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The map label for this gene is def [C]

Identifier: 148267706

GI number: 148267706

Start: 1322398

End: 1322886

Strand: Direct

Name: def [C]

Synonym: SaurJH9_1274

Alternate gene names: 148267706

Gene position: 1322398-1322886 (Clockwise)

Preceding gene: 148267704

Following gene: 148267707

Centisome position: 45.49

GC content: 33.33

Gene sequence:

>489_bases
ATGGCGATTAAAAAGTTAGTACCAGCATCGCATCCTATTTTAACGAAAAAAGCGCAAGCAGTTAAAACATTTGATGATTC
GTTAAAAAGATTATTACAAGATTTAGAAGATACAATGTATGCACAAGAAGCTGCTGGCTTATGTGCACCTCAAATTAATC
AGTCATTGCAAGTGGCAATTATTGATATGGAAATGGAAGGATTATTACAACTTGTTAATCCGAAAATTATTAGTCAATCA
AATGAAACAATAACAGACTTAGAAGGTTCAATTACATTGCCAGATGTTTACGGCGAAGTGACAAGAAGTAAAATGATAGT
TGTTGAAAGTTATGACGTCAATGGGAACAAAGTTGAACTAACTGCACATGAAGATGTAGCAAGAATGATTTTGCATATTA
TAGATCAAATGAACGGTATCCCTTTTACAGAACGTGCGGACCGTATTTTAACAGATAAAGAAGTGGAGGCATATTTTATA
AATGACTAA

Upstream 100 bases:

>100_bases
AATCTTCAAATTGAAAAATATACATTCTATGATGTAAGGTCGCATTTTTAATATATTTACGTTATAATAGTTTGATGAAT
TTTATTGAGGAGTAAGAGTT

Downstream 100 bases:

>100_bases
AATAATATTTATGGGTACACCAGACTTTTCAACAACTGTTTTAGAAATGCTTATTGCAGAACATGATGTCATTGCAGTCG
TAACGCAACCAGATCGACCT

Product: peptide deformylase

Products: Formate; L-Methionine. [C]

Alternate protein names: Polypeptide deformylase-like

Number of amino acids: Translated: 162; Mature: 161

Protein sequence:

>162_residues
MAIKKLVPASHPILTKKAQAVKTFDDSLKRLLQDLEDTMYAQEAAGLCAPQINQSLQVAIIDMEMEGLLQLVNPKIISQS
NETITDLEGSITLPDVYGEVTRSKMIVVESYDVNGNKVELTAHEDVARMILHIIDQMNGIPFTERADRILTDKEVEAYFI
ND

Sequences:

>Translated_162_residues
MAIKKLVPASHPILTKKAQAVKTFDDSLKRLLQDLEDTMYAQEAAGLCAPQINQSLQVAIIDMEMEGLLQLVNPKIISQS
NETITDLEGSITLPDVYGEVTRSKMIVVESYDVNGNKVELTAHEDVARMILHIIDQMNGIPFTERADRILTDKEVEAYFI
ND
>Mature_161_residues
AIKKLVPASHPILTKKAQAVKTFDDSLKRLLQDLEDTMYAQEAAGLCAPQINQSLQVAIIDMEMEGLLQLVNPKIISQSN
ETITDLEGSITLPDVYGEVTRSKMIVVESYDVNGNKVELTAHEDVARMILHIIDQMNGIPFTERADRILTDKEVEAYFIN
D

Specific function: Removes The Formyl Group From The N-Terminal Met Of Newly Synthesized Proteins. Requires At Least A Dipeptide For An Efficient Rate Of Reaction. N-Terminal L-Methionine Is A Prerequisite For Activity But The Enzyme Has Broad Specificity At Other Positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family

Homologues:

Organism=Escherichia coli, GI1789682, Length=131, Percent_Identity=30.5343511450382, Blast_Score=65, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DEFL_STAAC (Q5HGL7)

Other databases:

- EMBL:   CP000046
- RefSeq:   YP_186090.1
- ProteinModelPortal:   Q5HGL7
- SMR:   Q5HGL7
- STRING:   Q5HGL7
- EnsemblBacteria:   EBSTAT00000009980
- GeneID:   3237852
- GenomeReviews:   CP000046_GR
- KEGG:   sac:SACOL1227
- TIGR:   SACOL1227
- eggNOG:   COG0242
- GeneTree:   EBGT00050000024244
- HOGENOM:   HBG665227
- OMA:   YGEVERS
- ProtClustDB:   PRK14595
- BioCyc:   SAUR93062:SACOL1227-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00163
- InterPro:   IPR000181
- Gene3D:   G3DSA:3.90.45.10
- PANTHER:   PTHR10458
- PIRSF:   PIRSF004749
- PRINTS:   PR01576

Pfam domain/function: PF01327 Pep_deformylase; SSF56420 Fmet_deformylase

EC number: 3.5.1.88 [C]

Molecular weight: Translated: 18102; Mature: 17971

Theoretical pI: Translated: 4.37; Mature: 4.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIKKLVPASHPILTKKAQAVKTFDDSLKRLLQDLEDTMYAQEAAGLCAPQINQSLQVAI
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEE
IDMEMEGLLQLVNPKIISQSNETITDLEGSITLPDVYGEVTRSKMIVVESYDVNGNKVEL
EEECHHHHHHHHCCHHCCCCCCEEECCCCCEECCHHHHHHCCCEEEEEEEECCCCCEEEE
TAHEDVARMILHIIDQMNGIPFTERADRILTDKEVEAYFIND
EEHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCEEEEECC
>Mature Secondary Structure 
AIKKLVPASHPILTKKAQAVKTFDDSLKRLLQDLEDTMYAQEAAGLCAPQINQSLQVAI
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEE
IDMEMEGLLQLVNPKIISQSNETITDLEGSITLPDVYGEVTRSKMIVVESYDVNGNKVEL
EEECHHHHHHHHCCHHCCCCCCEEECCCCCEECCHHHHHHCCCEEEEEEEECCCCCEEEE
TAHEDVARMILHIIDQMNGIPFTERADRILTDKEVEAYFIND
EEHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-Formyl-L-Methionine; H2O [C]

Specific reaction: N-Formyl-L-Methionine + H2O = Formate + L-Methionine. [C]

General reaction: Carboxylic acid amide hydrolysis [C]

Inhibitor: Dithiothreitol; EDTA; o-Phenantroline [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA