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The map label for this gene is queA

Identifier: 148268120

GI number: 148268120

Start: 1800046

End: 1801071

Strand: Reverse

Name: queA

Synonym: SaurJH9_1697

Alternate gene names: 148268120

Gene position: 1801071-1800046 (Counterclockwise)

Preceding gene: 148268121

Following gene: 148268119

Centisome position: 61.96

GC content: 33.53

Gene sequence:

>1026_bases
GTGAATATTGAAGAATTTGACTATGACTTACCAGAATCATTAATTGCTCAAACGCCTTTAAAAGATCGTGATCATAGTCG
TTTATTAGTCATGGATAGAGAAACTGGTGAAATGAAACATTTACATTTCAAAGATATCATTGAGTATTTTAGACCTGGTG
ATACATTAGTGCTTAACGATACGCGAGTAATGCCAGCTAGACTTTTTGGTTTAAAAGAAGAAACTGGTGCAAAAGTTGAA
ATGTTAATGTTAACTCAAATTGAAGGTAATGATTGGGAAGTCTTACTGAAACCAGCTAAGCGTATTAAAGTTGGTAATAA
ATTGAATTTTGGTAATGGCAAAATTATAGCTGAGTGCATAAAAGAAATGGATCAAGGTGGACGCATCATGCGTTTACATT
ATGAAGGTATTTTACAAGAAAGATTAGATGAATTAGGGGAAATGCCACTGCCACCATACATCAAAGAACGTTTAGATGAT
CCAGATCGTTATCAAACAGTTTACGCTAAAGAAAGTGGTTCAGCGGCAGCACCAACAGCAGGATTACATTTTACTGATGA
GTTATTAACTGAAATTAAAAATAAAGGCGTTAATATCGCATTTGTTACATTACATGTTGGGTTAGGTACGTTTAGACCGG
TGAGCGTCGATGATGTGAATGACCACGAAATGCATAGTGAATATTATCAAATGACACAAGAAACAGCTGATTTATTAAAT
GATACTAAGTCCAAAGGACATCGCATTATATCAGTTGGTACAACTTCAACACGTACACTTGAAACAATTCGACGCGATCA
TGATAAATTTGTTGAAACGAGTGGCTGGACTAATATATTTATTTATCCAGGATTTGATTTTAAAGCAATTGATGGCCAGA
TTACTAATTTTCATTTACCAAAATCAACATTAGTTATGCTAGTATCAGCGTTTAGTACTCGTGAAAATGTTCTGAATGCT
TATAAAACAGCAGTAAATTTAGAATATAGATTCTTTAGTTTTGGCGATGCAATGTTAATTATATAA

Upstream 100 bases:

>100_bases
CTTATTCAGAAAGGCTTTTTAGAACGTACGCCACGTGGCAGAAAAGCAACACCATTAGCTTATGAACATTTTGCAAAGTC
GAATGAGGAGAGAGGATAAC

Downstream 100 bases:

>100_bases
AAAGAATGTGAGGATTTTGAATATGCCTGCAGTAACATACGAACACATTAAAACTTGTAAACAATCAGGTGCGCGTTTAG
GTATCGTGCACACACCACAC

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MNIEEFDYDLPESLIAQTPLKDRDHSRLLVMDRETGEMKHLHFKDIIEYFRPGDTLVLNDTRVMPARLFGLKEETGAKVE
MLMLTQIEGNDWEVLLKPAKRIKVGNKLNFGNGKIIAECIKEMDQGGRIMRLHYEGILQERLDELGEMPLPPYIKERLDD
PDRYQTVYAKESGSAAAPTAGLHFTDELLTEIKNKGVNIAFVTLHVGLGTFRPVSVDDVNDHEMHSEYYQMTQETADLLN
DTKSKGHRIISVGTTSTRTLETIRRDHDKFVETSGWTNIFIYPGFDFKAIDGQITNFHLPKSTLVMLVSAFSTRENVLNA
YKTAVNLEYRFFSFGDAMLII

Sequences:

>Translated_341_residues
MNIEEFDYDLPESLIAQTPLKDRDHSRLLVMDRETGEMKHLHFKDIIEYFRPGDTLVLNDTRVMPARLFGLKEETGAKVE
MLMLTQIEGNDWEVLLKPAKRIKVGNKLNFGNGKIIAECIKEMDQGGRIMRLHYEGILQERLDELGEMPLPPYIKERLDD
PDRYQTVYAKESGSAAAPTAGLHFTDELLTEIKNKGVNIAFVTLHVGLGTFRPVSVDDVNDHEMHSEYYQMTQETADLLN
DTKSKGHRIISVGTTSTRTLETIRRDHDKFVETSGWTNIFIYPGFDFKAIDGQITNFHLPKSTLVMLVSAFSTRENVLNA
YKTAVNLEYRFFSFGDAMLII
>Mature_341_residues
MNIEEFDYDLPESLIAQTPLKDRDHSRLLVMDRETGEMKHLHFKDIIEYFRPGDTLVLNDTRVMPARLFGLKEETGAKVE
MLMLTQIEGNDWEVLLKPAKRIKVGNKLNFGNGKIIAECIKEMDQGGRIMRLHYEGILQERLDELGEMPLPPYIKERLDD
PDRYQTVYAKESGSAAAPTAGLHFTDELLTEIKNKGVNIAFVTLHVGLGTFRPVSVDDVNDHEMHSEYYQMTQETADLLN
DTKSKGHRIISVGTTSTRTLETIRRDHDKFVETSGWTNIFIYPGFDFKAIDGQITNFHLPKSTLVMLVSAFSTRENVLNA
YKTAVNLEYRFFSFGDAMLII

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA)

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family

Homologues:

Organism=Escherichia coli, GI1786606, Length=344, Percent_Identity=45.3488372093023, Blast_Score=304, Evalue=6e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEA_STAA1 (A7X356)

Other databases:

- EMBL:   AP009324
- RefSeq:   YP_001442217.1
- ProteinModelPortal:   A7X356
- SMR:   A7X356
- STRING:   A7X356
- EnsemblBacteria:   EBSTAT00000005299
- GeneID:   5560968
- GenomeReviews:   AP009324_GR
- KEGG:   saw:SAHV_1627
- eggNOG:   COG0809
- GeneTree:   EBGT00050000024968
- HOGENOM:   HBG286076
- OMA:   SEFYRLT
- ProtClustDB:   PRK00147
- BioCyc:   SAUR418127:SAHV_1627-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00113
- InterPro:   IPR003699
- TIGRFAMs:   TIGR00113

Pfam domain/function: PF02547 Queuosine_synth; SSF111337 Queuosine_synth

EC number: 5.-.-.- [C]

Molecular weight: Translated: 38971; Mature: 38971

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIEEFDYDLPESLIAQTPLKDRDHSRLLVMDRETGEMKHLHFKDIIEYFRPGDTLVLND
CCCCCCCCCCCHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHCCCCEEEEEC
TRVMPARLFGLKEETGAKVEMLMLTQIEGNDWEVLLKPAKRIKVGNKLNFGNGKIIAECI
CCCCHHHHHCCCCCCCCEEEEEEEEEECCCCEEEEECCHHHEECCCEEECCCCHHHHHHH
KEMDQGGRIMRLHYEGILQERLDELGEMPLPPYIKERLDDPDRYQTVYAKESGSAAAPTA
HHHHCCCCEEEEEHHHHHHHHHHHHCCCCCCHHHHHHCCCCCHHEEEEEECCCCCCCCCC
GLHFTDELLTEIKNKGVNIAFVTLHVGLGTFRPVSVDDVNDHEMHSEYYQMTQETADLLN
CCCCHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
DTKSKGHRIISVGTTSTRTLETIRRDHDKFVETSGWTNIFIYPGFDFKAIDGQITNFHLP
HHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEECCCEEEEEECC
KSTLVMLVSAFSTRENVLNAYKTAVNLEYRFFSFGDAMLII
HHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEECCEEEEC
>Mature Secondary Structure
MNIEEFDYDLPESLIAQTPLKDRDHSRLLVMDRETGEMKHLHFKDIIEYFRPGDTLVLND
CCCCCCCCCCCHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHCCCCEEEEEC
TRVMPARLFGLKEETGAKVEMLMLTQIEGNDWEVLLKPAKRIKVGNKLNFGNGKIIAECI
CCCCHHHHHCCCCCCCCEEEEEEEEEECCCCEEEEECCHHHEECCCEEECCCCHHHHHHH
KEMDQGGRIMRLHYEGILQERLDELGEMPLPPYIKERLDDPDRYQTVYAKESGSAAAPTA
HHHHCCCCEEEEEHHHHHHHHHHHHCCCCCCHHHHHHCCCCCHHEEEEEECCCCCCCCCC
GLHFTDELLTEIKNKGVNIAFVTLHVGLGTFRPVSVDDVNDHEMHSEYYQMTQETADLLN
CCCCHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
DTKSKGHRIISVGTTSTRTLETIRRDHDKFVETSGWTNIFIYPGFDFKAIDGQITNFHLP
HHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEECCCEEEEEECC
KSTLVMLVSAFSTRENVLNAYKTAVNLEYRFFSFGDAMLII
HHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEECCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA