Click here to switch to the map view.

The map label for this gene is ruvB

Identifier: 148268121

GI number: 148268121

Start: 1801073

End: 1802077

Strand: Reverse

Name: ruvB

Synonym: SaurJH9_1698

Alternate gene names: 148268121

Gene position: 1802077-1801073 (Counterclockwise)

Preceding gene: 148268122

Following gene: 148268120

Centisome position: 62.0

GC content: 37.11

Gene sequence:

>1005_bases
ATGAATGAGCGTATGGTTGATCAATCAATGCATAGTGAAGAAACTGATTTCGAATTGTCGCTTAGACCTACGAGATTACG
ACAATATATTGGTCAAAATTCAATAAAAAGTAATTTAGAAGTATTTATTAAAGCGGCTAAACTTCGTCATGAACCATTAG
ATCATGTATTGCTTTTTGGCCCCCCTGGATTAGGTAAGACAACATTATCTAATATCATTGCCAATGAAATGGAAGTTAAT
ATACGTACAGTATCAGGGCCTTCATTAGAAAGACCTGGTGATTTGGCTGCAATTTTATCAGGACTTCAACCTGGAGATGT
ATTGTTTATTGATGAAATACACAGACTGAGTAGTGTTGTTGAAGAAGTGTTATACCCTGCAATGGAAGATTTCTTTTTAG
ATATTATCATTGGTAAAGGCGATGAGGCTAGAAGTATCCGTATCGACTTACCTCCATTCACTTTGGTAGGTGCAACAACG
CGAGCTGGCAGCTTAACAGGTCCACTAAGGGATCGATTTGGTGTGCACTTAAGATTAGAATATTATAATGAATCAGATTT
AAAAGAAATCATTATTAGAACAGCTGAGGTTTTAGGCACAGGTATTGATGAAGAAAGTGCCATTGAACTTGCTAAACGTT
CTAGAGGGACTCCAAGAGTAGCAAATCGACTATTGAAGCGGGTAAGAGACTTCCAGCAAGTGAATGAAGATGAACAAATA
TACATTGAAACAACGAAGCACGCATTAGGTTTACTTCAAGTTGATCAACACGGACTAGATTACATTGATCATAAAATGAT
GAACTGTATTATTAAGCAGTATAATGGTGGACCTGTTGGTTTAGATACGATTGCCGTAACAATTGGTGAAGAACGTATTA
CAATTGAGGACGTTTATGAGCCATTTCTTATTCAGAAAGGCTTTTTAGAACGTACGCCACGTGGCAGAAAAGCAACACCA
TTAGCTTATGAACATTTTGCAAAGTCGAATGAGGAGAGAGGATAA

Upstream 100 bases:

>100_bases
TTAAATAAAAATAAATATGACTCAGTTGATGAAGCTGTTAAGGCAGGTCTTCAATTAGTTGTATCTTAATTTTAAATAGA
TTAATAGGGGAAGTGTTGTC

Downstream 100 bases:

>100_bases
CGTGAATATTGAAGAATTTGACTATGACTTACCAGAATCATTAATTGCTCAAACGCCTTTAAAAGATCGTGATCATAGTC
GTTTATTAGTCATGGATAGA

Product: Holliday junction DNA helicase RuvB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 334; Mature: 334

Protein sequence:

>334_residues
MNERMVDQSMHSEETDFELSLRPTRLRQYIGQNSIKSNLEVFIKAAKLRHEPLDHVLLFGPPGLGKTTLSNIIANEMEVN
IRTVSGPSLERPGDLAAILSGLQPGDVLFIDEIHRLSSVVEEVLYPAMEDFFLDIIIGKGDEARSIRIDLPPFTLVGATT
RAGSLTGPLRDRFGVHLRLEYYNESDLKEIIIRTAEVLGTGIDEESAIELAKRSRGTPRVANRLLKRVRDFQQVNEDEQI
YIETTKHALGLLQVDQHGLDYIDHKMMNCIIKQYNGGPVGLDTIAVTIGEERITIEDVYEPFLIQKGFLERTPRGRKATP
LAYEHFAKSNEERG

Sequences:

>Translated_334_residues
MNERMVDQSMHSEETDFELSLRPTRLRQYIGQNSIKSNLEVFIKAAKLRHEPLDHVLLFGPPGLGKTTLSNIIANEMEVN
IRTVSGPSLERPGDLAAILSGLQPGDVLFIDEIHRLSSVVEEVLYPAMEDFFLDIIIGKGDEARSIRIDLPPFTLVGATT
RAGSLTGPLRDRFGVHLRLEYYNESDLKEIIIRTAEVLGTGIDEESAIELAKRSRGTPRVANRLLKRVRDFQQVNEDEQI
YIETTKHALGLLQVDQHGLDYIDHKMMNCIIKQYNGGPVGLDTIAVTIGEERITIEDVYEPFLIQKGFLERTPRGRKATP
LAYEHFAKSNEERG
>Mature_334_residues
MNERMVDQSMHSEETDFELSLRPTRLRQYIGQNSIKSNLEVFIKAAKLRHEPLDHVLLFGPPGLGKTTLSNIIANEMEVN
IRTVSGPSLERPGDLAAILSGLQPGDVLFIDEIHRLSSVVEEVLYPAMEDFFLDIIIGKGDEARSIRIDLPPFTLVGATT
RAGSLTGPLRDRFGVHLRLEYYNESDLKEIIIRTAEVLGTGIDEESAIELAKRSRGTPRVANRLLKRVRDFQQVNEDEQI
YIETTKHALGLLQVDQHGLDYIDHKMMNCIIKQYNGGPVGLDTIAVTIGEERITIEDVYEPFLIQKGFLERTPRGRKATP
LAYEHFAKSNEERG

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG2255

COG function: function code L; Holliday junction resolvasome, helicase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvB family

Homologues:

Organism=Escherichia coli, GI1788167, Length=330, Percent_Identity=59.3939393939394, Blast_Score=410, Evalue=1e-116,
Organism=Escherichia coli, GI1787119, Length=230, Percent_Identity=26.0869565217391, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVB_STAA1 (A7X357)

Other databases:

- EMBL:   AP009324
- RefSeq:   YP_001442218.1
- ProteinModelPortal:   A7X357
- SMR:   A7X357
- STRING:   A7X357
- EnsemblBacteria:   EBSTAT00000004847
- GeneID:   5559627
- GenomeReviews:   AP009324_GR
- KEGG:   saw:SAHV_1628
- eggNOG:   COG2255
- GeneTree:   EBGT00050000025492
- HOGENOM:   HBG339960
- OMA:   EKFDGGP
- ProtClustDB:   PRK00080
- BioCyc:   SAUR418127:SAHV_1628-MONOMER
- HAMAP:   MF_00016
- InterPro:   IPR003593
- InterPro:   IPR004605
- InterPro:   IPR008823
- InterPro:   IPR008824
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- ProDom:   PD005323
- SMART:   SM00382
- TIGRFAMs:   TIGR00635

Pfam domain/function: PF05491 RuvB_C; PF05496 RuvB_N

EC number: =3.6.4.12

Molecular weight: Translated: 37647; Mature: 37647

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNERMVDQSMHSEETDFELSLRPTRLRQYIGQNSIKSNLEVFIKAAKLRHEPLDHVLLFG
CCCCHHHHHHCCCCCCEEEEECHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEEEC
PPGLGKTTLSNIIANEMEVNIRTVSGPSLERPGDLAAILSGLQPGDVLFIDEIHRLSSVV
CCCCCHHHHHHHHHHHHEEEEEEECCCCCCCCCHHHHHHHCCCCCCEEEHHHHHHHHHHH
EEVLYPAMEDFFLDIIIGKGDEARSIRIDLPPFTLVGATTRAGSLTGPLRDRFGVHLRLE
HHHHHHHHHHHEEEEEECCCCCCCEEEEECCCEEEEECCCCCCCCCCCHHHHCCEEEEEE
YYNESDLKEIIIRTAEVLGTGIDEESAIELAKRSRGTPRVANRLLKRVRDFQQVNEDEQI
ECCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCEE
YIETTKHALGLLQVDQHGLDYIDHKMMNCIIKQYNGGPVGLDTIAVTIGEERITIEDVYE
EEEEHHHHHHEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCEEEHHHHHH
PFLIQKGFLERTPRGRKATPLAYEHFAKSNEERG
HHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCC
>Mature Secondary Structure
MNERMVDQSMHSEETDFELSLRPTRLRQYIGQNSIKSNLEVFIKAAKLRHEPLDHVLLFG
CCCCHHHHHHCCCCCCEEEEECHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEEEC
PPGLGKTTLSNIIANEMEVNIRTVSGPSLERPGDLAAILSGLQPGDVLFIDEIHRLSSVV
CCCCCHHHHHHHHHHHHEEEEEEECCCCCCCCCHHHHHHHCCCCCCEEEHHHHHHHHHHH
EEVLYPAMEDFFLDIIIGKGDEARSIRIDLPPFTLVGATTRAGSLTGPLRDRFGVHLRLE
HHHHHHHHHHHEEEEEECCCCCCCEEEEECCCEEEEECCCCCCCCCCCHHHHCCEEEEEE
YYNESDLKEIIIRTAEVLGTGIDEESAIELAKRSRGTPRVANRLLKRVRDFQQVNEDEQI
ECCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCEE
YIETTKHALGLLQVDQHGLDYIDHKMMNCIIKQYNGGPVGLDTIAVTIGEERITIEDVYE
EEEEHHHHHHEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCEEEHHHHHH
PFLIQKGFLERTPRGRKATPLAYEHFAKSNEERG
HHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA