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The map label for this gene is pyrB

Identifier: 148267691

GI number: 148267691

Start: 1304441

End: 1305322

Strand: Direct

Name: pyrB

Synonym: SaurJH9_1259

Alternate gene names: 148267691

Gene position: 1304441-1305322 (Clockwise)

Preceding gene: 148267690

Following gene: 148267692

Centisome position: 44.88

GC content: 31.63

Gene sequence:

>882_bases
ATGAATCATTTATTATCAATGGAACATTTATCTACAGATCAAATATACAAACTTATCCAAAAGGCAAGTCAATTTAAATC
TGGTGAACGTCAACTACCAAACTTTGAAGGGAAATATGTCGCAAATTTATTCTTTGAAAATTCTACTCGAACAAAATGTA
GTTTTGAAATGGCAGAACTTAAGCTAGGGTTAAAAACGATTAGCTTTGAAACATCAACATCATCTGTTTCAAAAGGTGAA
TCTTTATATGACACATGTAAAACTTTAGAAAGTATTGGCTGTGATTTATTAGTCATTAGACATCCGTTTAATAACTACTA
TGAAAAATTAGCGAATATTAACATCCCAATTGCGAATGCTGGTGATGGTAGTGGACAACATCCAACACAAAGTTTACTTG
ATTTAATGACGATATATGAAGAATATGGATATTTTGAAGGCTTGAATGTATTGATTTGTGGAGACATTAAAAATTCACGT
GTCGCACGTAGTAATTACCATAGTTTAAAAGCATTAGGTGCAAACGTAATGTTTAATAGCCCAAATGCTTGGATTGATGA
TTCTTTAGAAGCACCTTATGTAAATATAGATGATGTTATAGAAACAGTAGATATAGTTATGTTATTAAGAATTCAACATG
AAAGACATGGGCTTGCAGAAGAAACTAGATTTGCAGCAGATGATTATCATCAAAAGCATGGCTTAAATGAAGTGCGCTAT
AACAAATTACAAGAACATGCTATTGTTATGCATCCGGCACCTGTGAATAGAGGAGTAGAAATACAAAGCGATTTAGTAGA
AGCTTCAAAATCAAGAATTTTTAAGCAAATGGAAAATGGCGTTTACTTAAGAATGGCAGTCATTGATGAATTATTAAAAT
AG

Upstream 100 bases:

>100_bases
GATGGCATTAGCTGCACTTTCAGGAATTATTTTGAACTTAATCTTACCTAAAGAGAAAAAACAAAACAATTAAGATTTAC
AAATTAAGGAGGGCGCTTTT

Downstream 100 bases:

>100_bases
GTAAGGGGACGAAAATGATGAAATTAATTAAAAACGGTAAAGTATTACAAAATGGAGAATTACAACAAGCAGATATTTTA
ATTGATGGTAAGGTAATTAA

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MNHLLSMEHLSTDQIYKLIQKASQFKSGERQLPNFEGKYVANLFFENSTRTKCSFEMAELKLGLKTISFETSTSSVSKGE
SLYDTCKTLESIGCDLLVIRHPFNNYYEKLANINIPIANAGDGSGQHPTQSLLDLMTIYEEYGYFEGLNVLICGDIKNSR
VARSNYHSLKALGANVMFNSPNAWIDDSLEAPYVNIDDVIETVDIVMLLRIQHERHGLAEETRFAADDYHQKHGLNEVRY
NKLQEHAIVMHPAPVNRGVEIQSDLVEASKSRIFKQMENGVYLRMAVIDELLK

Sequences:

>Translated_293_residues
MNHLLSMEHLSTDQIYKLIQKASQFKSGERQLPNFEGKYVANLFFENSTRTKCSFEMAELKLGLKTISFETSTSSVSKGE
SLYDTCKTLESIGCDLLVIRHPFNNYYEKLANINIPIANAGDGSGQHPTQSLLDLMTIYEEYGYFEGLNVLICGDIKNSR
VARSNYHSLKALGANVMFNSPNAWIDDSLEAPYVNIDDVIETVDIVMLLRIQHERHGLAEETRFAADDYHQKHGLNEVRY
NKLQEHAIVMHPAPVNRGVEIQSDLVEASKSRIFKQMENGVYLRMAVIDELLK
>Mature_293_residues
MNHLLSMEHLSTDQIYKLIQKASQFKSGERQLPNFEGKYVANLFFENSTRTKCSFEMAELKLGLKTISFETSTSSVSKGE
SLYDTCKTLESIGCDLLVIRHPFNNYYEKLANINIPIANAGDGSGQHPTQSLLDLMTIYEEYGYFEGLNVLICGDIKNSR
VARSNYHSLKALGANVMFNSPNAWIDDSLEAPYVNIDDVIETVDIVMLLRIQHERHGLAEETRFAADDYHQKHGLNEVRY
NKLQEHAIVMHPAPVNRGVEIQSDLVEASKSRIFKQMENGVYLRMAVIDELLK

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI18105007, Length=313, Percent_Identity=33.8658146964856, Blast_Score=159, Evalue=2e-39,
Organism=Escherichia coli, GI2367364, Length=303, Percent_Identity=33.993399339934, Blast_Score=141, Evalue=6e-35,
Organism=Escherichia coli, GI1786469, Length=196, Percent_Identity=29.5918367346939, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1790703, Length=194, Percent_Identity=27.8350515463918, Blast_Score=65, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI193204318, Length=324, Percent_Identity=32.7160493827161, Blast_Score=141, Evalue=5e-34,
Organism=Saccharomyces cerevisiae, GI6322331, Length=306, Percent_Identity=30.3921568627451, Blast_Score=122, Evalue=5e-29,
Organism=Drosophila melanogaster, GI24642586, Length=310, Percent_Identity=34.5161290322581, Blast_Score=160, Evalue=1e-39,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PYRB_STAA1 (A7X1E9)

Other databases:

- EMBL:   AP009324
- RefSeq:   YP_001441780.1
- ProteinModelPortal:   A7X1E9
- SMR:   A7X1E9
- STRING:   A7X1E9
- EnsemblBacteria:   EBSTAT00000003504
- GeneID:   5559557
- GenomeReviews:   AP009324_GR
- KEGG:   saw:SAHV_1190
- eggNOG:   COG0540
- GeneTree:   EBGT00050000023906
- HOGENOM:   HBG579429
- OMA:   MNRGLEI
- ProtClustDB:   PRK00856
- BioCyc:   SAUR418127:SAHV_1190-MONOMER
- HAMAP:   MF_00001
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082
- PRINTS:   PR00100
- PRINTS:   PR00101
- TIGRFAMs:   TIGR00670

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.2

Molecular weight: Translated: 33258; Mature: 33258

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHLLSMEHLSTDQIYKLIQKASQFKSGERQLPNFEGKYVANLFFENSTRTKCSFEMAEL
CCCCCCHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHH
KLGLKTISFETSTSSVSKGESLYDTCKTLESIGCDLLVIRHPFNNYYEKLANINIPIANA
HHHHHEEEECCCCCHHHCCHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHCCCCEEECC
GDGSGQHPTQSLLDLMTIYEEYGYFEGLNVLICGDIKNSRVARSNYHSLKALGANVMFNS
CCCCCCCHHHHHHHHHHHHHHHCHHCCCCEEEECCCCCCCHHHHHHHHHHHHCCEEEECC
PNAWIDDSLEAPYVNIDDVIETVDIVMLLRIQHERHGLAEETRFAADDYHQKHGLNEVRY
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHH
NKLQEHAIVMHPAPVNRGVEIQSDLVEASKSRIFKQMENGVYLRMAVIDELLK
HHHHHCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHC
>Mature Secondary Structure
MNHLLSMEHLSTDQIYKLIQKASQFKSGERQLPNFEGKYVANLFFENSTRTKCSFEMAEL
CCCCCCHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHH
KLGLKTISFETSTSSVSKGESLYDTCKTLESIGCDLLVIRHPFNNYYEKLANINIPIANA
HHHHHEEEECCCCCHHHCCHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHCCCCEEECC
GDGSGQHPTQSLLDLMTIYEEYGYFEGLNVLICGDIKNSRVARSNYHSLKALGANVMFNS
CCCCCCCHHHHHHHHHHHHHHHCHHCCCCEEEECCCCCCCHHHHHHHHHHHHCCEEEECC
PNAWIDDSLEAPYVNIDDVIETVDIVMLLRIQHERHGLAEETRFAADDYHQKHGLNEVRY
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHH
NKLQEHAIVMHPAPVNRGVEIQSDLVEASKSRIFKQMENGVYLRMAVIDELLK
HHHHHCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA