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The map label for this gene is topA [H]

Identifier: 148267740

GI number: 148267740

Start: 1361771

End: 1363846

Strand: Direct

Name: topA [H]

Synonym: SaurJH9_1310

Alternate gene names: 148267740

Gene position: 1361771-1363846 (Clockwise)

Preceding gene: 148267739

Following gene: 148267741

Centisome position: 46.85

GC content: 35.12

Gene sequence:

>2076_bases
ATGACATTGGCAGATAATTTAGTCATTGTTGAATCGCCTGCAAAAGCAAAAACCATTGAAAAGTATTTAGGTAAGAAATA
TAAAGTTATAGCTTCAATGGGACACGTCAGAGACTTACCAAGAAGTCAAATGGGTGTCGACACTGAAGATAATTACGAAC
CAAAATATATAACAATACGCGGAAAAGGTCCTGTTGTAAAAGAATTGAAAAAACATGCAAAAAAAGCGAAAAACGTCTTT
CTCGCAAGTGACCCCGACCGTGAAGGTGAAGCAATTGCTTGGCATTTATCAAAAATTTTAGAGCTTGAAGATTCTAAAGA
AAATCGCGTTGTTTTCAACGAAATAACTAAAGACGCTGTTAAAGAAAGTTTTAAAAATCCTAGAGAAATTGAAATGAACT
TAGTCGATGCACAACAAGCGCGTCGAATATTAGATAGATTGGTTGGCTATAACATCTCGCCAGTTCTATGGAAAAAAGTG
AAAAAAGGTTTGTCAGCGGGTCGAGTTCAATCTGTTGCACTTCGTTTAGTCATTGACCGTGAAAATGAAATTCGAAACTT
TAAACCAGAAGAATATTGGACTATTGAAGGAGAATTTAGATACAAAAAATCAAAATTCAATGCTAAATTCCTTCATTATA
AAAATAAACCTTTTAAATTAAAAACGAAAAAAGATGTTGAGAAAATTACAGCTGCACTAGATGGAGATCAATTCGAAATT
ACAAACGTGACTAAAAAAGAAAAAACGCGTAATCCAGCAAACCCATTTACAACTTCTACATTACAACAAGAGGCGGCACG
TAAATTAAACTTTAAAGCAAGAAAAACAATGATGGTCGCACAACAATTATATGAAGGTATAGATTTGAAAAAACAAGGTA
CGATTGGTTTAATAACATATATGAGAACCGATTCTACACGTATTTCAGATACTGCCAAAGCTGAAGCAAAACAGTATATA
ACTGATAAATACGGTGAATCTTACACTTCTAAACGTAAAGCATCAGGGAAACAAGGTGACCAAGATGCCCATGAGGCTAT
TAGACCTTCAAGTACTATGCGTACGCCAGATGATATGAAGTCATTTTTGACGAAAGACCAATACCGATTATACAAATTAA
TTTGGGAACGATTTGTTGCTAGTCAAATGGCTCCAGCAATACTTGATACAGTCTCATTAGACATAACACAAGGTGACATT
AAATTTAGAGCGAATGGTCAAACAATCAAGTTTAAAGGATTTATGACACTTTATGTAGAAACTAAAGATGATAGTGATAG
CGAAAAGGAAAATAAACTGCCTAAATTAGAGCAAGGTGATAAAGTCACAGCAACTCAAATTGAACCAGCTCAACACTATA
CACAACCACCTCCAAGATATACTGAGGCGAGATTAGTAAAAACACTAGAAGAATTGAAAATTGGGCGACCATCAACTTAT
GCACCGACAATAGATACGATTCAAAAGCGTAACTATGTCAAATTAGAAAGTAAGCGTTTTGTTCCTACTGAGTTGGGAGA
AATAGTTCATGAACAAGTGAAAGAATACTTCCCAGAGATTATTGATGTGGAATTCACAGTGAATATGGAAACGTTACTTG
ATAAGATTGCAGAAGGCGACATTACATGGAGAAAAGTAATAGACGGTTTCTTTAGTAGCTTTAAACAAGATGTTGAACGT
GCTGAAGAAGAGATGGAAAAGATTGAAATCAAAGATGAGCCAGCCGGTGAAGACTGTGAAGTTTGTGGTTCTCCTATGGT
TATAAAAATGGGACGCTATGGTAAGTTCATGGCTTGCTCAAACTTCCCGGATTGTCGTAATACAAAAGCGATAGTTAAGT
CTATTGGTGTTAAATGTCCAAAATGTAATGATGGTGACGTCGTAGAAAGAAAATCTAAAAAGAATCGTGTCTTTTATGGA
TGTTCGAAATATCCTGAATGCGACTTTATCTCTTGGGATAAGCCGATTGGAAGAGATTGTCCAAAATGTAACCAATATCT
TGTTGAAAATAAAAAAGGCAAGACAACACAAGTAATATGTTCAAATTGCGATTATAAAGAGGCAGCGCAGAAATAA

Upstream 100 bases:

>100_bases
AAATAGACAGGGAAAATGATTTGTTTAGATATAAAACGTTGACAAAAGCAAAATTAAGCGTTTATCATTTATCTTTAGTA
ATTAGATTAGCGAGGGGGAA

Downstream 100 bases:

>100_bases
TATTTTTATTCCCTAGAGACATTTTAAGATTGTTAAATAGAATCATTAGTGAATCTTATTTTAAAGATAGTAATGGATTA
ATCTAAATAAGTGCGGATAA

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 691; Mature: 690

Protein sequence:

>691_residues
MTLADNLVIVESPAKAKTIEKYLGKKYKVIASMGHVRDLPRSQMGVDTEDNYEPKYITIRGKGPVVKELKKHAKKAKNVF
LASDPDREGEAIAWHLSKILELEDSKENRVVFNEITKDAVKESFKNPREIEMNLVDAQQARRILDRLVGYNISPVLWKKV
KKGLSAGRVQSVALRLVIDRENEIRNFKPEEYWTIEGEFRYKKSKFNAKFLHYKNKPFKLKTKKDVEKITAALDGDQFEI
TNVTKKEKTRNPANPFTTSTLQQEAARKLNFKARKTMMVAQQLYEGIDLKKQGTIGLITYMRTDSTRISDTAKAEAKQYI
TDKYGESYTSKRKASGKQGDQDAHEAIRPSSTMRTPDDMKSFLTKDQYRLYKLIWERFVASQMAPAILDTVSLDITQGDI
KFRANGQTIKFKGFMTLYVETKDDSDSEKENKLPKLEQGDKVTATQIEPAQHYTQPPPRYTEARLVKTLEELKIGRPSTY
APTIDTIQKRNYVKLESKRFVPTELGEIVHEQVKEYFPEIIDVEFTVNMETLLDKIAEGDITWRKVIDGFFSSFKQDVER
AEEEMEKIEIKDEPAGEDCEVCGSPMVIKMGRYGKFMACSNFPDCRNTKAIVKSIGVKCPKCNDGDVVERKSKKNRVFYG
CSKYPECDFISWDKPIGRDCPKCNQYLVENKKGKTTQVICSNCDYKEAAQK

Sequences:

>Translated_691_residues
MTLADNLVIVESPAKAKTIEKYLGKKYKVIASMGHVRDLPRSQMGVDTEDNYEPKYITIRGKGPVVKELKKHAKKAKNVF
LASDPDREGEAIAWHLSKILELEDSKENRVVFNEITKDAVKESFKNPREIEMNLVDAQQARRILDRLVGYNISPVLWKKV
KKGLSAGRVQSVALRLVIDRENEIRNFKPEEYWTIEGEFRYKKSKFNAKFLHYKNKPFKLKTKKDVEKITAALDGDQFEI
TNVTKKEKTRNPANPFTTSTLQQEAARKLNFKARKTMMVAQQLYEGIDLKKQGTIGLITYMRTDSTRISDTAKAEAKQYI
TDKYGESYTSKRKASGKQGDQDAHEAIRPSSTMRTPDDMKSFLTKDQYRLYKLIWERFVASQMAPAILDTVSLDITQGDI
KFRANGQTIKFKGFMTLYVETKDDSDSEKENKLPKLEQGDKVTATQIEPAQHYTQPPPRYTEARLVKTLEELKIGRPSTY
APTIDTIQKRNYVKLESKRFVPTELGEIVHEQVKEYFPEIIDVEFTVNMETLLDKIAEGDITWRKVIDGFFSSFKQDVER
AEEEMEKIEIKDEPAGEDCEVCGSPMVIKMGRYGKFMACSNFPDCRNTKAIVKSIGVKCPKCNDGDVVERKSKKNRVFYG
CSKYPECDFISWDKPIGRDCPKCNQYLVENKKGKTTQVICSNCDYKEAAQK
>Mature_690_residues
TLADNLVIVESPAKAKTIEKYLGKKYKVIASMGHVRDLPRSQMGVDTEDNYEPKYITIRGKGPVVKELKKHAKKAKNVFL
ASDPDREGEAIAWHLSKILELEDSKENRVVFNEITKDAVKESFKNPREIEMNLVDAQQARRILDRLVGYNISPVLWKKVK
KGLSAGRVQSVALRLVIDRENEIRNFKPEEYWTIEGEFRYKKSKFNAKFLHYKNKPFKLKTKKDVEKITAALDGDQFEIT
NVTKKEKTRNPANPFTTSTLQQEAARKLNFKARKTMMVAQQLYEGIDLKKQGTIGLITYMRTDSTRISDTAKAEAKQYIT
DKYGESYTSKRKASGKQGDQDAHEAIRPSSTMRTPDDMKSFLTKDQYRLYKLIWERFVASQMAPAILDTVSLDITQGDIK
FRANGQTIKFKGFMTLYVETKDDSDSEKENKLPKLEQGDKVTATQIEPAQHYTQPPPRYTEARLVKTLEELKIGRPSTYA
PTIDTIQKRNYVKLESKRFVPTELGEIVHEQVKEYFPEIIDVEFTVNMETLLDKIAEGDITWRKVIDGFFSSFKQDVERA
EEEMEKIEIKDEPAGEDCEVCGSPMVIKMGRYGKFMACSNFPDCRNTKAIVKSIGVKCPKCNDGDVVERKSKKNRVFYGC
SKYPECDFISWDKPIGRDCPKCNQYLVENKKGKTTQVICSNCDYKEAAQK

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=630, Percent_Identity=25.5555555555556, Blast_Score=139, Evalue=8e-33,
Organism=Homo sapiens, GI4507635, Length=556, Percent_Identity=24.1007194244604, Blast_Score=122, Evalue=1e-27,
Organism=Escherichia coli, GI1787529, Length=753, Percent_Identity=38.7782204515272, Blast_Score=520, Evalue=1e-149,
Organism=Escherichia coli, GI1788061, Length=632, Percent_Identity=22.4683544303797, Blast_Score=115, Evalue=1e-26,
Organism=Escherichia coli, GI48994931, Length=110, Percent_Identity=36.3636363636364, Blast_Score=100, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI32563869, Length=588, Percent_Identity=25.8503401360544, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17555378, Length=504, Percent_Identity=23.4126984126984, Blast_Score=115, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6323263, Length=503, Percent_Identity=23.6580516898608, Blast_Score=117, Evalue=8e-27,
Organism=Drosophila melanogaster, GI24585251, Length=629, Percent_Identity=23.2114467408585, Blast_Score=144, Evalue=3e-34,
Organism=Drosophila melanogaster, GI24640096, Length=519, Percent_Identity=23.121387283237, Blast_Score=103, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 79286; Mature: 79154

Theoretical pI: Translated: 9.49; Mature: 9.49

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLADNLVIVESPAKAKTIEKYLGKKYKVIASMGHVRDLPRSQMGVDTEDNYEPKYITIR
CCCCCCEEEEECCHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCEEEEE
GKGPVVKELKKHAKKAKNVFLASDPDREGEAIAWHLSKILELEDSKENRVVFNEITKDAV
CCCCHHHHHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
KESFKNPREIEMNLVDAQQARRILDRLVGYNISPVLWKKVKKGLSAGRVQSVALRLVIDR
HHHHCCCCEEEEEHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHEEEEEEECC
ENEIRNFKPEEYWTIEGEFRYKKSKFNAKFLHYKNKPFKLKTKKDVEKITAALDGDQFEI
CHHHCCCCCCCEEEECCEEEEECCCCCEEEEEECCCCEEECCHHHHHHHHHHHCCCCEEE
TNVTKKEKTRNPANPFTTSTLQQEAARKLNFKARKTMMVAQQLYEGIDLKKQGTIGLITY
ECCHHHHHCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEE
MRTDSTRISDTAKAEAKQYITDKYGESYTSKRKASGKQGDQDAHEAIRPSSTMRTPDDMK
EECCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHCCCCCCCCCHHHHH
SFLTKDQYRLYKLIWERFVASQMAPAILDTVSLDITQGDIKFRANGQTIKFKGFMTLYVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCEEEEECCCEEEEEEEEEEEEE
TKDDSDSEKENKLPKLEQGDKVTATQIEPAQHYTQPPPRYTEARLVKTLEELKIGRPSTY
ECCCCCCHHHHCCCCCCCCCCEEEEECCHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCC
APTIDTIQKRNYVKLESKRFVPTELGEIVHEQVKEYFPEIIDVEFTVNMETLLDKIAEGD
CCCHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHCHHHEEEEEEECHHHHHHHHHCCC
ITWRKVIDGFFSSFKQDVERAEEEMEKIEIKDEPAGEDCEVCGSPMVIKMGRYGKFMACS
CHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCHHHCCCCEEEEECCCCCEEEEC
NFPDCRNTKAIVKSIGVKCPKCNDGDVVERKSKKNRVFYGCSKYPECDFISWDKPIGRDC
CCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCCCC
PKCNQYLVENKKGKTTQVICSNCDYKEAAQK
CHHHHHHHCCCCCCEEEEEECCCCHHHHHCC
>Mature Secondary Structure 
TLADNLVIVESPAKAKTIEKYLGKKYKVIASMGHVRDLPRSQMGVDTEDNYEPKYITIR
CCCCCEEEEECCHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCEEEEE
GKGPVVKELKKHAKKAKNVFLASDPDREGEAIAWHLSKILELEDSKENRVVFNEITKDAV
CCCCHHHHHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
KESFKNPREIEMNLVDAQQARRILDRLVGYNISPVLWKKVKKGLSAGRVQSVALRLVIDR
HHHHCCCCEEEEEHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHEEEEEEECC
ENEIRNFKPEEYWTIEGEFRYKKSKFNAKFLHYKNKPFKLKTKKDVEKITAALDGDQFEI
CHHHCCCCCCCEEEECCEEEEECCCCCEEEEEECCCCEEECCHHHHHHHHHHHCCCCEEE
TNVTKKEKTRNPANPFTTSTLQQEAARKLNFKARKTMMVAQQLYEGIDLKKQGTIGLITY
ECCHHHHHCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEE
MRTDSTRISDTAKAEAKQYITDKYGESYTSKRKASGKQGDQDAHEAIRPSSTMRTPDDMK
EECCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHCCCCCCCCCHHHHH
SFLTKDQYRLYKLIWERFVASQMAPAILDTVSLDITQGDIKFRANGQTIKFKGFMTLYVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCEEEEECCCEEEEEEEEEEEEE
TKDDSDSEKENKLPKLEQGDKVTATQIEPAQHYTQPPPRYTEARLVKTLEELKIGRPSTY
ECCCCCCHHHHCCCCCCCCCCEEEEECCHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCC
APTIDTIQKRNYVKLESKRFVPTELGEIVHEQVKEYFPEIIDVEFTVNMETLLDKIAEGD
CCCHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHCHHHEEEEEEECHHHHHHHHHCCC
ITWRKVIDGFFSSFKQDVERAEEEMEKIEIKDEPAGEDCEVCGSPMVIKMGRYGKFMACS
CHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCHHHCCCCEEEEECCCCCEEEEC
NFPDCRNTKAIVKSIGVKCPKCNDGDVVERKSKKNRVFYGCSKYPECDFISWDKPIGRDC
CCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCCCC
PKCNQYLVENKKGKTTQVICSNCDYKEAAQK
CHHHHHHHCCCCCCEEEEEECCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA