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The map label for this gene is leuA [H]

Identifier: 148267410

GI number: 148267410

Start: 1029430

End: 1030575

Strand: Direct

Name: leuA [H]

Synonym: SaurJH9_0976

Alternate gene names: 148267410

Gene position: 1029430-1030575 (Clockwise)

Preceding gene: 148267408

Following gene: 148267411

Centisome position: 35.42

GC content: 26.88

Gene sequence:

>1146_bases
ATGATTCGAATTCAAGATAATACAATAAGGGATGGTATGCAACAAAGTAATGTTCGAAAAAGTCTAATTATAAAAAAAGA
AGTATTGAAACAAATTAACAAGTTAAATATAAATTCTGTTGAAGTAGGCATGTGTACAACTATCGAGGATGAATTTAATA
TTCATCAATTCAGAGACATTTTAAGTCCTGAAAAAGAATTAGTAGTATTGACTAGGCTTAATGAAAAAGAAATAAAAAAA
ATAGTCAAATTGAAAATTCATAATTTAGTGGTAAAAATACTATTGCCAATATCTGACTTGCATATAAAAGAAAAGCTTAA
TTTTTCAAATAAATATTATATTCAGAAAATCAAAGACTGCTTGGATATATTAAAGAAAGATAAAAAAGGAGTAGATATTT
GTTTTGAAGATGCAACAAGGACTTCTAGAGAAAAATTGAAAGAATACATGGAAATTATTTCAAAATATCAAGTTAGAACA
GTTACATTTGCGGACACTGTAGGATGTTCGACACCATTAGAATACGGAGATATTTTTAATTACTTTGTAAAAAAATATTC
TAACATAATTTTTTCTGCTCATTGTCATAACGATCTAGGGTTGGCTACTGCAAATACATTAGCTGCAATTTTAAATGGTG
CAAAGCAAATAGAAACTACATTTTTGGGAATTGGTGAGAGAGCGGGTAATGCTCCTATTGAGGAAATAATTACTATTTTG
ACAAAAAAACAAATAGAAAGTACGGAATTCACTTTACCCGACGTATATAAAACTAGTATTAATATTTCTAAAATTCTCGA
TTTTCAAATATCAGAAAACAAACCTATAATTGGTGAAAATATATTTAAACATGAATCAGGAATTCATCAAGATGGTACTA
AAAAAAATATAAATATGTATCAATATTTAGTTCCTAGTGATTTAGGATTGAAAAATTCACAAGTTGTTCAGTTTCCAATA
AGTAATATTTCTAGTAAGAAAATCTTGCACAATAAATTTAAATCAATAGTTAACACTGAAGAAATTGATGAAAATATTTC
TTTCTATAAACTTGTGAACAAGTTTTCACCTGAAGTAGCACCTGAAGATACAGTGGATTTACTTCAGATAATAAAAAGGA
GGAGTAAAGATGGAAATTTTAAATGA

Upstream 100 bases:

>100_bases
TCTTTCAAAAAAGTTAATAATTCATTAGATAATATTTAATTTTTATTGCATAAAAATAATATTAATATTATATCAGAGGT
TATCGAGGGGGTGAAGTAAT

Downstream 100 bases:

>100_bases
TTCTGTAAAAAGTTTTGAAAAACTTTATTTACAATTACAAGATGGTTTTCCAGTGATTGTTCCTACTGATACTAATTATA
ATTTATGTAGTTTACCAAAC

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 381; Mature: 381

Protein sequence:

>381_residues
MIRIQDNTIRDGMQQSNVRKSLIIKKEVLKQINKLNINSVEVGMCTTIEDEFNIHQFRDILSPEKELVVLTRLNEKEIKK
IVKLKIHNLVVKILLPISDLHIKEKLNFSNKYYIQKIKDCLDILKKDKKGVDICFEDATRTSREKLKEYMEIISKYQVRT
VTFADTVGCSTPLEYGDIFNYFVKKYSNIIFSAHCHNDLGLATANTLAAILNGAKQIETTFLGIGERAGNAPIEEIITIL
TKKQIESTEFTLPDVYKTSINISKILDFQISENKPIIGENIFKHESGIHQDGTKKNINMYQYLVPSDLGLKNSQVVQFPI
SNISSKKILHNKFKSIVNTEEIDENISFYKLVNKFSPEVAPEDTVDLLQIIKRRSKDGNFK

Sequences:

>Translated_381_residues
MIRIQDNTIRDGMQQSNVRKSLIIKKEVLKQINKLNINSVEVGMCTTIEDEFNIHQFRDILSPEKELVVLTRLNEKEIKK
IVKLKIHNLVVKILLPISDLHIKEKLNFSNKYYIQKIKDCLDILKKDKKGVDICFEDATRTSREKLKEYMEIISKYQVRT
VTFADTVGCSTPLEYGDIFNYFVKKYSNIIFSAHCHNDLGLATANTLAAILNGAKQIETTFLGIGERAGNAPIEEIITIL
TKKQIESTEFTLPDVYKTSINISKILDFQISENKPIIGENIFKHESGIHQDGTKKNINMYQYLVPSDLGLKNSQVVQFPI
SNISSKKILHNKFKSIVNTEEIDENISFYKLVNKFSPEVAPEDTVDLLQIIKRRSKDGNFK
>Mature_381_residues
MIRIQDNTIRDGMQQSNVRKSLIIKKEVLKQINKLNINSVEVGMCTTIEDEFNIHQFRDILSPEKELVVLTRLNEKEIKK
IVKLKIHNLVVKILLPISDLHIKEKLNFSNKYYIQKIKDCLDILKKDKKGVDICFEDATRTSREKLKEYMEIISKYQVRT
VTFADTVGCSTPLEYGDIFNYFVKKYSNIIFSAHCHNDLGLATANTLAAILNGAKQIETTFLGIGERAGNAPIEEIITIL
TKKQIESTEFTLPDVYKTSINISKILDFQISENKPIIGENIFKHESGIHQDGTKKNINMYQYLVPSDLGLKNSQVVQFPI
SNISSKKILHNKFKSIVNTEEIDENISFYKLVNKFSPEVAPEDTVDLLQIIKRRSKDGNFK

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=362, Percent_Identity=29.8342541436464, Blast_Score=151, Evalue=8e-38,
Organism=Saccharomyces cerevisiae, GI6320019, Length=195, Percent_Identity=31.7948717948718, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6320071, Length=187, Percent_Identity=31.0160427807487, Blast_Score=91, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6324225, Length=321, Percent_Identity=23.6760124610592, Blast_Score=74, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6324682, Length=321, Percent_Identity=24.6105919003115, Blast_Score=73, Evalue=7e-14,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 43689; Mature: 43689

Theoretical pI: Translated: 9.16; Mature: 9.16

Prosite motif: PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRIQDNTIRDGMQQSNVRKSLIIKKEVLKQINKLNINSVEVGMCTTIEDEFNIHQFRDI
CEEECCCHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCHHHHHHH
LSPEKELVVLTRLNEKEIKKIVKLKIHNLVVKILLPISDLHIKEKLNFSNKYYIQKIKDC
HCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
LDILKKDKKGVDICFEDATRTSREKLKEYMEIISKYQVRTVTFADTVGCSTPLEYGDIFN
HHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHEEEEEEEHHCCCCCCCCHHHHHH
YFVKKYSNIIFSAHCHNDLGLATANTLAAILNGAKQIETTFLGIGERAGNAPIEEIITIL
HHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
TKKQIESTEFTLPDVYKTSINISKILDFQISENKPIIGENIFKHESGIHQDGTKKNINMY
HHHHHCCCCCCCCCHHHHCCCHHHHEEEEECCCCCCCCCHHHHHCCCCCCCCCCCCCCEE
QYLVPSDLGLKNSQVVQFPISNISSKKILHNKFKSIVNTEEIDENISFYKLVNKFSPEVA
EEECCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCHHHHHCCHHHHHHHHHHCCCCC
PEDTVDLLQIIKRRSKDGNFK
CCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MIRIQDNTIRDGMQQSNVRKSLIIKKEVLKQINKLNINSVEVGMCTTIEDEFNIHQFRDI
CEEECCCHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCHHHHHHH
LSPEKELVVLTRLNEKEIKKIVKLKIHNLVVKILLPISDLHIKEKLNFSNKYYIQKIKDC
HCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
LDILKKDKKGVDICFEDATRTSREKLKEYMEIISKYQVRTVTFADTVGCSTPLEYGDIFN
HHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHEEEEEEEHHCCCCCCCCHHHHHH
YFVKKYSNIIFSAHCHNDLGLATANTLAAILNGAKQIETTFLGIGERAGNAPIEEIITIL
HHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
TKKQIESTEFTLPDVYKTSINISKILDFQISENKPIIGENIFKHESGIHQDGTKKNINMY
HHHHHCCCCCCCCCHHHHCCCHHHHEEEEECCCCCCCCCHHHHHCCCCCCCCCCCCCCEE
QYLVPSDLGLKNSQVVQFPISNISSKKILHNKFKSIVNTEEIDENISFYKLVNKFSPEVA
EEECCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCHHHHHCCHHHHHHHHHHCCCCC
PEDTVDLLQIIKRRSKDGNFK
CCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA