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The map label for this gene is mdlC [H]

Identifier: 148358442

GI number: 148358442

Start: 325212

End: 326804

Strand: Reverse

Name: mdlC [H]

Synonym: LPC_0308

Alternate gene names: 148358442

Gene position: 326804-325212 (Counterclockwise)

Preceding gene: 148358443

Following gene: 148358440

Centisome position: 9.14

GC content: 39.05

Gene sequence:

>1593_bases
GTGAAGAAAACAGGAAGTGATGTTCTCAAAGAATTTATAACTACCTTTGAAATTAATTATATTTTTGGCAATCCTGGCAC
TACTGAATTAAAATTTCTGGAAGCGATTGAGCAATGTGATAATGCTACTTATTTTCTCACACTTCAAGAATCCAGTACCG
TAGGTATAGCAGCAGGCTATGCCATGATCACCAGGAAACCGGCTCTTATTAACTTACATAATTATGTTGGATTAGCGAAT
GCTGTCTGTAATATGTACAATGCATTCACTTCAGGAATTCCCCTGCTAATAATTGCTGGTCAACATTCACGTCCCTATTT
AATTCATAGCCCAGTCATGTCCGGAGATCTGACCTCTTTAGCGCAAACTGCAGTAAAATATGCCTATGAGGTGAATTCCG
CCAGCGATCTGGCTGTTGCTTTACAGCGTGGTTATGCTCAGGCAATTTTGCCTGGGCCAGGTCCGGTTTTTCTATCAATC
CCTATGGACATTTGGCAAGAGGAAACTCATGAAACCACGATAAAGAAGACAAGATTTTTACATGCTTGCATCAACGCTGA
AATAGAGCAAATTTGTGAGGTTTTAACAAATACCCCCAAAGGAAAATTGGCATTTATAGCGGATTATGAGGTAGGTGCAT
CGAATAGTATTTACCTTTTACATACCATTGCCGATCGATTAAACGCCGATATTTATGCTGCCCCCTATCATGTGCGTGAA
GTAATTAATCCCTTAAGTGTAAATTACAAAGGTAGATTGTCTGATAAATCTGGAGAAGTGCACAACCTGCTGCTGCAATA
TGAAACTGTGGTACTTTTAGGCGAAAAAGTGAAAGGGTATTTGTATACTGGTCAATCTTCTATTCCACAGGGTATACAAT
TCATACAAATCAGTGCCGCATCGGAACATCTGGCCTTTGACTATCCTTGCGATCTGGCAATTCTTGGCAATCTTGAGGCG
ACCTTAACTGCTATTAGCAAGCATTTAAAAATTATTGGGGTAAGTGTGCCGCTTAAACCAGCCACCAACATTAAAAATTT
GGAAAAAAAATATAAGCAACATCCTCTGCATTCGTTGATTGTTACCCTCCTGAATCAAGTAAATCGCTCTGTTCATTTAA
TAACAGAAGGATCATCGGAAGATGCTGTTGTGCAGGACATTGCAACAACACTAGGTTTTTTAAATGTGAATTTTTCACCC
AGAGGGGGTGGACTTGGCTGGGCTATGCCATTAGCAATAGGTATTTCTCTAGCGACCAGACACCATTCAATTTGTTTTGT
AGGTGATGGAGGCAGCATGTATGCGATTCATTCAATATGGTCAGCTGCCAAATATAAGATTCCTGTCATTTTCATTTGTT
TTGTTAATCGAGAATACAGAGTATTAAAAACAGATATGTCTCAAAATAGATCTTTCACAGAAGAAGATTTTTTTCGCCTG
GATTTGAATCATCCACCAATTGATGTCCAACAAATTGCCTTATCTTTCGGGGCACAGGTGAAGGCAATTAAATCAAAAGA
AGAAGTAGAGACTGTTCTCAAGAGTAGCTTTGCATTCCAAGGCCCAACATTTATTACCCTATATCATCAATAA

Upstream 100 bases:

>100_bases
ATTTGCCCTATTTTTAATGAATGAGAGAAATTGAACAAATAGCTCTCTTGTGCTAGGAATATAAATATGGAAGTGTTCCT
ATTTCTAAGGATGCTTATCG

Downstream 100 bases:

>100_bases
AACATTTCGAGAGCCAGACATTGGCTTTTGCATTATCTGAAAATGCAAAGCATAAAATCAGTCTCATTATTTAAAAATTT
GTTATTTTACAACTCATAGG

Product: benzoylformate decarboxylase

Products: NA

Alternate protein names: BFD; BFDC [H]

Number of amino acids: Translated: 530; Mature: 530

Protein sequence:

>530_residues
MKKTGSDVLKEFITTFEINYIFGNPGTTELKFLEAIEQCDNATYFLTLQESSTVGIAAGYAMITRKPALINLHNYVGLAN
AVCNMYNAFTSGIPLLIIAGQHSRPYLIHSPVMSGDLTSLAQTAVKYAYEVNSASDLAVALQRGYAQAILPGPGPVFLSI
PMDIWQEETHETTIKKTRFLHACINAEIEQICEVLTNTPKGKLAFIADYEVGASNSIYLLHTIADRLNADIYAAPYHVRE
VINPLSVNYKGRLSDKSGEVHNLLLQYETVVLLGEKVKGYLYTGQSSIPQGIQFIQISAASEHLAFDYPCDLAILGNLEA
TLTAISKHLKIIGVSVPLKPATNIKNLEKKYKQHPLHSLIVTLLNQVNRSVHLITEGSSEDAVVQDIATTLGFLNVNFSP
RGGGLGWAMPLAIGISLATRHHSICFVGDGGSMYAIHSIWSAAKYKIPVIFICFVNREYRVLKTDMSQNRSFTEEDFFRL
DLNHPPIDVQQIALSFGAQVKAIKSKEEVETVLKSSFAFQGPTFITLYHQ

Sequences:

>Translated_530_residues
MKKTGSDVLKEFITTFEINYIFGNPGTTELKFLEAIEQCDNATYFLTLQESSTVGIAAGYAMITRKPALINLHNYVGLAN
AVCNMYNAFTSGIPLLIIAGQHSRPYLIHSPVMSGDLTSLAQTAVKYAYEVNSASDLAVALQRGYAQAILPGPGPVFLSI
PMDIWQEETHETTIKKTRFLHACINAEIEQICEVLTNTPKGKLAFIADYEVGASNSIYLLHTIADRLNADIYAAPYHVRE
VINPLSVNYKGRLSDKSGEVHNLLLQYETVVLLGEKVKGYLYTGQSSIPQGIQFIQISAASEHLAFDYPCDLAILGNLEA
TLTAISKHLKIIGVSVPLKPATNIKNLEKKYKQHPLHSLIVTLLNQVNRSVHLITEGSSEDAVVQDIATTLGFLNVNFSP
RGGGLGWAMPLAIGISLATRHHSICFVGDGGSMYAIHSIWSAAKYKIPVIFICFVNREYRVLKTDMSQNRSFTEEDFFRL
DLNHPPIDVQQIALSFGAQVKAIKSKEEVETVLKSSFAFQGPTFITLYHQ
>Mature_530_residues
MKKTGSDVLKEFITTFEINYIFGNPGTTELKFLEAIEQCDNATYFLTLQESSTVGIAAGYAMITRKPALINLHNYVGLAN
AVCNMYNAFTSGIPLLIIAGQHSRPYLIHSPVMSGDLTSLAQTAVKYAYEVNSASDLAVALQRGYAQAILPGPGPVFLSI
PMDIWQEETHETTIKKTRFLHACINAEIEQICEVLTNTPKGKLAFIADYEVGASNSIYLLHTIADRLNADIYAAPYHVRE
VINPLSVNYKGRLSDKSGEVHNLLLQYETVVLLGEKVKGYLYTGQSSIPQGIQFIQISAASEHLAFDYPCDLAILGNLEA
TLTAISKHLKIIGVSVPLKPATNIKNLEKKYKQHPLHSLIVTLLNQVNRSVHLITEGSSEDAVVQDIATTLGFLNVNFSP
RGGGLGWAMPLAIGISLATRHHSICFVGDGGSMYAIHSIWSAAKYKIPVIFICFVNREYRVLKTDMSQNRSFTEEDFFRL
DLNHPPIDVQQIALSFGAQVKAIKSKEEVETVLKSSFAFQGPTFITLYHQ

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=563, Percent_Identity=19.7158081705151, Blast_Score=73, Evalue=7e-13,
Organism=Escherichia coli, GI87081685, Length=525, Percent_Identity=20.1904761904762, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1788716, Length=504, Percent_Identity=22.0238095238095, Blast_Score=80, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6323755, Length=161, Percent_Identity=26.0869565217391, Blast_Score=81, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =4.1.1.7 [H]

Molecular weight: Translated: 58435; Mature: 58435

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTGSDVLKEFITTFEINYIFGNPGTTELKFLEAIEQCDNATYFLTLQESSTVGIAAGY
CCCCHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHHHCCCEEEEEEECCCCEEEEECC
AMITRKPALINLHNYVGLANAVCNMYNAFTSGIPLLIIAGQHSRPYLIHSPVMSGDLTSL
HHHCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEECCCCCCCHHHH
AQTAVKYAYEVNSASDLAVALQRGYAQAILPGPGPVFLSIPMDIWQEETHETTIKKTRFL
HHHHHHHHHCCCCCHHHHHHHHCCCCEEECCCCCCEEEECCHHHHHCHHHHHHHHHHHHH
HACINAEIEQICEVLTNTPKGKLAFIADYEVGASNSIYLLHTIADRLNADIYAAPYHVRE
HHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCEEEEEEHHHHHCCCEEECCHHHHH
VINPLSVNYKGRLSDKSGEVHNLLLQYETVVLLGEKVKGYLYTGQSSIPQGIQFIQISAA
HHCCCCCCEEEECCCCCCHHHHHHHHHEEEEEEECCCCEEEEECCCCCCCCEEEEEEEEC
SEHLAFDYPCDLAILGNLEATLTAISKHLKIIGVSVPLKPATNIKNLEKKYKQHPLHSLI
CCCEEECCCCCEEEECCCHHHHHHHHHCEEEEEEEECCCCCCCHHHHHHHHHHCCHHHHH
VTLLNQVNRSVHLITEGSSEDAVVQDIATTLGFLNVNFSPRGGGLGWAMPLAIGISLATR
HHHHHHCCCEEEEEECCCCCCHHHHHHHHHHEEEEEEECCCCCCCCHHHHHHHHHHEEEC
HHSICFVGDGGSMYAIHSIWSAAKYKIPVIFICFVNREYRVLKTDMSQNRSFTEEDFFRL
CCEEEEEECCCCEEEHHHHHHCCCCCCCEEEEEEECCCEEEEECCHHCCCCCCCCCEEEE
DLNHPPIDVQQIALSFGAQVKAIKSKEEVETVLKSSFAFQGPTFITLYHQ
ECCCCCCHHHHHHHHHCCCEEECCCHHHHHHHHHHHCCCCCCEEEEEEEC
>Mature Secondary Structure
MKKTGSDVLKEFITTFEINYIFGNPGTTELKFLEAIEQCDNATYFLTLQESSTVGIAAGY
CCCCHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHHHCCCEEEEEEECCCCEEEEECC
AMITRKPALINLHNYVGLANAVCNMYNAFTSGIPLLIIAGQHSRPYLIHSPVMSGDLTSL
HHHCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEECCCCCCCHHHH
AQTAVKYAYEVNSASDLAVALQRGYAQAILPGPGPVFLSIPMDIWQEETHETTIKKTRFL
HHHHHHHHHCCCCCHHHHHHHHCCCCEEECCCCCCEEEECCHHHHHCHHHHHHHHHHHHH
HACINAEIEQICEVLTNTPKGKLAFIADYEVGASNSIYLLHTIADRLNADIYAAPYHVRE
HHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCEEEEEEHHHHHCCCEEECCHHHHH
VINPLSVNYKGRLSDKSGEVHNLLLQYETVVLLGEKVKGYLYTGQSSIPQGIQFIQISAA
HHCCCCCCEEEECCCCCCHHHHHHHHHEEEEEEECCCCEEEEECCCCCCCCEEEEEEEEC
SEHLAFDYPCDLAILGNLEATLTAISKHLKIIGVSVPLKPATNIKNLEKKYKQHPLHSLI
CCCEEECCCCCEEEECCCHHHHHHHHHCEEEEEEEECCCCCCCHHHHHHHHHHCCHHHHH
VTLLNQVNRSVHLITEGSSEDAVVQDIATTLGFLNVNFSPRGGGLGWAMPLAIGISLATR
HHHHHHCCCEEEEEECCCCCCHHHHHHHHHHEEEEEEECCCCCCCCHHHHHHHHHHEEEC
HHSICFVGDGGSMYAIHSIWSAAKYKIPVIFICFVNREYRVLKTDMSQNRSFTEEDFFRL
CCEEEEEECCCCEEEHHHHHHCCCCCCCEEEEEEECCCEEEEECCHHCCCCCCCCCEEEE
DLNHPPIDVQQIALSFGAQVKAIKSKEEVETVLKSSFAFQGPTFITLYHQ
ECCCCCCHHHHHHHHHCCCEEECCCHHHHHHHHHHHCCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2271624; 7663351; 9665697 [H]