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The map label for this gene is artJ [H]

Identifier: 148359089

GI number: 148359089

Start: 1785396

End: 1786112

Strand: Reverse

Name: artJ [H]

Synonym: LPC_0977

Alternate gene names: 148359089

Gene position: 1786112-1785396 (Counterclockwise)

Preceding gene: 148359090

Following gene: 148359087

Centisome position: 49.94

GC content: 31.8

Gene sequence:

>717_bases
ATGAAGTTACTACAACTTCTCTTATTTATTATTATTTCCTCTAATTCTTATTCATTAAATTTAACAATTGGTACTTCAAA
ATTTAATCCGCCTTTTGAAGTTTGGTCTGGTAACAACAGTTCATTGTATGGCTTTGATATTGACCTTATGCAAGAAATAT
GCAGAAGATTACATGCGACCTGTACTTTTGAAGCCTATATATTCGATGATTTATTTTCCGCATTAAAAAACCGCGAAGTC
GATCTGGTTATTGCCTCAATGATTATTACAGATGAAAGAAAGAAACACTTTATTTTTAGTCTTCCCTATATGGAAAGTAA
CTCACAATATATAACTACCGTCGATTCTAAAATCAGCACATTTGATGATTTGCACGGCAAAAAAATTGGAGTAAGAAAAG
GAACTCCTTACGCAAGGCAAGTTTTGTCAGAAAACCGCAACAATCAGGTAATTTTTTACGAGCTCATACAAGATATGCTT
CTTGGCTTAAGCAATAATGAAGTTGATGCCAGTTTAATGGATTATGAAGCAGCAAAATATTGGATGGCATCTGAACCCTA
TGCTTATAAATTAATTGGTAAGAAAATTTCTATTGGAGAAGGGTATTCTATTATGGCAAACCCTGATCAATTTGTATTAA
TTAAAAAAATAAATAAAATTCTTCTTGAAATGGAAGCTGATGGAACTTATCTGAGACTATATTCAGAGTACTTTTAG

Upstream 100 bases:

>100_bases
AAACCAAATTAAAGAAATCGTATGATACAAATTGTACAAATCAAATTTTTTGTACCATGATTATGTTTAAACAAACTTCC
TAAATTACAGGATATAGTTT

Downstream 100 bases:

>100_bases
AAGAGGAGTATTAAGGACAAATAGCACCCAAAATACTCCAATTATACCTAAACAGGCTCCATTGTTTTTTTATTGGCCGA
GCTTTTTTGTTCCTTTTCTT

Product: arginine transport system periplasmic binding protein

Products: ADP; phosphate; L-arginine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MKLLQLLLFIIISSNSYSLNLTIGTSKFNPPFEVWSGNNSSLYGFDIDLMQEICRRLHATCTFEAYIFDDLFSALKNREV
DLVIASMIITDERKKHFIFSLPYMESNSQYITTVDSKISTFDDLHGKKIGVRKGTPYARQVLSENRNNQVIFYELIQDML
LGLSNNEVDASLMDYEAAKYWMASEPYAYKLIGKKISIGEGYSIMANPDQFVLIKKINKILLEMEADGTYLRLYSEYF

Sequences:

>Translated_238_residues
MKLLQLLLFIIISSNSYSLNLTIGTSKFNPPFEVWSGNNSSLYGFDIDLMQEICRRLHATCTFEAYIFDDLFSALKNREV
DLVIASMIITDERKKHFIFSLPYMESNSQYITTVDSKISTFDDLHGKKIGVRKGTPYARQVLSENRNNQVIFYELIQDML
LGLSNNEVDASLMDYEAAKYWMASEPYAYKLIGKKISIGEGYSIMANPDQFVLIKKINKILLEMEADGTYLRLYSEYF
>Mature_238_residues
MKLLQLLLFIIISSNSYSLNLTIGTSKFNPPFEVWSGNNSSLYGFDIDLMQEICRRLHATCTFEAYIFDDLFSALKNREV
DLVIASMIITDERKKHFIFSLPYMESNSQYITTVDSKISTFDDLHGKKIGVRKGTPYARQVLSENRNNQVIFYELIQDML
LGLSNNEVDASLMDYEAAKYWMASEPYAYKLIGKKISIGEGYSIMANPDQFVLIKKINKILLEMEADGTYLRLYSEYF

Specific function: Part of the ABC transporter complex ArtPIQMJ involved in arginine transport. Binds L-arginine with high affinity [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787085, Length=239, Percent_Identity=32.6359832635983, Blast_Score=142, Evalue=2e-35,
Organism=Escherichia coli, GI1787088, Length=213, Percent_Identity=33.8028169014084, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1788649, Length=249, Percent_Identity=31.3253012048193, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1788648, Length=232, Percent_Identity=28.8793103448276, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI1788228, Length=227, Percent_Identity=29.9559471365639, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1787031, Length=240, Percent_Identity=26.6666666666667, Blast_Score=82, Evalue=4e-17,

Paralogues:

None

Copy number: 1180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 27433; Mature: 27433

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLLQLLLFIIISSNSYSLNLTIGTSKFNPPFEVWSGNNSSLYGFDIDLMQEICRRLHAT
CHHHHHHHHHHCCCCCEEEEEEEECCCCCCCEEEECCCCCEEEEECHHHHHHHHHHHCCC
CTFEAYIFDDLFSALKNREVDLVIASMIITDERKKHFIFSLPYMESNSQYITTVDSKIST
EEEHHHHHHHHHHHHHCCCEEEEEEEHHHCCCCCCEEEEECCEECCCCCEEEEECCHHHH
FDDLHGKKIGVRKGTPYARQVLSENRNNQVIFYELIQDMLLGLSNNEVDASLMDYEAAKY
HHHCCCCEEECCCCCHHHHHHHHCCCCCEEEEHHHHHHHHHCCCCCCCCHHHHHHHHHHH
WMASEPYAYKLIGKKISIGEGYSIMANPDQFVLIKKINKILLEMEADGTYLRLYSEYF
CCCCCCEEEEEECCEEECCCCEEEEECCCCEEHHHHHHHHEEEECCCCCEEEEEECCC
>Mature Secondary Structure
MKLLQLLLFIIISSNSYSLNLTIGTSKFNPPFEVWSGNNSSLYGFDIDLMQEICRRLHAT
CHHHHHHHHHHCCCCCEEEEEEEECCCCCCCEEEECCCCCEEEEECHHHHHHHHHHHCCC
CTFEAYIFDDLFSALKNREVDLVIASMIITDERKKHFIFSLPYMESNSQYITTVDSKIST
EEEHHHHHHHHHHHHHCCCEEEEEEEHHHCCCCCCEEEEECCEECCCCCEEEEECCHHHH
FDDLHGKKIGVRKGTPYARQVLSENRNNQVIFYELIQDMLLGLSNNEVDASLMDYEAAKY
HHHCCCCEEECCCCCHHHHHHHHCCCCCEEEEHHHHHHHHHCCCCCCCCHHHHHHHHHHH
WMASEPYAYKLIGKKISIGEGYSIMANPDQFVLIKKINKILLEMEADGTYLRLYSEYF
CCCCCCEEEEEECCEEECCCCEEEEECCCCEEHHHHHHHHEEEECCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-arginine [Periplasm]; H2O [C]

Specific reaction: ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501075; 8905232; 9278503; 9298646; 9600841 [H]