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The map label for this gene is trmFO [H]

Identifier: 148267741

GI number: 148267741

Start: 1364002

End: 1365309

Strand: Direct

Name: trmFO [H]

Synonym: SaurJH9_1311

Alternate gene names: 148267741

Gene position: 1364002-1365309 (Clockwise)

Preceding gene: 148267740

Following gene: 148267742

Centisome position: 46.93

GC content: 35.55

Gene sequence:

>1308_bases
ATGACTCAAACTGTAAATGTAATAGGTGCTGGTCTTGCCGGTTCAGAAGCGGCATATCAATTAGCTGAAAGAGGAATTAA
AGTTAATCTAATAGAGATGAGACCTGTTAAACAAACACCAGCGCACCATACTGATAAATTTGCGGAACTTGTATGTTCCA
ATTCATTACGCGGAAATGCTTTAACTAATGGTGTGGGTGTTTTAAAAGAAGAAATGAGAAGATTGAATTCTATAATTATT
GAAGCGGCTGATAAGGCACGAGTTCCAGCTGGTGGTGCATTAGCAGTTGATAGACACGATTTTTCAGGTTATATTACTGA
AACACTTAAAAATCATGAAAATATCACAGTTATTAATGAAGAAATTAATGCCATTCCAGATGGATACACAATTATCGCAA
CAGGACCACTTACTACAGAAACCCTTGCGCAAGAAATAGTGGACATTACTGGTAAAGATCAACTTTATTTCTATGATGCA
GCTGCTCCAATTATTGAAAAAGAATCTATTGATATGGATAAAGTTTACTTAAAGTCCCGTTATGATAAAGGTGAAGCTGC
ATATTTAAACTGTCCTATGACTGAGGATGAATTTAATCGCTTTTATGATGCAGTATTAGAAGCTGAAGTTGCACCTGTAA
ATTCATTTGAAAAAGAAAAATATTTCGAGGGTTGTATGCCTTTTGAAGTAATGGCAGAACGCGGGCGCAAGACATTACTA
TTTGGACCAATGAAACCAGTAGGATTAGAAGATCCAAAGACTGGGAAACGTCCTTATGCAGTGGTTCAATTAAGACAAGA
TGACGCTGCTGGCACACTCTACAATATTGTCGGCTTCCAAACACATTTAAAATGGGGAGCTCAAAAAGAAGTCATTAAAT
TAATTCCAGGTTTAGAGAATGTTGATATTGTTAGATATGGTGTGATGCATAGAAATACCTTCATTAACTCACCGGACGTA
TTAAACGAAAAATATGAATTGATTTCACAACCAAACATACAGTTTGCGGGACAAATGACTGGTGTTGAAGGTTATGTAGA
AAGCGCAGCTAGCGGCTTAGTTGCAGGTATCAATCTTGCGCATAAAATATTAGGCAAAGGTGAGGTAGTATTTCCGAGAG
AAACTATGATTGGAAGTATGGCTTACTATATTTCTCATGCTAAAAACAATAAGAATTTCCAACCTATGAATGCTAACTTC
GGGTTATTACCATCTTTAGAAACTAGAATTAAAGATAAAAAAGAACGCTATGAAGCACAAGCTAATAGAGCTTTGGATTA
CTTAAAAAATTTCAAAAAAACTTTATAA

Upstream 100 bases:

>100_bases
TTATTTTAAAGATAGTAATGGATTAATCTAAATAAGTGCGGATAATATAAACATAACAACATAATTAAAAGACATAAATG
ACAATAAAAGGAGTATAGAA

Downstream 100 bases:

>100_bases
AATAGTTAGAAAGACTAGATATGCTATTCATTCTTAAGTCATCAACGAGTAAGTAATGACTTTCTAAATGGAAAATACTT
ATCCTAGTCTTTTTAATTTT

Product: tRNA (uracil-5-)-methyltransferase Gid

Products: NA

Alternate protein names: Folate-dependent tRNA (uracil-5-)-methyltransferase; Folate-dependent tRNA(M-5-U54)-methyltransferase [H]

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MTQTVNVIGAGLAGSEAAYQLAERGIKVNLIEMRPVKQTPAHHTDKFAELVCSNSLRGNALTNGVGVLKEEMRRLNSIII
EAADKARVPAGGALAVDRHDFSGYITETLKNHENITVINEEINAIPDGYTIIATGPLTTETLAQEIVDITGKDQLYFYDA
AAPIIEKESIDMDKVYLKSRYDKGEAAYLNCPMTEDEFNRFYDAVLEAEVAPVNSFEKEKYFEGCMPFEVMAERGRKTLL
FGPMKPVGLEDPKTGKRPYAVVQLRQDDAAGTLYNIVGFQTHLKWGAQKEVIKLIPGLENVDIVRYGVMHRNTFINSPDV
LNEKYELISQPNIQFAGQMTGVEGYVESAASGLVAGINLAHKILGKGEVVFPRETMIGSMAYYISHAKNNKNFQPMNANF
GLLPSLETRIKDKKERYEAQANRALDYLKNFKKTL

Sequences:

>Translated_435_residues
MTQTVNVIGAGLAGSEAAYQLAERGIKVNLIEMRPVKQTPAHHTDKFAELVCSNSLRGNALTNGVGVLKEEMRRLNSIII
EAADKARVPAGGALAVDRHDFSGYITETLKNHENITVINEEINAIPDGYTIIATGPLTTETLAQEIVDITGKDQLYFYDA
AAPIIEKESIDMDKVYLKSRYDKGEAAYLNCPMTEDEFNRFYDAVLEAEVAPVNSFEKEKYFEGCMPFEVMAERGRKTLL
FGPMKPVGLEDPKTGKRPYAVVQLRQDDAAGTLYNIVGFQTHLKWGAQKEVIKLIPGLENVDIVRYGVMHRNTFINSPDV
LNEKYELISQPNIQFAGQMTGVEGYVESAASGLVAGINLAHKILGKGEVVFPRETMIGSMAYYISHAKNNKNFQPMNANF
GLLPSLETRIKDKKERYEAQANRALDYLKNFKKTL
>Mature_434_residues
TQTVNVIGAGLAGSEAAYQLAERGIKVNLIEMRPVKQTPAHHTDKFAELVCSNSLRGNALTNGVGVLKEEMRRLNSIIIE
AADKARVPAGGALAVDRHDFSGYITETLKNHENITVINEEINAIPDGYTIIATGPLTTETLAQEIVDITGKDQLYFYDAA
APIIEKESIDMDKVYLKSRYDKGEAAYLNCPMTEDEFNRFYDAVLEAEVAPVNSFEKEKYFEGCMPFEVMAERGRKTLLF
GPMKPVGLEDPKTGKRPYAVVQLRQDDAAGTLYNIVGFQTHLKWGAQKEVIKLIPGLENVDIVRYGVMHRNTFINSPDVL
NEKYELISQPNIQFAGQMTGVEGYVESAASGLVAGINLAHKILGKGEVVFPRETMIGSMAYYISHAKNNKNFQPMNANFG
LLPSLETRIKDKKERYEAQANRALDYLKNFKKTL

Specific function: Catalyzes the folate-dependent formation of 5-methyl- uridine at position 54 (M-5-U54) in all tRNAs [H]

COG id: COG1206

COG function: function code J; NAD(FAD)-utilizing enzyme possibly involved in translation

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family. TrmFO subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004417
- InterPro:   IPR002218
- InterPro:   IPR020595 [H]

Pfam domain/function: PF01134 GIDA [H]

EC number: =2.1.1.74 [H]

Molecular weight: Translated: 48371; Mature: 48240

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQTVNVIGAGLAGSEAAYQLAERGIKVNLIEMRPVKQTPAHHTDKFAELVCSNSLRGNA
CCCEEEEEECCCCCCHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCC
LTNGVGVLKEEMRRLNSIIIEAADKARVPAGGALAVDRHDFSGYITETLKNHENITVINE
HHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCEEEEECCCCCHHHHHHHCCCCCEEEECC
EINAIPDGYTIIATGPLTTETLAQEIVDITGKDQLYFYDAAAPIIEKESIDMDKVYLKSR
HHCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHC
YDKGEAAYLNCPMTEDEFNRFYDAVLEAEVAPVNSFEKEKYFEGCMPFEVMAERGRKTLL
CCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCHHHHHHCCCEEEE
FGPMKPVGLEDPKTGKRPYAVVQLRQDDAAGTLYNIVGFQTHLKWGAQKEVIKLIPGLEN
ECCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHCCCCCC
VDIVRYGVMHRNTFINSPDVLNEKYELISQPNIQFAGQMTGVEGYVESAASGLVAGINLA
CCEEEEEHHHCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHH
HKILGKGEVVFPRETMIGSMAYYISHAKNNKNFQPMNANFGLLPSLETRIKDKKERYEAQ
HHHHCCCCEEECHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
ANRALDYLKNFKKTL
HHHHHHHHHHHHHCC
>Mature Secondary Structure 
TQTVNVIGAGLAGSEAAYQLAERGIKVNLIEMRPVKQTPAHHTDKFAELVCSNSLRGNA
CCEEEEEECCCCCCHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCC
LTNGVGVLKEEMRRLNSIIIEAADKARVPAGGALAVDRHDFSGYITETLKNHENITVINE
HHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCEEEEECCCCCHHHHHHHCCCCCEEEECC
EINAIPDGYTIIATGPLTTETLAQEIVDITGKDQLYFYDAAAPIIEKESIDMDKVYLKSR
HHCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHC
YDKGEAAYLNCPMTEDEFNRFYDAVLEAEVAPVNSFEKEKYFEGCMPFEVMAERGRKTLL
CCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCHHHHHHCCCEEEE
FGPMKPVGLEDPKTGKRPYAVVQLRQDDAAGTLYNIVGFQTHLKWGAQKEVIKLIPGLEN
ECCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHCCCCCC
VDIVRYGVMHRNTFINSPDVLNEKYELISQPNIQFAGQMTGVEGYVESAASGLVAGINLA
CCEEEEEHHHCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHH
HKILGKGEVVFPRETMIGSMAYYISHAKNNKNFQPMNANFGLLPSLETRIKDKKERYEAQ
HHHHCCCCEEECHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
ANRALDYLKNFKKTL
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA