHS
ELLTAIQERKKITILIFDNHGFQCIRNLQEGNGSLGFGNEFRYREPTTNALSGDYLPIDFCKYAEGLGAATYFVKSYEQF
RTALDSAKKQENSTVIVLPVLPKTMSQGYKTWWRVGVAEVSQSESVSKAYQMMQEQIETVRQY

Specific function: Involved in the cleavage of the C1-C2 bond of 3D- (3,5/4)-trihydroxycyclohexane-1,2-dione (THcHDO) to yield 5-deoxy- glucuronate (5DG) [H]

COG id: COG3962

COG function: function code E; Acetolactate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=563, Percent_Identity=23.0905861456483, Blast_Score=80, Evalue=5e-15,
Organism=Escherichia coli, GI1790104, Length=510, Percent_Identity=24.9019607843137, Blast_Score=135, Evalue=1e-32,
Organism=Escherichia coli, GI87081685, Length=563, Percent_Identity=23.9786856127886, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI1788716, Length=595, Percent_Identity=25.0420168067227, Blast_Score=103, Evalue=4e-23,
Organism=Escherichia coli, GI1786717, Length=523, Percent_Identity=21.6061185468451, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1787096, Length=491, Percent_Identity=20.162932790224, Blast_Score=70, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17531299, Length=511, Percent_Identity=23.679060665362, Blast_Score=80, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17531301, Length=514, Percent_Identity=23.7354085603113, Blast_Score=79, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6323755, Length=568, Percent_Identity=23.943661971831, Blast_Score=115, Evalue=1e-26,
Organism=Drosophila melanogaster, GI19922626, Length=603, Percent_Identity=22.2222222222222, Blast_Score=89, Evalue=8e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: 2.2.1.6 [C]

Molecular weight: Translated: 69475; Mature: 69475

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIKLTMAQALLRFLANQYVVLDNNEYRFINGVFGIFGHGNVTGLGEALEYDAQGLIYYQ
CEEEEHHHHHHHHHHCCCEEEEECCCEEEEEEEEEECCCCCCCCCCHHHHCCCCEEEEEE
GHNEQGMAHAATAYAKQKNRLGIMACTSSIGPGATNMITAAATATTNRIPLLLLPGDVFS
CCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCHHHEEEEHHCCCCCCEEEEECCCCCC
CRQPDPVLQQLEVPYDYTISVNDCFKPVSKFWDRITRPEQLMTACMHAMRVLTDPVETGT
CCCCCHHHHHHCCCCEEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCE
VTLCLPQDVQSEAYEYEESFFKKRLWRIERERISDAMLAEAIRHIRKAKQPLIIAGGGVH
EEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEE
YSLASETLSEFALKHRIPVAETQAGKSSLAAKHPMNVGGIGVTGSEIANSLAAQADVILV
EHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCEEEE
IGSRLQDFTTASKWAFKNQDCQIIHLNVSRLDAMKMNGVMLKGDAKTGLEQLGKELADYQ
ECCHHHHHHHHHHCEECCCCCEEEEEEHHHHHHHEECCEEEECCHHHHHHHHHHHHHHHH
TSLSYQQLIRHYQNEWAQELDKLTQSYSTEQAGLHQTAVLGLLNQYVTEEDVIISAAGSL
HCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHCCCCCEEEEECCCC
PGDLHRIWQSKRAKDYHLEYAYSCMGYEVAAGLGVRIAKENQEGEVYVLVGDGSFIMMHS
CHHHHHHHHHCCCCHHHHHHHHHHCCHHHHCCCCEEEEECCCCCEEEEEEECCCEEEEHH
ELLTAIQERKKITILIFDNHGFQCIRNLQEGNGSLGFGNEFRYREPTTNALSGDYLPIDF
HHHHHHHHCCEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
CKYAEGLGAATYFVKSYEQFRTALDSAKKQENSTVIVLPVLPKTMSQGYKTWWRVGVAEV
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHH
SQSESVSKAYQMMQEQIETVRQY
HHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKIKLTMAQALLRFLANQYVVLDNNEYRFINGVFGIFGHGNVTGLGEALEYDAQGLIYYQ
CEEEEHHHHHHHHHHCCCEEEEECCCEEEEEEEEEECCCCCCCCCCHHHHCCCCEEEEEE
GHNEQGMAHAATAYAKQKNRLGIMACTSSIGPGATNMITAAATATTNRIPLLLLPGDVFS
CCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCHHHEEEEHHCCCCCCEEEEECCCCCC
CRQPDPVLQQLEVPYDYTISVNDCFKPVSKFWDRITRPEQLMTACMHAMRVLTDPVETGT
CCCCCHHHHHHCCCCEEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCE
VTLCLPQDVQSEAYEYEESFFKKRLWRIERERISDAMLAEAIRHIRKAKQPLIIAGGGVH
EEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEE
YSLASETLSEFALKHRIPVAETQAGKSSLAAKHPMNVGGIGVTGSEIANSLAAQADVILV
EHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCEEEE
IGSRLQDFTTASKWAFKNQDCQIIHLNVSRLDAMKMNGVMLKGDAKTGLEQLGKELADYQ
ECCHHHHHHHHHHCEECCCCCEEEEEEHHHHHHHEECCEEEECCHHHHHHHHHHHHHHHH
TSLSYQQLIRHYQNEWAQELDKLTQSYSTEQAGLHQTAVLGLLNQYVTEEDVIISAAGSL
HCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHCCCCCEEEEECCCC
PGDLHRIWQSKRAKDYHLEYAYSCMGYEVAAGLGVRIAKENQEGEVYVLVGDGSFIMMHS
CHHHHHHHHHCCCCHHHHHHHHHHCCHHHHCCCCEEEEECCCCCEEEEEEECCCEEEEHH
ELLTAIQERKKITILIFDNHGFQCIRNLQEGNGSLGFGNEFRYREPTTNALSGDYLPIDF
HHHHHHHHCCEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
CKYAEGLGAATYFVKSYEQFRTALDSAKKQENSTVIVLPVLPKTMSQGYKTWWRVGVAEV
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHH
SQSESVSKAYQMMQEQIETVRQY
HHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 4002 [C]

Specific activity: 53

Km value (mM): 5 {2-oxobutanoate}} 1 {2-oxobutanoate}} 7.6 {pyruvate}} 7 {pyruvate}} 2.9 {pyruvate}} 2.6 {pyruvate}} 1.3 {pyruvate}} 1 {pyruvate}} [C]

Substrates: acetolactate; CO2; pyruvate; 2-oxobutanoate [C]

Specific reaction: acetolactate + CO2 = 2 pyruvate pyruvate + 2-oxobutanoate = 2-aceto-2-hydroxy-butyrate + CO2 [C]

General reaction: C-C bond formation; decarboxylation [C]

Inhibitor: @OXOB02.txt*2-oxobutanoate!; chlorimuronethyl; chlorsulphuron; Ile; imazapyr; Leu; Val [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA