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The map label for this gene is bla [H]

Identifier: 148359153

GI number: 148359153

Start: 1853872

End: 1854672

Strand: Reverse

Name: bla [H]

Synonym: LPC_1045

Alternate gene names: 148359153

Gene position: 1854672-1853872 (Counterclockwise)

Preceding gene: 148359154

Following gene: 148359151

Centisome position: 51.86

GC content: 37.83

Gene sequence:

>801_bases
ATGAAGAAAATAATTATATTTTTGTGCACAGGATTATTATTTTGCGTCTCTACTTGGGCTCAAGGCACCTGTTTTTTAGC
AAAAGAAAATCAAACTGTTTTAAAACGTGAGGGCAATGATTGTGATCAACGCTATTCGCCAGCATCAACTTTTAAAATTG
CCCTTAGCCTCATGGGCTTTGATTCTGGAATATTAAAGGATGAGTTGCATCCTGAATGGCCATACAAAAAAGAATATGAA
CTTTATCTGAATGTATGGAAGTACCCTCAAAACCCGCATACCTGGATCAGAGACTCCTGTGTTTGGTATTCACAGGCATT
AACTCGCCAATTGGGCATGAAACGGTTTAAAGGGTATGTCGACGCATTTCACTACGGTAATCAGGATGTTTCGGGAGACA
AAGGTCAAAATAACGGTTTAACCCATGCCTGGCTTTCAAGCTCACTTTCTATCTCACCCACAGAGCAAATTCAATTTCTA
CAAAAAATAATCTATAAGAAACTACCGGTGAGTCAAAAAGCTTATACCATGACTAAAAACATCATGTACATCCAGGAGTT
GCCTGGTGGGTGGAAGCTTTATGGAAAAACAGGAACTGGTCAACAGTTAACAAAAGATAAAAGTCAAAAATTGCCTTTAC
AACACGGATGGTTCGTAGGTTGGATTGAAAAGGATGAGCGTGTGATTACCTTTGCAAAACATATTGCAGACAGTAAAGAG
AATACCACTTTTGCCAGTTTTAGAGCGAAAAATGATACATTGATTCAATTGTTTAACCTGATTAATGAACTGGAGAAATA
A

Upstream 100 bases:

>100_bases
CCGGTATTCCGAAATGTCGCAATGGATGCTCTGATCAGTGTAAATCAATAACTAGCTTATGGGTTTGCATATCCATATAT
TCAAGAATGTTGAGGTTGTT

Downstream 100 bases:

>100_bases
TCTAAAAGATGGCCGGTTCAGTGTTTTCTGATCCGGCTATCTAACTCTCTGAATTATAATCACTGGTTGTTAATTTCGCA
TTTTAGTTTGGATGCTTGTT

Product: beta-lactamase AmpS

Products: NA

Alternate protein names: Penicillinase [H]

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MKKIIIFLCTGLLFCVSTWAQGTCFLAKENQTVLKREGNDCDQRYSPASTFKIALSLMGFDSGILKDELHPEWPYKKEYE
LYLNVWKYPQNPHTWIRDSCVWYSQALTRQLGMKRFKGYVDAFHYGNQDVSGDKGQNNGLTHAWLSSSLSISPTEQIQFL
QKIIYKKLPVSQKAYTMTKNIMYIQELPGGWKLYGKTGTGQQLTKDKSQKLPLQHGWFVGWIEKDERVITFAKHIADSKE
NTTFASFRAKNDTLIQLFNLINELEK

Sequences:

>Translated_266_residues
MKKIIIFLCTGLLFCVSTWAQGTCFLAKENQTVLKREGNDCDQRYSPASTFKIALSLMGFDSGILKDELHPEWPYKKEYE
LYLNVWKYPQNPHTWIRDSCVWYSQALTRQLGMKRFKGYVDAFHYGNQDVSGDKGQNNGLTHAWLSSSLSISPTEQIQFL
QKIIYKKLPVSQKAYTMTKNIMYIQELPGGWKLYGKTGTGQQLTKDKSQKLPLQHGWFVGWIEKDERVITFAKHIADSKE
NTTFASFRAKNDTLIQLFNLINELEK
>Mature_266_residues
MKKIIIFLCTGLLFCVSTWAQGTCFLAKENQTVLKREGNDCDQRYSPASTFKIALSLMGFDSGILKDELHPEWPYKKEYE
LYLNVWKYPQNPHTWIRDSCVWYSQALTRQLGMKRFKGYVDAFHYGNQDVSGDKGQNNGLTHAWLSSSLSISPTEQIQFL
QKIIYKKLPVSQKAYTMTKNIMYIQELPGGWKLYGKTGTGQQLTKDKSQKLPLQHGWFVGWIEKDERVITFAKHIADSKE
NTTFASFRAKNDTLIQLFNLINELEK

Specific function: Has a broad substrate profile, hydrolyzes amoxicillin, ticarcillin, cephalothin, ceftazidime, cefotaxime, and aztreonam, but not imipenem or cephamycins [H]

COG id: COG2602

COG function: function code V; Beta-lactamase class D

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-D beta-lactamase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR002137
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00905 Transpeptidase [H]

EC number: =3.5.2.6 [H]

Molecular weight: Translated: 30775; Mature: 30775

Theoretical pI: Translated: 9.48; Mature: 9.48

Prosite motif: PS00337 BETA_LACTAMASE_D

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIIIFLCTGLLFCVSTWAQGTCFLAKENQTVLKREGNDCDQRYSPASTFKIALSLMGF
CCEEEHHHHHHHHHHHHHHCCCEEEEEECCCHHHHCCCCCHHHHCCCHHHHHHHHHHHCC
DSGILKDELHPEWPYKKEYELYLNVWKYPQNPHTWIRDSCVWYSQALTRQLGMKRFKGYV
CCCCHHCCCCCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
DAFHYGNQDVSGDKGQNNGLTHAWLSSSLSISPTEQIQFLQKIIYKKLPVSQKAYTMTKN
HHHHCCCCCCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHH
IMYIQELPGGWKLYGKTGTGQQLTKDKSQKLPLQHGWFVGWIEKDERVITFAKHIADSKE
EEEEEECCCCEEEEECCCCCCHHHCCHHHCCCCCCCCEEEEEECCCHHHHHHHHHCCCCC
NTTFASFRAKNDTLIQLFNLINELEK
CCEEEEEECCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKIIIFLCTGLLFCVSTWAQGTCFLAKENQTVLKREGNDCDQRYSPASTFKIALSLMGF
CCEEEHHHHHHHHHHHHHHCCCEEEEEECCCHHHHCCCCCHHHHCCCHHHHHHHHHHHCC
DSGILKDELHPEWPYKKEYELYLNVWKYPQNPHTWIRDSCVWYSQALTRQLGMKRFKGYV
CCCCHHCCCCCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
DAFHYGNQDVSGDKGQNNGLTHAWLSSSLSISPTEQIQFLQKIIYKKLPVSQKAYTMTKN
HHHHCCCCCCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHH
IMYIQELPGGWKLYGKTGTGQQLTKDKSQKLPLQHGWFVGWIEKDERVITFAKHIADSKE
EEEEEECCCCEEEEECCCCCCHHHCCHHHCCCCCCCCEEEEEECCCHHHHHHHHHCCCCC
NTTFASFRAKNDTLIQLFNLINELEK
CCEEEEEECCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9333046 [H]